INSTRUCTIONS MANUAL

CODE 80392 REV 0 DEC 2019 (It can be modified without notice)

SOFTWARE
M.WAVE PROFESSIONAL
V-1100 / VR-2000

USER MANUAL
(FOR SPECTROPHOTOMETERS
V-1100 & VR-2000)

4312001

J.P. SELECTA s.a.u. Autovía A-2 Km 585.1 Abrera 08630 (Barcelona) España Tel 34 937 700 877 Fax 34 937 702 362
e-mail: [email protected] - website: http://www.jpselecta.es
 Contents
Functions ............................................................................................... 1

Main Functions ............................................................................... 1

Spectrum Processing Function .............................................. 2

System Check and Calibration Function ......................... 3

Starting.................................................................................................. 3

PC System Requirements............................................................. 3

Install UV-Vis Analyst............................................................. 4

Uninstall UV-Vis Analyst ........................................................ 6

Run UV-Vis Analyst ...................................................................... 6

Setup Communication Port ........................................................ 7

Introduction ........................................................................................ 7

Main Interface ............................................................................... 7

Menus Bar and Tools Bar .......................................................... 8

Operation ............................................................................................. 13

Single Wavelength Photometric Measurement .............. 13

Fixed Point Measurement ........................................................ 14

Multi-wavelength Photometric Measurement ............ 14

Concentration Measurement................................................ 17

Wavelength Scanning .................................................................. 22

Time Scanning (Kinetic Analysis) ................................... 38

DNA/Protein Measurement ........................................................ 41

Appendix................................................................................................ 45

Methods of Quantitative Analysis ................................... 45

 UV-Vis Analyst

Functions
This section introduces the functions of the UV-Vis
Analyst.

Main Functions
Single wavelength photometric measurement
 Go to a desired wavelength quickly and conveniently.
 Photometric value display mode can be changed (%T or
Abs).

Fixed Points Measurement

Multi-wavelength Photometric Measurement
 Up to 20 wavelength points can be set up.
 Results will be grouped into a table format
automatically.

Concentration Measurement
 2 methods to set up the regression curve.
Up to 20 standards to set up the regression curve.
The UV-Vis Analyst will calculate the working curve
using a linear equation that fits the data. Enter
factor values to generate regression curve.
 3 methods for curve fit.
Linear fit, Quadratic fit and Cubic fit.

Wavelength Scanning
 Allow user to set scan step (0.1, 0.2, 0.5, 1.0 and
5.0nm).
 Spectrum display mode can be changed (Wavelength-%T
or Wavelength-Abs).
 Peaks and valleys will be automatically detected
after scanning (User can define the peak threshold).
 Powerful spectrum processing functions are provided.

Time Scanning

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 UV-Vis Analyst

 Allow user to set scan Interval (0.5, 1.0, 2.0, 5.0,

10, 30 and 60s).
 Spectrum display mode can be changed (Time-%T or
Time-Abs).
 Peaks and valleys will be automatically detected
after scanning (User can define the peak threshold).
 Powerful spectrum processing functions are provided.

DNA/Protein Measurement
 7 methods to measure DNA/Protein.
 Wavelength points and ratios can be set up.
 Results will be grouped into a table format
automatically.

Spectrum Processing Function

Trace a Spectrum
The cursor can be moved to a desired point in the
spectrum displayed on the screen and the photometric
data at this point is displayed.

Automatic Peak Detection

After a scanning is complete, peaks and valleys can be
automatically detected and listed in a table format.
They will also be labeled on the spectrum.

Scale Expansion
Simultaneous expansion of the X and Y axes are provided
with the “Zoom” function. Display range can also be
changed though the “Display Setup” function.

Differentiation
You can calculate and display the first through to the
fourth derivative spectrum for a given spectrum.
Derivative spectrum is useful for enhancing spectrum
data that are not readily apparent in an absorbance
spectrum.

Calculate Spectrum
You can calculate addition, subtraction, multiplication
and division between two spectrum with the resulting
data displayed on the screen.

