Water-Jacketed, IR Autoflow Automatic CO2 Incubator Models NU-2500E, 2600E, 2700E Operation and Maintenance Manual Revised November, 1990 ! Manufactured by: i NuAire, Inc. 2100 Fernbrook Lane Plymouth, Minnesota, USA, 55447 Tollfree 1-800-328-3352 In MN 612-553-1270Water-Jacketed, IR Autoflow Automatic CO2 Incubator Operation and Maintenance Manual TABLE OF CONTENTS Section No.1 .. - General Description Section No. 2 Performance Parameters Section No. 3 ‘Models and Features Section No. 4 1. Test Performance and Procedures Section No. 5 = Warranty Section No, 6 ee -Shipments Section NO. 7 eeeeeeerseerseees «Installation Section No.8 . “IR Autotiow Operation at. ves Sterility 82. +. Humidity as. NICE System Introduction a4. NICE System Front Control Panel 85. “IF Autoflow Rear Panel ..INIGE Run Mode Operator Interactions - NICE Setup Mode Operator Interac -NICE Diagnostic Interactions ... .Reload Default Configuration Bose |. .NICE Configuration interactions Section No. 9 Calibration/Adjustment a1. :. Chamber Temperature Calibration 92. Door Temperature Calibration OB... Infrared CO2 Sensor Calibration 94 Low Sample Switch Adjustment Section No. 10 101... . Preventative Maintenance “Tubing 102.. +COg Supply Filter 103. . -Air Inlet Filter 104... «Infrared COg Sensor Pump System Filters 105... IR Autoflow Chamber 106 «IR Autoflow Water-Jacket 10.7. IR Autoftow Incubator Log Section No. 11 Section No. 12 «Etror Indicators and Troubleshooting Replacement Parts List DRAWINGS BOD-02524 ... «Specification Drawing, NU-2500E BCD-02525... «..Speeification Drawing, NU-2600E BCD-02526 . «Specification Drawing, NU-2700E ACD-02873 « «Shelf Installation BCD-02059... ULLINICE System Front Control Panel BCD-02527 . {IR Auloflow Rear Panel BCD-02528 “Control Center Top Panel Removal Procedure BCD-02355 : Lower Heater Replacement BCD-02958 . «Door Heater Replacement BOD-02529 . | .NU-2500E Electrical and Tubing Connections BCD-02590 . [NU-2600E Electrical and Tubing Connections BCD-02531 . 1LUNU-2700E Electrical and Tubing Connections. BCD-02532 . 111 NU-2500E Electrical Schematic BCD-02533 . INU-2600E, 2700E Electrical SchematicIR AUTOFLOW AUTOMATIC CO2, WATER-JACKETED INCUBATOR MODELS NU-2500E, 2600E, 2700E OPERATION AND MAINTENANCE MANUAL 1.0 General Description “The NuAlre IR Autoflow Automatic COz water-jacketed incubator has been designed te provide a reliable controlled in vitro environment for optimum tissue cell culture growth. Five parameters contribute 10 optimum growth conditions. These are: Humidity Precision temperature control Precise COp control Sterlty Reliability Like all NuAire equipment, the IR. Autotlow has been designed to provide the highest quality standards of performance with matching computer technology, precise temperature control and infrared CO gas control system combining state-of-the-art technology with years of design, quality and manufacturing experience. In order to accomplish the foregoing objectives, the IR Autoflow features the following: 4.4 Extra Large (60.56 liters) Water-Jacket — Each Chamber ‘The outer stainless stee! wall is lined with a space-age insulation providing a RS. rating, minimizing heat loss. The large (60.56 liters) water-jacket utilizes water, one of natures best “sinks” of heat. Its high capacity to hold heat makes it the ideal medium to surround a chamber in order to obtain temperature uniformity. In fact, the ability of materials to hold heat, called the specific heat, uses water as the comparative standard, The large water-jacket surrounding the chamber permits the water to circulate within the jacket, producing a temperature uniformity of +0.2°C. The larger the mass, the less susceptible within the chamber to environmental fluctuations outside. It also adds cabinet stability for the growth of vibration sensitive cells. 4.2. Nufire Incubator Control Electronics (NICE) ‘The NuAire Incubator Control Electronics (NICE) is a state-of-the-art microcomputer based control system specifically designed to service the precise control requirements of the chambers environment, providing optimum programmable conditions for culture growth, The NICE microcomputer is “user friendly” with status indicators, LED display of control parameters and nine digit touch control key pad with additional select and enter keys permits efficient operator entry of data, ‘The NICE microcomputer is supported with Read Only Memory (ROM) containing executable software, Random Access Memory (RAM) for temporary storage and Electronically Erasable Programmable Read Only Memory (EEPROM) for control set points and parameters. The EEPROM provides for indefinite storage of these values during periods of power off or power interruption (power fault tolerant) ‘The NICE microcomputer includes ternperature and COp backup systems. The temperature system is monitored with triple redundant temperature sensors. Each individual sensor monitors the incubator chamber and the other sensors. If one sensor is out of calibration, the incubator will continue to operate as normal and identify a fault condition, The COz control system uses an infrared COp sensor, as described in Section 1.3, to monitor the CO concentration. If a CO2 fault condition occurs, the NICE microcomputer will acknowledge the fault, disengage the infrared CO2 ‘sensor, purge the chamber to an ambient condition, check the CO2 setpoint and purge COg into the ‘chamber reaching the setpoint level. These backup features assure the integrity of the chamber ‘environment is held for optimum cell growth, ee13 14 18 NICE includes 2 complete internat diagnostic software package that permits fault isolation detection own to the falled component. Entering "974" allows 2ccess to any diagnostic function including microcomputer output and input tests. Intrared CO Sensor System ‘The NuAlre IR Autofiow employs a Single Gas Analyzer for carbon dioxide. This innovative analyzer utilizing a filer correlation technique for nondispersive infrared analysis of COp offers unparalleled stability. This analyzer consists of an optical bench incorporating an infrared source, rotating filter wheel with two optical filters, sample cell, and infrared detector. It accurately monitors CO2, concentrations over extencied periods of time without need of calibration, ‘The analyzer, utlizing a ratio technique for signal measurement and accurate temperature control of the bench, provides unparalleled stability which will enhance the 1R Autofiow’s performance, decrease the maintenance requirements, and save you money on ancillary equipment required to support the gas analyzer measurement. The gas analyzer measurement of CO is independent of humigity and temperature variations within the chamber. All Stainless Steel Construction All NuAire Autoflow chambers (both interior and exterior) are constructed from stainless steel, which provides an easily cleanable (for decontamination) inert surface that does not in itself promote biological growth. In addition all shelves, shelf supports, guide rails and gasket guards are easily removable and can be autoclaved to remove contamination. The sample gas outlet required for the gas analyzer Is protected with HEPA fier. All interior surfaces are smooth and free of burrs that may harbor contamination, Remember: the chamber is not selective. The growth environment is applied to all microorganisms within the interior, equally. Humidity Humidification of the chamber is achieved through the process of water evaporation from a water reservoir placed within the autoflow chamber. In addition water condensation, which actually lowers the relative humidity within the chamber, is prevented by having ail interior surfaces exposed to the constant temperature water-jacket, or heated separately via an integrated control that regulates door heat to maintain a condensalion-free glass door. Without condensation and with an easily cleanable stainless steel interior, the probability of contamination is minimized, 2.0 Performance Parameters 24 22 23 24 25 26 ‘The IR Autofiow’s exterior is constructed of 16 gauge, type 304 stainless steel with the interior being 20 gauge, type 304 stainless steel, with all seams fully welded, ground and polished. All exposed ledges are deburred to insure that there are no sharp edges. The exterior is finished in a textured polyurethane finish, which is resistant to chemicals and easily cleaned using mild household detergents, Each chamber’s water-jacket holds 60.56 liters of water, that in conjunotion with the NICE control system provides an interior chamber temperature uniformity of 02°C at 37.0°C, ‘The IR Autofiow’s microcomputer temperature control system polls three temperature sensors (two in water-jacket, one in chamber) and compares the values to the setpoint and executes a time proportional control algorithm that energizes a solid-state switch, supplying power to the heater. ‘The CO2 percentage is controlled by an infrared gas analyzer which is temperature compensated and non-sensitive to humidity variations. Calibration of infrared gas analyzer is accomplished simply through a front panel diagnostic procedure to assure accuracy and minimize