CY009

English
CYANSmart
Application Sheets

For a clear and precise diagnose

ISO 13485-2016
www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
Layout 20181024 www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 DIRECTIONS FOR USE

AUTOMATION

CYANSmart

CLINICAL CHEMISTRY

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium

www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 Page Test Program Name
1 α-Amylase AAMY
2 Acid Phosphatase Total ACPt
3 Acid Phosphatase (Non) Prostatic ACP(n)p
4 Albumin ALB
5 Alkaline Phosphatase (ALP) Liquid ALPL
6 + 7-8 Bilirubin Direct + Scheme BILD
9 + 10-11 Bilirubin Total + Scheme BILT
12 Calcium CaAR
13 Chloride CL
14 Cholesterol Lyophilized CHOL
15 Cholesterol Liquid (new) CHOLLn
16 + 17 HDL Cholesterol Direct + Scheme HDLd
18 + 19 LDL Cholesterol Direct + Scheme LDLd
20 Creatine Kinase MB Liquid CKMBL
21 Creatine Kinase NAC Liquid CKNACL
22 Creatinine CRE
23 G6-PDH G6PDH
24 Glucose Lyophilized GLUC
25 Glucose Liquid GLUCLn
26 GT Liquid GGTL
27 GOT (AST) Liquid GOTL
28 GPT (ALT) Liquid GPTL
29 HbA1c HbA1c
30 Hemoglobin HGB
31 Iron IRON
32 LDH Liquid LDHL
33 Lipase LIPASE
34 Magnesium MgXB
35 Phosphorus PHOSPH
36 Potassium POT
37 Sodium SOD
38 Total Protein TP
39 Total Protein in Urine and CSF TPU
40 Triglycerides Lyphilized TRIG
41 Triglycerides Liquid (new) TRIGLn
42 Urea Lyophilized UREA
43 Urea Liquid UREAL
44 Urea Liquid UREALn
45 Uric Acid Liquid UAL
46 LIN340 LIN340
47 LIN510 LIN510

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium

www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

REF HBE03 α-Amylase

VOL 20 x 2 mL
Standard -
CNPG3. Colorimetric. Kinetic

REAGENT PREPARATION AND STABILITY

The Amylase reagent is ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8°C.
After opening the reagent is stable for 60 days when properly capped immediately after each opening and stored at 2-8°C.The reagent should be
a clear solution. If turbidity or precipitation has occurred or if blank absorbance at 405 nm ≥ 0,40, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor. Note 2 Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
- Serum or plasma, remove from cells as soon as possible. It is recommended to use heparin as anticoagulant.
- Urine, adjust to pH approximately 7,0 prior to storage.
- Stability: 1 month at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For BlankNote 1 1,0 mL Reagent
For Sample/(Calibrator)Note 2,4 20 µL Sample/(Calibrator) + 1,0 mL Reagent
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working solution to
the sample/calibrator. Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: AAMY Blank Low: 0,0000Note 1
Program Method: Kinetic Blank High: 0,4000Note 1
Main Filter: 405 nm Normal Low: 28,0000 U/L
Sub Filter: None nm Normal High: 90,0000Note 4 U/L
Program Unit: U/L Num of STD: 0Note 2
Aspiration volume: 0800 µL CONC: 0,0000Note 2 U/L
Delay Time: 120 sec Factor: 3954,0000Note 2,4
Test time: 090 sec Control N min: Enter valueNote 3 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 U/L
Linearity Min: 0,2439 U/L Control P min: Enter valueNote 3 U/L
Linearity Max: 2200,0000 U/L Control P max: Enter valueNote 3 U/L
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,2439 U/L (detection limit) to 2200 U/L (linearity limit). If the obtained results are greater than 2200 U/L, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD (1) and
CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum or plasma samples. For urine, please check the insert for the values and sample preparation. For urine samples; use
10 µl of sample, Normal High: 450 and Factor: 7908.

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Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
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 CY009 CYANSmart
Application sheet

REF HBE01 Acid Phosphatase Total

VOL 18 x 2 mL
Standard - α-Naphtyl phosphate.
Colorimetric. Kinetic. Hillman Method

REAGENT PREPARATION AND STABILITY

R3 and R4 are ready to use.
Dissolve one tablet R2 substrate in 2mL of R1 buffer. Cap and mix gently to dissolve the contents. The stability of working reagent is 2 days at 2-8
°C or 6 hours at room temperature.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
Do not use the tablets if they appear to be broken. The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank
absorbance at 405 nm ≥ 0,44, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration.Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls Specific
(HBC01-S, HBC02-S). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Clear serum, separated from the clot as soon as possible. Do not use plasma or hemolytic serum. Acid phosphatase is extremely labile, stabilize
by adding 50 μL of acetic acid (R4) per mL of the sample. Stability: 7 days at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For BlankNote 1 1,00 mL Working reagent (R1 + R2)
For Sample/(Calibrator)Note 2 100 µL Sample/(Calibrator) + 1,00 mL Working reagent (R1 + R2)

Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working solution to
the sample/calibrator. Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: ACPt Blank Low: 0,0000Note 1
Program Method: Kinetic Blank High: 0,4400Note 1
Main Filter: 405 nm Normal Low: 0,1300 U/L
Sub Filter: None nm Normal High: 5,4000 U/L
Program Unit: U/L Num of STD: 0Note 2
Aspiration volume: 0800 µL CONC: 0,0000Note 2 U/L
Delay Time: 300 sec Factor: 750,0000Note 2
Test time: 090 sec Control N min: Enter valueNote 3 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 U/L
Linearity Min: 0,0000 U/L Control P min: Enter valueNote 3 U/L
Linearity Max: 150,0000 U/L Control P max: Enter valueNote 3 U/L
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0 U/L (detection limit) to 150 U/L (linearity limit). If the obtained results are greater than 150 U/L, dilute the sample 1:2
with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Program NUM of
STD (1) and CONC (as mentioned on the insert provided with HBC03-S).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. To obtain a value for Prostatic Acid Phosphatase (ACPp), you can calculate the ratio: ACPp = ACPt – ACPnp.

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Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

REF HBE01 Acid Phosphatase (Non) Prostatic

VOL 18 x 2 mL
Standard - α-Naphtyl phosphate.
Colorimetric. Kinetic. Hillman Method

REAGENT PREPARATION AND STABILITY

R3 and R4 are ready to use.
Dissolve one tablet R2 substrate in 2mL of R1 buffer. Cap and mix gently to dissolve the contents. The stability of working reagent is 2 days at 2-8
°C or 6 hours at room temperature.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
Do not use the tablets if they appear to be broken. The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank
absorbance at 405 nm ≥ 0,44, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration.Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls Specific
(HBC01-S, HBC02-S).Note 3 Prepare and measure these controls the same as samples. If control values are found outside the defined range, check
the instrument, reagents and calibrator for problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not
meet the acceptable tolerances.

SAMPLES
Clear serum, separated from the clot as soon as possible. Do not use plasma or hemolytic serum. Acid phosphatase is extremely labile, stabilize
by adding 50 μL of acetic acid (R4) per mL of the sample. Stability: 7 days at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For BlankNote 1 1,00 mL Working reagent (R1 + R2) + 10 µL Reagent 3
For Sample/(Calibrator)Note 2 100 µL Sample/(Calibrator) + 1,00 mL Working reagent (R1 + R2) + 10 µL Reagent 3
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working solution to
the sample/calibrator. Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: ACPnp Blank Low: 0,0000Note 1
Program Method: Kinetic Blank High: 0,4400Note 1
Main Filter: 405 nm Normal Low: 0,1300 U/L
Sub Filter: None nm Normal High: 5,4000 U/L
Program Unit: U/L Num of STD: 0Note 2
Aspiration volume: 0800 µL CONC: 0,0000Note 2 U/L
Delay Time: 300 sec Factor: 750,0000Note 2
Test time: 090 sec Control N min: Enter valueNote 3 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 U/L
Linearity Min: 0,0000 U/L Control P min: Enter valueNote 3 U/L
Linearity Max: 150,0000 U/L Control P max: Enter valueNote 3 U/L
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0 U/L (detection limit) to 150 U/L (linearity limit). If the obtained results are greater than 150 U/L, dilute the sample 1:2
with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03-S).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. To obtain a value for Prostatic Acid Phosphatase (ACPp), you can calculate the ratio: ACPp = ACPt – ACPnp.

2021-09 (5.0) - Replaces all previous versions

3/49
Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

REF HB0010 HB0010A HB0010M Albumin

VOL 2 x 125 mL 8 x 125 mL 8 x 30 mL
Standard 1 x 5 mL 4 x 5 mL -
Bromocresol Green. Colorimetric

REAGENT PREPARATION AND STABILITY

Reagent and standard are ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-25°C.
Once open, the standard is stable up to 3 months.
Do not exceed the temperature of 25°C during storage. The reagent should be a clear, yellow-green solution. If turbidity or precipitation has
occurred or if blank absorbance at 620 nm ≥ 0,40, the reagent should be discarded.Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Note 2 Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples. If control values are found outside the defined range mentioned on the
insert of the control, check the instrument, reagents and calibrator for problems. Each laboratory should establish its own QC scheme and
corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma, free of hemolysis. Stability 1 month at 2-8°C or 1 week at 15-25°C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL Reagent
For StandardNote 2 5 µL Standard + 1 mL Reagent
For Sample 5 µL Sample + 1 mL Reagent
You can prepare several samples simultaneously. Mix and incubate for 10 minutes at room temperature. After the incubation time, aspirate and
measure the samples within 1 hour after preparation.

PROGRAM SETUP
Program Name: ALB Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,4000Note 1
Main Filter: 620 nm Normal Low: 3,5000Note 4 g/dL
Sub Filter: None nm Normal High: 5,0000Note 4 g/dL
Program Unit: g/dL Num of STD: 1Note 2
Aspiration volume: 0800 µL CONC: (value see vial)Note 2 g/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 g/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 g/dL
Linearity Min: 0,0380 g/dL Control P min: Enter valueNote 3 g/dL
Linearity Max: 5,8000 g/dL Control P max: Enter valueNote 3 g/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,038 g/dL (detection limit) to 5,8 g/dL (linearity limit). If the obtained results are greater than 5,8 g/dL, dilute the sample
1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

2021-02 (4.0) - Replaces all previous versions

4/49
Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

REF HBE12 Alkaline Phosphatase

VOL 60 + 15 mL
Standard -
IFCC. Colorimetric. Kinetic

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of R1 (buffer) with 1 volume of R2 (substrate). The stability of working reagent is 21 days at 2-8°C or 5 days at room temperature
(15-25°C).
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8°C.
Do not freeze the reagents.
The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank absorbance at 405 nm > 1,50, the reagent should be
discarded. Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor. Note 2 Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or heparinized plasma. Use non-hemolyzed serum, separated from the cloth as soon as possible. Stability: 3 days at 2-8°C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1,00 mL Working reagent (R1 + R2)
For Sample/(Calibrator)Note 2 20 µL Sample/(Calibrator) + 1,00 mL Working reagent (R1 + R2)

Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working solution to
the sample/calibrator. Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: ALPL Blank Low: 0,0000Note 1
Program Method: Kinetic Blank High: 1,5000Note 1
Main Filter: 405 nm Normal Low: 26,0000 U/L
Sub Filter: None nm Normal High: 117,0000 U/L
Program Unit: U/L Num of STD: 0Note 2
Aspiration volume: 0800 µL CONC: 0,0000Note 2 U/L
Delay Time: 060 sec Factor: 2764,0000Note 2
Test time: 090 sec Control N min: Enter valueNote 3 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 U/L
Linearity Min: 1,3070 U/L Control P min: Enter valueNote 3 U/L
Linearity Max: 1400,0000 U/L Control P max: Enter valueNote 3 U/L
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 1,307 U/L (detection limit) to 1400 U/L (linearity limit). If the obtained results are greater than 1400 U/L, dilute the sample
1:10 with saline solution, repeat the determination, and multiply the result by factor 10.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD (1) and
CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

