SCIEX Triple Quad 5500+ LC-MS/MS

System—QTRAP Ready
Site Planning Guide

RUO-IDV-01-8462-D June 2021

This document is provided to customers who have purchased SCIEX equipment to use in the operation of
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Contents

1 Introduction..............................................................................................................................................5
Customer Site Planner Responsibilities...................................................................................................5
FSE Responsibilities................................................................................................................................6
During Installation...............................................................................................................................7
Customer Familiarization....................................................................................................................7
2 Site Planning Checklist............................................................................................................................9
Customer Information...............................................................................................................................9
Requirements.........................................................................................................................................10
Site Layout Requirements.................................................................................................................10
Electrical Requirements....................................................................................................................12
Gas Supply Requirements ...............................................................................................................14
Ventilation and Waste-Collection Requirements...............................................................................15
Computer, Network, and Software Requirements ...........................................................................15
Environmental Requirements............................................................................................................16
Solutions and Equipment Requirements..........................................................................................17
Product Familiarization.....................................................................................................................18
Additional Site Planning....................................................................................................................18
Comments and Exceptions....................................................................................................................19
Signoff....................................................................................................................................................19
A Site Requirements.................................................................................................................................20
Site Layout Requirements......................................................................................................................20
Laboratory Layout and Site Clearances...........................................................................................20
Weights and Dimension....................................................................................................................22
Electrical Requirements.........................................................................................................................24
Mains Supply Connections...............................................................................................................24
Mains Supply Fluctuations................................................................................................................27
Protective Earth Conductor...............................................................................................................27
Uninterruptible Power Supply or Power Conditioner........................................................................27
System Electrical Specifications.......................................................................................................28
Gas Supply Requirements.....................................................................................................................29
Optional Gas Generators..................................................................................................................32
Ventilation and Waste Collection Requirements....................................................................................32
Computer, Network, and Software Requirements..................................................................................35
Acquisition Computer Requirements................................................................................................35
Printer Requirements........................................................................................................................35
LAN Connection................................................................................................................................35
Software Requirements ...................................................................................................................36
Environmental Requirements.................................................................................................................36

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Contents

Sound Pressure Level.......................................................................................................................37

Vibration............................................................................................................................................37
BioSafety Requirements...................................................................................................................37
Solutions and Equipment Requirements................................................................................................37
Customer Familiarization..................................................................................................................38
Mass Spectrometer Operation .........................................................................................................38
B Equipment Safety Categories...............................................................................................................40
C Glossary of Symbols.............................................................................................................................41
Contact Us.................................................................................................................................................47
Customer Training..................................................................................................................................47
Online Learning Center..........................................................................................................................47
SCIEX Support.......................................................................................................................................47
CyberSecurity.........................................................................................................................................47
Documentation.......................................................................................................................................47

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Introduction 1
This guide is for the site planner, the individual responsible for preparing the facility for the
TM
installation of the SCIEX Triple Quad 5500+ System.
For safety and regulatory information, refer to the System User Guide, available at sciex.com.

Customer Site Planner Responsibilities

Complete the Site Planning Checklist on page 9, in consultation with Facilities and Services
Personnel (gas, electrical, ventilation, and information technology [IT]), and return it to the SCIEX
Field Service Employee (FSE) before the completion date. Refer to Signoff on page 19.

Note: If the site preparation tasks are not complete when the SCIEX Field Service Employee
(FSE) arrives, then the scheduled installation will be postponed.

Note: The FSE will follow up if the checklist is not received prior to the scheduled installation
date.

• Verify that adequate space and the required shipping or receiving facilities are available. Refer
to Site Layout Requirements on page 20.
• Provide all required electrical receptacles. Refer to Electrical Requirements on page 24.
• Provide all required gas supplies, including shut off valves and regulators, at the installation
point. Refer to Gas Supply Requirements on page 29.
• Provide and install all required vents and ventilation devices. Refer to Ventilation and Waste
Collection Requirements on page 32.
• Verify that the requirements for the computer and network are met. Refer to Computer, Network,
and Software Requirements on page 35.
• Provide a printer and an active, tested LAN connection. Refer to Computer, Network, and
Software Requirements on page 35.
• Verify that the requirements for the operating environment are met. Refer to Environmental
Requirements on page 36.
• Provide all required solutions and laboratory equipment, including all fittings, and sample tubing
for the liquid chromatography (LC) equipment, unless purchased from SCIEX. Refer to Solutions
and Equipment Requirements on page 37.

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Introduction

• Validate the customer account.

SCIEX sends an e-mail with the subject, "Please validate your account | Welcome to sciex.com".
Open the e-mail and follow the instructions to validate the account, and then go to SCIEX
University. Validation automatically registers the mass spectrometer for support, grants access
to productivity tools, and enrolls the customer in the appropriate courses at SCIEX University.

Note: If this e-mail has been lost or deleted, or to add users to the account, then contact
[email protected]. Validation is specific to the order, and must be performed even
if the customer already has an account on sciex.com.

• When the shipment arrives, inspect the packaging exterior for damage. If there is any damage,
or if the shock or tip sensors have been tripped, then note any issues on the delivery receipt
and notify SCIEX immediately.
• Contact SCIEX Customer Service or the local FSE to schedule the installation.
• Identify a primary learner who will participate in the Customer Familiarization. This individual
must complete the prerequisite Introduction to LC-MS/MS Operation Series for SCIEX Triple
Quadrupole and QTRAP Systems eLearning series, available from SCIEX University, and
download the course completion certificate.

Note: If the prelearning is not completed, then a demonstration will be substituted for the
hands-on portion of the Customer Familiarization. If the customer waives Customer
Familiarization, then the FSE only provides training on SCIEX Now resources.