downtime, Automatic recovery of CO2 after a door opening to 5% CO2 within 2 minutes for a single chamber with minimal overshoot, ange27 28 29 2.10 an 212 243, 214 245 218 2a7 ‘The outer door includes @ radiant heater in order to minimize condensation on the inner glass door, Inner glass door temperature is monitored by a separate temperature sensor and adjusts from the front panel key pad, A magnetic outer door gasket helps to insure a tight seal against the cabinet. “The inner glass door is 5mm tempered with smooth-ground edges and seals airtight against a U- grooved silicone rubber gasket, The door latch Is cam action. An all solid-state magnetic switch monitors door motion. All electronic controls are modular and easily removed through the front or top service control ‘center, All control electronics are protected with an 5 or 10 amp circuit breaker that may trip at 110% of load ‘ating but will {rip at 145% of load rating in less than 2 seconds. Should the circuit breaker open (red pop-out button will appear), merely depress to reset ‘A water fill access port is provided an the front of the IFi Autoflow. A % inch NPT plated brass fill plug is removed and a 4 inch NPT to 9.5mm hose adapter is provided. ‘The IR Autotlow has heavy-duty adjustable leveling legs to compensate for uneven laboratory surfaces. ‘The entire interior shelving assembly I easily removable for decontamination. The shelves are constructed from 18 gauge, type 304 stainless steel and are square (489mm x 489mm). ae {A through-wall access port is provided for operating electrical appliances such as roller apparatus, rockers, etc ‘A.CQg sample port is provided on the front panel to check the concentration of GOg in the chamber. ‘A.water level float switch is provided to monitor the level of weter within the jacket. ‘A water-jackel drain Is provided on the bottom of the unit for ease of maintenance, 3.0 Models and Features NuAire offers three configurations of the basic Model NU-2500E Water-Jacketed IR Autoflow Automatic. ‘These are (1) Model NU-2500E the basic single chamber unit, (2) Mode! NU-2600E a side-by-side unit and (8) Model NU-2700E two single chamber units vertically stacked over/under. 3a 32 Dimensions (See also specification drawings BCD-02524, BCD-02525, and BCD-02526,) Model NU-2500E Model NU-2600E + Mode! NU-2700E Overall Dimensions: Single Unit Side-by-Side Unit Vertical Unit Width: 635mm 1308,10mm 635mm Height: 1009.65mm. *1909,65mm 11960.55mm. Depth: 685.8mm 685.8mm 685.8mm ‘The side-by-side IR Autoflow Is two independent chambers with individual controls. Doors open from the center. Standard Features 100% stainless steel chamber construction Large capacity water-jacket (60.56 liters) NuAire Incubator Control Electronics Temperature Range: 5°C above ambient to +55°C Chamber temperature uniformity: +0.2°C at 37°C. © Temperature sensitivity: £0.0125°C. © COp Range: 0-20% © CO Accuracy: +0.1% © CO? Recovery to 5%: 2 minutes33 34 ‘Standard Items Packed With Unit (4) Stainless Stee! Shelves (8) Stainless Stee! Shelt Brackets (4) Stainless Stee! Shelf Bracket Supports (1) Fill Por Fitting ®.5mm Tube x ¥" NPT (1) Gas Tube with Fiter (1) 2 meters, 95mm Tube « (4) Heavy Duty Leg Levelers © (1) IR Sensor © (1) Electrical Cord 2.4 meters Accessories ‘Model NU-1550 Automatic CO Tank Switch Model NU-1552 COz Alarm Model NU-1583 Stacker Tray (for vertical unit stacking) Model NU-1555 Full-size Water Pan with Supports, Model NU-1886 Additional Tubing Kit Model NU-1587 Additional Stainless Stee! Shelf with Supports Model NU-1589 COp Analyzer Fyrite Kit Model NU-1581 Replacement Fluid for CO2 Analyzer Fyrite Kit 0-20% (2 bottles/carton) Model NU-1585 Platform w/casters, Mode! NU-1586 Platform Mode! NU-2562 Chart Recorder Interface (Single Chamber) - Mode! NU-2563 Chart Recorder Interface (Dual Chamber) Mode! NU-2581 Communications Interface (RS-232/RS-485) ‘Mode! NU-2587 Comm/Chart Rec. Interface N ‘Mode! NU-2564 Security Key Lock ‘Mode! NU-2560 (Internal) Automatic CO Tank Switch Mode! NU-2565 Humidity Display (Single Chamber) ‘Mode! NU-2568 Humidity Display (Dual Chamber) eo ecevcescccccccce 4.0 Test Performance and Procedures [All equipment is thoroughly inspected at the NuAire Factory at the time of shipment. Quality control is maintained by constant surveillance over the product, beginning at the receipt of purchased material and concluding with a final inspection which certifies the IR Autofiow’s performance to the specifications. In all instances where product quality cannot be easily assessed on the end item (i. water-jackat leak tightness), the product is inspected during sub-assembly fabrication. The following test procedures are conducted on each cabinet and a copy of the test report is included with each unit. 4a 42 Visual Inspection 4.11 Each IR Autoflow is visually inspected to insure that the interior is clean and free from scratches, nicks and burrs and that all welds, both interior and exterior are ground and polished smooth, 4.1.2 Painted surfaces are inspected to be free of scratches, nicks, insufficient covering and runs. 4.1.3 The door opens/cioses freely without binding of the hinges. The vinyl gasket seals the inner lass door tightly. The glass door is free of scratches. Electrical Tests 42.1 Electrical Leakage Test AG. electrical leakage shall not exceed 500 microamperes using a Simpson Model 229 or equivalent testor.4.22 Dielectric Voltage —Withstand +800 volts is applied between dead metal parts and the hov/neutral power source lead with no electrical breakdown using a Slaughter Model 101-225, 43 Functional Tests “The following functional tests are performed on every unit at the end of a continuous 48 hour burn in period. 43.1 NICE Control Systems All diagnostic functions are exercised to insure proper operation of NICE Control System, components and alarms. 432 C02 Control Each unit is calibrated to function at 5%. COp is introduced into the chamber and allowed to stabilize for ten minutes at 5% concentration. The concentration is checked with a fyrite. Each unit is monitored during the 48 hour burn in period and only accepted with zero failures. 433 CO2 Recovery Each unit is exercised for CQ recovery time at the end of the 48 hour burn in period. The door is ‘opened for 1 minute to deplete the CO2. After the door Is closed the unit shall recaver to 0.2% within 2 minutes. A dual chamber unit will recover each chamber to 0.2% within 4 minutes, 5.0 Warranty NuAire, Inc. warrants that it will repair FOLB, its factory or furnish without charge F.O.B. its factory a similar part to replace any material in its equipment within one year after the date of sale if proved to the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall be returned, properly identified to the company at its factory, charges prepaid. Factory installed equipment or accessories are warranted only to the extent guaranteed by the originat manufacturer and this warranty shall not apply to any portion of the equipment modiied by the user. Claims under this warranty should be directed to Nui, Inc. setting forth in detail the nature of the defect, the date of the initia! installation and the serial and model number of the equipment. NuAire, Ine. warrants the water-jacket and will repair or replace FO.B. its factory or furnish without charge FOB. its factory the water-jacket within five years after the date of sale if proved to the satisfaction of the ‘company to have been defective at the time it was sold. This warranty shall not apply to any NuAlre product or part thereof which has been subject to misuse, abuse, filling the water-jacket improperly, using additives in water-jacket, accident, shipping damage, improper installation or service, or damage by fire, flood or acts of God. If the serial number of this product is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety. ‘The warranty is for the sole benefit of the original purchaser and is not assignable or transterable. 6.0 Shipments NuAire, Inc. takes every reasonable precaution to assure that your Incubator arrives without damage. Motor carriers are carefully selected and shipping cartons have been specifically designed to insure your purchase. However, damage can occur in any shipment and the following outlines are steps you should take (on receipt of a NuAlre Incubator to be sure that if Gamage has occurred, the proper claims and actions are taken immediately. 6.1 Damaged Shipments 6.1.1 Terms are F..B. factory, unless stated otherwise. Therefore, itis important to check each shipment before acceptance.6:12 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy of the freight bill, Then an inspection must be made to verify the claim against the cartier. This inspection is the basis of your filing the claim against the carrier 6.1.3 If concealed damage is found, itis absolutely necessary to NOTIFY THE FREIGHT AGENT AT ‘ONCE, and request an inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain date and that he failed to comply with the request. This along with other papers in the customer's possession will support the claim. 