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System Check and Calibration

Function
Instrument Validity Check
Up to 10 wavelength points can be set up in the
instrument validity mode. Two methods can be selected
(Photometric Validity measurement and Wavelength
Validity measurement) and tolerance can be entered.
Results will be grouped into a table format
automatically.

Dark Current Check

You can resample the dark current of the instrument.

Spectrum Bandwidth Check

A special scan for checking spectrum Bandwidth and it
will calculate the spectrum Bandwidth value
automatically.

Energy of Light Sources Check

It allows scan the energy of light sources with a fixed
gain (0-7).

Reset Wavelength
It affords to relocate the 656.1nm.

Starting
This section introduces how to start to use the UV-Vis
Analyst.

PC System Requirements
 Pentium or above PC;

 CD-ROM;

 USB Ports.
 512MB Memory(1GB or Above is strongly recommended);

 50MB or above hard disc Space;

 Microsoft Windows XP/Vista/7/8.

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 UV-Vis Analyst

Install UV-Vis Analyst

Note: Please disconnect the USB Cable and

unplug the USB Key before install the
UV-Vis Analyst software.

1. Put UV-Vis Analyst disc in the CD-ROM;

2. Double Click to open the CD-ROM, and then, double
click Setup.exe which is under the root directory of
CD to start installation , click Next;

3. Choose install path, then click Next to copy files

to PC;

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 UV-Vis Analyst

4. After finished copy all files of UV-Vis Analyst, it

will start install the USB Drive. Click Next;

< Back Next > Cancel

5. Select “I accept this agreement”. Click Next to copy

files to PC;

< Back Next > Cancel

6. Click Finish to finish install USB Drive;

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 UV-Vis Analyst

< Back Finish Cancel

7. Click Finish to finish all the setup.

Uninstall UV-Vis Analyst

Start→All Programs →UV-Vis Analyst→Uninstall UV-Vis
Analyst to remove.

Run UV-Vis Analyst

Note: Before you run UV-Vis Analyst, please
check these things as follow:

 Computer has connected to spectrophotometer by

using the USB cable;
 Spectrophotometer is on main interface.

WL: 500.0nm 10:00:00

D2
W

Main Menu
Basic Mode
Quantitative Mode
Wavelength Scan
Kinetics Mode
DNA/Protein Mode
Multi-Wavelength Mode
System Utility

Cancel Move Dn Select

There are two ways to start the UV-Vis Analyst:

 Double-click shortcut icon on the desktop.

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 UV-Vis Analyst

 Start→All Programs→UV-Vis Analyst→UV-Vis Analyst.

Setup Communication Port

On the UV-Photometer menu, click Comm. Hub Setup
appears the following box, select the RS232 Port and
Baud Rate (38400), click OK.

Introduction
We will introduce the UV-Vis Analyst in this chapter.

Main Interface
This is the main interface after start.

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 UV-Vis Analyst

Menu Bar Toolbar

Data Zone

Status Bar

Menus Bar and Tools Bar

Menu bar and Toolbar are both provided in the software
offering you two ways to select a desired function.
 On the menu bar, use your keypad or mouse to select
the desired function.
 Almost all the functions listed in the menu bar can
be reached by clicking a corresponding button in the
toolbar.

Main Menu Sub Menu Tool Function

New a Fixed

File New Points

New a

Measurement
Wavelength
New a Time Scan
Scan

Measurement
Measurement
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 UV-Vis Analyst