• If available, identify a qualified maintenance person (QMP) who is suitably aware of the electrical
and chemical risks associated with servicing laboratory equipment. The FSE will review the
Qualified Maintenance Person Guide for the mass spectrometer with this person during the
Customer Familiarization process.

CAUTION: Potential System Damage. Do not unpack the system crates or boxes. The FSE
will unpack and help move the system at the time of the installation.

FSE Responsibilities
Note: If the site preparation tasks are not complete when the SCIEX Field Service Employee
(FSE) arrives, then the scheduled installation will be postponed.

• Review the checklist and discuss any outstanding issues with the site planner.

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 Introduction

• Supply all fittings, plugs, and cables required to connect the mass spectrometer to the electrical
receptacles and gas regulators. Electrical receptacles must be within the maximum distances
described in this document.
• Unpack, assemble, and set up any optional SCIEX benches ordered with the mass spectrometer.
• Unpack and set up the mass spectrometer.
• Unpack and set up the acquisition computer.
• Unpack and set up the optional LC equipment, sold and supported by SCIEX.
• Unpack and set up the optional gas generator equipment, sold by SCIEX.
• Unpack and set up the optional UPS equipment, sold by SCIEX.
• Test and qualify the system to the specifications in the Installation Checklist and Data Log.
• If approved by the customer, install the StatusScope Remote Monitoring Service on the
acquisition computer to enable remote, real-time monitoring of the status of the mass
spectrometer. Refer to the StatusScope Remote Monitoring Service Site Planning Guide.

During Installation

WARNING! Lifting Hazard. Use a mechanical lifting device to lift and move the mass
spectrometer. If the mass spectrometer must be moved manually, then at least six
people are required to move it safely. Follow established safe lifting procedures.
We recommend the use of a professional moving service. Refer to Weights and
Dimension on page 22 for the weights of system components.

The FSE unpacks the system, sets up the system, and then confirms its operation. When the
system reaches proper vacuum, the FSE conducts system performance tests.

Note: It takes several days for the FSE to set up the system and confirm operation.

Customer Familiarization
During installation, the FSE familiarizes the primary learner with the 5500+ system, and the primary
learner practices using the system by following the step-by-step exercise from the Introduction to
LC-MS/MS Operation Series for SCIEX Triple Quadrupole and QTRAP Systems eLearning series.
This eLearning series is available on SCIEX University at training.sciex.com. The Customer
Familiarization comprises the first phase of the learning experience for the primary learner. To
continue learning, the primary learner can log on to SCIEX University.

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Introduction

Note: Customer Familiarization provides hands-on training for the primary learner only. Up to
two additional users can be present for the training.

Note: To view personalized training information, including completed courses, assigned courses,
and certifications, log on to SCIEX University.

As a prerequisite to the hands-on training, the primary learner must have completed the Introduction
to LC-MS/MS Operation Series for SCIEX Triple Quadrupole and QTRAP Systems eLearning
series, and must show the course completion certificate to the FSE.

Note: If the prelearning is not completed, then a demonstration will be substituted for the hands-on
portion of the Customer Familiarization. If the customer waives Customer Familiarization, then
the FSE only provides training on SCIEX Now resources.

The format and extent of the Customer Familiarization depends on the customer configuration, as
follows:
• New installation of LC systems that are sold by SCIEX and are supported by the version of the
control software installed: The entire Customer Familiarization is completed, providing hands-on
familiarization to the primary learner. The results are saved on the acquisition computer.
• LC system not installed by SCIEX at the time of the mass spectrometer installation: The FSE
is not responsible for verifying the functionality of the LC system. The following topics are not
covered:
• Plumb the LC System and Load the Samples
• Build the Acquisition Method
• Create the Acquisition Batch
• Submit the Batch
Sample preparation procedures will be completed, and a set of example data will be used to
perform the data analysis.

Note: Familiarization for equipment supplied by other manufacturers is not provided by the SCIEX
FSE.

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Site Planning Checklist 2

Customer Information
Contact name

Organization

Address

City

State/Province/ ZIP code/Postal

Region code
Country

Telephone

E-mail address

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Site Planning Checklist

Requirements
Site Layout Requirements
Refer to Site Layout Requirements on page 20.

Requirement Complete N/A

The measured building clearances, including all external   —
doors, internal doors, and elevators, can accommodate
the equipment and crate dimensions.
If the requirements cannot be met, then contact a sales
or field service representative.

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 Site Planning Checklist

Mass Spectrometer Bench Requirements

Refer to Site Layout Requirements on page 20.

Requirement Complete N/A

A moveable or fixed bench that meets the dimension and   —
weight requirements of the system is provided for the
mass spectrometer. Refer to Weights and Dimension on
page 22.
If the bench is fixed, then there is 1 m (40 inches) of
clearance on all sides for service access. If the bench is
moveable, then there is 0.3 m (12 inches) of clearance
on all sides.

Note: If the bench is moveable, then

• Make sure that the bench is fixed during normal use.
• Make sure that the mass spectrometer can be moved
to a location with 1 m (40 inches) of clearance on all
sides, for service access.

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Site Planning Checklist

Electrical Requirements
Refer to Electrical Requirements on page 24.

Requirement Complete N/A

Installation of electrical supplies and fixtures complies with   —
local regulations and safety standards.
One branch circuit is provided for the mass spectrometer.   —
The mains supply outlet is less than 1.6 m (63 inches)
from the mass spectrometer. One outlet is required. The
outlet is accessible so that the mass spectrometer can be
disconnected in an emergency.
One branch circuit is provided for the roughing pump. The   —
mains supply outlet for the roughing pump is less than 1.6
m (63 inches) from the roughing pump. One outlet is
required.
One branch circuit is provided for the acquisition computer,   —
monitor, printer, and options. At least two outlets are
required, one for the acquisition computer and one for the
monitor.
For LC equipment and other options, contact the
manufacturer for more information.
(Optional) One branch circuit is provided for a standalone
   
gas generator with an air compressor. Contact the
manufacturer of the gas generator for more information.
The mains supply voltage does not fluctuate more than   —
±10% from the nominal voltage.