7.0 Installation ‘The IR Autoflow is fastened to a base skid and itis usually the best procedure to leave the skid attached until the IR Autofiow is located in its approximate position, to facilitate ease in handling. The base skid can then be removed by removing the four bolts holding the cabinet to the skid. Examine the IR Autoflow carefully. INSPECT both the exterior and the interior of the IR Autoflow for any transit damage before discarding the shipping crate (see Section 6.1.3) 7A 72 3/8-16 » 2” HEX HEAD BOLT (4) Location In locating the IR Autofiow, consider all possible conditions that might atfect its performance as well as laboratory procedures for is intended purpose. Do not locate near heating or cooling ducts, or next to equipment that generates heat (steam radiators, stoves, ovens, autoclaves, etc.). Avoid direct sun rays and rapidly moving air currents. The IR Auloflow needs even heat loss on all surfaces in order to maintain an internal temperature variation of less than 0.2 degrees C. As a result, a minimum of 50mm must be allowed between the rear and sides of the IR Autoflow and any walls, partitions or ‘obstructions to facilitate adequate convection of air around the IR Autofiow’s water-jacket. For maintenance/service purpeses the control center top containing the electronics should remain accessible. Four (4) screws (two on each side) hold the control center top (see drawing BCD-02528). Leveling “The IR Autotlow should be leveled prior to filing with weter, and should rest firmly on the bench or floor. Uneven water levels may cause false “add water" indications on the front panel as well as to afiect the water circulation paths within the water-jacket, which could cause condensation on the ‘walls of the chamber. Leveling feet are provided for this purpose and installed into the base of the IR. Autofiow, using the same holes the base skid was fastened to. Turning the adjustable leveling feet counterclockwise raises the IR Autoflow. The leveling feet height should be approximately 6mm. below the IF Autoflow base.a ee eee ee ee73 7A 75 78 ‘Sholf Installation Before installation of the shelves, NuAlre recommends to decontaminate all surfaces within the Interior chamber, glass door and outer door with gasket. They can be wiped down with a disinfectant of 70 percent alcohol, 2 percent alkaline glutaridehyde or similar non-corrosive antimicrobial agent. Provided with each IR Autoflow are four shelves. The shelves are easily installed per Drawing ACD- 02873, by attaching the guide supports to the stainless steel pins in the interior of the chamber. ‘Additional shelves and shelf guides are available. Electrical ‘The electrical supply circuit to the IR Autotiow must conform to all national and local electrical codes. Consult the 1R Autoflow's serial-data plate, located inside the door, for voltage, eycle, phase and ampere requirements before making connection, Plug the 2.4 meter service cord securely into GROUNDED OUTLET. VOLTAGE SHOULD NOT VARY MORE THAN 5% FROM SERIAL PLATE RATINGS. A separate branch clrouit is recommended to prevent possible loss of product due to overloading or failure of other equipment on the same circuit Precaution for IR Autoflow Filling ‘To prepare the IR Autoflow for filling, turn on Main Power Switch located on Back Panel and Set Mode Switch to Standby/setup. The digital indicator should fight up 2s well as the add water light. Filling and Draining the Water-Jacket ‘The fil port plug is located on the front top left side behind the exterior door. Remove the plug and Install the 4" NPT x 9.5mm tube adapter. Place the 9.5mm tubing over the adapter and connect the other end to either a funnel or serrated tap. USE SINGLE DISTILLED OR DEMINERALIZED WATER to fill the water-jacket. No purer than 10 megohm water should be used. While the unit is on, fil the water-jacket until the “ADD WATER’ lights turns off. During the water-jacket fill operation, slightly jar the unit to remove any air pockets from forming in the water-jacket, Add an additional iter of water, remove the tube adapter and replace with the fill port plug, CAUTION Be sure to position and level the IR Autoflow as desired before filing water. DO NOT OVERFILL! ‘The water-jacket requires no anti-bacterial agents. The IR Autoflow already incorporates a copper tube producing copper sulfate which eliminates bacterial growth within the water-jacket. ABSOLUTELY NO CHLORINATED OR HALOGEN MATERIALS ARE TO BE USED IN THE WATER- JACKET,a ‘A safaty overfill port is located next to the fill port plug $0 if overfll does occur, the water will be relieved through the port. ‘The water-jacket can be drained from the drain plug located on the bottom left side. The drain uses & ball valve to control the drain water. I the valve stem is horizontal, the drain is closed and if itis vertical, the drain is open. A white safety plug must be removed prior to draining located on the bottom of the ball valve. It also insures no leakage will OCCU. OF Ul pant C02 Tube Connection Included with every IR Autoflow is a tubing kit consisting ot (1) 2 meter vinyl tube and (1) 0 mm polypropylene 0.3 micron HEPA filter. 7.74 CO2 Supply 1. Before the COg supply is turned on to the IR Autoflow, fll the water-jacket and set the temperature. (See Section 8.) CAUTION CO. pressure to the IR Autofiow inlet is rated at 1.4 BAR or 20 PSIG. DO NOT exceed 2.0 BAR or 80 PSIG, 2, COg of medical grade is recommended. 3. A two-stage pressure regulator, Linde #998943, or equal, is recommended. 4, DO NOT USE a single stage regulator. 7.7.2 CO2 Regulators ‘The regulators high pressure stage direct from supply cylinder must have range of from 0 to 140, BAR or 0 to 2000 PSI, This gauge indicates actual tank pressure. The low pressure stage should have ‘a range of irom 0 to 2 BAR or 0 to 30 PSI (6 BARS maximum). This gauge will indicate the actual (CO2 pressure into the IR Autoflow system. Some single stage COg pressure regulators have two gauges. USE A TWO STAGE REGULATOR. All NuAlre Autoflow’s use CO2 in such small quantities that precise metering of COp input pressure is important for maximum performance of the IR Autotiow. To connect the regulator: First open the CO2 cylinder slightly, for an instant, (This is termed “cracking the valve”) This will blow out dust or dirt that may have collected in the valve outlet. BE ‘SURE to keep your face away from the valve outlet to protect your eyes from dust or dirt. Second, MAKE SURE the regulator pressure-adjusting screw is released by turning it counterclockwise until it turns freely. Third, attach the regulator tothe cylinder valve and tighten the connection nut with @ wrench. BE SURE DISC GASKET IS IN PLACE BEFORE MAKING CONNECTION. 7.73 COp Connection Connect the CO supply from the low-stage of the two-stage regulator, to the Smm inlet fiting located on the incubator backpanel, The filter should be inserted downstream of the fow-stage regulator |before the 3mm inlet nozzle to the IR Autoflow. Observe proper flow orientation of the filter (look for “in” on filter). The tubing is easily cut with a sharp knife, 7.74 CO2 Supply Adjustment With the regulator OFF (ie, fully counterclockwise), open the cylinder valve slowly; usually 1 t0 2 turns is sufficient, NEVER STAND IN FRONT OR BEHIND THE REGULATOR WHEN OPENING THE VALVE. ALWAYS ‘STAND TO ONE SIDE. ‘The cylinder tank pressure should read 48 to 55 BAR or 700 to 800 PSI, more or less. depending on the temperature of the cylinder. Next turn the regulator’ pressure adjusting screw clockwise until the low-pressure gauge reads 1.4 BAR or 20 PSI, The COg connection is now complete. NOTE OSHA requires the COg tanks to be physically restrained (ie. via chained to wall) to prevent accidental damage to cylinder. If Model NU-2560, an Automatic Tank Switch is purchased; separate installation instructions are provided, 8.0 IR Autoflow Operation ‘The IR Autoflow is designed to provide a sterile, constant temperature and high humidity controlled almosphere for optimum growth of tissue cell cultures or other organisms requiring this precise environment. To operate the IR Autoflow properly, the following parameters must be reviewed, carefully set and/or prepared. Bat 82 Sterility ‘The environment provided by the IR Autofiow is not selective. As a result any contamination within the chamber is subjected to the same environment as the specimens. Therefore, before placing any cultures in the IR Autoflow, the shelves, and shelf brackets should be sterilized. The interior side walls, top, bottom, door as well as the gasket should be wiped clean with a 70% solution of isopropyl alcohol or other disinfectant, to remove any contamination. Use mild detergent to clean exterior of IF, Autoflow, Humi Humidification of the IR Autoflow is achieved through the process of water evaporation (vapor water pressure) from a stainiess steel water pan (NuAire Model NU-1855) placed on the bottom of the IR Autoflow shelf rack, Materials of diferent thermal resistance (.e. glass, plastic) do not offer sufficient thermal recovery and are not recommended for use. Although some metals offer better thermal coofficients than stainless steel, dissimilar metals cause electrolysis in the acid atmosphere (carbonic acid) and should never be used, or placed within the IR Autoflow's chamber. Use only distilled or mineral-free water in the stainless steel pan. The water should be changed at least once a week, preferably more often, Flooding the bottom of the IR Autofiow 1S NOT RECOMMENDED since itis dificult to change the water weekly and almost necessitates the use of chemicals which are not recommended and may damage the stainless steel. Also it promotes condensation on the IR Autoflow’s inner walls because it steals the natural convection, heat flow: through the inner chamber and condensation points occur. Humiaity recovery will be back to 95% within 15-20 minutes atter a 30 second door opening with @ water reservoir area of 361 square inches (e. a NU-1555 full-sized pan). Contamination in the water pan may be avoided by adding a small amount of copper sulfate to the water pan after every decontamination of the chamber. Condensation on the door walls, top or bottom of the chamber indicates an incorrect balance of door heat which should be calibrated to allow the NICE main temperature control to heat the chamber, not the door heater,as NICE System Introduction ‘The NuAire incubator Controt Electronics (NICE) system is designed to service the control requirements of the IR Autofiow incubator chamber, Temperature and COg level are controlled by preset values to provide optimum conditions for culture growth within a chamber. NICE controls by sampling input sensors, setting control outputs, and displaying status on a control panel. Operator input is coordinated through the control panel keypad and status displays. Figure 1 shows the various inputs and outputs of the system. ‘The NICE is a stale-ol-the-art microcomputer based system that provides: 8.3.1 Single or dual chamber contro! in a single electronic package. 83.2 Enhanced information presentation. The control panel presents: © Chamber temperature (set point end actual) C09 level (set point and actual) Optional, humidity level (actual) Optional, security key lock Time of day Culture days Output and alarm status: © Water-jacketed heater status Door heater status C02 control status ‘Water-jacket low water status Chamber door ajar System alarm condition status COp tank selection oo, LE, ow AC POWER Figure 1. 1R AUTOFLOW CONTROL INPUTS AND OUTPUTSaa 83.3 Simplified operator controls. The control panel is operated using a numeric keypad with the addition of SELECT and ENTER keys. As the SELECT key is repeatedly depressed, the display ‘shows the value corresponding to the indicated parameter. Each depression advances to the next parameter. Entry of a numeric value (with mode switch in standby/setup) followed by depression of ENTER changes the control set point. 8.3.4 Automatic notification of abnormal situations. The red LED on the control pane! indicators will light to indicate a fault within the system. Such faults include: © Power Failure © NICE System Intermittent © Control Fault (2. temperature exceeds set point tolerance) © Sensor Out of Calibration 8.3.5 Provision for add on expansion capability. Options include: © Remote communication capability (RS-202/RS-485) ‘Local printer support for hardcopy of monitored data Chart recorder output of monitored values Automatic tank switeh Humidity display 83.6 Diagnostic and calibration assists. By entering a special code when resetting the culture days value, diagnostic mode is entered. In this mode: © Individual analog inputs may be displayed to assist calibration Individual outputs may be forced to an on or off condition © Individual digital inputs may be displayed © Front pane! lamps may be tested ‘© Memory and internal processor diagnostics may be selected © All options may be individually tested © System configuration parameters may be altered NICE packaging is designed such that all modules are easily removed and replaced, All external wiring is connected through sorew terminal connectors. All expansion modules are plug in. All printed circuit boards are held in place with machine screws and all connector blocks to expansion modules are keyed to insure correct placement. NICE System Front Control Panel ‘The NICE system front control panel contains the following functions described in detail (see Drawing BCD-02359). 8.4.1 Jacket Heat Status LED ‘The jacket heat green LED indicates when the chamber heater is turned on. 8.4.2 Door Heat Status LED ‘The door heat green LED indicates when the door heater is turned on. 8.4.3 Door Ajar Status LED ‘The door ajar red LED indicates when the inner glass door is not closed. The LED acts upon a magnetic switch located along the lower right corner of the inner glass door. 8.4.4 Low Water Status LED ‘The low water red LED indicates when the water-jacket requires additional water. If the low water light is lt, the water-jacket should be filed as soon as possible to avoid uneven heating of the chamber. 8.4.5 Control COp Status LED ‘The control CO green LED indicates when the COp control valve is open and flowing CO2 into the chamber.85 8.4.6 Low Sample Status LED ‘The low sample red LED indicates when the infrared sensor pump system has insulficient flow. This would be caused by the 0.3 micron recirculation filter being filled with contamination or condensation. 847 CO2 Tank 1 Status LED (Option) ‘The C02 tank 1 green LED indicates when the IR Autoflow is consuming COp from tank 1 8.4.8 CO2 Tank 2 Status LED (Option) “The COg tank 2 green LED indicates when the IR Autotlow is consuming COg from tank 2. 8.49 Alarm/Error Status LED ‘The alarm/error red LED indicates a failure condition or an invalid entry of data. A blinking LED indicates a keyboard entry ertor, press "SEL" key to clear. If the LED is on continuously a catastrophic condition exists. A catastrophic temperature control condition will de-energize the safety relay and cause the chamber to cool below the set point. Then again the temperature control will try to control. CO and humidity will not catastrophically fail, bul cause the alarm to blink and continue to try to control. 8.4.10 Sample Port ‘The sample port is provided to measure CO2 percentage manually with a fyrite COg indicator, or other suitable instrument. 84.11 Value Indicators ‘The value indicators, located above the mode switch, indicate the value being shown in the four digit display. The LED corresponding to the value being displayed willbe lit. The red LED (left side) indicate setup mode selection, The green LED (right side) indicate run mode selection, Normally, only a single red or green LED willbe lit, If both LEDs are lit, an abnormal situation has ‘occurred. A power failure will cause both Time of Day LEDs to be lit. If the system is in run mode, the red LEDS in the temperature, CO percent, or RH percent (optionally) indicate a control or sensor fault, 8.4.12 Mode Switch ‘The mode switch is used to select the operating mode of the IR Autoflow chamber, standby/setup or run, 8.4.13 Selection and Entry Keypad ‘The selection and entry Keypad (KEYPAD INPUT SHOULD BE DONE WITH FINGER ONLY, DO NOT USE PENCIL OR SHARP INSTRUMENTS), located at the right of the control panel, is used for all operator interaction with the NICE system. The "SEL" key is always active, repeated depression of this key causes display of the next value in sequence as listed for the value indicators in a round robin fashion, When the NICE system is in setup mode, the “ENT” key becomes active and allows the operator to alter set point values. In run mode, depression of the "ENT" key will Clear the failure indicator corresponding to the value being displayed. IR Autofiow Rear Pane! ‘The IR Autoflow rear control panel contains the following functions described in detail (see Drawing BCD-02527). 85.1 Power Cord The power cord is 2.4 meters in length, type “SVT" molded plug, allowing for long life and easy cleanabitity. 85.2 Giroult Breaker AAl'control electronics are protected with an 5 or 10 amp circuit breaker that may trip at 110% of toad rating but will trip at 145% of load rating in ess than 2 seconds, Should the circuit breaker open (red pop-out bution will appear), merely depress to reset.86 8.5.3 CO2 Inlet ‘The COz inlets provide a fitting for standard 3mm O.D. clear vinyl tubing. Be sure to follow the recommended inlet pressure to insure proper flow rates and consistent COg percentage readings. 85.4 Air Inlet ‘The air inlet provides a fitting for standard 3mm O.D. clear vinyl tubing. The air inlat is provided with 150mm of tubing with 0.3 micron filter. This is a free air supply, DO NOT CONNECT with pressurized source. 8.5.5 Power Switch ‘The power switch, located at the right of the rear panel, controls all power to the NICE system. When the power switch is on, the NICE system is energized. 