New a

DNA/Protein
New a

Measurement
Instrument
Open… Open a

Validity
spectrum/data
Close Close current

file
measurement
Save Save current

Save As… measurement

Save current

Open file from measurement

Open a file as

UV-Photometer a new file name

saved
Export Exportin
data or

instrument
method
Print… Print test

Print Setup… report

Setup printer

Exit Exit UV-Vis

Status Bar analyst

Display/Hide

Status of status
Displaybar
status
View
Spectrophotomet
Status font of
Setup font of

er spectrophotomet
status
Customize Define bar
the

er
information of

9 display and

print
 UV-Vis Analyst

Peaks Mark peak value

Valleys Mark valley

Magnify value
Magnify the

Restore area selected

Restore the

Search default
Search

parameters
peak/valley one
Link Connect to the

fdisplay
by one
Spectrophotomet
Reset Instrument
Reset

er
Spectrophotomet parameters of
Escape Stop current

er instrument
measurement
View dark Retest the dark

UV-
Current
Set Amplifier current
Reset amplifier
Photomete
Locate 656.1nm Relocate
r

Calibrate 656.1nm
Scan system

System Baseline
Automatic Blank baseline
Do blank

Calibration
Slit Bandwidth Set slit

*
Set Unit bandwidth
Set unit (0.5,

1.0, 2.0, 4.0)

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Turn on/off W Turn on/off W

lamp
Turn on/off D2 lamp
Turn on/off D2

lamp
D2/W Switch lamp
Set switch

Point
Comm. Port point
Setup of D2/W
comm.

Setup
Change Password port
Set/Change

Locate Cell ** login

Locatepassword
cell (1-
Auto-
Setup Multicell 8) to light
Setup Multicell
sample
** path
Autorun ** Measure multi

Start samples
Start a

automatically
measurement
Scan Stop Stop a

Service measurement
Measure

Display Range spectrum and

Setup scan
Settings
scan energy
display
Peak Height Define

parameters
peak/valley
Add Add two
Compute
threshold
spectrum
Sub Subtract one

spectrum from

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 UV-Vis Analyst

Multiply Multiply two

Divide spectrum
Divide one

Moving Window spectrum

Smooth a from

Averaging another
spectrum
Savitzky-Golay Smooth a with

Smoothing the method

spectrum with
Derivate Derivative of a

Filter Moving
the Window
method
Resample spectrum
Resample a
Averaging
Savitzky-Golay
New Window spectrum
New a
Smoothing
Cascade measurement
Multi windows
Filter
window
display as
in a
Window Tile Multi windows

current
cascade
Arrange Icons display
Arrange in
alla

tile
icons
Split Split minimized
display

Help About UV-Vis area

Display the

Analyst information
Setup

about the UV-

measurement
Modify a

Vis Analyst
parameters
measurement
Delete results

result
selected

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 UV-Vis Analyst

Set and Goto

one wavelength
Display

Instrument CPU
Delete current

information
Spectrum
Display result

as mode %T
Display result

as mode
Undo Abs
Scale

Operation
This chapter introduces how to use UV-Vis Analyst.

Single Wavelength Photometric

Measurement
The UV-Vis Analyst provides a convenient method to
measure photometric value at a fixed wavelength.

1. Click on the toolbar, appears Goto specified

wavelength.

2. Key in the desired wavelength position, click Goto.

The minimum wavelength step is 0.1nm in a range from

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 UV-Vis Analyst

190-1100nm.
3. Do blank.
 Single Beam: Place a reference in the
compartment, click Zero.
 Double Beam: Skip to step 4.
4. Measure Sample.
 Single Beam: Place a sample in the sample
compartment.
 Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.
The photometric value will display in the Readout
box.

Fixed Point Measurement

This UV-Vis Analyst performs fixed wavelength
measurement at 1-20 points and how to analyze unknown
compounds against calibration standards.

Multi-wavelength Photometric Measurement

1. Click on the toolbar, appears follow form.

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 UV-Vis Analyst

2. Click the Method tab.

3. Type the number of wavelength points in the Number
of WL Points box, or click the up/down arrows next
to the box set the wavelength points. Leave the two
boxes Calculate Concentration and Use Standard
Samples.
4. Key in the wavelength in the Wavelength box.
5. Click the Sample tab. It will display the following.
The control menu contains six buttons: Start,
Delete, Modify, Recalculate, Data Font and Print.