Note: Peripheral devices might have different mains

supply fluctuation limits. Confirm the mains supply
fluctuation limit with the manufacturer of each peripheral
device that will be used with the mass spectrometer.

The mains supply includes a correctly installed protective   —

earth conductor.

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 Site Planning Checklist

Requirement Complete N/A

(Optional) A customer-supplied UPS or power conditioner
   
is provided for the system. The UPS or power conditioner
must deliver 230 VAC, 50 Hz or 60 Hz, 4,000 VA
(minimum). Refer to Uninterruptible Power Supply or
Power Conditioner on page 27.

Note: SCIEX sells and supports several UPS power

protection units for mass spectrometer systems that are
custom configured to provide a total backup power
solution. Contact a SCIEX sales representative for more
information.

A qualified electrician has determined the appropriate   —

mains supply configuration, based on the system electrical
specifications. Refer to System Electrical Specifications
on page 28.

Electrical Requirements (International)

Refer to International Requirements on page 25.

Requirement Complete N/A

Electrical installations use locally approved standard
   
connections and cables.

Electrical Requirements (North America)

Refer to North American Requirements on page 26.

Requirement Complete N/A

Branch circuits for the mass spectrometer and roughing
   
pump are 15 A, 200 VAC to 240 VAC (208 VAC typical),
50 Hz or 60 Hz. Receptacles for these branch circuits are
equipped with CSA/NEMA 6-15R straight-blade
receptacles.
The branch circuit for the computer and monitor is 15 A,
   
100 VAC to 240 VAC (120 VAC typical), 50 Hz or 60 Hz.
For requirements for LC equipment, contact the
manufacturer.

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Site Planning Checklist

Gas Supply Requirements

Refer to Gas Supply Requirements on page 29.

Requirement Complete N/A

Installation of gas supplies and connections complies with   —
local regulations and safety standards.
Gas 1/Gas 2/bath gas/sheath gas is available:   —
• Zero-grade air, or a SCIEX-recommended gas
generator
• Delivery pressure of 100 psi (6.89 bar) minimum to
105 psi (7.25 bar) maximum, with flows up to 22 L/min.

Source exhaust gas is available:   —

• Clean, dry, and oil-free air, or ultra-high purity (UHP)

nitrogen (99.999%), or a SCIEX-recommended gas
generator
• Delivery pressure of 55 psi (3.79 bar) minimum to
60 psi (4.14 bar) maximum, with flows up to 25 L/min

Gas for the Curtain Gas Interface/bath gas/CAD gas is   —

available:
• UHP nitrogen, or a SCIEX-recommended gas generator
• Delivery pressure of 55 psi (3.79 bar) minimum to 60
psi (4.14 bar) maximum, with flows up to 15 L/min

(Optional) If a SCIEX mass spectrometer bench with a

   
built-in gas generator, or if a SCIEX-recommended
wall-mounted gas generator without a compressor is used,
then a supply of compressed oil-free air is provided.
Contact the manufacturer for more information.

Note: Under normal conditions, bath gas is taken from the same supply as the Gas 1 and Gas 2
flows.

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 Site Planning Checklist

Ventilation and Waste-Collection Requirements

Refer to Ventilation and Waste Collection Requirements on page 32.

Requirement Complete N/A

Installation of plumbing and ventilation fixtures complies   —
with local regulations and safety standards.
Ventilation of the laboratory environment in which the   —
system will be used complies with local regulations, and
the air exchange rate is appropriate for the work
performed.

Note: A minimum of 10 air exchanges/hour is required

for laboratory applications using toxic agents.

A negative flow vent is provided, with a total flow rate   —

capacity of 283 L/min (10 cfm), as measured at the inlet
to the laboratory ventilation system.
A smooth fitting is provided for the roughing pump, with   —
an outside diameter (o.d.) of 3.2 cm (1.25 inches). The
vent is within 1.5 m (60 inches) of the exhaust port on the
roughing pump, and at least 1 m (40 inches) above the
floor.
A fitting is provided for the source exhaust drain bottle,   —
with an o.d. of 2.5 cm (1 inch). The vent is within 1.5 m
(60 inches) of the source exhaust drain bottle.

Computer, Network, and Software Requirements

Refer to Computer, Network, and Software Requirements on page 35.

Requirement Complete N/A

A table is provided for the acquisition computer, located   —
within 3 m (120 inches) of the mass spectrometer.
A network computer name and domain password are   —
available.

Note: Complete this step before the acquisition software

is installed to prevent issues with the security database.

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Site Planning Checklist

Requirement Complete N/A

An active, tested LAN connection is available for the   —
acquisition computer, and a network or IT specialist is
available to help the FSE connect the computer to the
network.

Note: The LAN connection is required to activate the

Windows operating system license on the acquisition
computer, as well as to activate licenses for all SCIEX
software that will be installed.

If the StatusScope Remote Monitoring Service will be

   
installed, then the customer's IT specialist confirms that:
• Outbound connectivity is enabled using the HTTPS
protocol on port 443.
• The firewall allows outbound communication from the
StatusScope Remote Monitoring Service to aws-iot. At
a minimum, outbound communication must be enabled
for a191fe2mx36xf8-ats.iot.us-east-1.amazonaws.com.

(Optional) A network or IT specialist is available to install

   
the security software while the FSE is present.
A network printer, or a dedicated printer, and the required   —
print drivers are available.