8.5.6 Intemal Tank Switch (Option) The internal tank switch is an option which is customer installed in the field or factory installed at the time of manufacture, The tank switch performs the critical back-up function of switching tank 1 and ‘tank 2 and back again when each depieted tank is replaced, 8.5.7 Communications (Option) The communications is an option which is customer instaled in the field or factory installed at the time of manufecture. The communication option provides fortwo way communication withthe control system utilizing elther the RS-232C or RS-488 interconnect standards. AS-232 provides a short range (16 meters) simple point-to-point connection, RS-~485 provides for multiple connections up to 32 NICE controllers on a single two or four wire twisted pair with a RS-485/RS-422 device NICE utilizes a ten bit character frame with a start bit, eight data bits (with no parity), and stop bit for all communication. The baud rate is selectable, To permit multiple NICE systems to share a common ‘communication cable, all communication is half duplex. NICE does not echo characters received on the communication line and does not respond until a complete and valid command is received. 8.5.8 Chart Recorder (Option) ‘The chart recorder is an option which is customer installed in the field or factory installed at the time of manufacture. The chart recorder output provides an analog signal corresponding to a selected chamber parameter. One signal is provided per chamber supported. The signal is jumper selectable for either 0-2.58V or 0-10.24V, Jumpers J1 and J2 select the analog range for chambers one and two respectively, Selection of the chamber parameter to be charted is done through the NICE front panel Temperature, COz percent or RH percent may be selected for each chamber, but only one parameter can be charted at one time, 8.5.9 Humidity Display (Option) ‘The humidity display is an option which is customer installed in the field or factory installed at the ‘time of manufacture. The humidity monitor uses a solid state analog humicity sensor and indicates the AH percentage value on the display. The humigity is produced from the stainless stee! water pan {NuAire Model NU-1565). NICE Run Mode Operator Interactions {in general, there is no need for operator interaction in “run'* mode. Operator interaction is provided to permit inspection of current operating conditions. To view a specific parameter, simply press the "SEL" key until the green LED appears next to the desired parameter. Repeated depression of the "SEL" key advances in a round robin fashion to the next value parameter. {fan abnormal condition has, or does, exist for a particular parameter, the red LED next to the parameter will be lit. This is not a catastrophic alarm condition, which could harm the tissue culture celis. However, it does indicate an operational abnormality and should be checked. To acknowledge the abnormality, simply turn the mode switch to standby/setup and back to run to clear the NICE system. The red LED will then extinguish. Hf the abnormality still exists, the red LED will again be It. Do illustrate the abnormality, the chamber temp red LED is lt. Turn the mode switch to standby/setup ‘then back to run. Let run normally, if red LED doesn't come back on, everything is normal. If the red LED does come back on, enter into diagnostic mode and recalibrate tersperature offset (see Section 9.1). Ifthe red LED still remains lit, call for service,ar 38 “The following list provides reasons for abnormality. Also, use the troubleshooting guide (see Section 11.0), Parameter Reason for Abnormality Time of Day Power Interruption Chamber Temp NICE System Intermittent, sensor out of calibration or sensor fault C02 Level NICE System Intermittent, sensor out of calibration or sensor fault RH Percent NICE System Intermittent, sensor out of calibration or sensor fault Catastrophic faults would cause the NICE system to alarm/ertor and limit control of the chamber ‘environment, For example, if the chamber temp reached 1°G above setpoint, the alarm/error red LED ‘would light, the NICE system would interrupt power to the heater until the chamber temp returned to setpoint. The heater would then again try to control. If the chamber temp again reached 1°C above setpoint, the alarm/error would again interrupt power to the heater. This cycle would continue until ‘the unit is checked and the fault corrected, NICE Setup Mode Operator Interactions 8.7.1 Chamber Temperature Setpoint, COp Percent Setpoint, Humidity Percent Setpoint Setpoint values are entered by selecting the desired parameter and pressing the “SEL” key, each depression will advance, in a round robin fashion, to the next value parameter. The value of the selected parameter will be shown in the display in the form “XX.X". To enter a setpoint, pertorm the following: © Turn mode switch to stendby/setup. © Press "SEL" key to desired parameter. © Press desired entry, then the “ENT” key. (NOTE: decimal point remains constant.) © Turn mode switch to run, Ita mistake is made, simply reenter the parameter as before, ‘As the first key is entered, the value display will clear and the digit wll be placed in the rightmost position. Each subsequent digit will cause the display to shift left one digit to permit the new digit in the rightmost position, Any number of digits may be entered, but only the last four will be valid. IF A MISTAKE IS MADE, simply enter zeros until the value is cleared and start again, f the “ENT” key is pressed with an invalid value, the “Alarm/Error” LED wil blink and the number must be reentered. 8.7.2 Time of Day To enter the time of day use the same procedure as above, press the “SEL” key until the parameter LED for time of day is lit. The current time will be displayed in the value display in the form of “HH.MM'’, To alter the time, enter the hour and the minute in 24 hour form (00.00 is midnight). The first digit pressed will cause the display to clear and subsequent digits will be entered from the right. Press the "ENT" key to complete the entry and start the clock, Seconds will start accumulating from zero when a valid time is entered, Invalid entries will cause the “Alarmn/Error” LED to blink and a new time must be reenteres. 8.7.3 Culture Days Culture days represent a count of the number of days that the chamber has run since resetting the culture day count. The count is advanced on each roll over of time of day clock. To enter culture ays use the same procedure as above, simply enter a new count and press the “ENT” key. NICE Diagnostic Interactions ‘Ths NICE system provides for complete diagnostic procedures to be performed in the field. Selection of the diagnostic function is entered by performing the following: © Turn mode switch to standby/setup, © Press "SEL" key to culture days. © Press "9794" "ENT” key, display blinks "FUNC 0” ‘The diagnostic allows the operator to perform offsets and check individual components for proper operation. Below, is a list of all the diagnostic functions with their associated number. After. ~ performing a diagnostic function, press “SEL” key to zero and clear the current command ang prepare for the next.While in diagnostics, the display will alternate between showing the selected diagnostic value and the diagnostic number. NICE Diagnostic Functions x Internal Tests 2x Show/Adjust Analog Inputs - Exit Diagnostics 20 Jacket temperature 1 - Lamp/LED test 21 - Door temperature 2 CO Sensor Calibration 22 - Chamber temperature 3-Memory Test 23 - Safety temperature 4~ D/A Chart Output test 24 - COz percent (or Busy/OFF) 5 - Communication Loopback test 25 ~RH percent @- Reload Default Configuration 9- Enter Configuration Mode 8x Show/Force Digital Outputs 30 - Safety relay (Enab/Safe) ‘1x Show Digital Inputs 81 - Jacket heater 10 - COp Tank pressure (lank switch option) 82 - Door heater 11 Door status 83 - Air control valve 12- Low water status 34 - CO contrat valve 13 Low sample status Examples: 1) Press "11" "ENT" key, the door status should be closed. To check, open the glass door and display should then indicate open. Press “SEL” key to zero command, 2) Press “1" “ENT” key, the display and indicator lights should activate through a sequence to identity any display faults. Press “SEL” key to zero command, 9) Press “a4” “ENT” key, the CO control valve should display and indicate off. To exercise any digital ‘output, press any "1-9" key to turn on the valve and the “O” key to turn off the valve. Note: The display and status indicator LED should also indicate an on or off condition. Press "SEL" key to zero command, xFunctions — Internal iagnostics Function 0 — Exit Diagnostic Function Mode Selection of this function returns to setup of Culture Days. Function 1 — Lamp Test ‘This function will turn all individual LED's and value segments on, sequentially turn them all off and repeat the sequence until another function is selected. Function 2 — COg Sensor Calibration This function will allow the zero and span calibration of the COg sensor. A user friendly prompt will advance through the procedure to assure proper sensor calibration. Function 3 — Memory Test This memory test function will perform a non-destructive test of all random access memory in the system. Upon compietion of the test, the display will show "Pass” or “Fail. If the result is “Fall,” service is required on the unit. Function 4 — D/A Chart Output Test (Chart Recorder Option) “This function will output a stair step pattern on the chart recorder output for this chamber. The pattern will 90 from zer0 to maximum voltage then from maximum back to zero. The display will show *Chrt" while testing isin progress. Function 5 — Communication Loopback Test (Communications Option) ‘The communication testis designed to output the NICE software revision level on the communication port and to receive it back, To do this, a special test plug is needed for the communication port. If the test is successful, "PASS" is Gisplayed. If not, “FAIL” is shown in the value display.Function 8 — Reload Default Configuration a ‘This function causes a reload of the default configuration and a restart of the system. It is provides in the event that memory is destroyed, See Section 6.8.1 ‘1x Functions — Show Digital Inputs . For al digital input functions, press the “ENT” key will advance to the next input vale Function 10 ~ CO Tank Pressure (Tank Switch Option) ‘This function shows the current state of the CO2 tank pressure sensor. Th ‘r "Lo” corresponding to the pressure. 