TEST FOOD SUBSTANCES
Nutrient Reagents Procedure Positive Negative
result result
Starch Iodine solution Add a few drops Blue-black Yellow orange
to a sample of
food
Reducing Benedict’s Heat in boiling Color change No color change
sugars solution water bath with a from blue to –
(e.g. glucose and solution of the green, orange or Solution remains
fructose) food red blue
Nonreducing Hydrochloric Boil sample Color change No color change
sugars (e.g. acid (HCl), with HCl; cool; from blue to –
Sucrose) sodium neutralize with green, orange or Solution remains
hydroxide NaOH; test with red blue
(NaOH), Benedict’s
Benedict’s solution as above
solution
Protein Biuret solution – Add few drops of Color change No color change
a Biuret solution to from blue to lilac –
Solution of a solution of the or purple solution is blue
sodium sample
hydroxide
(NaOH)
and copper
sulfate
(CuSO4)
Lipids (fats and Water and Dissolve White No white
oils) ethanol sample in emulsion emulsion
ethanol, then
pour solution
into water
Filter paper Rub the food Greasy No greasy
sample into spot spot
the paper and which is
leave to dry translucent