2021-02 (4.0) - Replaces all previous versions

5/49
Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

REF HB0260 HB0020 HB0020A Bilirubin Direct

VOL 2 x 125 mL 1 x 125 mL 4 x 125 mL
Standard - - -
DMSO. Colorimetric

REAGENT PREPARATION AND STABILITY

All reagents are ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-25 °C.
The reagent should be a clear solution. If turbidity or precipitation has occurred, if color development has occurred in reagent N, or if blank
absorbance at 546 nm ≥ 0,10 the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor, only when quality controls are within the defined ranges. Otherwise, use the Biochemistry Calibrator (HBC03) for
calibration. Use the value indicated on the insert ‘with sample blank’.Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma, free of hemolysis. Store protected from direct light. Stability: 4 days at 2-8 °C and 2 months at -20 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature. Protect samples from direct sunlight.
Then, pipette into a test tube:
For Blank 750 µL Reagent D
For Sample/Cal BlankNote 2 100 µL Sample/Calibrator + 750 µL Reagent D
For Sample/CalNote 2 100 µL Sample/Calibrator + 25 µL Reagent N + 750 µL Reagent D

Thus, for every sample, you need to prepare 2 test tubes: one for measuring the sample blank (background coloration) and one for measuring
the real sample coloration. Add Reagent N only to the second tube. Add Reagent D last, mix and incubate for exactly 5 minutes at room
temperature. Aspirate the mixture in the instrument, exactly 5 minutes after addition of the Reagent D.
Use the illustrations on the next page for guidance to perform this test in a time-efficient way.

PROGRAM SETUP
Program Name: BILD Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,1000Note 1
Main Filter: 546 nm Normal Low: 0,0600 mg/dL
Sub Filter: None nm Normal High: 0,2500 mg/dL
Program Unit: mg/dL Num of STD: 0Note 2
Aspiration volume: 0550 µL CONC: 0,0000Note 2 mg/dL
Delay Time: 001 sec Factor: 19,0000Note 2
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,0600 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 20,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: SerumNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,06 mg/dL (detection limit) to 20 mg/dL (linearity limit). If the obtained results are greater than 20 mg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. The Blank method programmed is Serum! Pay attention, this determines the
calculation of the results, not the blank aspiration!
- Aspirate distilled water to adjust the instrument to zero (AD value)
- “Test Blank”  “yes” and aspirate Reagent D (blank)
- In the standard/sample menu,
a. “Aspirate Serum” = Standard/Sample Blank.
b. “Aspirate Standard/Sample” = Standard/Sample (including 25 µL of RN ).
2. Calibration by means of a Factor, only when quality controls are within the defined ranges. Otherwise, use the Biochemistry Calibrator (HBC03) for
calibration. Use the value indicated on the insert ‘with sample blank’. Program NUM of STD (1) and CONC (as mentioned on the insert provided with
HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

2021-02 (4.0) - Replaces all previous versions

6/49
Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

7/49

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium • www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

8/49

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium • www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

REF HB0270 HB0020 HB0020A Bilirubin Total

VOL 2 x 125 mL 1 x 125 mL 4 x 125 mL
Standard - - -
DMSO. Colorimetric

REAGENT PREPARATION AND STABILITY

All reagents are ready for use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-25 °C.
The reagent should be a clear solution. If turbidity or precipitation has occurred, if color development has occurred in reagent N, or if blank
absorbance at 546 nm ≥ 0,10 the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor, only when quality controls are within the defined ranges. Otherwise, use the Biochemistry Calibrator (HBC03) for
calibration. Use the value indicated on the insert ‘with sample blank’.Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples. If control values are found outside the defined range mentioned on the
insert of the control, check the instrument, reagents and calibrator for problems. Each laboratory should establish its own QC scheme and
corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma, free of hemolysis. Protect samples from direct light. Bilirubin is stable up to 4 days at 2-8 °C and 2 months at -20 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature. Protect samples from direct sunlight.
Then, pipette into a test tube:
For Blank 750 µL Reagent T
For Sample/Cal BlankNote 2 100 µL Sample/Calibrator + 750 µL Reagent T
For Sample/CalNote 2 100 µL Sample/Calibrator + 25 µL Reagent N + 750 µL Reagent T
Thus for every sample, you need to prepare 2 test tubes: one for measuring the sample blank (background coloration) and one for measuring
the real sample coloration. Add Reagent N only to the second tube. Add Reagent T last, mix and incubate for exactly 5 minutes at room temperature.
Aspirate the mixture in the instrument, exactly 5 minutes after addition of the Reagent T.
Use the illustrations on the next page for guidance to perform this test in a time-efficient way.

PROGRAM SETUP
Program Name: BILT Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,1000Note 1
Main Filter: 546 nm Normal Low: 0,2000 mg/dL
Sub Filter: None nm Normal High: 1,1000 mg/dL
Program Unit: mg/dL Num of STD: 0Note
Aspiration volume: 0550 µL CONC: 0,0000Note 2 mg/dL
Delay Time: 001 sec Factor: 12,0000Note 2
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,1000 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 20,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: SerumNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,1 mg/dL (detection limit) to 20 mg/dL (linearity limit). If the obtained results are greater than 20 mg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. The Blank method programmed is Serum! Pay attention, this
determines the calculation of the results, not the blank aspiration!
- Aspirate distilled water to adjust the instrument to zero (AD value)
- “Test Blank”  “yes” and aspirate Reagent T (blank)
- In the standard/sample menu,
a. “Aspirate Serum” = Standard/Sample Blank.
b. “Aspirate Standard/Sample” = Standard/Sample (including 25 µL of RN).
2. Calibration by means of a Factor, only when quality controls are within the defined ranges. Otherwise, use the Biochemistry Calibrator (HBC03)
for calibration. Use the value indicated on the insert ‘with sample blank’. Program NUM of STD (1) and CONC (as mentioned on the insert provided
with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

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Application sheet

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Application sheet

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 CY009 CYANSmart
Application sheet

REF HB0030 HB0030A HB0030M Calcium

VOL 2 x 125 mL 8 x 125 mL 8 x 30 mL
Standard 1 x 5 mL 4 x 5 mL -
Arsenazo III. Colorimetric. Monoreagent

REAGENT PREPARATION AND STABILITY

The reagent and standard are ready for use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 620 nm ≥ 0,80 the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma: separated from cells as rapidly as possible. Blood anticoagulants with oxalate, citrate or EDTA are not acceptable since these
chemicals will strongly chelate calcium.
Urine: collect 24 hours urine specimen in calcium free containers. The collected bottles should contain 10 mL of diluted Nitric acid (50% v/v).
Record the volume. Dilute a sample 1:2 in distilled water. Mix. Multiply results by 2 (dilution factor).
Stability of the samples: Calcium is stable 10 days at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1,0 mL Reagent
For StandardNote 2 20 µL Standard + 1,0 mL Reagent
For Sample 20 µL Sample + 1,0 mL Reagent
You can prepare several samples simultaneously. Mix and incubate for 2 minutes at 15 - 25 °C (room temperature). After the incubation time,
aspirate and measure the samples within 1 hour after preparation.

PROGRAM SETUP
Program Name: CaAR Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,8000Note 1
Main Filter: 620 nm Normal Low: 8,6000Note 4 mg/dL
Sub Filter: None nm Normal High: 10,2000Note 4 mg/dL
Program Unit: mg/dL Num of STD: 1Note 2
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,1629 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 20,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 3

MEASURING RANGE
This method is linear from 0,163 mg/dL (detection limit) to 20 mg/dL (linearity limit). If the obtained results are greater than 20 mg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum or plasma samples. For urine, please check the insert for the values and sample preparation.

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Application sheet

REF HB005 Chloride

VOL 2 x 125 mL
Standard 1 x 5 mL
Thiocyanate. Colorimetric

REAGENT PREPARATION AND STABILITY

The reagent is ready for use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank absorbance at 440-500 nm ≥ 0,15, the reagent should
be discarded. Note 1 Handle standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Note 2 Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
- Serum, plasma: Free of hemolysis and separated from cells as rapidly as possible. Anticoagulants such as oxalate or EDTA will interfere.
- Urine: Collect 24 hours urine specimen in chloride free containers. Dilute a sample 1:2 in distilled water. Mix. Multiply results by 2 (dilution factor).
- Chloride is stable 1 week at room temperature (15-25 °C), 15 days in refrigerator (2-8 °C) and 1 month frozen (-20 °C).

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1,00 mL Reagent 1
For StandardNote 2 10 µL Standard + 1,00 mL Reagent 1
For Sample 10 µL Sample + 1,00 mL Reagent 1

You can prepare several samples simultaneously. Mix and incubate for 5 minutes at 37 °C or for 5 minutes at 15 - 25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 30 minutes after preparation.

PROGRAM SETUP
Program Name: Cl Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,1500Note 1
Main Filter: 492 nm Normal Low: 95,0000Note 4 mEq/L
Sub Filter: None nm Normal High: 115,0000Note 4 mEq/L
Program Unit: mEq/L Num of STD: 1Note 2
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mEq/L
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mEq/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mEq/L
Linearity Min: 0,4540 mEq/L Control P min: Enter valueNote 3 mEq/L
Linearity Max: 190,0000 mEq/L Control P max: Enter valueNote 3 mEq/L
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,454 mEq/L (detection limit) to 190 mEq/L (linearity limit). If the obtained results are greater than 190 mEq/L, dilute the
sample 1:2 with distilled water, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum or plasma samples. For urine, please check the insert for the values and sample preparation.

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Application sheet

REF HB006 Cholesterol Lyo

VOL 2 x 125 mL
Standard 1 x 5 mL
Enzymatic-colorimetric test (CHOD-POD)

REAGENT PREPARATION AND STABILITY

Dissolve the contents of one bottle R.2 Enzymes into the contents of one bottle R.1 Buffer. Cap and mix gently to dissolve the contents. This working
reagent is stable 4 months at 2-8°C or 40 days at room temperature when stored in a dark bottle. Avoid direct sunlight.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
Handle standard carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank
absorbance at 500-550 nm ≥ 0,1, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Note 2 Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma: Stability of the sample for 7 days at 2-8 °C or freezing at -20 °C will keep samples for 3 months.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL Working Reagent
For StandardNote 2 10 µL Standard + 1 mL Working Reagent
For Sample 10 µL Sample + 1 mL Working Reagent

You can prepare several samples simultaneously. Mix and incubate for 10 minutes at 37 °C or for 15 minutes at 15 - 25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 45 minutes after preparation.

PROGRAM SETUP
Program Name: CHOL Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,1000Note 1
Main Filter: 510 nm Normal Low: 120,0000 mg/dL
Sub Filter: None nm Normal High: 200,0000 mg/dL
Program Unit: mg/dL Num of STD: 1Note 2
Aspiration volume: 0800 µL CONC: (value see vial)Note 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,0000 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 900,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0 mg/dL (detection limit) to 900 mg/dL (linearity limit). If the obtained results are greater than 900 mg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

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Application sheet

REF HBL010 HBL010A HBL010M Cholesterol

VOL 2 x 125 mL 8 x 125 mL 8 x 30 mL
Standard 1 x 5 mL 4 x 5 mL - Enzymatic. Colorimetric test
CHOD-POD

REAGENT PREPARATION AND STABILITY

The reagent and standard are ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 510 nm ≥ 0,26, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Note 2 Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma: stability of the sample for 7 days at 2-8 °C or 3 months at -20 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL Reagent
For StandardNote 2 10 µL Standard + 1 mL Reagent
For Sample 10 µL Sample + 1 mL Reagent
You can prepare several samples simultaneously. Mix and incubate for 10 minutes at 37 °C or for 15 minutes at 15 - 25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 45 minutes after preparation.