1e value display will show NOTE “This function will require that the COp inject valve (Function 34) be momentarily activated to bieed any excess pressure from the line, Function 11 — Door Status This function will display the status of the chamber door as “OPEN” or "CLOS' Function 12 — Low Water Status This function shows the current state of the water level sensor in the jacket. The value display wil show “Hi” or "Lo" corresponding to the water ievel Function 13 — Low Sample Status This function will display the flow level of air into the COg sensor. The value display will she ‘or "Lo" corresponding to the air flow level. A low flow level indicates a plugged filter. ow “Hit 2x Functions — Show Analog Input Function 20 — Jacket Temperature Function 23 — Safety Temperature Function 21 — Door Temperature Function 24 — CO2 Percent Function 22 ~ Chamber Temperature Function 25 — RH Percent For all analog input functions, pressing the “ENT” key will advance to the next value Input Function 20 — Jacket Temperature et This function shows the current value of the temperature sensor located in the water Jack Function 21 — Door Temperature This function shows the current value ofthe temperature sensor located against the inner glass door. Function 22 — Chamber Temperature ee ‘This function shows the current value of the temperature sensor lacated in the charm! Function 23 — Safety Temperature ey This function shows the current value of the temperature sensor located in the water I Function 24 ~ CO Percent This function shows the current value of CO2 percentage in the chamber. Function 25 — RH Percent (Option) This function shows the current value of RH percentage In the chamber. 3x Functions — Force Digital Outputs Function 30 — Safety Relay (Enablesate) Function 33 — Air Control Valve Funetion 31 — Jacket Heater Function 34 — COz Control Valve Function 32 — Door Heater For al Digital output functions, pressing the “ENT” key will advance to the next value outputao “These functions display the current state of the selected digital output as “OFF” or “ON.” Two possible entries may be made 10 foree a specific condition: Press 1-9 Force Output On © Press Force output Oi CAUTION (Output must be off before selecting new function. 8.8.1 Reload Default Configuration, ‘The Reload Default Configuration diagnostic function number 8 is the last effort to correct ‘operational faults which otherwise cannot be solved. By reloading the default configuration, the entire memory will be reset and ALL CALIBRATION OFFSETS WILL BE LOST. Chamber temperature, door temperature and COg sensor calibration will need to be performed following a Function 8 command. To reload default configuration, pertorm the following: (If dual unit, perform procedure on left front panel.) ‘Turn mode switch to standby setup. Press "SEL" key to culture days. Press "9734" “ENT” key, display blinks "FUNC 0”. Press "8" "ENT” key, display reads 00.0 and both time of day LEDs light, “Turn unit off and back on again via power switch on back panel ‘The procedure Is now complete, Proceed to calibrate chamber temperature, door temperature and (Cg sensor. Ii unit is a dual chamber, the right side controt panel will need to be turnes on, Perform the following on the left control panel © Turn mode switch to standby/setup. © Press "SEL" key to culture days. © Press "9734" “ENT” key, display blinks “FUNC 0" Press "9" “ENT” key, display blinks "CONF 0”, Press "10" “ENT” key, display blinks "Ch-1". (Turn right side front panel mode switch to standby/setup.) Press "1" key, display blinks "Ch-2". (Right side control panel should light up.) Press "SEL" key until display blinks "FUNC 0”. (Proceed to calibrate chamber temperature, door temperature and COz sensor for both chambers.) NICE Configuration Interactions ‘The NICE system provides tor complete configuration procedures to be performed in the field. Selection of the configuration functions should only be performed when field installing an option or ‘when calibrating the CO and humidity sensor. Since the configuration functions are the total operating parameters of the IR Autotlow, EXTREME CAUTION should be exercised not to accidentally change any operating parameters. Only access the parameters that are required, Selection of the configuration function is entered by performing the following: © Turn Mode Switch to standby/setup. © Press "SEL" key to culture days. © Press "9734" "ENT key, display blinks "FUNC 0”. © Press “S" "ENT" key, display blinks "CONF 0" Below is alist of ell the configuration functions with their associated number. After performing @ configuration, press "SEL" to zero and clear the current command and prepare for the next. While in configuration, the display will alternate between showing the selected configuration value and the configuration function number.All system parameters are as specified in Analog Significance Units (ASU). The current ASU value corresponds to the following actual parameter valves. Tomperature 1°C = 20 ASUs (COp Percent 1% = 20 ASUs RH Percent 1% = 20 ASUs. For example, Function 35, maximum above setpoint allowed is set 1°C above setpoint or 20 analog significance units. NICE Configuration Functions x Chamber Configuration 0- Exit to Diagnostic Mode 1-12 07 24 hour clock display 2- G02 enable (on/off 3- Humidity display enable (on/ott) 4- Humidity alarm enable (on/off) ‘4x System Configuration 10-1 or 2 chamber support 11-1 of 2 COg tank support 12 Default tank 18 - Communication enable (on/off} 14 - RS-282 or RS-485 Cornmunications 2x System Parameters 20- Automatic Select (seconds/5) 21 - Sonic alarm timer (seconds/128) 22- Comm baud rate (/100) 23 Comm address for protocol (0) 24 - Print frequency (minutes/0) 25 Lines per printer page (60) 28 Chart 1 D/A parameter (0) 27 - Chart 2 D/A parameter (0) 28 - Air inject time (seconds/3) 28 COg time out (minutes/10) ‘3x Temperature Control Parameters 30 - (Temperature Control 31 - Algorithm, Do Not 32 - Access) 33- 24~ 4x Humidity Control Parameters 40 - (Humidity Control 41 - Algorithm, Do Not 42 - Access) 43- nae 45 - Max above setpoint alowed (ASL/180) 48 - RH Timeout (minutes/160) 5x COp Control Parameters 50 - Air inject time before zero (seconds/120) 51 - Stabilization time after zero (seconds/45) 52 - Auto-zero cycle time (hours/12) 53 - Amount of zero drift (MV) 54- 55 - Max above setpoint allowed (ASU/20) 56 - COg switch time (seconds/20) 57 - Max sensor hold (seconds/60) 5B - Post inject recovery time (seconds/12) 59 - Inject contribution per second (3) 6x COg Scaling Base Point Parameters. 60 - (C02 Control 61 - Algorithm, Do Not 62 - Access) @- 6a 65- 66 67- 69 - 60- 35 - Max above setpoint allowed (ASU/20) 86 - Maximum sensor differential (ASW/40) 87 = Door bias (ASU/20) Examples: 1) Press "1" “ENT” key, the display should indicate either “12 hr" or “24 hr". To change, press any 9" key oF “0” key to return to original setting. Press "SEL" kay to zero command. 