EA50 Cytoplasmic stain

Safety Data Sheet dated 7/3/2019, version 3

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1. Product identifier
Mixture identification:
Trade name: EA50 Cytoplasmic stain
trade code: CP702-3-5-6
1.2. Relevant identified uses of the substance or mixture and uses advised against
Recommended use:
Reagent for Anatomy Pathology
Uses advised against:
not any
1.3. Details of the supplier of the safety data sheet
Company:
DIAPATH SpA - Via Savoldini , 71 - 24057 Martinengo (BG) -ITALY
DIAPATH SpA - phone +(39) 0363/986411 (8.30-17.30)
Competent person responsible for the safety data sheet:
[email protected]
1.4. Emergency telephone number
Centro Antiveleni - Ospedale di Niguarda - Milano - Tel. +39 02/66101029
Centro Antiveleni - Osp. Maggiore - Unità operativa di Tossicologia - Bologna - Tel. +39
051/382984235
Centro Antiveleni - Università di Roma, Policlinico Umberto I - Tel.+39 06/490663
Centro Antiveleno - Università di Torino - Tel. +39 011/637637
Centro Antiveleni - Ospedali Riuniti Cardarelli (c/o Usl 40) - Napoli - Tel. +39 081/5453333
Centro Antiveleni - Unità degli Studi di Messina (c/o Facoltà di Farmacia) - Tel. 090/6764059
Centro Antiveleni - Centro di Rianimazione e Terapia Intensiva, Osp. Generale Regionale
Centro Antiveleni - Istituto per l'Infanzia - Trieste - Tel. +39 040/3785373

SECTION 2: Hazards identification

2.1. Classification of the substance or mixture
EC regulation criteria 1272/2008 (CLP):
Flam. Liq. 2, H225 Highly flammable liquid and vapour.
Skin Irrit. 2, H315 Causes skin irritation.
Eye Irrit. 2, H319 Causes serious eye irritation.
Adverse physicochemical, human health and environmental effects:
No other hazards
2.2. Label elements
Hazard pictograms:

Danger
Hazard statements:
H225 Highly flammable liquid and vapour.
H315 Causes skin irritation.
H319 Causes serious eye irritation.
Precautionary statements:
P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No

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smoking.
P233 Keep container tightly closed.
P264 Wash ... Thoroughly after handling.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P370+P378 In case of fire: Use ... to extinguish.
P403+P235 Store in a well-ventilated place. Keep cool.
Special Provisions:
None
Special provisions according to Annex XVII of REACH and subsequent amendments:
None

2.3. Other hazards

vPvB Substances: None - PBT Substances: None
Other Hazards:
No other hazards

SECTION 3: Composition/information on ingredients

3.1. Substances
N.A.
3.2. Mixtures
Hazardous components within the meaning of the CLP regulation and related classification:
>= 80% - < 90% ethanol; ethyl alcohol
Index number: 603-002-00-5, CAS: 64-17-5, EC: 200-578-6
Flam. Liq. 2 H225 Highly flammable liquid and vapour.
Eye Irrit. 2 H319 Causes serious eye irritation.

>= 1% - < 3% acetic acid … %

Index number: 607-002-00-6, CAS: 64-19-7, EC: 200-580-7
Flam. Liq. 3 H226 Flammable liquid and vapour.
Skin Corr. 1A H314 Causes severe skin burns and eye damage.

>= 1% - < 3% methanol

Index number: 603-001-00-X, CAS: 67-56-1, EC: 200-659-6
Flam. Liq. 2 H225 Highly flammable liquid and vapour.
Acute Tox. 3 H311 Toxic in contact with skin.
Acute Tox. 3 H331 Toxic if inhaled.
Acute Tox. 3 H301 Toxic if swallowed.
STOT SE 1 H370 Causes damage to organs.

Specific Concentration Limits:

3% <= C < 10%: STOT SE 2 H371
C >= 10%: STOT SE 1 H370

>= 1% - < 3% propan-2-ol; isopropyl alcohol; isopropanol

Index number: 603-117-00-0, CAS: 67-63-0, EC: 200-661-7
Flam. Liq. 2 H225 Highly flammable liquid and vapour.
Eye Irrit. 2 H319 Causes serious eye irritation.
STOT SE 3 H336 May cause drowsiness or dizziness.