PROGRAM SETUP
Program Name: CHOLLn Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,2600Note 1
Main Filter: 510 nm Normal Low: 120,0000 mg/dL
Sub Filter: None nm Normal High: 200,0000 mg/dL
Program Unit: mg/dL Num of STD: 1Note 2
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,5210 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 1000,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,521 mg/dL (detection limit) to 1000 mg/dL (linearity limit). If the obtained results are greater than 1000 mg/dL, dilute
the sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

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Application sheet
REF HBL011 HDL Cholesterol
VOL 120 + 40 mL
Standard -
Enzymatic. Colorimetric

REAGENT PREPARATION AND STABILITY

R1 and R2 are ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
The reagents are light sensitive. Do not leave bottles open. Do not freeze the reagents.
R1 and R2: once opened they are stable 60 days at 2-8 °C. The reagent should be a clear solution. If turbidity or precipitation has occurred, the
reagent should be discarded.Note 1

CALIBRATION & QUALITY CONTROL

Use the HDL/LDL calibrator (HBC11) for calibration. The concentration is lot specific and given on the label of the calibrator.Note 2
Control sera are recommended to monitor the performance of assay procedures. Use the HDL/LDL Control kit (HBC10). Note 3 Prepare and measure
these controls the same as samples.
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma (EDTA, citrate, Li Heparin). Fasting and non-fasting samples can be used.

PROCEDURE
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
For Reagent BlankNote 1 10 µL Distilled water + 750 µL R1
For Sample/Calibrator BlankNote 2 10 µL Sample/Calibrator + 750 µL R1
For Sample/Calibrator 10 µL Sample/Calibrator + 750 µL R1
Mix and incubate for exactly 5 minutes at 37 °C. Then add:
For Reagent BlankNote 1 250 µL R2
For Sample/CalibratorNote 2 250 µL R2
Mix and incubate for exactly 5 minutes at 37 °C. Then aspirate to measure.
Thus, for every sample, you need to prepare 2 test tubes: one for the Calibrator/Sample Blank, to measure the background coloration caused
by the sample, and one for the Calibrator/Sample to measure the coloration caused by the reaction. After mixing Calibrator/Sample and R1,
incubate at 37 °C for exactly 5 minutes. Then add R2 only to the Calibrator/Sample tube, mix and incubate for another 5 minutes at 37 °C. Then
aspirate the mixtures in the analyser to measure exactly 10 minutes after adding R1. You can prepare several samples simultaneously, as long as
you respect the incubation times indicated.
Use the illustrations on the next page for guidance to perform this test in a time-efficient way.

PROGRAM SETUP
Program Name: HDLd Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 1,0000Note 1
Main Filter: 578 nm Normal Low: 59,0000 mg/dL
Sub Filter: None nm Normal High: 80,0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0550 µL CONC: (value see vial)Note 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 1,0600 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 184,8000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: SerumNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 1,06 mg/dL (detection limit) to 184,8 mg/dL (linearity limit). If the obtained results are greater than 184,8 mg/dL, dilute
the sample 1:2 with NaCl 9 g/L, repeat the determination, and multiply the result by factor 2.
NOTES
1. The Blank method programmed is Serum! Pay attention, this determines the calculation of the results, not the blank aspiration!
- Aspirate distilled water to adjust the instrument to zero (AD value)
- “Test Blank”  “yes” and aspirate the Reagent Blank (R1 + R2 + distilled water).
- In the Calibrator/Sample menu,
a. “Aspirate Serum” = Calibrator/Sample Blank mixture (=Calibrator/Sample + R1).
b. “Aspirate Standard/Sample” = Calibrator/Sample mixture (=Calibrator/Sample + R1 + R2).
If the blank absorbance is out of range, the instrument will give you a flag.
2. Use the HDL/LDL calibrator (HBC11) for calibration. Enter the concentration values shown on the calibrator vials (HBC11).
3. The control values can be found on the label of the control vial (HBC10).

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Application sheet
REF HBL012 LDL Cholesterol
VOL 120 + 40 mL
Standard -
Enzymatic. Colorimetric.

REAGENT PREPARATION AND STABILITY

R1 and R2 are ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light
and contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
The reagents are light sensitive. Do not leave bottles open. Do not freeze the reagents.
R1 and R2: once opened they are stable for 60 days at 2-8 °C. The reagents should be a clear solution. If turbidity or precipitation has occurred, the
reagent should be discarded.Note 1

CALIBRATION & QUALITY CONTROL

Use the HDL/LDL calibrator (HBC11) for calibration. The concentration is lot specific and given on the label of the calibrator.Note 2
Control sera are recommended to monitor the performance of assay procedures. Use the HDL/LDL Control kit (HBC10). Note 3 Prepare and measure
these controls the same as samples. If control values are found outside the defined range mentioned on the insert of the control, check the
instrument, reagents and calibrator for problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not
meet the acceptable tolerances.

SAMPLES
Serum or plasma (EDTA, citrate). Fasting and non-fasting samples can be used. Do not use plasma containing heparin as anticoagulant.

PROCEDURE
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
For Reagent BlankNote 1 10 µL Distilled water + 750 µL R1
For Sample/Calibrator BlankNote 2 10 µL Sample/Calibrator + 750 µL R1
For Sample/Calibrator 10 µL Sample/Calibrator + 750 µL R1
Mix and incubate for exactly 5 minutes at 37 °C. Then add:
For Reagent BlankNote 1 250 µL R2
For Sample/CalibratorNote 2 250 µL R2
Mix and incubate for exactly 5 minutes at 37 °C. Then aspirate to measure.
Thus for every sample, you need to prepare 2 test tubes: one for the Calibrator/Sample Blank, to measure the background coloration caused
by the sample, and one for the Calibrator/Sample to measure the coloration caused by the reaction. After mixing Calibrator/Sample and R1,
incubate at 37 °C for exactly 5 minutes. Then add R2 only to the Calibrator/Sample tube, mix and incubate for another 5 minutes at 37 °C. Then
aspirate the mixtures in the analyser to measure exactly 10 minutes after adding R1. You can prepare several samples simultaneously as long as
you respect the times mentioned.
Use the illustrations on the next page for guidance to perform this test in a time-efficient way.

PROGRAM SETUP
Program Name: LDLd Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 1,0000Note 1
Main Filter: 578 nm Normal Low: 50,0000 mg/dL
Sub Filter: None nm Normal High: 100,0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0550 µL CONC: (value see vial)Note 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 1,6400 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 250,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: SerumNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 1,64 mg/dL (detection limit) to 250 mg/dL (linearity limit). If the obtained results are greater than 250 mg/dL, dilute the
sample 1:2 with NaCl 9 g/L, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. The Blank method programmed is Serum! Pay attention, this determines the
calculation of the results, not the blank aspiration!
- Aspirate distilled water to adjust the instrument to zero (AD value)
- “Test Blank”  “yes” and aspirate the Reagent Blank (R1 + R2 + distilled water).
- In the Calibrator/Sample menu,
a. “Aspirate Serum” = Calibrator/Sample Blank mixture (=Calibrator/Sample + R1).
b. “Aspirate Standard/Sample” = Calibrator/Sample mixture (=Calibrator/Sample + R1 + R2).
2. Use the HDL/LDL calibrator (HBC11) for calibration. Enter the concentration values shown on the calibrator vials (HBC11).
3. The control values can be found on the label of the HDL/LDL Direct control set vials (HBC10).
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 CY009 CYANSmart
Application sheet

REF HBEL05 Creatine Kinase MB

VOL 60 + 15 mL
CK (NAC & MB) Control 1 x Lyoph.-2 mL Immuno-inhibition. UV. Kinetic

REAGENT PREPARATION AND STABILITY

Working reagent: mix 4 volumes of R1 with 1 volume of R2. After mixing, allow to stand for 30 minutes prior to use. The stability of the working
reagent is 7 days at 2-8 °C or 12 hours at room temperature (15-25 °C).
Control: dissolve the contents in 2 mL of distilled water. Cap vial and mix gently to dissolve the contents. Stability: 8 hours at 15-25 °C, 5 days at
2-8 °C or 1 month at -20 °C. Bring at room temperature for about 30 min before use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank absorbance at 340 nm ≥ 1,20, the reagent should be
discarded.Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor.
Control sera are recommended to monitor the performance of assay procedures. Use the CK (NAC&MB) control (HBC08) included in the kit.Note 2
Prepare and measure these controls the same as samples. If control values are found outside the defined range, check the instrument, reagents
and calibrator for problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable
tolerances.

SAMPLES
Serum free of hemolysis or heparin plasma: stability 7 days at 2-8 °C, protected from light. CK-MB activity decreases a 10% after 24 hours at 4 °C or
1 hour at 25 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For BlankNote 1 1,00 mL Working reagent (R1 + R2)
For Sample/(Calibrator) 40 µL Sample/(Calibrator) + 1,00 mL Working reagent (R1 + R2)
Mix and incubate for 10 minutes at room temperature. After the incubation time, aspirate and measure the sample.

PROGRAM SETUP
Program Name: CKMBL Blank Low: 0,0000Note 1
Program Method: Kinetic Blank High: 1,2000Note 1
Main Filter: 340 nm Normal Low: 1,0000 U/L
Sub Filter: None nm Normal High: 24,0000 U/L
Program Unit: U/L Num of STD: 0
Aspiration volume: 0800 µL CONC: 0,0000 U/L
Delay Time: 10 sec Factor: 8255,0000
Test time: 300 sec Control N min: Enter valueNote 2 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 2 U/L
Linearity Min: 1,9000 U/L Control P min: Enter valueNote 2 U/L
Linearity Max: 318,0000 U/L Control P max: Enter valueNote 2 U/L
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 1,9 U/L (detection limit) to 318 U/L (linearity limit). If the obtained results are greater than 318 U/L, dilute the sample 1:2
with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. The control values can be found on the label of the control vial.

2021-09 (5.0) - Replaces all previous versions

20/49
Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
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 CY009 CYANSmart
Application sheet

REF HBEL03 Creatine Kinase NAC

VOL 60 + 15 mL
Standard -
NAC Activated. UV. Kinetic

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of reagent 1 with 1 volume of reagent 2. The stability of the working reagent is 2 weeks at 2-8 °C or 48 hours at room temperature
(15-25 °C).
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank absorbance at 340 nm ≥ 1,0 the reagent should be
discarded. Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls Specific
(HBC01-S, HBC02-S). Also a CK (NAC & MB) Control (HBC08) is available.Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum free of hemolysis or heparin plasma: stability 7 days at 2-8 °C, protected from light. The creatinine kinase activity decreases 10% after 1 day
at 2-5 °C or after 1 hour at 15-25 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For BlankNote 1 1,00 mL Working reagent (R1 + R2)
For Sample/(Calibrator)Note 2 20 µL Sample/(Calibrator) + 1,00 mL Working reagent (R1 + R2)
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working solution to
the sample/calibrator. Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: CKNACL Blank Low: 0,0000Note 1
Program Method: Kinetic Blank High: 1,0000Note 1
Main Filter: 340 nm Normal Low: 34,0000 U/L
Sub Filter: None nm Normal High: 195,0000 U/L
Program Unit: U/L Num of STD: 0Note 2
Aspiration volume: 0800 µL CONC: 0,0000Note 2 U/L
Delay Time: 120 sec Factor: 8095,0000Note 2
Test time: 090 sec Control N min: Enter valueNote 3 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 U/L
Linearity Min: 2,1200 U/L Control P min: Enter valueNote 3 U/L
Linearity Max: 2000,0000 U/L Control P max: Enter valueNote 3 U/L
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 2,12 U/L (detection limit) to 2000 U/L (linearity limit). If the obtained results are greater than 2000 U/L, dilute the sample
1:10 with saline solution, repeat the determination, and multiply the result by factor 10.