2) Press "11" “ENT” key, the display should indicate elther “tn-1" or “tn-2". This lets the tank switch ‘option to be enabled it purchased and installed. To change, press any “1-8” key or'0" to return to original setting. Press "SEL" key to zero command,x Functions ~ Chamber Configuration Function 0 — Exit Configuration Mode This function returns to Diagnostic Mode. No other entty is possible. Function 1 — Select 12 Hour Clock (On entry, the value display will show "12Hr" to “24Hr". In twelve hour mode, the time of day display has a decimal point to the right of the minute display Iit to indicate PM. Function 2— CO2 Enable (On entry, the value display will show “OFF” or "ON". If control is disabled, no setpoint may be entered Via the setup procedure, the tank select lights will be extinguished, and no Og control or inspection of the analog input will be permitted, Function 3 — Humidity Display Enable On entry, the value cispiay will show “OFF” or "ON". If display is disabled, no setpoint may be entered via the setup procedure. Function 4 ‘On entry, the value display will show “OFF or "ON". Ifthe alarm is disabled, no alarm will occur if the water pan is empty. ‘1x Functions — System Configuration Function 10 — Enable Second Chamber Support ‘On entry, the value display will show “Ch-1" or “Ch-2". Ifa single chamber mode is selected, the second ‘operator control panel, if present, will be disabled. If entry is being made from the second panel, it will ‘cease to operate after disabling the second chamber. Function 11 — Enable Dual CO Tank Support This function will enable or disable processing of a secondary CO2 supply tank. On entry, the value display will show "tn-1" or “tn-2", Function 12 — Select Default CO2 Tank ‘This function permits selection of the primary CO2 tank to be used in the event that the system is restarted. This selection Is normally made automatically by NICE during operation as tank switching occurs. No switching is performed when this action is made, Function 13 — Communication Enable This function enables or disables communication capability. On entry, the value display will show “OFF” or "ON", Function 14 — Select Communication Mode This function selects the communication driver and receiver to be used to communicate with the outside ‘word. Selection is made from “R.292" and "R.485", 2x Functions — System Parameters Function 20 — Automatic Select This parameter specifies the length of time the front panel will display the current parameter before advancing to the next. The unit must be in run mode to display the parameters in a round robin fashion ‘automatically. Function 21 — Sonic Alarm Timer Value ‘This parameter determines the length of time that the sonic alarm will sound if the "Alarm/Error" LED is liton the front panel. The value is given in seconds. If desired, entering 128 seconds wil initiate a ‘constant alarm until the mode switch is turned to standby/setup and back to RUN,If the alarm LED is activated, the sonic alarm will also be activated for the period specified. Depression of any keyboard key will silence the alarm until another activation condition is encountered. Function 22 — Communication Baud Rate Selection ‘This parameter specifies the baud rate (multiplied by 100) that communications are to run at. Valid numbers are 3, 6, 12, 24, 48 and 96, Any other entry will default to 1200 baud. Function 23 — Communication Address ‘This parameter specifies the address of this NICE unit on a multidrop communication link. If specified, the address must be contained in every communication command to the NICE system. Use of this identifier (a number between 0 and 92) permits multiple NICE systems to share the same RS-485 communication line Function 24 — Print Frequency ‘This parameter specifies the trequency (in minutes) that lines are to be printed on a status report. if the frequency is specified as zero, no report will be printed. Function 25 — Printer Page Length ‘This parameter specifies the length of a page on printed (on displayed) output. When a page is full NICE automatically advances the paper and prints headers on the new page. Function 26 — Chamber #1 Chart Recorder Parameter ‘This parameter specifies the output parameter of the chart recorder. "0" no output, "1" temp, and "3" humidity option, C02 Function 27 — Chamber #2 Chart Recorder Parameter This parameter specifies the output parameter of the chart recorder, and °3" humidity option, no output, “1” temp, "2" COZ Function 28 — Air Inject Time ‘This value specifies the time, in seconds, for an injection of air into the chamber. Operates for single or dual chamber systems. Each value specifies the control variable to be asserted on the chart recorder corresponding to the chamber. A value of zero selects no output, one selects chamber temperature, two selects CO percent, three selects humidity percent, Any other value is divided by four, the remainder is used to determine which of the four possible selections is desired. Function 29 — CO2 Time Out This value specifies the time, in minutes, for the CO2 percentage to achieve setpoint. If the COZ percentage doesn't achieve setpoint within the time period, the CO percent parameter red LED will ‘ light. This fault usually indicates low CO2 cylinder incoming pressure or an empty tank. ‘93x Functions — Temperature Control Parameters Function 30 (Temperature Control Algorithm, Do Not Access) Function 33, Function 34 Function 35 — Maximum Deviation Above Setpoint Allowed (MaxOver) ‘This value determines the maximum deviation, measured in analog significance units, that the chamber is permitted above the specified setpoint before an alarm condition is declared. An alatm conditions will cause the chamber to enter a safe condition where no power is enabled to any of the system output controls until the situation is rectified, Function 36 — Maximum Sensor Differential (MaxDiff) This value specifies a maximum differential, measured in analog significanice units, that any two ‘temperature sensors may deviate from one another or from the last red value. If this differential is ‘exceeded, a warning LED is shown on the running chamber. lf multiple sensors fall to read within the ‘specified limits, an alarm condition is declared. An alarm condition will cause the chamber to enter a safe Condition where no power is enabled to any of the system output controls until the situation is rectified.ax 5x Function 37 — Door Bias This value specifies in analog significance units (20 units equals 1°C), when added to the chamber temperature setpoint, defines the door setpoint. If the door is below its setpoint, its heater is enabled. If the door is above its setpoint, its heater is disabled Functions — Humidity Control Parameters Function 40 (Humidity Contro! Algorithm, Function 41 Do Not Access) Function 42 Function 43 Function 44 Function 45 — Maximum Deviation Above Setpoint Allowed (MaxOver) This value determines the maximum deviation, measured in analog significance units, that the chamber is permitted above the specified setpoint before an error condition is declared. Function 48 — RH Timeout (Minutes) This value specifies the time, in minutes, for the RH percentage to achieve setpoint. If the RH percentage doesn't achieve setpoint within this time period, the RH percent parameter red LED will light. This fault usually indicates low water in the water pan. Functions — CO2 Control Parameters Function 50 — Air Inject Time Before Zero This value determines the length of time the air inject valve is on to initiate the COz sensor auto-zero feature, Function 51 — Stabilization Time After Zero ‘This value determines the length of time the incubetor chamber and COz sensor is allowed to stabilize before resuming normal CO? control. Function §2 — Auto-Zero Cycle Time This value determines the length of time between the performance of the auto-zero function. Typically, the auto-zero function will occur at 12:00 AM and PM which has been determined to most effectively aulo-zero the IR Autoflow, Function 53 — Amount of Zero Drift This value indicates the amount of COp sensor drift that has occurred from the last COp sensor calibration, The amount of indicated drift willbe either positive or negative. The positive dri will indicate between (0 to 127 MV). The negative drift will indicate (256 — (0-127 MV). If elther the positive reaches 127 or the negative reaches 129 a COz sensor calibration is required, Function 54 — Do Not Access Function 55 — Maximum Deviation Above Setpoint Allowed (MaxOver) ‘This value determines the maximum deviation, measured in analog significance units, that the chamber 's permitted above the specified setpoint before an error condition Is declared, Function 56 ~ COz Switch Time Function 57 — Maximum Sensor Hold Time ‘This value determines the maximum time, in seconds, that a chamber may keep the COp sensor while the other chamber is requesting it tis functional only in dual chamber systems, Function 58 ~ Post Inject Recovery Delay ‘This value specifies the time, in seconds, for an injection of COp to be measurable at the sensor. When ‘C09 is injected into the chamber, the NICE system delays until this period has elapsed before making @ new control decision. In this manner tubing induced delays to not cause the COg system to overshoot the control setpoint.Function 59 — inject Contribution ‘These functions define the amount of CO2 introduced into the chamber for each second of valve activation, The amount is specified in analog significance units. 