Software Requirements
Refer to Software Requirements on page 36.

Requirement Complete N/A

All SCIEX software that will be installed by the FSE, has
   
been purchased and is available at the time of installation.

Note: Software can be downloaded from the SCIEX

website, or a software installation DVD can be
purchased. Contact the sales representative to purchase
software DVDs, if required.

Environmental Requirements
Refer to Environmental Requirements on page 36.

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 Site Planning Checklist

Requirement Complete N/A

An ambient temperature of 15 °C to 30 °C (59 °F to 86 °F)   —
is maintained.
Over time, the temperature remains within a range of 4 °C
(7.2 °F), with the rate of the change in temperature not
exceeding 2 °C (3.6 °F) per hour. Ambient temperature
fluctuations exceeding the limits might result in mass shifts
in spectra.

Note: Peripheral devices might have different operating

environment requirements. Confirm the operating
environment requirements with the manufacturer of each
peripheral device that will be used with the system.

Relative humidity is 20% to 80%, non-condensing.   —

Air conditioning provides a minimum of 6,500 Btu/hr for   —
the mass spectrometer and roughing pump only.
(Optional) A customer-supplied soft wall is installed behind
   
the mass spectrometer to reduce noise.

BioSafety Requirements
Refer to BioSafety Requirements on page 37.
Requirement Complete N/A
The site is not designated as BioSafety Level 3 (BSL-3)   —
or BioSafety Level 4 (BSL-4).

Solutions and Equipment Requirements

Refer to Solutions and Equipment Requirements on page 37.

Requirement Complete N/A

All of the required solutions and bottles are available.   —
All of the required LC equipment and supplies are   —
available.
All of the materials required for Customer Familiarization
   
are available.

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Site Planning Checklist

Product Familiarization
Requirement Complete N/A
Internet access is available. SCIEX recommends that the   —
acquisition computer be used, but alternatively, another
computer or an Android or iOS mobile device can be used.
An account has been created on sciex.com, and the
   
account has been validated, following the instructions in
the e-mail from SCIEX.

Note: Validation automatically registers the mass

spectrometer for support, grants access to productivity
tools, and enrolls the customer in the appropriate courses
at SCIEX University.

The Introduction to LC-MS/MS Operation Series for SCIEX

   
Triple Quadrupole and QTRAP Systems eLearning has
been completed. A certificate of completion is available.

Note: If the prelearning is not completed, then a

demonstration will be substituted for the hands-on portion
of the Customer Familiarization. If the customer waives
Customer Familiarization, then the FSE only provides
training on SCIEX Now resources.

System documentation has been obtained and reviewed.   —

The documentation can be downloaded from
sciex.com/customer-documents.
(Optional) A qualified maintenance person (QMP), who
   
is suitably aware of the electrical and chemical risks
associated with servicing laboratory equipment, is
available for review of the service procedures with the
FSE.

Additional Site Planning

Requirement Complete N/A
Site planning for optional peripheral devices and software
   
is completed, as required.

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 Site Planning Checklist

Comments and Exceptions

Signoff
Site planner contact
name
I acknowledge that all of the installation requirements, as specified in this document,  
have been met.
Site planner signature Completion date
(yyyy-mm-dd)

FSE name Return date

(yyyy-mm-dd)
FSE e-mail

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Site Requirements A

Site Layout Requirements

Return to checklist
Before delivery, do the following:
• Measure the clearances of all doors, external and internal, to make sure that the system can
be moved to its final location.
• Measure the clearance of the elevator and make sure that the elevator is available at the time
of delivery.
• Measure the installation location to make sure that it will accommodate the equipment.

Laboratory Layout and Site Clearances

Make sure that the site meets the required building clearances for installation and service access.
Refer to the following figure.

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 Site Requirements

Figure A-1 Laboratory Layout

Item Description
1 Distance to the wall. For fixed bench configurations, a minimum of 1 m (40 inches)
is required. For moveable bench configurations, a minimum of 0.3 m (12 inches)
is required.

WARNING! Electrical Shock Hazard. Make sure that the

system can be disconnected from the mains supply outlet
in an emergency. Do not block the mains supply outlet.

2 Acquisition computer
3 Distance to the computer, up to approximately 3 m (120 inches), allow for slack
4 Optional LC components
5 Mass spectrometer (front), 79 cm (32 inches) × 79 cm (32 inches) (length,
including the ion source)

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Site Requirements

Note: If the bench is moveable, then

• Make sure that the bench is fixed during normal use.
• Make sure that the mass spectrometer can be moved to a location with 1 m (40 inches) of
clearance on all sides, for service access.

Weights and Dimension

Refer to the following tables for weights and dimensions. Make sure that the system can be moved
to the installation site. Make sure that the installation site can accommodate the equipment
dimensions, weight, and associated clearance.
The mass spectrometer has been designed to support 77 kg (169 lbs).

Table A-1 Mass Spectrometer and Bench

Equipment Height Width Length Weight

Crate 85 cm 112 cm 100 cm 88 kg (194 lbs)
(34 inches) (44 inches) (39 inches)
Mass spectrometer 59 cm 79 cm 79 cm 130 kg
(24 inches) (32 inches) (32 inches) (285 lbs)
Roughing pump 22 cm 30 cm 42 cm 34 kg (75 lbs)
(9 inches) (12 inches) (17 inches)
Mass spectrometer bench 77 cm 92 cm 79 cm 85 kg (187 lbs)
(optional) (31 inches) (36 inches) (32 inches)
Mass spectrometer bench with 76 cm 92 cm 79 cm 120 kg
gas generator (optional) (30 inches) (36 inches) (32 inches) (264 lbs)
Accessories crate 129.5 cm 104 cm 132 cm 145 kg
(51 inches) (41 inches) (52 inches) (320 lbs)

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 Site Requirements

Table A-2 Acquisition Computer

Equipment Height Width Length Weight

Acquisition computer 41.8 cm 17.7 cm 51.8 cm 21.7 kg
(16.5 inches) (6.95 inches) (20.4 inches) (48 lbs)
Monitor, with stand 35.3 cm 48.7 cm 16.6 cm 4.72 kg
(13.91 inches) (19.19 inches) (6.54 inches) (10.41 lbs)
to 47.2 cm
(18.58 inches)
Shipping weight The acquisition computer and monitor are included in the
Accessories crate.