NOTES
1. Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively, you can program
and aspirate distilled water as blank, with values for Blank Low: 0,0000 and Blank High: 0,0100. If the blank absorbance is out of range, the
instrument will give you a flag.
2. Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

2021-09 (5.0) - Replaces all previous versions

21/49
Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
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 CY009 CYANSmart
Application sheet

REF HB0080 HB0080A HB0080M Creatinine

VOL 2 x 125 mL 8 x 125 mL 8 x 30 mL
Standard 1 x 5 mL 4 x 5 mL - Jaffé. Colorimetric. Kinetic without
deproteinization

REAGENT PREPARATION AND STABILITY

Mix proportionally 1:1 R1 Picric Reagent and R2 Alkaline Reagent. The working reagent is stable for 10 days at 15-25 °C.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-25 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 510 nm ≥ 1,80, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
- Use serum or heparinized plasma. Creatinine is stable 24 hours at 2-8 °C.
- Urine diluted 1:50 with distilled water and multiply the results by 50 (dilution factor). Creatinine is stable for 7 days at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For BlankNote 1 1,00 mL Working reagent
For StandardNote 2 100 µL Standard + 1,00 mL Working reagent
For Sample 100 µL Sample + 1,00 mL Working reagent
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working solution to
the sample/calibrator. Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: CRE Blank Low: 0,0000Note 1
Program Method: Two Point Blank High: 1,8000Note 1
Main Filter: 510 nm Normal Low: 0,6000Note 4 mg/dL
Sub Filter: None nm Normal High: 1,4000Note 4 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0500 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 030 sec Factor: 0,0000
Test time: 060 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,1150 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 15,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,115 mg/dL (detection limit) to 15 mg/dL (linearity limit). If the obtained results are greater than 15 mg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).Enter the concentration values shown on the calibrator vials.
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum or plasma samples. For urine, please check the insert for the values and sample preparation.

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Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
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 CY009 CYANSmart
Application sheet

REF HBE11 G6-PDH

VOL 100 + 20 mL
Standard -
Enzymatic. UV

REAGENT PREPARATION AND STABILITY

- R1- R4: ready to use
- R2- R3: Reconstitute the contents of each bottle with 2 mL of distilled water.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
R1 and R4 are stable until the expiration date on the label when stored tightly closed at 2-8 °C, protected from light and contaminations prevented
during their use. After reconstitution, R2 and R3 are stable for 4 weeks at 2-8 °C. Do not use reagents over the expiration date.

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor.
Control sera are recommended to monitor the performance of assay procedures. A 2 level G6-PDH Control (HBC09) is available.Note 3 Control
HBC09 does not need a digitonin pretreatment (= precipitation with Reagent 4).
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Erythrocytes: Preparation: Wash 0,2 mL of blood with 2 mL aliquots of 0,9% NaCl solution. Centrifuge after each wash for 10 min at around 3000
rpm. Repeat 3 times. Suspend the washed centrifuged erythrocytes in 0,5 mL of R4 and let stand for 15 min at 4 °C and then centrifuge again. Use
the supernatant (= hemolysate) in the assay within 2 hours.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Sample 15 µL Hemolysate + 1000 µL R1 + 30 µL R2
Mix and incubate at 37 °C for 5 minutes. Then add R3:
For Sample 15 µL R3
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of R3 to the sample. Wait
with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time, the instrument asks: “Press
PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: G6PDH Blank Low: 0,0000Note 1
Program Method: Kinetic Blank High: 0,0100Note 1
Main Filter: 340 nm Normal Low: 1170,0000Note 4 U/L
Sub Filter: None nm Normal High: 2455,0000Note 4 U/L
Program Unit: U/L Num of STD: 0
Aspiration volume: 0800 µL CONC: 0,0000 U/L
Delay Time: 003 sec Factor: 33650,0000
Test time: 090 sec Control N min: Enter valueNote 3 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 U/L
Linearity Min: 154,0000 U/L Control P min: Enter valueNote 3 U/L
Linearity Max: 4000,0000 U/L Control P max: Enter valueNote 3 U/L
Blank: Water Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 154 U/L (detection limit) to 4000 U/L (linearity limit). If the obtained results are greater than 4000 U/L, dilute the
hemolysate 1:10 by adding 0,2 mL hemolysate to 1,8 mL 0,9% NaCl, repeat the determination, and multiply the result by factor 10.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Calibration by means of a Factor.
3. The control values can be found on the label of the control vial.
4. These values are for erythrocytes (U/L). To obtain results in U/g HGB, divide the results of this analysis with the results for Hemoglobin (g/L) for
that sample.

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 CY009 CYANSmart
Application sheet

REF HB009 Glucose Lyo

VOL 2 x 125 mL
Standard 1 x 5 mL Enzymatic-colorimetric test.
GOD-POD

REAGENT PREPARATION AND STABILITY

Dissolve the contents of one bottle R2 Enzymes in one bottle R1 Buffer. Cap and mix gently to dissolve the contents. This working reagent is stable
1 month after constitution at 2-8°C or 7 days at room temperature (15-25°C).
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8°C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 490-550 nm ≥ 0,10, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma, free of hemolysis and CSF. Serum should be removed from the clot as quickly as possible. Glucose is stable for 3 days at 2-8°C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL Reagent
For StandardNote 2 10 µL Standard + 1 mL Reagent
For Sample 10 µL Sample + 1 mL Reagent
You can prepare several samples simultaneously. Mix and incubate for 10 minutes at 37 °C or for 20 minutes at 15-25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 30 minutes after preparation.

PROGRAM SETUP
Program Name: GLUC Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,1000Note 1
Main Filter: 510 nm Normal Low: 60,0000Note 4 mg/dL
Sub Filter: None nm Normal High: 110,0000Note 4 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 001 sec Factor: 0,0000Note 2
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,0000 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 500,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0 mg/dL (detection limit) to 500 mg/dL (linearity limit). If the obtained results are greater than 500 mg/dL, dilute the
sample 1:2 with NaCl 9 g/L, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum or plasma samples. For CSF samples, please check the insert for the values and sample preparation.

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 CY009 CYANSmart
Application sheet

REF HBL04 HBL04A HBL04M Glucose

VOL 2 x 125 mL 8 x 125 mL 8 x 30 mL
Standard 1 x 5 mL 4 x 5 mL - Enzymatic. Colorimetric
GOD-POD

REAGENT PREPARATION AND STABILITY

Reagent and standard are ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
Handle standards very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 510 nm ≥ 0,32, the reagent should be discarded. Do not use the product if deterioration or contamination is suspected or
beyond the expiration date. Dispose unused or deteriorated product and waste in compliance with local regulations.Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Fluoride plasma, free of hemolysis. Plasma should be isolated in blood tubes containing sodium fluoride (NaF) to inhibit glycolysis. In fluoride
plasma, the glucose concentration is stable for up to 3 days at room temperature. For fasting glucose determination, fasting for at least 12 hours is
recommended before sample collection.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1,00 mL Reagent
For StandardNote 2 10 µL Standard + 1,00 mL Reagent
For Sample 10 µL Sample + 1,00 mL Reagent
You can prepare several samples simultaneously. Mix and incubate for 10 minutes at 37 °C or for 15 minutes at 15-25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 40 minutes after preparation.

PROGRAM SETUP
Program Name: GLUCLn Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,3200Note 1
Main Filter: 510 nm Normal Low: 74,0000 mg/dL
Sub Filter: None nm Normal High: 100,0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 14,1000 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 420,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 14,1 mg/dL (detection limit) to 420 mg/dL (linearity limit). If the obtained results are greater than 420 mg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

2021-09 (5.0) - Replaces all previous versions

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Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
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 CY009 CYANSmart
Application sheet

REF HBEL06 HBEL061 γ-GT

VOL 240 + 60 mL 60 +15 mL
Standard - -
Carboxy substrate. Colorimetric. Kinetic

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of R1 (Buffer) with 1 volume of R2 (Substrate). The stability of this working reagent is 21 days at 2-8 °C or 5 days at room temperature
(15-25 °C).
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank absorbance at 405 nm ≥ 1,80, the reagent should be
discarded.Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor. Note 2 Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum: γ-GT is stable for at least 3 days at 2-8 °C, 8 hours at 15-25 °C and 1 month at -20 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For BlankNote 1 1,00 mL Working reagent (R1 + R2)
For Sample/(Calibrator)Note 2 100 µL Sample/(Calibrator) + 1,00 mL Working reagent (R1 + R2)
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working reagent to
the sample/(calibrator). Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: GGTL Blank Low: 0,0000Note 1
Program Method: Kinetic Blank High: 1,8000Note 1
Main Filter: 405 nm Normal Low: 7,0000 U/L
Sub Filter: None nm Normal High: 50,0000 U/L
Program Unit: U/L Num of STD: 0Note 2
Aspiration volume: 0800 µL CONC: 0,0000Note 2 U/L
Delay Time: 060 sec Factor: 1190,0000Note 2
Test time: 090 sec Control N min: Enter valueNote 3 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 U/L
Linearity Min: 2,0000 U/L Control P min: Enter valueNote 3 U/L
Linearity Max: 300,0000 U/L Control P max: Enter valueNote 3 U/L
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 2 U/L (detection limit) to 300 U/L (linearity limit). If the obtained results are greater than 300 U/L, dilute the sample 1:10
with saline solution, repeat the determination, and multiply the result by factor 10.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD (1) and
CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

2021-02 (4.0) - Replaces all previous versions

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 CY009 CYANSmart
Application sheet

REF HBEL010 HBEL010A HBEL010M

1 x 240 + 1 x 6 x 120 + 3 x 6 x 30 + 3 x GOT (AST)
VOL
60 mL 60 mL 15 mL
Standard - - - NADH. UV. Kinetic. According to IFCC

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of R1 (buffer) with 1 volume of R2 (substrate). The stability of the working reagent is 24 hours at 15 - 25 °C or 14 days at 2 - 8 °C.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
The reagent should be a clear solution.If turbidity or precipitation has occurred or if blank absorbance at 340 nm < 1,00, the reagent should be
discarded. Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor. Note 2 Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma: stability 2 days at 2 - 8 °C. Fasting of at least 12 hours is recommended before sample collection.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For BlankNote 1 1,00 mL Working reagent (R1 + R2)
For Sample/(Calibrator)Note 2 100 µL Sample/(Calibrator) + 1,00 mL Working reagent (R1 + R2)
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working reagent to
the sample/(calibrator). Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this
time, the instrument asks “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: GOTL Blank Low: 1,0000Note 1
Program Method: Kinetic Blank High: 2,5000Note 1
Main Filter: 340 nm Normal Low: 3,1200 U/L
Sub Filter: None nm Normal High: 38,0000 U/L
Program Unit: U/L Num of STD: 0Note 2
Aspiration volume: 0800 µL CONC: 0,0000Note 2 U/L
Delay Time: 060 sec Factor: 1750,0000Note 2
Test time: 090 sec Control N min: Enter valueNote 3 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 U/L
Linearity Min: 3,1200 U/L Control P min: Enter valueNote 3 U/L
Linearity Max: 260,0000 U/L Control P max: Enter valueNote 3 U/L
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 3,12 U/L (detection limit) to 260 U/L (linearity limit). If the obtained results are greater than 260 U/L, dilute the sample
1:10 with saline solution, repeat the determination, and multiply the result by factor 10.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD (1) and
CONC (as mentioned on the insert provided with HBC03).Enter the concentration values shown on the calibrator vials.
3. The control values can be found on the control sheets, delivered together with the control vials.