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 UV-Vis Analyst

6. Do blank.
 Single Beam: Place a reference in the

compartment, click .
 Double Beam: Skip to step 7.
7. Measure Sample.
 Single Beam: Place a sample in the sample
compartment.
 Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.

Click to run a new measurement. The display

will change to the following. Key in the sample name
in the Name box.

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 UV-Vis Analyst

8. Click OK. The photometric data for sample will be

listed in the Sample table.
9. Repeat steps 7-8 to measure all samples.

Concentration Measurement
Set Up Linear Regression Curve
There are two methods available to set up the linear
regression curve. You can use standards to set up the
regression curve or just key in the parameters
manually. Use the following steps to select the method
you wish to use.

1. Click on the toolbar.

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 UV-Vis Analyst

2. Click the Method tab.

3. Enter the number of wavelength points in the Number
of Points box.
4. Key in the wavelengths in the Wavelength boxes.
5. Tick the Calculate Concentration check box to
activate concentration calculation.
6. Set up the linear regression curve.
Method 1: Set up the linear regression curve with
prepared standards.
(1) Tick the Use Standard Samples check box.
(2) Click the Standard tab.
(3) Do blank.
 Single Beam: Place a reference in the

compartment, click .
 Double Beam: Skip to step (4).
(4) Measure Standard Sample.
 Single Beam: Place a sample in the sample
compartment.
 Double Beam: Place a reference in the reference
compartment and place a sample(Standard 1) in
the sample compartment.
Click Start to run a measurement.
(5) Key in the concentration value of Standard 1 in
the Conc. box.
(6) Key in the sample name for the standard in the
Name box.
(7) Click OK. The photometric data, △A and
concentration will be shown in the standard table.
(8) Repeat steps (4)-(7) to measure all the prepared
standards.

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 UV-Vis Analyst

(9) Click down arrow in Curve Fit box to select curve

fit method.

Method 2: Input the factor of the linear regression

curve.
(1) Leave the Use Standard Samples check box.
(2) Click down arrow in Curve Fit box to select curve
fit method.
(3) Input the factor of the linear regression curve.
7. Click Fitting tab to view the linear regression
curve. Click Display Setting tab to set the display
parameters and unit of concentration.

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Measure Concentration by Using The Linear Regression

Curve
The following procedure shows how to measure
concentration of samples.

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 UV-Vis Analyst

1. Set up linear regression curve or click to open

a file of linear regression curve (*.QUA).
2. Click the Sample tab.
3. Do blank.
 Single Beam: Place a reference in the

compartment, click .
 Double Beam: Skip to step 4.
4. Measure Sample.
 Single Beam: Place a sample in the sample
compartment.
 Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.

Click to run a new measurement. Type the sample

name in the Name box. The default is Sample-1.
5. Click OK. The photometric result for Sample-1 will
be listed in the sample data. Delta Abs. and
concentration value of Sample-1 will also be
displayed in columns 3 and 4.
6. Repeat steps 4-5 to measure remaining samples.

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Wavelength Scanning
This chapter describes how to collect a spectrum while
using Wavelength Scan function.

Scan Sample

1. Click on the toolbar to new a sample scan

measurement, appears the following form.

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 UV-Vis Analyst

2. Click on the toolbar, appears the following

form. Input start wavelength in From box (range:
190-1100nm), end wavelength in To box (range: 190-
1100nm), select scan interval (0.1, 0.2, 0.5, 1.0,
2.0 or 5.0nm) and Filter times (5, 10, 30 or 50),
click OK.

3. Click on the toolbar to select %Transmittance

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 UV-Vis Analyst

mode or click to select Absorbance mode.

4. Click on the toolbar to set display parameters.

5. Scan baseline.
 Single Beam: Place a reference in the

compartment, click .
 Double Beam: Skip to step 6.
6. Measure Sample.
 Single Beam: Place a sample in the sample
compartment.
 Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.

Click to scan sample, the real time spectrum

will be displayed. Click to cancel while

scanning.