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Site Requirements

Electrical Requirements
Return to checklist

WARNING! Electrical Shock Hazard. Use only qualified personnel for the
installation of all of the electrical supplies and fixtures, and make sure that
all of the installations adhere to local regulations and safety standards.

The power consumption for the mass spectrometer and the roughing pump is 2,200 VA (50 Hz or
60 Hz) at 230 VAC. An external line transformer is not required for the mass spectrometer, optional
mass spectrometer bench, or roughing pump.
If the voltage changes more than 10% in 24 hours, then use a power conditioner. High or low
voltages can adversely affect the electronic components of the equipment.

Note: Additional components, such as a gas generator or LC system, require additional capacity.

Mains Supply Connections

WARNING! Electrical Shock Hazard. Make sure that the system can be
disconnected from the mains supply outlet in an emergency. Do not block
the mains supply outlet.

Provide a minimum of three branch circuits. Refer to Figure A-2.

• One branch circuit for the mass spectrometer.
• One branch circuit for the roughing pump.
• One branch circuit for the acquisition computer, monitor, and printer. This branch circuit can
also be used for options such as:
• Mass spectrometer bench
• (Optional) One branch circuit for a standalone gas generator with a compressor. Contact the
manufacturer of the gas generator for more information. The optional mass spectrometer bench
can also be connected to this branch circuit.

For electrical requirements for SCIEX-supplied components, refer to System Electrical Specifications
on page 28. For requirements for other components, such as the optional LC system, contact the
manufacturer.

Note: Use receptacles that comply with local standards. Receptacles shown in the following
figure are representations only.

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Figure A-2 Branch Circuit Configuration

Item Description
1 Branch circuit for the mass spectrometer. One outlet is required. The outlet
must be within 1.6 m (63 inches) of the mass spectrometer.
2 Branch circuit for the roughing pump. One outlet is required. The outlet must
be within 1.6 m (63 inches) of the roughing pump.
3 Additional branch circuit for the acquisition computer, monitor, and printer,
as well as any options. The optional mass spectrometer bench can be
connected to this branch circuit.
4 (Optional) One branch circuit, with one or more outlets, for a standalone gas
generator with a compressor. Contact the manufacturer of the gas generator
for more information. The optional mass spectrometer bench can be connected
to this branch circuit.

International Requirements

• For installations outside of North America, use locally approved standard connections and
cables.

Note: Refer to the Parts and Equipment Guide for recommended mains supply cables.

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Table A-3 Socket Types

Region Mains Supply Socket Type

Australia/New Zealand AS/NZS 3112
Central Europe CEE 7/7
China GB-2009
India IS1293
Italy CEI 23-50 or CEE 7/7
Japan L6/20P
North America CSA/NEMA 6-15
South Korea KSC8305
Switzerland SEV1011
United Kingdom/Ireland BS1363-1

North American Requirements

• Branch circuits for the mass spectrometer and roughing pump must be 15 A, 200 VAC to
240 VAC (typical 208 VAC), 50 Hz or 60 Hz. Refer to Figure A-2, items 1 and 2. Receptacles
are equipped with CSA/NEMA 6-15R straight blade receptacles. Refer to Figure A-3.
• The branch circuit for the acquisition computer and monitor can be 15 A, 120 VAC. Refer to
Figure A-2, item 4 .
• (Optional) Branch circuits and receptacles for the gas generator must meet the requirements
in the documentation supplied by the manufacturer.

Figure A-3 Duplex Receptacle and Plug

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Mains Supply Fluctuations

In areas where the mains supply is subject to voltage fluctuations exceeding ±10% of the nominal
value (200 VAC to 240 VAC), a power conditioner is required. High or low voltages can adversely
affect the electronic components of the system. Refer to Uninterruptible Power Supply or Power
Conditioner on page 27.

Note: Peripheral devices might have different mains supply fluctuation limits. Confirm the mains
supply fluctuation limit with the manufacturer of each peripheral device that will be used with the
mass spectrometer.

Protective Earth Conductor

WARNING! Electrical Shock Hazard. Do not intentionally interrupt the

protective earth conductor. Any interruption of the protective earth conductor
creates an electrical shock hazard.

The mains supply must include a correctly installed protective earth conductor. The protective
earth conductor must be installed or inspected by a qualified electrician before the system is
connected.

Uninterruptible Power Supply or Power Conditioner

Use a pure sine-wave uninterruptible power supply (UPS) or power conditioner to allow a safe
shutdown of the mass spectrometer, computer, monitor, and roughing pump during power outages.

Note: The FSE will install optional UPS equipment purchased from SCIEX. The customer is
responsible for installing any customer-supplied UPS equipment.

Table A-4 UPS and Power Conditioner Requirements

Specification Value
Output voltage 200 VAC to 240 VAC true online double-conversion
Frequency 50 Hz or 60 Hz
Waveform Pure sine-wave
Minimum peak current 3 × nominal current
Output voltage distortion < 3%

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Site Requirements

Table A-4 UPS and Power Conditioner Requirements (continued)

Specification Value
Output protection Circuit breaker
Minimum power requirement 4,000 VA
(mass spectrometer and
roughing pump)

System Electrical Specifications

The following tables contain the electrical specifications for the mass spectrometer, roughing pump,
computer, and monitor.