2021-02 (4.0) - Replaces all previous versions

27/49
Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
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 CY009 CYANSmart
Application sheet

REF HBEL020 HBEL020A HBEL020M

1 x 240 + 1 x 6 x 120 + 3 x 6 x 30 + 3 x GPT (ALT)
VOL
60 mL 60 mL 15 mL
Standard - - - NADH. UV. Kinetic. According to IFCC

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of R1 (buffer) with 1 volume of R2 (substrate). The stability of the working reagent is 24 hours at 15-25 °C or 4 weeks at 2-8 °C.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank absorbance at 340 nm < 1,00, the reagent should be
discarded.Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor. Note 2 Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma: stability 1 day at 2-8 °C. Fasting of at least 12 hours is recommended before sample collection.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For BlankNote 1 1,00 mL Working reagent (R1 + R2)
For Sample/(Calibrator)Note 2 100 µL Sample/(Calibrator) + 1,00 mL Working reagent (R1 + R2)
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working reagent to
the sample/calibrator. Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: GPTL Blank Low: 1,0000Note 1
Program Method: Kinetic Blank High: 2,5000Note 1
Main Filter: 340 nm Normal Low: 3,0000 U/L
Sub Filter: None nm Normal High: 40,0000 U/L
Program Unit: U/L Num of STD: 0Note 2
Aspiration volume: 0800 µL CONC: 0,0000Note 2 U/L
Delay Time: 060 sec Factor: 1750,0000Note 2
Test time: 090 sec Control N min: Enter valueNote 3 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 U/L
Linearity Min: 3,0000 U/L Control P min: Enter valueNote 3 U/L
Linearity Max: 260,0000 U/L Control P max: Enter valueNote 3 U/L
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 3 U/L (detection limit) to 260 U/L (linearity limit). If the obtained results are greater than 260 U/L, dilute the sample 1:10
with saline solution, repeat the determination, and multiply the result by factor 10.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD (1) and
CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

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Application sheet

REF HT001 HbA1c Turbi

VOL 30 + 10 mL
Standard -
Turbidimetric

REAGENT PREPARATION AND STABILITY

Reagent 1, 2 and 3 are ready to use. Latex may sediment. Mix reagents gently before use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
Reagent 1 and 2 are stable for at least one month after opening when stored at 2 - 8 °C. Reagent deterioration: alterations in the physical appearance of
the reagents or values of control materials outside of the manufacturer's acceptable range may be an indication of reagent instability.Note 1
CALIBRATION & QUALITY CONTROL
Use the HbA1c Calibrator set (HT001S) Program the values mentioned on the vials in the method programming. Use saline solution (9 g/L NaCl) or distilled
water as STD 1, with value 0,001.Note 2
Control sera are recommended to monitor the performance of assay procedures. HbA1c Control (HT001C) is available.Note 3 Prepare and measure these
controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish its own
QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Human whole blood. Special preparation of the patients is unnecessary. Fasting samples are not required. No special additives or preservatives other than
anticoagulants are required. Collect venous blood with EDTA using aseptic technique. HbA1c in whole blood collected with EDTA is stable for one week at
2 - 8 °C.
To determine HbA1c, a hemolysate must be prepared for each sample as well as for the HbA1c Calibrator (Level 1 to 4) and HbA1c Control (if required):
1. Dispense 1 mL of Reagent 3 into labelled test tubes (glass or plastic). Also provide test tubes for the calibrator and control.
2. Add 20 µL of well mixed whole blood (sample, calibrator, control) in the appropriate labelled test tube. Mix.
3. Allow to stand for 5 minutes or until complete lysis is evident. Hemolysates may be stored up to 10 days at 2 - 8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Mix reagents gently and pipette into a test tube:
For Blank 540 µL Reagent 1
For Calibrators/Sample 15 µL Calibrator/Sample (hemolyzed) + 540 µL Reagent 1
Mix and incubate for exactly 5 minutes at 37 °C. Then add:
For Blank 180 µL Reagent 2
For Calibrators/Sample 180 µL Reagent 2
Mix and incubate for exactly 5 minutes at 37 °C. Then aspirate to measure.
You can prepare several samples simultaneously, as long as you respect the incubation times indicated.

PROGRAM SETUP
Program Name: HbA1c Normal Low: 2,0000Note 4 %
Program Method: End Point Normal High: 6,0000Note 4 %
Main Filter: 620 nm Num of STD: 5Note 2
Sub Filter: None nm STD 1 CONC: 0,0010Note 2 %
Program Unit: % STD 2 CONC: (=CAL1)Note 2 %
Aspiration volume: 0500 µL STD 3 CONC: (=CAL2)Note 2 %
Delay Time: 001 sec STD 4 CONC: (=CAL3)Note 2 %
Test time: 003 sec STD 5 CONC: (=CAL4)Note 2 %
Dilution Factor: 1,0000 Factor: 0,0000Note 2
Linearity Min: 2,0000 % Control N min: Enter valueNote 3 %
Linearity Max: 16,0000 % Control N max: Enter valueNote 3 %
Blank: Reagent Control P min: Enter valueNote 3 %
Num of Blank: 1 Control P max: Enter valueNote 3 %
Blank Low: 0,0000Note 1 Cuvette Temp: 37 °C
Blank High: 2,0000Note 1

MEASURING RANGE
This method is linear from 2,0 % (detection limit) to 16,0 % (linearity limit).

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Enter the concentration values shown on the calibrator vials. STD1 corresponds with a saline solution of 9 g/L or distilled water, STD 2 corresponds to
calibrator 1; STD 3 to calibrator 2; STD 4 to calibrator 3 and STD 5 to calibrator 4.
3. The control values can be found on the label of the control vial.
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Application sheet

REF HB011 Hemoglobin

VOL 4 x 5 mL
Standard -
Drabkin. Colorimetric.

REAGENT PREPARATION AND STABILITY

Working reagent :
- 4,9 mL distilled water + 2 drops of reagent and mix.
Or:
- 245 mL distilled water + 5 mL of reagent and mix.
The diluted reagent (working reagent) is stable 2 months at 2-8 °C, protected from sunlight.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank absorbance at 540 nm ≥ 0,01, the reagent should be
discarded. Note 1

CALIBRATION & QUALITY CONTROL

Use the Hemoglobin Calibrator (HBS02). Note 2
Hemoglobin Calibrator (HBS02) is ready to use. Hemoglobin Calibrator (HBS02) is stable at 2-8 °C up to the date of expiration as specified, when
stored tightly closed, protected from light and contaminations, prevented during its use.
Control sera are recommended to monitor the performance of assay procedures. Note 3
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Capillary or venous blood. Use anticoagulants like EDTA, heparin or oxalate. Stability 7 days at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 2,5 mL Working Reagent
For Calibrator 10 µL Calibrator + 2,5 mL Working Reagent
For Sample 10 µL Sample + 2,5 mL Working Reagent
Mix and incubate for 3 minutes at 15-25 °C. Then aspirate to measure. You can prepare several samples simultaneously, as long as you respect the
incubation times indicated.

PROGRAM SETUP
Program Name: HGB Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,0100Note 1
Main Filter: 546 nm Normal Low: 12,0000 g/dL
Sub Filter: None nm Normal High: 18,0000 g/dL
Program Unit: g/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: 15,0000Note 2 g/dL
Delay Time: 001 sec Factor: 0,0000Note 2
Test time: 003 sec Control N min: Enter valueNote 3 g/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 g/dL
Linearity Min: 0,1000 g/dL Control P min: Enter valueNote 3 g/dL
Linearity Max: 20,0000 g/dL Control P max: Enter valueNote 3 g/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,1 g/dL (detection limit) to 20 g/dL (linearity limit). If the obtained results are greater than 20 g/dL, dilute the sample
1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Use the Hemoglobin Calibrator (HBS02). Enter the concentration value shown on the calibrator vial.
3. The control values can be found on the control sheets, delivered together with the control vials.

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Application sheet
REF HB012 Iron
VOL 4 x 50 mL
Standard 1 x 10 mL
Ferrozine. Colorimetric

REAGENT PREPARATION AND STABILITY

R3 is ready to use.
Add the contents of one tube R2 reductant to the contents of one bottle R1 buffer. Cap and mix gently to dissolve content. This working reagent
is stable for 3 months at 2-8 °C or 1 month at room temperature (15-25 °C).
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if the
reagent blanc absorbance is out of range, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls Specific
(HBC01-S, HBC02-S). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or heparinized plasma. Hemolysis interferes with the test. Separate from the cells as rapidly as possible. The iron is stable up to 7 days stored
at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
For Reagent BlankNote 1 200 µL Distilled water + 1 mL Working reagent + 1 drop R3
For Standard BlankNote 2 200 µL Standard + 1 mL Working reagent
For Sample BlankNote 2 200 µL Sample + 1 mL Working reagent
For Standard 200 µL Standard + 1 mL Working reagent + 1 drop R3
For Sample 200 µL Sample + 1 mL Working reagent + 1 drop R3
Mix and incubate for 10 minutes at 15-25 °C. After the incubation time, aspirate and measure all the samples within 30 min after preparation.
Thus, for every sample, you need to prepare 2 test tubes: one for measuring the sample blank (background coloration) and one for measuring
the real sample coloration.
PROGRAM SETUP
Program Name: IRON Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,1000Note 1
Main Filter: 578 nm Normal Low: 40,0000 μg/dL
Sub Filter: None nm Normal High: 175,0000 μg/dL
Program Unit: μg/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 μg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 μg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 μg/dL
Linearity Min: 0,8500 μg/dL Control P min: Enter valueNote 3 μg/dL
Linearity Max: 1000,0000 μg/dL Control P max: Enter valueNote 3 μg/dL
Blank: SerumNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,85 μg/dL (detection limit) to 1000 μg/dL (linearity limit). If the obtained results are greater than 1000 μg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.
NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. The Blank method programmed is Serum! Pay attention, this
determines the calculation of the results, not the blank aspiration!
- Aspirate distilled water to adjust the instrument to zero (AD value)
- “Test Blank”  “yes” and aspirate the Reagent Blank.
- In the standard/sample menu,
a. First: “Aspirate Serum” = Standard/Sample Blank.
b. Secondly: “Aspirate Standard/Sample” = Standard/Sample (including 1 drop of R3).
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Program
NUM of STD (1) and CONC (as mentioned on the insert provided with HBC03-S).
3. The control values can be found on the control sheets, delivered together with the control vials.

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Application sheet

REF HBEL04 HBEL041 Lactate Dehydrogenase

VOL 240 + 60 mL 60 + 15 mL
Standard - -
Pyruvate DGKC. UV. Kinetic.