SECTION 4: First aid measures

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4.1. Description of first aid measures

In case of skin contact:
Immediately take off all contaminated clothing.
Areas of the body that have - or are only even suspected of having - come into contact with the
product must be rinsed immediately with plenty of running water and possibly with soap.
OBTAIN IMMEDIATE MEDICAL ATTENTION.
Wash thoroughly the body (shower or bath).
Remove contaminated clothing immediately and dispose off safely.
After contact with skin, wash immediately with soap and plenty of water.
In case of eyes contact:
After contact with the eyes, rinse with water with the eyelids open for a sufficient length of time,
then consult an opthalmologist immediately.
Protect uninjured eye.
In case of Ingestion:
Do not under any circumstances induce vomiting. OBTAIN A MEDICAL EXAMINATION
IMMEDIATELY.
In case of Inhalation:
Remove casualty to fresh air and keep warm and at rest.
4.2. Most important symptoms and effects, both acute and delayed
None
4.3. Indication of any immediate medical attention and special treatment needed
In case of accident or unwellness, seek medical advice immediately (show directions for use or
safety data sheet if possible).
Treatment:
None

SECTION 5: Firefighting measures

5.1. Extinguishing media
Suitable extinguishing media:
In case of fire: Use ... to extinguish.
Extinguishing media which must not be used for safety reasons:
None in particular.
5.2. Special hazards arising from the substance or mixture
Do not inhale explosion and combustion gases.
Burning produces heavy smoke.
5.3. Advice for firefighters
Use suitable breathing apparatus .
Collect contaminated fire extinguishing water separately. This must not be discharged into
drains.
Move undamaged containers from immediate hazard area if it can be done safely.

SECTION 6: Accidental release measures

6.1. Personal precautions, protective equipment and emergency procedures
For non emergency personnel:
Wear personal protection equipment.
Remove all sources of ignition.
Remove persons to safety.
See protective measures under point 7 and 8.
For emergency responders:
Wear personal protection equipment.
6.2. Environmental precautions
Do not allow to enter into soil/subsoil. Do not allow to enter into surface water or drains.
Retain contaminated washing water and dispose it.

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In case of gas escape or of entry into waterways, soil or drains, inform the responsible
authorities.
Suitable material for taking up: absorbing material, organic, sand
6.3. Methods and material for containment and cleaning up
Wash with plenty of water.
6.4. Reference to other sections
See also section 8 and 13

SECTION 7: Handling and storage

7.1. Precautions for safe handling
Avoid contact with skin and eyes, inhalation of vapours and mists.
Don't use empty container before they have been cleaned.
Before making transfer operations, assure that there aren't any incompatible material residuals
in the containers.
Contamined clothing should be changed before entering eating areas.
Do not eat or drink while working.
See also section 8 for recommended protective equipment.
7.2. Conditions for safe storage, including any incompatibilities
Always keep in a well ventilated place.
Keep away from unguarded flame, sparks, and heat sources. Avoid direct exposure to sunlight.
Keep away from food, drink and feed.
Incompatible materials:
None in particular.
Instructions as regards storage premises:
Cool and adequately ventilated.
7.3. Specific end use(s)
None in particular

SECTION 8: Exposure controls/personal protection

8.1. Control parameters
ethanol; ethyl alcohol - CAS: 64-17-5
TLV/STEL - TWA: 1880 mg/m3, 1000 ppm
ACGIH - STEL: 1000 ppm - Notes: A3 - URT irr
acetic acid … % - CAS: 64-19-7
TLV/TWA - TWA: 25 mg/m3, 10 ppm
EU - TWA(8h): 25 mg/m3, 10 ppm - STEL: 50 mg/m3, 20 ppm
ACGIH - TWA(8h): 10 ppm - STEL: 15 ppm - Notes: URT and eye irr, pulm func
methanol - CAS: 67-56-1
TLV-ACGIH - TWA(8h): 200 ppm - STEL(15min): 250 ppm
OEL - TWA(8h): 260 mg/m3, 200 ppm
EU - TWA(8h): 260 mg/m3, 200 ppm - Notes: Skin
ACGIH - TWA(8h): 200 ppm - STEL: 250 ppm - Notes: Skin, BEI - Headache, eye dam,
dizziness, nausea
propan-2-ol; isopropyl alcohol; isopropanol - CAS: 67-63-0
ACGIH - TWA(8h): 200 ppm - STEL: 400 ppm - Notes: A4, BEI - Eye and URT irr, CNS
impair
DNEL Exposure Limit Values
ethanol; ethyl alcohol - CAS: 64-17-5
Worker Industry: 1900 mg/m3 - Exposure: Human Inhalation - Frequency: Short Term,
local effects
Worker Industry: 950 mg/m3 - Exposure: Human Inhalation - Frequency: Long Term,
systemic effects
Worker Industry: 343 mg/kg - Exposure: Human Dermal - Frequency: Long Term,

