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Experiment Title: Millon's Test Objective

Millon's test is used to identify the amino acid tyrosine. Tyrosine contains a phenol group, which reacts with nitric acid in Millon's reagent to form a brick-red solution or precipitate. The procedure involves adding the sample to Millon's reagent and sodium nitrite, which will produce a red color if tyrosine is present, indicating a positive test result for the phenol-containing amino acid tyrosine.

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محمد الربيعي
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399 views

Experiment Title: Millon's Test Objective

Millon's test is used to identify the amino acid tyrosine. Tyrosine contains a phenol group, which reacts with nitric acid in Millon's reagent to form a brick-red solution or precipitate. The procedure involves adding the sample to Millon's reagent and sodium nitrite, which will produce a red color if tyrosine is present, indicating a positive test result for the phenol-containing amino acid tyrosine.

Uploaded by

محمد الربيعي
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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Al-Mashreq University Name: ‫علي عبد األمير علي‬

First stage /B2 Experiment NO: 4

Experiment Title: Millon's test

Objective:

This test is specific for tyrosine. Because it is the only amino acid containing a phenol group, a hydroxyl
group attached to benzene ring.

Tools and Materials:

Tools

Tube , Dropper , spatula, flask

Materials

nitric acid, water, amino acid containing a phenol group, sodium nitrite

Procedure:

The phenol group of tyrosine is first nitrated by nitric acid in the test solution. Then the nitrated tyrosine
complexes mercury ions in the solution to form a brick-red solution or precipitate of nitrated tyrosine, in
all cases, appearance of red color is positive test.

Method:

1. About 2 ml of the sample solution or the tyrosine solution is taken

in a test tube.

2. Add about 2 ml of Millon’s reagent in the test tube and kept in the

water bath for about 2 minutes.

3. Cool and add 2 drops of 1% of sodium nitrite solution .

4. Formation of red colored precipitatered observed immediately

Results

1. Millon’s test:

+ve test -- > red color precipitate --- > the sample is nitrated tyrosine

Discussion:

Amino acid containing a phenol group reacts with Millon’s reagent which give positive results by
changing the color of the solution to red.

Conclusion: Millon’s test gives a satisfactory solution to distinguish the properties of Amino acid
containing a phenol group.

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