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of R1 (buffer) with 1 volume of R2 (substrate). The stability of working reagent is 15 days at 2-8 °C or 5 days at room temperature
(15-25 °C).
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank absorbance at 340 nm < 1,00, the reagent should be
discarded. Note 1

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor. Note 2 Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum, separated from the cells as rapidly as possible. Do not use oxalates as anticoagulants since they inhibit the enzyme. Do not use hemolyzed
samples. LDH in the serum is stable for 2 days at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For BlankNote 1 0,9 mL Working reagent (R1 + R2)
For Sample/(Calibrator)Note 2 15 µL Sample/(Calibrator) + 0,9 mL Working reagent (R1 + R2)
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working solution to
the sample/calibrator. Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: LDHL Blank Low: 1,0000Note 1
Program Method: Kinetic Blank High: 2,5000Note 1
Main Filter: 340 nm Normal Low: 230,0000 U/L
Sub Filter: None nm Normal High: 460,0000 U/L
Program Unit: U/L Num of STD: 0Note 2
Aspiration volume: 0700 µL CONC: 0,0000Note 2 U/L
Delay Time: 060 sec Factor: 9690,0000Note 2
Test time: 090 sec Control N min: Enter valueNote 3 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 U/L
Linearity Min: 3,4200 U/L Control P min: Enter valueNote 3 U/L
Linearity Max: 1600,0000 U/L Control P max: Enter valueNote 3 U/L
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 3,42 U/L (detection limit) to 1600 U/L (linearity limit). If the obtained results are greater than 1600 U/L, dilute the sample
1:10 with saline solution, repeat the determination, and multiply the result by factor 10.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD (1) and
CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

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Application sheet

REF HBE09 Lipase

VOL 4 x 10 mL
Calibrator 1 x Lyoph. - 1 mL
Enzymatic. Colorimetric. Kinetic

REAGENT PREPARATION AND STABILITY

R1 and R2: ready to use. Stability after opening 90 days at 2-8 °C.
R2: mix gently before use. Note 4
Calibrator: reconstitute the contents of one vial with 1 mL of distilled water. Mix gently until complete solution. Stability: 7 days at 2-8 °C. Divide
calibrator solution into small volumes and freeze. Stability: 3 months at -20 °C.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
The reagent should be a clear solution. If turbidity or precipitation has occurred or if blank absorbance at 578 nm ≥ 1,4, the reagent should be
discarded. R2 is a turbid orange-colored micro-emulsion, discard if turning to red. Note 1

CALIBRATION & QUALITY CONTROL

Use the calibrator included in the kit.Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls Specific
(HBC01-S, HBC02-S). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Fresh serum or plasma with sodium citrate, EDTA or heparin. Stability: 2 days at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Reagent BlankNote 1 10 µL Distilled water + 1 mL Reagent 1 + 200 µL Reagent 2
For CalibratorNote 2 10 µL Calibrator + 1 mL Reagent 1 + 200 µL Reagent 2
For Sample 10 µL Sample + 1 mL Reagent 1 + 200 µL Reagent 2
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working reagent to
the sample/calibrator. Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: LIPASE Blank Low: 0,0000Note 1
Program Method: Kinetic Blank High: 1,4000Note 1
Main Filter: 578 nm Normal Low: 5,0000 U/L
Sub Filter: None nm Normal High: 38,0000 U/L
Program Unit: U/L Num of STD: 1Note 2
Aspiration volume: 0800 µL CONC: value: see vialNote 2 U/L
Delay Time: 060 sec Factor: 0,0000Note 2
Test time: 090 sec Control N min: Enter valueNote 3 U/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 U/L
Linearity Min: 5,0000 U/L Control P min: Enter valueNote 3 U/L
Linearity Max: 250,0000 U/L Control P max: Enter valueNote 3 U/L
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 5 U/L (detection limit) to 250 U/L (linearity limit). If the obtained results are greater than 250 U/L, dilute the sample 1:10
with saline solution, repeat the determination, and multiply the result by factor 10.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. Use the calibrator included in the kit. Enter the concentration values shown on the calibrator vials. Alternatively, you can use the Biochemistry
Calibrator Specific (HBC03-S) for calibration. Program NUM of STD (1) and CONC (as mentioned on the insert provided with HBC03-S).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. In some storage conditions (lower than the one indicated) a precipitate may appear in the vial that will not influence the reagent performance.
However, it is recommended to re-suspend the product with a slight rotation.

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Application sheet

REF HB0320 HB0320A HB0320M Magnesium

VOL 2 x 125 mL 8 x 125 mL 8 x 30 mL
Standard 1 x 5 mL 4 x 5 mL -
Xylidyl Blue - EGTA. Colorimetric

REAGENT PREPARATION AND STABILITY

The reagent and standard are ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 25 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear blue solution. If turbidity or precipitation has occurred or
if blank absorbance at 510 nm ≥1,5, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
- Serum, heparinized plasma, free of hemolysis and separated from cells as rapidly as possible. Do not use oxalates, citrate or EDTA as anticoagulant.
Stability: 5 days at 4 - 8 °C.
- Urine: collect 24 hours urine specimen in a magnesium free container. Urine should be acidified to pH 1 with HCl. If urine is cloudy, warm the
specimen to 60 °C for 10 minutes to dissolve precipitates. Dilute 1:10 with distilled water and multiply the result by 10. Stability: 3 days at 4 - 8
°C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL Reagent
For StandardNote 2 10 µL Standard + 1 mL Reagent
For Sample 10 µL Sample + 1 mL Reagent
You can prepare several samples simultaneously. Mix and incubate for 3 minutes at 37 °C or for 5 minutes at 15-25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 45 minutes after preparation.

PROGRAM SETUP
Program Name: MgXB Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 1,5000Note 1
Main Filter: 510 nm Normal Low: 1,6000Note 4 mg/dL
Sub Filter: None nm Normal High: 2,5000Note 4 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,0500 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 6,6000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 3

MEASURING RANGE
This method is linear from 0,05 mg/dL (detection limit) to 6,6 mg/dL (linearity limit). If the obtained results are greater than 6,6 mg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD (1) and CONC (as mentioned on the insert
provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum or plasma samples. For urine, please check the insert for the values and sample preparation.

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Application sheet

REF HB014 Phosphorus

VOL 2 x 125 mL
Standard 1 x 5 mL
Phosphomolybdate. UV.

REAGENT PREPARATION AND STABILITY

Reagent and standard are ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 340 nm ≥ 0,54, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls Specific
(HBC01-S, HBC02-S). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
- Serum or plasma, free of hemolysis and separated from cells as rapidly as possible. Stability: 7 days at 2-8 °C.
- Urine: collect the specimen into a bottle containing 10 mL of 10% v/v hydrochloric acid (HCl) to avoid phosphate precipitations. Adjust to pH 2.
Dilute the sample 1:10 with distilled water. Mix. Multiply the result by 10 (dilution factor). Stability: 10 days at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL Reagent
For StandardNote 2 10 µL Standard + 1 mL Reagent
For Sample 10 µL Sample + 1 mL Reagent
Mix and incubate for 5 minutes at 37 °C. Then aspirate to measure. You can prepare several samples simultaneously, as long as you respect the
incubation times indicated.

PROGRAM SETUP
Program Name: PHOSPH Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,5400Note 1
Main Filter: 340 nm Normal Low: 2,5000Note 4 mg/dL
Sub Filter: None nm Normal High: 5,0000Note 4 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,0000 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 35,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0 mg/dL (detection limit) to 35 mg/dL (linearity limit). If the obtained results are greater than 35 mg/dL, dilute the sample
1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Program
NUM of STD (1) and CONC (as mentioned on the insert provided with HBC03-S).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum or plasma samples. For urine, please check the insert for the values and sample preparation.

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Application sheet

REF HB015 Potassium Prec

VOL 2 x 50 mL
Standard 1 x 3 mL
NaTPB. Colorimetric. Precipitation

REAGENT PREPARATION AND STABILITY

Working reagent: Shake R2 (NaOH) before use. Mix proportionally 1:1 Reagent 1 and Reagent 2. After mixing, allow to stand for 30 minutes prior
to use. Before each use, the working reagent must be shaken. The working reagent is stable for 7 days at 15-25 °C and 30 days at 2-8 °C.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
Handle standard very carefully to prevent contamination. Do not freeze or expose to elevated temperatures. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls Specific
(HBC01-S, HBC02-S). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Non hemolytic serum or heparin plasma.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
PRECIPITATION STEP:
For Sample 50 µL Sample + 500 µL Reagent 3
Mix carefully. Centrifuge at high speed (± 5000 rpm) for 5-10 minutes. Separate the clear supernatant in a new test tube.
TEST STEP: pipette into a cuvette:
For Blank 1,0 mL Working reagent (R1 + R2)
For StandardNote 2 100 µL Standard + 1,0 mL Working reagent (R1 + R2)
For Sample 100 µL Supernatant + 1,0 mL Working reagent (R1 + R2)
You can prepare several samples simultaneously. To produce a homogeneous turbidity, the standard or the clear supernatant must be added to
the surface of the working reagent in the test tube. Mix each test tube carefully before proceeding to the next sample. Mix and allow to stand for
5 min. After the incubation time, aspirate and measure all the samples within 30 min after addition of the working solution.

PROGRAM SETUP
Program Name: POT Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 2,5000Note 1
Main Filter: 578 nm Normal Low: 3,6000 mEq/L
Sub Filter: None nm Normal High: 5,5000 mEq/L
Program Unit: mEq/L Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mEq/L
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mEq/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mEq/L
Linearity Min: 2,0000 mEq/L Control P min: Enter valueNote 3 mEq/L
Linearity Max: 10,0000 mEq/L Control P max: Enter valueNote 3 mEq/L
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 2,0 mEq/L (detection limit) to 10,0 mEq/L (linearity limit). If the obtained results are greater than 10,0 mEq/L, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Program
NUM of STD (1) and CONC (as mentioned on the insert provided with HBC03-S).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum samples. For plasma, please check the insert for the values.

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Application sheet

REF HB016 Sodium Prec

VOL 60 mL
Standard 1 x 2 mL
Mg-Uranylacetate. Colorimetric.

REAGENT PREPARATION AND STABILITY

Reagents are ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
Handle standard very carefully to prevent contamination. Precipitating solution becomes discolored when exposed to the light. Store protected
from light. A slight turbidity does not affect the determination. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls Specific
(HBC01-S, HBC02-S). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
PRECIPITATION STEP:
For Blank -
For StandardNote 2 20 µL Standard + 1,0 mL Reagent 2
For Sample 20 µL Sample + 1,0 mL Reagent 2
Mix well. Incubate for 5 min. Then shake intensively for at least 30 sec. Then incubate for 30 min.
Centrifuge for 5-10 min at 5000 rpm. Collect the supernatant in a new test tube
TEST STEP: pipette into a cuvette:
For Blank 20 µL Reagent 2 + 1,0 mL Reagent 1
For Standard 20 µL Supernatant + 1,0 mL Reagent 1
For Sample 20 µL Supernatant + 1,0 mL Reagent 1
You can prepare several samples simultaneously. Mix and incubate for 5 minutes at 15-25 °C (room temperature). After the incubation time,
aspirate and measure the samples within 30 minutes after preparation.

PROGRAM SETUP
Program Name: SOD Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 2,5000Note 1
Main Filter: 405 nm Normal Low: 135,0000 mEq/L
Sub Filter: None nm Normal High: 155,0000 mEq/L
Program Unit: mEq/L Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mEq/L
Delay Time: 001 sec Factor: 0,0000Note 2
Test time: 003 sec Control N min: Enter valueNote 3 mEq/L
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mEq/L
Linearity Min: 49,0000 mEq/L Control P min: Enter valueNote 3 mEq/L
Linearity Max: 300,0000 mEq/L Control P max: Enter valueNote 3 mEq/L
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 49 mEq/L (detection limit) to 300 mEq/L (linearity limit). If the obtained results are greater than 300 mEq/L, dilute the
sample 1:2 with distilled water, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Program
NUM of STD (1) and CONC (as mentioned on the insert provided with HBC03-S).
3. The control values can be found on the control sheets, delivered together with the control vials.

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Application sheet

REF HB0190 HB0190A HB0190M Total Protein

VOL 2 x 125 mL 8 x 125 mL 8 x 30 mL
Standard 1 x 5 mL 4 x 5 mL -
Biuret. Colorimetric

REAGENT PREPARATION AND STABILITY

The reagent and standard are ready for use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 546 nm ≥ 0,22, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range mentioned on the insert of the control, check the instrument, reagents and calibrator for
problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or heparinized plasma: stability: 1 month at 2-8 °C

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL R1 Biuret
For StandardNote 2 25 µL Standard + 1 mL R1 Biuret
For Sample 25 µL Sample + 1 mL R1 Biuret

You can prepare several samples simultaneously. Mix and incubate for 5 minutes at 37 °C or for 10 minutes at 15-25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 1 hour after preparation.