Note: Specifications are subject to change without notice.

Table A-5 Mass Spectrometer

Specification Value
Nominal input voltage 200 VAC to 240 VAC
Frequency 50 Hz or 60 Hz
Maximum current limit 10 A
Maximum power limit 2,400 VA

Table A-6 Roughing Pump

Specification Value
Nominal input voltage 200 VAC to 240 VAC
Frequency 50 Hz or 60 Hz
Maximum current limit 4.2 A
Maximum power limit 1,008 VA

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Table A-7 Computer

Specification Value
Computer
Nominal input voltage 100 VAC to 240 VAC
Frequency 50 Hz or 60 Hz
Maximum current limit 8.0 A / 6.0 A
Maximum power limit 1,920 VA / 1,440 VA
Monitor
Nominal input voltage 100 VAC to 240 VAC
Frequency 50 Hz or 60 Hz ±3 Hz
Maximum current limit 1.5 A (typical)
Maximum power limit 360 VA

Table A-8 System Bench

Specification Value
Nominal input voltage 200 VAC to 240 VAC
Frequency 50 Hz or 60 Hz
Maximum current limit 1A
Maximum power limit 240 VA

Gas Supply Requirements

Return to checklist

WARNING! Asphyxiation Hazard. Use only qualified personnel for the installation
of all gas supplies and connections, and make sure that all installations adhere to
local regulations and safety standards. Nitrogen gas can be an asphyxiant if released
in environments with insufficient ventilation.

CAUTION: Potential System Damage. Regulate each supply separately at the mass
spectrometer to prevent over-pressure damage to the mass spectrometer.

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Site Requirements

CAUTION: Potential System Damage. Do not use oil-filled regulators or gauges, and make
sure that tubing is oil-free and cleaned to medical grade standards. Oil can cause
contamination of, and damage to, the system. We recommend the use of regulators and
gauges that are stated not to be oil-filled.

CAUTION: Potential System Contamination. Use compression fittings for gas line
connections. Do not use liquid pipe sealant. If threaded fittings must be used, then Teflon
tape can be used to seal the threads. Do not use soldered fittings unless the tubing is
thoroughly cleaned afterwards.

Figure A-4 Recommended Gas Connections

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Item Gas Description Pressure Flow Rate Tubing

(max)
1 Gas for the Ultra-high 55 psi 15 L/min 1/4 inch
Curtain Gas purity (UHP) (3.79 bar) (0.64 cm)
Interface/bath N2 (99.999%) minimum to
gas/CAD gas only 60 psi
(4.14 bar)
maximum
2 Gas 1/Gas Zero-grade air, 100 psi 22 L/min 1/4 inch
2/bath or UHP N2 (6.89 bar) (0.64 cm)
gas/sheath gas minimum to
105psi(7.25bar)
maximum
3 Source Clean, dry, 55 psi 25 L/min 1/4 inch
exhaust oil-free air, or (3.79 bar) (0.64 cm)
UHP N2 minimum to
60 psi
(4.14 bar)
maximum

Note: We recommend the use of zero-grade air instead of nitrogen for Gas 1/Gas 2, because it
improves sensitivity and signal-to-noise under some conditions.

Note: Gas quality, flows, and pressures must meet the specified requirements, or a
SCIEX-approved gas generator must be used. Refer to Figure A-4.

Note: Under normal conditions, bath gas is taken from the same supply as the Gas 1 and Gas 2
flows.

Note: The air input fitting on the back panel is a quick-connect. Refer to Figure A-5. The nitrogen
input fitting is a 1/4-inch Swagelok connection. Refer to Figure A-6. All connections to the
laboratory supply are Swagelok connections. Refer to Figure A-6.

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Site Requirements

Figure A-5 Quick-Connect Fitting

Figure A-6 Swagelok Connection and Tubing

Optional Gas Generators

Gas generators are available from SCIEX. Contact a sales representative for more information.

Note: The gas generators recommended by SCIEX are designed to optimize the long-term
performance of the system. The use of an additional inline filter is not recommended.

Refer to the documentation supplied by the manufacturer for specifications, and for the number
and type of outlets required.

Ventilation and Waste Collection Requirements

Return to checklist

WARNING! Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard.

Make sure that the source exhaust system is connected and functioning,
and that good general laboratory ventilation is provided. Adequate laboratory
ventilation is required to control solvent and sample emissions, and to
provide for the safe operation of the system.

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WARNING! Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard.

Use only qualified personnel for the installation of plumbing and ventilation
fixtures, and make sure that all installations follow local bylaws and
regulations.

CAUTION: Potential System Damage. Do not connect the source exhaust hose to the vent.
The connection must be made by a qualified FSE.

• Make sure that the ventilation of the laboratory environment in which the system will be used
complies with local regulations, and that the air exchange rate is appropriate for the work
performed.

Note: A minimum of 10 air exchanges/hour is required for laboratory applications using toxic
agents.

• Provide a ventilation system with a total negative flow rate capacity of 283 L/min (10 cfm). Refer
to Figure A-7, item 1.
• Provide a smooth fitting for the roughing pump, with an outside diameter (o.d.) of 3.2 cm
(1.25 inches). The vent must be within 1.5 m (60 inches) of the exhaust port on the roughing
pump, and at least 1 m (40 inches) above the floor. Refer to Figure A-7.
• Provide a fitting for the source exhaust drain bottle with an o.d. of 2.5 cm (1 inch). The vent
must be within 1.5 m (60 inches) of the source exhaust drain bottle. Refer to Figure A-7.