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systemic effects
acetic acid … % - CAS: 64-19-7
Worker Industry: 25 mg/m3 - Exposure: Human Inhalation - Frequency: Short Term,
systemic effects
Worker Industry: 25 mg/m3 - Exposure: Human Inhalation - Frequency: Long Term,
systemic effects
Consumer: 25 mg/m3 - Exposure: Human Inhalation - Frequency: Short Term, systemic
effects
Consumer: 25 mg/m3 - Exposure: Human Inhalation - Frequency: Long Term, systemic
effects
propan-2-ol; isopropyl alcohol; isopropanol - CAS: 67-63-0
Consumer: 26 mg/kg - Exposure: Human Oral
Worker Industry: 888 mg/kg - Consumer: 319 mg/kg - Exposure: Human Dermal
Worker Industry: 500 mg/m3 - Consumer: 89 mg/m3 - Exposure: Human Inhalation
PNEC Exposure Limit Values
ethanol; ethyl alcohol - CAS: 64-17-5
Target: Fresh Water - Value: 0.96 mg/l
Target: Marine water - Value: 0.79 mg/l
Target: Freshwater sediments - Value: 3.6 mg/kg
Target: Marine water sediments - Value: 2.9 mg/kg
Target: Soil (agricultural) - Value: 0.63 mg/kg
acetic acid … % - CAS: 64-19-7
Target: Marine water sediments - Value: 1.136 mg/kg
Target: soil - Value: 0.47 mg/kg
Target: Fresh Water - Value: 3.058 mg/l
Target: Marine water - Value: 0.3058 mg/l
Target: Freshwater sediments - Value: 11.36 mg/kg
Target: occasional issue - Value: 30.58 mg/l
8.2. Exposure controls
Eye protection:
Use close fitting safety goggles, don't use eye lens.
Protection for skin:
Use clothing that provides comprehensive protection to the skin, e.g. cotton, rubber, PVC or
viton.
Protection for hands:
Use protective gloves that provides comprehensive protection, e.g. P.V.C., neoprene or rubber.
Respiratory protection:
Not needed for normal use.
Thermal Hazards:
None
Environmental exposure controls:
None
Appropriate engineering controls:
None

SECTION 9: Physical and chemical properties

9.1. Information on basic physical and chemical properties

Properties Value Method: Notes:

Appearance and colour: liquid green- -- --

pink

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Odour: characteristic -- --

Odour threshold: N.A. -- --

pH: 5.00 -- --

Melting point / freezing N.A. -- --

point:

Initial boiling point and N.A. -- --

boiling range:

Flash point: 17 ° C -- --

Evaporation rate: N.A. -- --

Solid/gas flammability: N.A. -- --

Upper/lower flammability N.A. -- --

or explosive limits:

Vapour pressure: N.A. -- --

Vapour density: N.A. -- --

Relative density: N.A. -- --

Solubility in water: N.A. -- --

Solubility in oil: N.A. -- --

Partition coefficient (n- N.A. -- --

octanol/water):

Auto-ignition temperature: N.A. -- --

Decomposition N.A. -- --
temperature:

Viscosity: N.A. -- --

Explosive properties: N.A. -- --

Oxidizing properties: N.A. -- --

9.2. Other information

Properties Value Method: Notes:

Miscibility: N.A. -- --

Fat Solubility: N.A. -- --

Conductivity: N.A. -- --

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Substance Groups N.A. -- --

relevant properties

SECTION 10: Stability and reactivity

10.1. Reactivity
Stable under normal conditions
10.2. Chemical stability
Stable under normal conditions
10.3. Possibility of hazardous reactions
It may generate flammable gases on contact with elementary metals (alkalis and alkaline earth),
nitrides, and powerful reducing agents.
It may catch fire on contact with oxidising mineral acids, elementary metals (alkalis and alkaline
earth), nitrides, organic peroxides and hydroperoxides, oxidising agents, and reducing agents.
10.4. Conditions to avoid
Stable under normal conditions.
10.5. Incompatible materials
Avoid contact with combustible materials. The product could catch fire.
10.6. Hazardous decomposition products
None.

SECTION 11: Toxicological information

11.1. Information on toxicological effects
Toxicological information of the product:
N.A.
Toxicological information of the main substances found in the product:
ethanol; ethyl alcohol - CAS: 64-17-5
a) acute toxicity:
Test: LD50 - Route: Oral - Species: Rat > 6200 mg/kg
Test: LC50 - Route: Inhalation - Species: Rat > 50 mg/m3
Test: LD50 - Route: Skin - Species: Rabbit = 20 g/kg
acetic acid … % - CAS: 64-19-7
a) acute toxicity:
Test: LC50 - Route: Inhalation - Species: Rat > 16000 Ppm - Duration: 4h
Test: LD50 - Route: Oral - Species: Rat = 3530 mg/kg
Test: LD50 - Route: Oral - Species: Mouse = 4960 mg/kg
b) skin corrosion/irritation:
Test: Skin Corrosive - Route: Skin - Species: Rat Positive
c) serious eye damage/irritation:
Test: Eye Corrosive - Route: irritation - Species: Rabbit Positive
methanol - CAS: 67-56-1
a) acute toxicity:
Test: LC50 - Route: Inhalation - Species: Rat = 83.2 mg/l - Duration: 4h
Test: LD50 - Route: Oral - Species: Rat = 5300 mg/kg
Test: LD50 - Route: Skin - Species: Rabbit = 15800 mg/kg
propan-2-ol; isopropyl alcohol; isopropanol - CAS: 67-63-0
a) acute toxicity:
Test: LC50 - Route: Inhalation - Species: Rat > 10000 Ppm - Duration: 6h
Test: LD50 - Route: Oral - Species: Rat = 5840 mg/kg
Test: LD50 - Route: Skin - Species: Rat = 12800 mg/kg
If not differently specified, the information required in Regulation (EU)2015/830 listed below must be
considered as N.A.:
a) acute toxicity;

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b) skin corrosion/irritation;
c) serious eye damage/irritation;
d) respiratory or skin sensitisation;
e) germ cell mutagenicity;
f) carcinogenicity;
g) reproductive toxicity;
h) STOT-single exposure;
i) STOT-repeated exposure;
j) aspiration hazard.

SECTION 12: Ecological information

12.1. Toxicity
Adopt good working practices, so that the product is not released into the environment.
ethanol; ethyl alcohol - CAS: 64-17-5
a) Aquatic acute toxicity:
Endpoint: EC10 - Species: chlorella vulgaris = 11.5 mg/l - Duration h: 72
Endpoint: EC10 - Species: selenastrum capricornutum = 0.44 g/l - Duration h: 72
Endpoint: NOEC - Species: chlamydomonas eugametos = 7.9 g/l - Duration h: 48
Endpoint: NOEC - Species: daphnia magna > 10 mg/l
Endpoint: NOEC - Species: ceriodaphnia dubia = 9.6 mg/l
Endpoint: EC50 - Species: artemia salina = 23.9 g/l - Duration h: 24
Endpoint: EC50 - Species: artemia salina nauplii = 857 mg/l - Duration h: 48
Endpoint: LC50 - Species: salmo gairdneri = 13 g/l - Duration h: 96
Endpoint: LC50 - Species: pimephales promelas > 13.5 g/l
acetic acid … % - CAS: 64-19-7
a) Aquatic acute toxicity:
Endpoint: LC50 - Species: oncorhynchus mykiss > 300.82 mg/l - Duration h: 96
Endpoint: EC50 - Species: daphnia magna > 300.82 mg/l - Duration h: 48
Endpoint: EC50 - Species: Algae > 300.82 mg/l - Duration h: 72
propan-2-ol; isopropyl alcohol; isopropanol - CAS: 67-63-0
a) Aquatic acute toxicity:
Endpoint: EC50 - Species: daphnia magna > 10000 mg/l - Duration h: 24
12.2. Persistence and degradability
None
ethanol; ethyl alcohol - CAS: 64-17-5
Biodegradability: Readily biodegradable
acetic acid … % - CAS: 64-19-7
Biodegradability: Readily biodegradable
12.3. Bioaccumulative potential
ethanol; ethyl alcohol - CAS: 64-17-5
Bioaccumulation: little bioaccumulative
12.4. Mobility in soil
ethanol; ethyl alcohol - CAS: 64-17-5
Mobility in soil: evaporates quickly
acetic acid … % - CAS: 64-19-7
Mobility in soil: Mobile
12.5. Results of PBT and vPvB assessment
vPvB Substances: None - PBT Substances: None
12.6. Other adverse effects
None