PROGRAM SETUP
Program Name: TP Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,2200Note 1
Main Filter: 546 nm Normal Low: 6,6000 g/dL
Sub Filter: None nm Normal High: 8,3000 g/dL
Program Unit: g/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 g/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 g/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 g/dL
Linearity Min: 0,0080 g/dL Control P min: Enter valueNote 3 g/dL
Linearity Max: 15,0000 g/dL Control P max: Enter valueNote 3 g/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,008 g/dL (detection limit) to 15 g/dL (linearity limit). If the obtained results are greater than 15 g/dL, dilute the sample
1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

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Application sheet

REF HB020 Urine Total Protein

VOL 2 x 125 mL
Standard 1 x 5 mL
Pyrogallol-Red. Colorimetric.

REAGENT PREPARATION AND STABILITY

The reagents are ready for use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if the
reagent blanc absorbance is out of range, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Note 2
Control sera are recommended to monitor the performance of assay procedures. Each laboratory should establish its own QC scheme and
corrective actions if controls do not meet the acceptable tolerances.
SAMPLES
- Urine 24h: Stability 8 days at 2-8 °C.
- Cerebrospinal Fluid (CSF): Stable 4 days at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL Reagent 1
For StandardNote 2 20 µL Standard + 1 mL Reagent 1
For Sample 20 µL Sample + 1 mL Reagent 1
You can prepare several samples simultaneously. Mix and incubate for 5 minutes at 37 °C or for 10 minutes at 15-25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 30 minutes after preparation.

PROGRAM SETUP
Program Name: TPU Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,7000Note 1
Main Filter: 578 nm Normal Low: 0,0000Note 3 mg/dL
Sub Filter: None nm Normal High: 10,0000Note 3 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: 0,0000 mg/dL
Dilution Factor: 1,0000 Control N max: 0,0000 mg/dL
Linearity Min: 0,9440 mg/dL Control P min: 0,0000 mg/dL
Linearity Max: 400,0000 mg/dL Control P max: 0,0000 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 3

MEASURING RANGE
This method is linear from 0,944 mg/dL (detection limit) to 400 mg/dL (linearity limit). If the obtained results are greater than 400 mg/dL, dilute the
sample 1:2 with NaCl 9 g/L, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial).
3. These values are for urine samples. For CSF samples, please check the insert for the values and sample preparation.

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Application sheet

REF HB021 Triglycerides Lyo

VOL 12 x 20 mL
Standard 1 x 5 mL Enzymatic - colorimetric test
GPO-POD

REAGENT PREPARATION AND STABILITY

Dissolve the contents of one bottle R2 Enzymes into the contents of one bottle R1 Buffer. Cap and mix gently to dissolve the contents. This working
reagent is stable 6 weeks at 2-8°C or 1 week at room temperature (15-25°C).
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8°C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 500-550 nm ≥ 0,14, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or heparinized or EDTA plasma. The stability of the sample: 5 days at 2-8°C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL Working reagent
For StandardNote 2 10 µL Standard + 1 mL Working reagent
For Sample 10 µL Sample + 1 mL Working reagent
You can prepare several samples simultaneously. Mix and incubate for 5 minutes at 37 °C or for 10 minutes at 15-25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 30 minutes after preparation.

PROGRAM SETUP
Program Name: TRIG Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,1400Note 1
Main Filter: 510 nm Normal Low: 35,0000 mg/dL
Sub Filter: None nm Normal High: 160,0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,0000 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 1200,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0 mg/dL (detection limit) to 1200 mg/dL (linearity limit). If the obtained results are greater than 1200 mg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

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Application sheet

REF HBL060 HBL060A HBL060M Triglycerides

VOL 2 x 125 mL 8 x 125 mL 8 x 30 mL
Standard 1 x 5 mL 4 x 5 mL - Enzymatic. Colorimetric.
GPO-POD

REAGENT PREPARATION AND STABILITY

The reagent and standard are ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 500-550 nm ≥ 0,23, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Note 2 Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma. The stability of the sample: 5 days at 2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL Reagent
For StandardNote 2 10 µL Standard + 1 mL Reagent
For Sample 10 µL Sample + 1 mL Reagent
You can prepare several samples simultaneously. Mix and incubate for 5 minutes at 37 °C or for 10 minutes at 15-25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 30 minutes after preparation.

PROGRAM SETUP
Program Name: TRIGLn Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,2300Note 1
Main Filter: 510 nm Normal Low: 35,0000 mg/dL
Sub Filter: None nm Normal High: 150,0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 1,0100 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 1000,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 1,01 mg/dL (detection limit) to 1000 mg/dL (linearity limit). If the obtained results are greater than 1000 mg/dL, dilute
the sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.

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Application sheet

REF HB029 Urea Lyo

VOL 2 x 125 mL
Standard 1 x 5 mL
Berthelot. Enzymatic. Colorimetric

REAGENT PREPARATION AND STABILITY

R2 and Standard are ready for use.
Dissolve the content of one bottle R3 Enzymes into one bottle R1 Buffer. Cap and mix gently to dissolve the contents.
The working solution (R1 + R3) is stable 4 weeks at 2-8 °C or 1 week at room temperature (15-25 °C).
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 580 nm ≥ 0,32, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Note 2 Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls Specific
(HBC01-S, HBC02-S). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
- Serum or heparinized plasma: do not use ammonium salts or fluoride as anticoagulants.
- Urine, diluted 1:50 with distilled water. Mix. Multiply results by 50 (dilution factor). Preserve urine samples at pH < 4. Urea is stable for 5 days at
2-8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL Working reagent (R1+R3)
For StandardNote 2 10 µL Standard + 1 mL Working reagent (R1+R3)
For Sample 10 µL Sample + 1 mL Working reagent (R1+R3)
Mix, incubate at 37 °C for 5 min or 10 min at room temperature (15-25 °C). Then add Reagent 2:
For Blank 1 mL Reagent 2
For Standard 1 mL Reagent 2
For Sample 1 mL Reagent 2
You can prepare several samples simultaneously. Mix and incubate for 5 minutes at 37 °C or for 10 minutes at 15-25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 30 minutes after preparation.

PROGRAM SETUP
Program Name: UREA Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,3200Note 1
Main Filter: 578 nm Normal Low: 15,0000Note 4 mg/dL
Sub Filter: None nm Normal High: 45,0000Note 4 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,0010 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 225,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,001 mg/dL (detection limit) to 225 mg/dL (linearity limit). If the obtained results are greater than 225 mg/dL, dilute the
sample 1:2 with NaCl 9 g/L, repeat the determination, and multiply the result by factor 2.
NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator Specific (HBC03-S) for calibration. Program
NUM of STD (1) and CONC (as mentioned on the insert provided with HBC03-S).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum or heparinized plasma samples. For urine, please check the insert for the values and sample preparation.

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Application sheet

REF HBL03 Urea

VOL 160 + 40 mL
Standard 1 x 5 mL
Urease-GLDH. UV. Kinetic

REAGENT PREPARATION AND STABILITY

Working reagent (WR): Mix 4 volumes of R1 (Buffer) with 1 volume of R2 (Enzymes). After mixing, allow to stand for 30 minutes prior to use. The
stability of the working reagent is 1 month at 2-8 °C or 1 week at room temperature (15-25 °C). The standard is ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 340 nm < 1,00, the reagent should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or heparinized plasma: do not use ammonium salts or fluoride as anticoagulants. Urine: dilute the sample 1:50 with distilled water. Mix.
Multiply results by 50 (dilution factor). Preserve urine samples at pH < 4. Urea is stable for 5 days at 2 - 8 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
For Blank 1,00 mL Working reagent (R1 + R2)
For Pre-rinse 20 µL Standard + 2,00 mL Working reagent (R1 + R2)
For StandardNote 2 10 µL Standard + 1,00 mL Working reagent (R1 + R2)
For Sample 10 µL Sample + 1,00 mL Working reagent (R1 + R2)
Before calibration, rinse the instrument with the Pre-rinse mixture after 2 min incubation at 37 °C using the “Wash” button. Note 5
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working solution to
the sample/calibrator. Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks “Press PUSH Aspirate Sample”. Note 6
PROGRAM SETUP
Program Name: UREAL Blank Low: 1,0000Note 1
Program Method: Two Point Blank High: 2,5000Note 1
Main Filter: 340 nm Normal Low: 15,0000Note 4 mg/dL
Sub Filter: None nm Normal High: 45,0000Note 4 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 030 sec Factor: 0,0000Note 2
Test time: 060 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,7430 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 400,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,743 mg/dL (detection limit) to 400 mg/dL (linearity limit). If the obtained results are greater than 400 mg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.
NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that
deterioration of the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank
Low: 0,0000 and Blank High: 0,0100.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum or heparinized plasma samples. For urine, please check the insert for the values and sample preparation.
5. Prerinsing the flowcell with the Pre-rinse mixture is essential to improve the calibration accuracy.
6. Carry-over by pathological samples is possible. To prevent this, we recommend to follow a sample with concentration >50 mg/dL by aspiration
of 0,8 mL Reagent Blank (working solution) before proceeding with the next sample.

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Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

REF HBL030 HBL030M Urea

VOL 240 + 60 mL 6 x 30 + 3 x 15 mL
Standard 1 x 5 mL - Urease-GLDH. UV. Kinetic

REAGENT PREPARATION AND STABILITY

Working reagent: Mix 4 volumes of R1 (Buffer) with 1 volume of R2 (Substrate). After mixing, allow to stand for 30 minutes prior to use. The working
reagent can be stored at 2 – 8 °C or at room temperature (15 – 25 °C), and can be used as long as the blank absorbance is > 1,00 AU. The stability
of the working reagent is at least 24h at 15 - 25 °C. The standard is ready to use.
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2 - 8 °C.
Handle standard very carefully to prevent contamination. The reagent should be a clear solution. If turbidity or precipitation has occurred or if
blank absorbance at 340 nm ≤ 1,00 AU, the reagent should be discarded. Note 1
CALIBRATION & QUALITY CONTROL
For calibration, use the standard included in the kit. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
Serum or plasma: do not use ammonium salts or fluoride as anticoagulants. Stability of samples: 7 days at 4 - 25 °C.