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Site Requirements

Figure A-7 Vent Connections

Item Description
1 Vent.
2 Source exhaust hose: 2.5 cm (1 inch) inside diameter (i.d.)
3 Roughing pump exhaust hose: 3.2 cm (1.25 inches) i.d.
4 Source exhaust drain bottle. Make sure that the bottle is secured at all times
to prevent spills.
5 Source exhaust drain tubing: 1.6 cm (0.625 inch) i.d.

Note: Source exhaust hose connections at the drain bottle, mass spectrometer, and the lab vent
are secured with hose clamps.

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Computer, Network, and Software Requirements

Return to checklist

Acquisition Computer Requirements

The acquisition computer and monitor are provided with the mass spectrometer. The acquisition
computer controls the mass spectrometer, and should be used exclusively for data acquisition.
• Provide a table for the acquisition computer and monitor, within 3 m (120 inches) of the mass
spectrometer.
• Provide a network computer name and a domain password that comply with these guidelines:

Note: Configure the network before installing the acquisition software.

• The computer name does not have any spaces.

• The password for the computer is not blank.

CAUTION: Potential System Damage. Other than security software, do not install any
additional software on the acquisition computer. Changes to the configured software
could void the system warranty and cause the system to stop operating.

• If security software is required, then make sure that an IT specialist is available to install the
anti-virus, anti-malware, or backup software while the FSE is present.
For the latest guidance on cybersecurity for SCIEX products, visit sciex.com/productsecurity.

Printer Requirements
If necessary, establish a connection to a network or local printer to print from the control software.

Note: To use a network printer, connect the acquisition computer to the company network.

• Make sure that a printer and its associated print drivers are available before the scheduled
installation.

LAN Connection
To connect the acquisition computer to the network:
• Make sure that an active, tested LAN connection is in place before the scheduled installation
date.

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Site Requirements

• Provide network credentials for the acquisition computer that comply with the guidelines provided
for the computer name and password, as specified previously.

Note: Do not change the network computer name after the software has been installed.

• Make sure that an IT specialist is available to connect the computer to the LAN while the FSE
is present.

Software Requirements
Return to checklist
SCIEX software purchased and intended to be installed by the FSE must be available at the time
of installation. Software can be obtained in one of the following ways:
• Download the software from sciex.com/software-support/software-downloads.

Note: Internet access is required to download the software. We recommend that all of the
software be downloaded in advance of the scheduled installation to expedite the installation.

• Purchase the software installation DVD. For more information, contact the SCIEX sales
representative. The DVD must be purchased in advance and it must be available during the
hardware installation.

Environmental Requirements
Return to checklist
• An ambient temperature of 15 °C to 30 °C (59 °F to 86 °F)
Over time, the temperature must remain within a range of 4 °C (7.2 °F), with the rate of the
change in temperature not exceeding 2 °C (3.6 °F) per hour. Ambient temperature fluctuations
exceeding the limits might result in mass shifts in spectra.
• Relative humidity from 20% to 80%, non-condensing
• Cooling and venting for the mass spectrometer and roughing pump: 6,500 Btu/hr

CAUTION: Potential System Damage. Do not install the roughing pump in an enclosed,
unventilated area. Installing the roughing pump in an unventilated area will cause it to
shut down due to overheating, and can cause severe damage to the mass spectrometer.

Note: Operation of the system at altitudes higher than 2,000 m (6,400 feet) above sea level might
impact system operation.

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Note: Peripheral devices might have different operating environment requirements. Confirm the
operating environment requirements with the manufacturer of each peripheral device that will be
used with the system.

Sound Pressure Level

Sound Pressure Value (dBA)
Average sound pressure level for the mass spectrometer 65 to 70

Many regulatory jurisdictions have noise standards and threshold levels to protect workers from
noise induced hearing loss. These standards require that the noise level to which workers are
exposed is less than an 8 hour time weighted average of 85 dB.

Vibration
• The packaged system was tested to confirm that the effects of transportation do not affect
system performance upon arrival at the customer destination.
• The system is designed to operate in any laboratory environment that accommodates precision
analytical instrumentation.

BioSafety Requirements
The site must not be designated BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4). SCIEX
does not install, service, or repair SCIEX systems in areas designated BSL-3 or BSL-4.

Solutions and Equipment Requirements

Return to checklist

WARNING! Toxic Chemical Hazard. Refer to the chemical product Safety Data
Sheets and follow all of the recommended safety procedures when handling, storing,
and disposing of chemicals. For health and safety precautions, refer to the System
User Guide.

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Site Requirements

Customer Familiarization
Customer-Supplied Materials

Description Size Quantity

Glass bottle, rinsed thoroughly to meet 100 mL 1
standards of use with mass spectrometry
Glass bottle, rinsed thoroughly to meet 1L 2
standards of use with mass spectrometry
Beaker, rinsed thoroughly to meet 250 mL 2
standards of use with mass spectrometry
Pre-blended LC-MS-grade methanol with 2.5 L 1
1
0.1% formic acid
Pre-blended LC-MS-grade water with 0.1% 2.5 L 1
1
formic acid

Customer-Supplied Lab Equipment

Description Size Quantity

Adjustable micropipettes 100 µL and 1 mL 2
recommended
Boxes of pipette tips 100 µL and 1 mL 2
recommended
Measuring cylinder 100 mL 2
Vortex mixer (optional) N/A 1
Glass transfer pipette N/A 1
PEEK tubing cutter N/A 1

Mass Spectrometer Operation

In addition to the materials specified in Customer Familiarization on page 38, the following are
required:
• Appropriate personal protective equipment, including powder-free gloves and safety glasses

Note: Nitrile or neoprene gloves are recommended

1 If pre-blended solvent is not available, then use the procedure in the Customer Familiarization Checklist to prepare the solvent.

