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Application Note Nitrate (P260) Method: Trace2o LTD Rev1.0

This document provides instructions for using an HT1000 Photometer to detect nitrate levels in water samples. Key steps include zeroing the instrument with deionized water, adding reagents to the water sample, allowing it to react for 10 minutes, and taking a measurement to determine the nitrate concentration in mg/L. The method can detect nitrate from 0.08 to 1 mg/L, with a tolerance of ±0.005 mg/L. Notes provide additional guidance on handling samples and potential interferences.

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Танюшка Кузнецова
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132 views4 pages

Application Note Nitrate (P260) Method: Trace2o LTD Rev1.0

This document provides instructions for using an HT1000 Photometer to detect nitrate levels in water samples. Key steps include zeroing the instrument with deionized water, adding reagents to the water sample, allowing it to react for 10 minutes, and taking a measurement to determine the nitrate concentration in mg/L. The method can detect nitrate from 0.08 to 1 mg/L, with a tolerance of ±0.005 mg/L. Notes provide additional guidance on handling samples and potential interferences.

Uploaded by

Танюшка Кузнецова
Copyright
© © All Rights Reserved
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®

HT1000

APPLICATION NOTE T2O-AN-P260

NITRATE (P260) METHOD


The following application note explains the procedure for the detection of Nitrate (P260) using the
HT1000 Photometer.

Equipment:

 HT1000 Photometer
 Nitrate test tube
 Nitrate test powder
 Nitrate Test tablet
 10ml vial
 Stirring rod
 Nitrite LR tablet

Safety:

 Consult the safety data sheet for all of the reagents before use. Even if you have used
HydroTest reagents before, the formulation may have changed.

Getting started:

 Switch the unit on using the power key.

 Select the Nitrate method by depressing the cursor keys until ‘Nitrate T ’ is displayed or use
the shortcut by pressing & holding shift followed by 260

Release shift and press enter.

Trace2o Ltd Rev1.0


Blank analysis:

 Ensure that the 10ml vial is clean.


 Fill the vial with 10ml of the water sample.
 Fit the cap and tighten.
 Place the vial in the sample chamber, making sure that the arrow marks on the instrument
and vial are aligned.
 Press the zero key

 Wait until the following is displayed


Zero accepted
Prepare Test
Press TEST

Sample preparation:

 Fill the nitrate test tube with 20ml of the water sample.
 Add 1 level spoon of Nitrate Test powder
 Close the tube tightly with the cap and swirl vigorously for one minute
 Add one Nitrate Test tablet straight from the foil to the water sample.
 Close the tube tightly with the cap and swirl vigorously for one minute
 Stand the tube upright, allow contents to settle, then gently invert three to four times.
 Allow to stand for a further 2 minutes, then open and wipe carefully around the rim to
remove solid particles.
 Decant 10ml of the treated solution into the 10ml vial.
 Add one Nitrite LR tablet straight from the foil to the water sample.
 Crush the tablet using a clean stirring rod, until no large pieces are visible.
 Close the vial tightly with the cap and swirl several times until the tablet is dissolved.

Analysis :

 Place the vial in the sample chamber, making sure that the marks on the instrument and vial
are aligned.
 Press the Test key

 Wait for a reaction period of 10 minutes


 After the reaction period, the measurement starts and the result is displayed in mg/l Nitrate

LOD/Tolerance
 The Lower LOD is 0.08 mg/l (80ppb), upper LOD is 1 mg/l (1000ppb).
 Tolerance: ± 0.005mg/l.

Trace2o Ltd Rev1.0


Notes

 For best results, rinse vials thoroughly between with deionised water. Ensure that the
outside of the vials are clean, dry and free from fingerprints. Always handle vials by the lid
where possible.
 The tablets should be added direct from the foil avoiding contact with hands or surfaces.
 Nitrite present in the sample will also react, leading to a higher result. To correct, carry out a
nitrite test (P270) on the same sample and subtract the result.
 Nitrate concentrations above 1 mg/L can be diluted up to 100x, with the result multiplied up
to compensate.
 Interferences may occur from presence of the following ions: Antimony (III), Iron (III), Lead,
Mercury (I), Silver, Chloroplatinate, Metavanadate, Bismuth.
 Copper (II) ions may artificially lower test results.

Trace2o Ltd Rev1.0


Trace2o Ltd Rev1.0

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