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Auto List Peaks and Valleys

Click on the toolbar to set the peak/Valley

threshold (range: 0 to 1.000, step: 0.001), Input the

threshold value, click OK. Click to list peaks and

click to list valleys.

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Rescale

Click on the toolbar to set the new parameters for

display.

Original Scales

Click on the toolbar to restore the default display

settings.

Zoom Selected Area

Click on the toolbar to activate zoom function.

Position the cursor in the upper-left corner of the
area you want to select. Hold the left mouse button to
drag the cursor to outline the spectrum area you want
to enlarge. Release the mouse button. The part of the
spectrum which is displayed within the outlined area

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 UV-Vis Analyst

will be enlarged. Click to undo scale. To cancel

zoom to click again.

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 UV-Vis Analyst

Trace a Spectrum

Click on the toolbar, a crosshair cursor appears,

move the cursor on the spectrum. Move the crosshair
cursor left or right on the spectrum. The data in the
cursor window indicate the X-axis and Y-axis values for
the current cursor location. Click key “ESC” to release
the crosshair cursor.

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 UV-Vis Analyst

Select a Spectrum as Current

As UV-Vis Application Software can display several
spectrum overlaid on the screen, you should specify the
spectrum you wish to process. Click the down arrow on
the toolbar. All spectrums will be listed in the pull-
down menu. Click the spectrum you want to select. Its
name will be listed in the Name Box and it will be
referred to as Current Spectrum.

Derivative

Click on the toolbar. The following dialogue box

appears. Key in the class of derivative (1-10,
depending on whether 1st, 2nd, … 10th derivative is
required) and type a name for the result spectrum, then
click OK. The result spectrum will be displayed

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 UV-Vis Analyst

overlaid with the original one.

Moving Window Averaging

Click on the toolbar. Appears following form. Click

up/down arrow of the Range box to select range value,
key a file name in the Name box, click OK. The result
spectrum will be displayed overlaid with the original
one.

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 UV-Vis Analyst

Savitzky-Golay Smoothing Filter

On the Computer menu, click Savitzky-Golay Smoothing
Filter. Appears following form. Click up/down arrow to
select the parameters, key a file name in the Name of
Result box, click OK. The result spectrum will be
displayed overlaid with the original one.

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 UV-Vis Analyst

Resample

Click on the toolbar. The following dialogue box

will be displayed. Click Up/Down arrow to select Sample
times. Click OK. The new spectrum displays.

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 UV-Vis Analyst

Spectrum Addition
Spectrum addition can assist in the development of
artificial spectrum in multi-component mixtures.

Click on the toolbar. The following dialogue box

will be displayed. Click the down arrow next to File 1
to select a spectrum and define it as source 1. Select
a spectrum for File 2 in the same way. It will not
allow you to select the same spectrum twice. Key in a
name for the Result spectrum and click OK. The result
spectrum will be displayed on the screen.

Note:UV-Vis Analyst will only add, subtract,

multiply and divide two spectrums that are
already displayed on the screen. Before
arithmetic processing, load or collect two
spectrums from memory.

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 UV-Vis Analyst

Spectrum Subtraction
Subtracting one spectrum from another has been a
classical technique to offset spectrum interference
from the spectrum of interest.

Click on the toolbar. The following dialogue box

will be displayed. Click the down arrow next to File 1
to select a spectrum and define it as source 1. Select
a spectrum for File 2 in the same way. It will not
allow you to select the same spectrum twice. Key in a
name for the Result spectrum and click OK. The result
spectrum will be displayed on the screen.

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 UV-Vis Analyst

Spectrum Multiplication
Multiplying spectrum can assist in the development of
artificial structure of spectrum in multi-component
mixtures.

Click on the toolbar. The following dialogue box

will be displayed. Click the down arrow next to File 1
to select a spectrum and define it as source 1. Select
a spectrum for File 2 in the same way. It will not
allow you to select the same spectrum twice. Key in a
name for the Result spectrum and click OK. The result
spectrum will be displayed on the screen.