SECTION 13: Disposal considerations

13.1. Waste treatment methods

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Recover, if possible. Send to authorised disposal plants or for incineration under controlled
conditions. In so doing, comply with the local and national regulations currently in force.

SECTION 14: Transport information

14.1. UN number
ADR-UN Number: 1993
IATA-UN Number: 1993
IMDG-UN Number: 1993
14.2. UN proper shipping name
ADR-Shipping Name: FLAMMABLE LIQUID, N.O.S. (ethanol; ethyl alcohol, acetic
acid … %)
IATA-Shipping Name: FLAMMABLE LIQUID, N.O.S. (ethanol; ethyl alcohol, acetic
acid … %)
IMDG-Shipping Name: FLAMMABLE LIQUID, N.O.S. (ethanol; ethyl alcohol, acetic
acid … %)
14.3. Transport hazard class(es)
ADR-Class: 3
ADR-Label: 3
ADR - Hazard identification number: 33
IATA-Class: 3
IATA-Label: 3
IMDG-Class: 3
IMDG-Class: 3 INFLAMMABLE LIQUID
14.4. Packing group
ADR-Packing Group: II
IATA-Packing group: II
IMDG-Packing group: II
14.5. Environmental hazards
ADR-Enviromental Pollutant: No
IMDG-Marine pollutant: No
14.6. Special precautions for user
ADR-Subsidiary risks: -
ADR-S.P.: 274 601 640C
ADR-Transport category (Tunnel restriction code): 2 (D/E)
IATA-Passenger Aircraft: 353
IATA-Subsidiary risks: -
IATA-Cargo Aircraft: 364
IATA-S.P.: A3
IATA-ERG: 3H
IMDG-EmS: F-E , S-E
IMDG-Subsidiary risks: -
IMDG-Stowage and handling: Category B
IMDG-Segregation: -
14.7. Transport in bulk according to Annex II of Marpol and the IBC Code
N.A.

SECTION 15: Regulatory information

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15.1. Safety, health and environmental regulations/legislation specific for the substance or mixture
Dir. 98/24/EC (Risks related to chemical agents at work)
Dir. 2000/39/EC (Occupational exposure limit values)
Regulation (EC) n. 1907/2006 (REACH)
Regulation (EC) n. 1272/2008 (CLP)
Regulation (EC) n. 790/2009 (ATP 1 CLP) and (EU) n. 758/2013
Regulation (EU) 2015/830
Regulation (EU) n. 286/2011 (ATP 2 CLP)
Regulation (EU) n. 618/2012 (ATP 3 CLP)
Regulation (EU) n. 487/2013 (ATP 4 CLP)
Regulation (EU) n. 944/2013 (ATP 5 CLP)
Regulation (EU) n. 605/2014 (ATP 6 CLP)
Regulation (EU) n. 2015/1221 (ATP 7 CLP)
Regulation (EU) n. 2016/918 (ATP 8 CLP)
Regulation (EU) n. 2016/1179 (ATP 9 CLP)
Regulation (EU) n. 2017/776 (ATP 10 CLP)
Restrictions related to the product or the substances contained according to Annex XVII Regulation
(EC) 1907/2006 (REACH) and subsequent modifications:
Restrictions related to the product:
Restriction 3
Restriction 40
Restrictions related to the substances contained:
Restriction 69
Where applicable, refer to the following regulatory provisions :
Directive 2012/18/EU (Seveso III)
Regulation (EC) nr 648/2004 (detergents).
Dir. 2004/42/EC (VOC directive)