PROCEDURE
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
For Blank 1,00 mL Working reagent (R1 + R2)
For Pre-rinse 20 µL Standard + 2,00 mL Working reagent (R1 + R2)
For StandardNote 2 10 µL Standard + 1,00 mL Working reagent (R1 + R2)
For Sample 10 µL Sample + 1,00 mL Working reagent (R1 + R2)
Before calibration, rinse the instrument with the Pre-rinse mixture after 2 min incubation at 37 °C using the “Wash” button. Note 5
Prepare, mix and measure one sample at a time. Aspirate the mixture in the instrument, immediately after addition of the working solution to
the sample/calibrator. Wait with the preparation of the next sample until the instrument is finished with measuring the previous one. At this time,
the instrument asks “Press PUSH Aspirate Sample”. Note 6

PROGRAM SETUP
Program Name: UREALn Blank Low: 1,0000Note 1
Program Method: Two Point Blank High: 2,5000Note 1
Main Filter: 340 nm Normal Low: 15,0000Note 4 mg/dL
Sub Filter: None nm Normal High: 45,0000Note 4 mg/dL
Program Unit: mg/dL Num of STD: 1Note 2
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 030 sec Factor: 0,0000Note 2
Test time: 060 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 1,6300 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 206,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: ReagentNote 1 Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 1,63 mg/dL (detection limit) to 206 mg/dL (linearity limit). If the obtained results are greater than 206 mg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.
NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag. Programming reagent as blank gives you the advantage that deterioration of
the reagent can be easily detected. Alternatively, you can program and aspirate distilled water as blank, with values for Blank Low: 0,0000 and Blank High:
0,0100.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD (1) and
CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum or heparinized plasma samples. For urine, please check the insert for the values and sample preparation.
5. Prerinsing the flowcell with the Pre-rinse mixture is essential to improve the calibration accuracy.
6. Carry-over by pathological samples is possible. To prevent this, we recommend to follow a sample with concentration >50 mg/dL by aspiration of 0,8 mL
Reagent Blank (working solution) before proceeding with the next sample.
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Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

REF HBL020 HBL020A HBL020M Uric Acid

VOL 2 x 125 mL 8 x 125 mL 8 x 30 mL
Standard 1 x 5 mL 4 x 5 mL - Enzymatic. Colorimetric
URICASE-POD

REAGENT PREPARATION AND STABILITY

Mix equal volumes of R1 Buffer and R2 Enzymes. This working reagent is stable for 2 months at 2-8 °C or 2 weeks at room temperature (15-25 °C).
All the components of the kit are stable up to the date of expiration as specified on the label, when stored tightly closed, protected from light and
contaminations prevented during their use. Storage temperature for this kit is 2-8 °C.
Handle standard very carefully to prevent contamination. The reagents should be clear solutions. If turbidity or precipitation has occurred or if
blank absorbance of the working reagent at 510 nm ≥ 0,12, the reagents should be discarded. Note 1

CALIBRATION & QUALITY CONTROL

For calibration, use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Note 2
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological Controls (HBC01,
HBC02). Note 3 Prepare and measure these controls the same as samples.
If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish
its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

SAMPLES
- Serum or plasma: stability 3-5 days at 2-8 °C or 6 months at -20 °C.
- Urine (24h): stability 4 days at 15-25 °C, pH > 8. Dilute sample 1:50 in distilled water. Mix. Multiply results by 50 (dilution factor). If urine is cloudy,
warm the specimen to 60 °C for 10 minutes to dissolve precipitated urates and uric acid. Do not refrigerate.

PROCEDURE
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
For Blank 1 mL Working reagent (R1 + R2)
For StandardNote 2 25 µL Standard + 1 mL Working reagent (R1 + R2)
For Sample 25 µL Sample + 1 mL Working reagent (R1 + R2)
You can prepare several samples simultaneously. Mix and incubate for 5 minutes at 37 °C or for 10 minutes at 15-25 °C (room temperature). After
the incubation time, aspirate and measure the samples within 45 minutes after preparation.

PROGRAM SETUP
Program Name: UAL Blank Low: 0,0000Note 1
Program Method: End Point Blank High: 0,1200Note 1
Main Filter: 510 nm Normal Low: 2,5000Note 4 mg/dL
Sub Filter: None nm Normal High: 7,7000Note 4 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration volume: 0800 µL CONC: value: see vialNote 2 mg/dL
Delay Time: 001 sec Factor: 0,0000
Test time: 003 sec Control N min: Enter valueNote 3 mg/dL
Dilution Factor: 1,0000 Control N max: Enter valueNote 3 mg/dL
Linearity Min: 0,1500 mg/dL Control P min: Enter valueNote 3 mg/dL
Linearity Max: 25,0000 mg/dL Control P max: Enter valueNote 3 mg/dL
Blank: Reagent Cuvette Temp: 37 °C
Num of Blank: 1

MEASURING RANGE
This method is linear from 0,15 mg/dL (detection limit) to 25 mg/dL (linearity limit). If the obtained results are greater than 25 mg/dL, dilute the
sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

NOTES
1. If the blank absorbance is out of range, the instrument will give you a flag.
2. Program the value of the standard (see vial). Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD
(1) and CONC (as mentioned on the insert provided with HBC03).
3. The control values can be found on the control sheets, delivered together with the control vials.
4. These values are for serum or plasma samples. For urine, please check the insert for the values and sample preparation.

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Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

LIN340

Once every year, CYANSmart should be validated to assure correct results.

Validation of CYANSmart is based on two principles:

• Verification of linearity
• Verification of precision

This is done by measuring two reference solutions at different wavelengths:

• Potassium dichromate at 340 nm using the LIN340 method
• Ammonium cobalt(II) sulfate hexahydrate at 510 nm using the LIN510 method

REAGENT PREPARATION AND STABILITY

Prepare a dilution series of Potassium Dichromate K2Cr2O7 as measuring solution.
See service manual for detailed procedure.

PROGRAM SETUP
Program Name: LIN340 Blank Low: 0,0000
Program Method: End Point Blank High: 0,0100
Main Filter: 340 nm Normal Low: 0,2000 g/L
Sub Filter: None nm Normal High: 1,7000 g/L
Program Unit: g/L Num of STD: 0
Aspiration volume: 0800 µL CONC: 0,0000 g/L
Delay Time: 001 sec Factor: 1,0000
Test time: 003 sec Control N min: 0,0000 g/L
Dilution Factor: 1,0000 Control N max: 0,0000 g/L
Linearity Min: 0,0050 g/dL Control P min: 0,0000 g/L
Linearity Max: 2,5000 g/dL Control P max: 0,0000 g/L
Blank: Water Cuvette Temp: 37 °C
Num of Blank: 1

2021-02 (4.0) - Replaces all previous versions

46/49
Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009 CYANSmart
Application sheet

LIN510

Once every year, CYANSmart should be validated to assure correct results.

Validation of CYANSmart is based on two principles:

• Verification of linearity
• Verification of precision

This is done by measuring two reference solutions at different wavelengths:

• Potassium dichromate at 340 nm using the LIN340 method
• Ammonium cobalt(II) sulfate hexahydrate at 510 nm using the LIN510 method

REAGENT PREPARATION AND STABILITY

Prepare a dilution series of Ammonium Cobalt(II) Sulfate Hexahydrate as measuring solution.
See service manual for detailed procedure.

PROGRAM SETUP
Program Name: LIN510 Blank Low: 0,0000
Program Method: End Point Blank High: 0,0100
Main Filter: 510 nm Normal Low: 0,3000 g/L
Sub Filter: None nm Normal High: 1,9000 g/L
Program Unit: g/L Num of STD: 0
Aspiration volume: 0500 µL CONC: 0,0000 g/L
Delay Time: 001 sec Factor: 1,0000
Test time: 003 sec Control N min: 0,0000 g/L
Dilution Factor: 1,0000 Control N max: 0,0000 g/L
Linearity Min: 0,0050 g/dL Control P min: 0,0000 g/L
Linearity Max: 2,5000 g/dL Control P max: 0,0000 g/L
Blank: Water Cuvette Temp: 37 °C
Num of Blank: 1

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Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
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 CY009

English
To all Healthcare Professionals,

Thank you for your interest. We appreciate it!

CYANSmart
Cypress Diagnostics provides the tools and solutions that
let clinical laboratories worldwide deliver clear and precise
diagnostic results.
We manufacture the equipment needed for handling the

Semi-Automatic Biochemistry
samples, analyzers to perform the tests, reagents to carry out
the tests and - increasingly often - the software platforms to
report the results.

Analyzer Cypress Diagnostics supplies clinical laboratories.

We are a quality-oriented company with ISO certification

and CE-approved products. Our production facility is located
in Hulshout, Belgium. Our products are preferred and
appreciated by users in more than 100 countries worldwide.
Cypress Diagnostics is a family-owned business, founded in
1995 in Leuven, Belgium.

We work exclusively with selected and trained distributors.

We sell high-quality instruments and customer service is an
integral part of our quality policy.
All our products come with an aftersales and performance
guarantee.

Please feel free to contact our local distributor for more

information.
Our latest inserts and catalogs are downloadable on
www.diagnostics.be .

Test the difference!

Kind regards,

The Cypress Diagnostics Team

ISO 13485-2016 53/54

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
CYANSmart Technical Specifications

Optical system Power supply

The CYANSmart is an an easy-to-operate semi-automatic biochemistry analyzer. This new in vitro diagnostic • Flow cell: 32 µl, 10 mm light path • AC 110/220 V - 50/60 Hz
instrument is designed to have powerful features in a compact, independent unit. • Min. reaction volume: 500 µl per test • Automatic voltage and frequency switch
• Halogen lamp 6V/10W • Grounding required
• Photo detector: Silicon based (range 300 – 900 nm) • Power: 200 VA, reduced to 140 VA in standby mode
Cost savings: Convenience:
• Measurement range: 0,000 – 3,500 Abs • Power save / Standby mode
• Minimum reaction volume of 500 µl means decreased • Easy tracking of all the results from each patient by a
• Wavelength: 340 – 620 nm
costs per test unique identifier
• Wavelength selection: Automatic via 7 interference Software
• Limited consumables required • All Cypress Diagnostics biochemistry reagents are
filters: 340, 405, 492, 510, 546, 578 & 620 nm • Unique 16 digit patient ID
• Extra items included on delivery (two fuses, five paper preprogrammed on delivery
• Memory: 180 test methods and 6000 sample test results
rolls, a spare lamp, and pump tube) • 180 open channels are programmable to allow for
Flow cell • Cypress Diagnostics methods preprogrammed on
• Integrated incubator, printer, pump, and flow cell other user-defined tests
• 25 °C, 30 °C, 37 °C, ambient temperature delivery
• Long life lamp, further extended by power saving / • Memory for up to 6000 sample results
• Peltier element • Languages: English, French, Spanish
standby mode • Large font size, easily legible and read with little effort
• Thermostatic control: PID controlled, ±0,5 °C • QC: 2 controls programmable plus a separate QC results
• Robustness of the analyzer with minimal daily • LCD touch screen with virtual alphanumeric keyboard
• 10 mm menu with (printable) graphs
maintenance • Multilingual capability
• Calculation methods: absorbance, endpoint, two-point,
• Analyzer guides the user and so reduces mistakes • Minimal daily maintenance
Incubator kinetic, bichromatic
• 20 places with 14 mm diameter • Calibration methods: factor, calibrator and multi-point
Flexibility:
• 37 °C calibration
• On-board printer for printing automatically or on Desktop Software:
• Thermostatic control: PID controlled, ± 1 °C • Blank options: sample blank, reagent blank and water
request printing • Clear reporting with patient overview with flags and
• Incubation time: 0 – 999 seconds
• Seven wavelengths (340, 405, 492, 510, 546, 578, and reference ranges
Display • Reading time: 3 – 999 seconds
620 nm filters) cover all clinical diagnostic applications • Easier reporting and result sharing (email & network
• Back-illuminated LCD
• Five calculation methods (absorbance, endpoint, printing)
• Touchscreen calibration possible Weight and dimensions
two-point, kinetic, bichromatic) and three calibration • Elimination of transcription errors (no more manual
• 800 x 480 pixels (screen size: 7 inch) • Instrument: 9 kg, 20 x 43,5 x 41,5 cm (H x W x L)
methods (factor, calibrator, and multi-point data entry)
calibration) provide plenty processing options with • Data back-up
Printer Environmental requirements
one system • Convenient upgrade of the methods by quick upload
• Automatic or on-demand printing • Ambient temperature: 15 °C – 30 °C
• Three blank options (sample blank, reagent blank, and • Built-in thermal printer • Relative humidity: 30 % - 70 %
water) provide background correction of the reagent • 24 characters per line
and sample coloration • Prints graphs Order code:
• CY009: CYANSmart

Contact: e-mail: [email protected] Your Distributor

tel: + 32 (0)15 67 67 68
Nijverheidsstraat 8
2235 Hulshout
Belgium
URL: www.diagnostics.be CYANSmart EN 2019-04

ISO 13485-2016 ISO 13485-2016 54/54

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium
www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: [email protected] www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]