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 UV-Vis Analyst

Spectrum Division
Dividing one spectrum from another has been a classical
technique to offset spectrum interference from the
spectrum of interest.

Click on the toolbar. The following dialogue box

will be displayed. Click the down arrow next to File 1
to select a spectrum and define it as source 1. Select
a spectrum for File 2 in the same way. It will not
allow you to select the same spectrum twice. Key in a
name for the Result spectrum and click OK. The result
spectrum will be displayed on the screen.

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 UV-Vis Analyst

Unload a Spectrum
Select the spectrum you want to unload as the Current

Spectrum, Click on the toolbar to remove the

spectrum from the display.

Define Display Information

Click on the toolbar, appears the Settings to

display and print the spectra form, click the Legend
tab, type the information for display.

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Edit Print Information

Click on the toolbar, appears the Settings to

display and print the spectra form, click the Print
tab, type the information for print out.

Time Scanning (Kinetic Analysis)

This chapter tells you how to obtain the absorbance or
transmittance value for a sample as a function of time
at a given wavelength.

Scan Sample

1. Click on the toolbar, the following dialog box

will appear.

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 UV-Vis Analyst

2. Click on the toolbar to select the %transmittance

mode or click to select the absorbance mode.

3. Click on the toolbar. A dialogue box will be

displayed. Key in the wavelength, total time (in
seconds) and scan step in the above dialog box. The
wavelength range should be within 190 to 1100 nm. The
upper limit for total time is 100000 seconds. Seven
scan intervals can be selected from 0.5S, 1S, 2S, 5S,
10S, 30S and 60S. Click OK.

4. Do blank.
 Single Beam: Place a reference in the

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 UV-Vis Analyst

compartment, click .
 Double Beam: Skip to step 5.
5. Measure Sample.
 Single Beam: Place a sample in the sample
compartment.
 Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.

Click on the toolbar. The instrument will start

scanning automatically. The graph will be displayed on
the screen during time scanning. You can stop scanning

by clicking .

Calculate Rate

Click on the toolbar, appears the Settings to

display and print the spectra form, click the Dynamic
Analysis tab, type the begin time in Time Begin box,

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 UV-Vis Analyst

type the end time in Time End box, and type the K
factor in K Factor box, click Calculate, the result
will be displayed.

Define Display Information

Click on the toolbar, appears the Settings to

display and print the spectra form, click the Legend
tab, type the information for display.

Edit Print Information

Click on the toolbar, appears the Settings to

display and print the spectra form, click the Legend
tab, type the information for display.

DNA/Protein Measurement
This chapter describes how to perform DNA/Protein

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 UV-Vis Analyst

measurement.

DNA/Protein Measurement

1. Click on the toolbar, the following dialog box

will appear.

2. Click the down arrows of the method to select the test

method. Key in the wavelength position in the
Wavelength box. Key in the value of DNA/Protein Conc.
3. Click the Sample tab. It will display the following.
The control menu contains six buttons: Start, Delete,
Modify, Recalculate, Font and Print.

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 UV-Vis Analyst

4. Do blank.
 Single Beam: Place a reference in the

compartment, click .
 Double Beam: Skip to step 5.
5. Measure Sample.
 Single Beam: Place a sample in the sample
compartment.
 Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.

Click Start or to run a new measurement. The

display will change to the following.

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6. The UV-Vis Analyst will read the photometric value of

sample 1 at the fixed wavelength automatically. Key
in the sample name in the Name box. Click OK after
the measurement is complete. The photometric data for
sample 1 will be listed in the sample table.
7. Repeat steps 5-6 to test all samples.

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Appendix
Methods of Quantitative Analysis
Single Wavelength Method ：Abs.=A1
Double Wavelengths Method ：Abs.=m*A1-n*A2
Three Wavelengths Method ：Abs.=A1-(WL1-WL2)*(A2-
A3)/(WL2-WL3)-A3

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UV-VIS ANALYST FOR SPECTROPHOTOMETER