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• LC-MS-grade acetonitrile (2 L), stored in glass bottles

• LC-MS-grade ammonium acetate (100 mg), stored in a dessicator
• LC-MS-grade isopropanol (2 L), stored in glass bottles
• LC-MS-grade methanol (2 L)
• Pipettors (20 µL, 100 µL or 200 µL, 1 mL) and tips
• LC system, including the solvent bottles, waste collection container, and required tubing and
cutter, unless ordered through SCIEX.
For requirements and specifications for the LC system, contact the manufacturer.
• Mass spectrometer bench, unless ordered through SCIEX. Refer to Weights and Dimension
on page 22.
• Table for the computer and monitor
• Printer
• Refrigeration for the Standards Chemical Kit with High/Low Concentration PPGs, provided by
SCIEX
• (Recommended) A secondary containment tray to be installed beneath the roughing pumps,
to capture potential chemical spills

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Equipment Safety Categories B
Description Category
Equipment pollution degree Pollution Degree 2
Mains supply transient overvoltage Overvoltage Category II

DANGER! Explosion Hazard. Do not operate the system in an environment containing

explosive gases. The system is not designed for operation in an explosive
environment.

Note: Environments with a Pollution Degree 2 rating include laboratories and sales and commercial
areas.

For more information, refer to the International Electrotechnical Commission standards IEC 61010-1
and IEC 60364.

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Glossary of Symbols C
Note: Not all of the symbols in the following table are applicable to every instrument.

Symbol Description
Australian Regulatory Compliance Mark. Indicates that the product complies
with Australian Communications Media Authority (ACMA) EMC Requirements.

Alternating current

A Amperes (current)
Asphyxiation Hazard

Authorized representative in the European community

Biohazard

CE Marking of Conformity

cCSAus mark. Indicates electrical safety certification for Canada and USA.

Catalogue number

Caution. Consult the instructions for information about a possible hazard.

Note: In SCIEX documentation, this symbol identifies a personal injury

hazard.

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Glossary of Symbols

Symbol Description
China RoHS Caution Label. The electronic information product contains certain
toxic or hazardous substances. The center number is the Environmentally
Friendly Use Period (EFUP) date, and indicates the number of calendar years
the product can be in operation. Upon the expiration of the EFUP, the product
must be immediately recycled. The circling arrows indicate the product is
recyclable. The date code on the label or product indicates the date of
manufacture.

China RoHS logo. The device does not contain toxic and hazardous
substances or elements above the maximum concentration values and it is
an environmentally-friendly product that can be recycled and reused.
Consult instructions for use.

Crushing Hazard

cTUVus mark for TUV Rheinland of North America.

Data Matrix symbol that can be scanned by a barcode reader to obtain a

unique device identifier (UDI).

Environmental Hazard

Ethernet connection

Explosion Hazard

Eye Injury Hazard

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 Glossary of Symbols

Symbol Description
Fire Hazard

Flammable Chemical Hazard

Fragile

Fuse

Hz Hertz
International safety symbol "Caution, risk of electric shock" (ISO 3864), also
known as High Voltage symbol
If the main cover must be removed, then contact a SCIEX representative to
prevent electric shock.
Hot Surface Hazard

In Vitro Diagnostic Device

Ionizing Radiation Hazard

Keep dry.
Do not expose to rain.
Relative humidity must not exceed 99%.
Keep upright.

Lacerate/Sever Hazard

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Glossary of Symbols

Symbol Description
Laser Radiation Hazard

Lifting Hazard

Magnetic Hazard

Manufacturer

Moving Parts Hazard

Pacemaker Hazard. No access to people with pacemakers.

Pinching Hazard

Pressurized Gas Hazard

Protective Earth (ground)

Puncture Hazard

Reactive Chemical Hazard

Serial number

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Symbol Description
Toxic Chemical Hazard

Transport and store the system within 66 kPa to 103 kPa.

Transport and store the system within 75 kPa to 101 kPa.

Transport and store the system within the specified minimum (min) and
maximum (max) levels of relative humidity, non-condensing.

Transport and store the system within –30 °C to +45 °C.

Transport and store the system within –30 °C to +60 °C.

USB 2.0 connection

USB 3.0 connection

Ultraviolet Radiation Hazard

United Kingdom Conformity Assessment Mark

VA Volt Ampere (power)

V Volts (voltage)
WEEE. Do not dispose of equipment as unsorted municipal waste.
Environmental Hazard

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Glossary of Symbols

Symbol Description
W Watts
yyyy-mm-dd
Date of manufacture

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Contact Us

Customer Training
• In North America: [email protected]
• In Europe: [email protected]
• Outside the EU and North America, visit sciex.com/education for contact information.

Online Learning Center

• SCIEX University

SCIEX Support
SCIEX and its representatives maintain a staff of fully-trained service and technical specialists
located throughout the world. They can answer questions about the system or any technical issues
that might arise. For more information, visit the SCIEX website at sciex.com or contact us in one
of the following ways:
• sciex.com/contact-us
• sciex.com/request-support

CyberSecurity
For the latest guidance on cybersecurity for SCIEX products, visit sciex.com/productsecurity.

Documentation
This version of the document supercedes all previous versions of this document.
To view this document electronically, Adobe Acrobat Reader is required. To download the latest
version, go to https://get.adobe.com/reader.

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Contact Us

To find software product documentation, refer to the release notes or software installation guide
that comes with the software.
To find hardware product documentation, refer to the Customer Reference DVD that comes with
the system or component.
The latest versions of the documentation are available on the SCIEX website, at
sciex.com/customer-documents.

Note: To request a free, printed version of this document, contact sciex.com/contact-us.

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