Provisions related to directive EU 2012/18 (Seveso III):

Seveso III category according to Annex 1, part 1
Product belongs to category: P5c

15.2. Chemical safety assessment

No Chemical Safety Assessment has been carried out for the mixture.

SECTION 16: Other information

Text of phrases referred to under heading 3:
H371 May cause damage to organs.
H370 Causes damage to organs.

Hazard class and Code Description

hazard category

Flam. Liq. 2 2.6/2 Flammable liquid, Category 2

Flam. Liq. 3 2.6/3 Flammable liquid, Category 3

Acute Tox. 3 3.1/3/Dermal Acute toxicity (dermal), Category 3

Acute Tox. 3 3.1/3/Inhal Acute toxicity (inhalation), Category 3

Acute Tox. 3 3.1/3/Oral Acute toxicity (oral), Category 3

Skin Corr. 1A 3.2/1A Skin corrosion, Category 1A

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Skin Irrit. 2 3.2/2 Skin irritation, Category 2

Eye Irrit. 2 3.3/2 Eye irritation, Category 2

STOT SE 1 3.8/1 Specific target organ toxicity - single exposure,

Category 1

STOT SE 2 3.8/2 Specific target organ toxicity - single exposure,

Category 2

STOT SE 3 3.8/3 Specific target organ toxicity - single exposure,

Category 3

Paragraphs modified from the previous revision:

SECTION 9: Physical and chemical properties

Classification and procedure used to derive the classification for mixtures according to Regulation (EC)
1272/2008 [CLP]:

Classification according to Regulation (EC) Nr. Classification procedure

1272/2008

Flam. Liq. 2, H225 On basis of test data

Skin Irrit. 2, H315 Calculation method

Eye Irrit. 2, H319 Calculation method

This document was prepared by a competent person who has received appropriate training.
Main bibliographic sources:
ECDIN - Environmental Chemicals Data and Information Network - Joint Research Centre,
Commission of the European Communities
SAX's DANGEROUS PROPERTIES OF INDUSTRIAL MATERIALS - Eight Edition - Van
Nostrand Reinold
The information contained herein is based on our state of knowledge at the above-specified date. It
refers solely to the product indicated and constitutes no guarantee of particular quality.
It is the duty of the user to ensure that this information is appropriate and complete with respect to the
specific use intended.
This MSDS cancels and replaces any preceding release.

ADR: European Agreement concerning the International Carriage of

Dangerous Goods by Road.
ATE: Acute Toxicity Estimate
ATEmix: Acute toxicity Estimate (Mixtures)
CAS: Chemical Abstracts Service (division of the American Chemical
Society).
CLP: Classification, Labeling, Packaging.
DNEL: Derived No Effect Level.
EINECS: European Inventory of Existing Commercial Chemical Substances.
GefStoffVO: Ordinance on Hazardous Substances, Germany.
GHS: Globally Harmonized System of Classification and Labeling of

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Chemicals.
IATA: International Air Transport Association.
IATA-DGR: Dangerous Goods Regulation by the "International Air Transport
Association" (IATA).
ICAO: International Civil Aviation Organization.
ICAO-TI: Technical Instructions by the "International Civil Aviation Organization"
(ICAO).
IMDG: International Maritime Code for Dangerous Goods.
INCI: International Nomenclature of Cosmetic Ingredients.
KSt: Explosion coefficient.
LC50: Lethal concentration, for 50 percent of test population.
LD50: Lethal dose, for 50 percent of test population.
PNEC: Predicted No Effect Concentration.
RID: Regulation Concerning the International Transport of Dangerous Goods
by Rail.
STEL: Short Term Exposure limit.
STOT: Specific Target Organ Toxicity.
TLV: Threshold Limiting Value.
TWA: Time-weighted average
WGK: German Water Hazard Class.

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