6x Functions — CO Range Control Parameters Function 60 (CO2 Control Algorithm, Function 65 Function 61 Do Not Access) Function 66 Function 62 Function 67 Function 63, Function 68 Function 64 Function 69 9.0 Calibration ‘The calibration of the IR Autoflow Is accomplished in three steps which are chamber temperature, door temperature and Cp sensor. The first two, chamber and door temperature should be completed anc stabilized before any Oz sensor calibration Is performed. Both chamber and door temperature calibration should be performed simultaneously with COp sensor calibration performed after the temperature is stabilized. Below, each calibration procedure is described in detail, For the best results, follow the procedure carefully, and if the desired result is not achieved, try procedure again from the start. on (Chamber Temperature Calibration ‘The IR Autoflow's TEMPERATURE CALIBRATION MUST BE PERFORMED WITHIN 1° OF THE PLANNED OPERATING TEMPERATURE. Normally, 37.0°C is the most common setpoint. To initiate the procedure, turn on the IR Autoflow via the power switch on the beck panel. Turn mode switch to standby setup to check the temperature value parameter for your planned operating temperature and change if necessary. Turn mode switch back to run and let stabilize for 6 to 12 hours. ‘At the beginning of this procedure, set a mercury glass thermometer in a glass beaker filled with water resting on a sheif in the middie of the IR Autofiow chamber. Do not place the glass beaker on the bottom of the chamber because it will result in a slightly higher temperature due to the heater i pen being located just below the chamber bottom. Placing the thermometer in glass beaker on the middle shelf will give the most accurate results for calibration, When the unit has stabilized at the operating temperature, perform the following calibration procedure, ‘¢ Turn mode switch to standby/setup. © Press “SEL” key to culture days, © Press “9734” "ENT" key, display blinks "FUNC 0"92 © Press "20" "ENT™ key, display blinks 20/Temperature (XX.X). (Enter in actual temperature trom thermometer XX.X), Press "XX.X" (actual ternperature) "ENT" key, displays new temperature. © Press "SEL" key to zero, display blinks “FUNC 0”. © Turn mode switch to run, The chamber temperature calibration is complete. Let unit stabilize for 8 to 12 hours. If the chamber temperature (actual thermometer still does not match the display, perform the above procedure again. In some cases it might be necessary to calibrate several times to achieve a calibrated stable condition Door Temperature Calibration ‘The IR Autotiow’s inner glass door temperature calibration is accomplished in two basic steps. Initial setup and running adjustment with humidity pan. The INITIAL TEMPERATURE CALIBRATION is accomplished similar to the chamber temperature ‘The actual running temperature is preprogrammed at 1°C above chamber temperature setpoint. So. ifthe chamber teriperature is set at 37.0°C the inner glass door will automatically run at 38.0°C. So, ‘when the calibration procedure is initiated, the temperature sensor calibration setpoint should be 1°C. above chamber temperature setpoint. For example, ifthe actual thermometer temperature was 365°C, Functions 20 would require an entry of that actual temperature. Function 21 would require an entry of 1°C above the actual thermometer temperature or 37.5°C. When the unit is stabilized at the operating temperature and the chamber temperature calibration has been completed, periorm the following calibration procedure: © Turn mode switch to standby/selup, © Press “SEL” key to culture days. © Press “9734” “ENT” key, display blinks “FUNC 0”. © Press “21" “ENT" key, display blinks 21/Temperature (XX.X). (Enter 19C above temperature from thermometer XX.X.) © Press “XX.X" (actual temperature +1°C) “ENT” key, displays new temperature, © Press “SEL” key to zero, display blinks "FUNC 0". © Turn mode switch to run, ‘The RUNNING ADJUSTMENT with humidity pan is performed to fine tune the door heater. Under ‘normal operating conditions, approximately 1 to 2 inches of condensation will form in corners of inner glass doors. If more than 2 inches of condensation in comers of inner glass door, perform the following: © Turn mode switch to standby/setup, "SEL" key to culture days. "9734" "ENT" key, display blinks “FUNC 0" “21" “ENT” key, dispiay blinks 21/Temperature (XX.X). (Enter 0.5°C lower than temperature displayed, ex: If display reads 375°C, enter 37.0°C.) © Press "XX.X" (cisplay temperature - 0.5°C) "ENT" key, displays new temperature, © Press "SEL" key to zero, display blinks "FUNC 0”. © Turn mode switch to run, Allow 4 hours to stabilize and check door condensation. Repeat procedure if more adjustment is required. lf major condensation is forming on inner wall of chamber, perform the following: © Turn mode switch to standby/setup, © Press "SEL" key to culture days, © Press "9734" "ENT" key, display blinks "FUNG 0" © Press "21" “ENT” key, display blinks 21/Temperature (XX.X). (Enter 0.5°C higher than temperature displayed, ex: If display reads 97.5°C, enter 38.0°C.)93 © Press “XX.X" (display temperature +0.5°C) “ENT” key, displays new temperature, © Press "SEL" key to zero, display blinks "FUNC 0°, ‘9 Turn mode switch to run Allow 4 hours to stabilize and check chamber wall condensation. Repeat procedure if more adjustment is required. C02 Calibration ‘The IR Autoflow infrared COg sensor has two calibration techniques performed, COg contro! and (C02 sensor calibration. The COg control procedure is a simple offset performed on the NICE front panel similar to the temperature offset requiring no tools. The COg sensor procedure Is more in depth requiring a few tools which takes approximately one half hour to perform, “The IR Autotow’s infrared COp sensor calibration must be performed at the planned operating temperature. Normally 37°C is the most common setpoint. Both calibration procedures require the use of a COp fyrite measuring instrument with @ 0 to 20 percent measuring range. Be sure to read the COp fyrite operating instructions for proper CO measurements 9.3.1 COg Control Calibration ‘The COz contro! calibration can be performed anytime the CO2 fyrlte measurement doesn't correlate to the NICE front panel CO2 percent display. However, it should be noted that this offset SHOULD NOT BE PERFORMED MORE THAN ONCE PER WEEK. If the COp fyrite measurement doesn't match the display aiter one week, within 0.3 percent (the tolerance of the fyrite), COp sensor calibration should be performed, ‘When unit is stabilized at the operation temperature and COz percentage, take a COp fyrite measurement and if necessary, perform the following calibration procedure. # Turn mode switeh to standby/setup. «© Pross "SEL" key fo culture days, «Press 794" “ENT” key, display blinks “FUNG 0” @ Press “24" “ENT” key, cisplay blinks 24/Busy or COz percent (XX) (Wait forthe display to indicate a GO percentage, then enter tyrte value) «# Enter °XX" (yite COg measurement “ENT key, displays new COg percentage. « Press “SEL” key to zero, display blinks "FUNC 0" ‘Turn mode ewitch to run. 9.3.2. CO2 Sensor Calibration ‘The CO2 sensor calibration should be performed approximately every year or when required. The ‘©0p sensor has two calibration points, a zero point (ambient COp percentage) and a span point (high COp percentage). Using a zero and span point, a curve is developed relating COp percentage ‘0 an output voltage 0-1 VDC (see chart 1). To calibrate the CO sensor, adjustments must be made at both ambient and high Cp percentage to have the voltage output remain consistent as it relates to the CO2 percent shown in the curve. ‘The tools required for this procedure are a medium size Phillips screwdriver, small blade screwdriver and a COz fytite instrument, The following calibration procedure should be performed ONLY after the chamber temperature is stabilized and the infrared CO sensor has been operating at the stabilized temperature for 2 hours, If the incubator has been in operation for a year or more, replacement of all the inline filters is necessary. After the above conditions are satisfied, proceed with the following: Step: Remove control center top panel (see drawing BCD-02528). NOTE It dual unit, only perform the following procedure on the left contro! panel and turn the mode switch ‘on the right control panel to standby/setup.Less Sey 88 ISIS D Coz CINCENTRATION #1 ATHOS Chart 4 ‘Output Voltage vs. CO2 Percent Step 2: Initiate COp sensor calibration function. © Turn mode switch to standby/setup. © Press “SEL” key to culture days. © Press "9734" “ENT” key, display blinks "FUNC 0”. © Press “2" “ENT” key, display blinks “door/COp" Open The IR Autotiows inner glass door for one minute to evacuate the chamber enviroment. Close the inner door and wait one minute to stabilize the chamber enviroment Step 3: Set COg sensor zero. © Press “ENT” key, display blinks zero and a positive or negative number. Locate the zero potentiometer (pot) on the COp sensor board located in the control center (see BCD- (02529, 30, 31). Using a small blade sorewdriver, adjust the zero pot to read 0 >: § on the display. 9 CONTROL,Step 4: Step 5: Step 6: Step 7: Set COg Sensor span © Press "ENT" key, display blinks SPAN/COz percent (X.X). Wait two minutes for the chamber to injact COp and lat the display COp percentage stabilize. Use a CO2 fyrite measurement instrument to measure the COg percentage via the sample port. Locate the span pot,'next to the zero pot. Using small blade screwdriver, adjust span pot to read the same as the COp fyrite measurement on the display, SPAN CONTROL} = = ole SS Open the IR Autotlow’s inner glass door for 30 seconds to evaluate the chamber environment. Close the inner glass and wait one minute to stabilize the chamber environment, © Press "SEL" key, display blinks "FUNC 0”. Turn mode switch, or if dual unit, switches to run. Let unit stabilize for one hour. Check (00g percent with CO2 fyrite. If COp fyrite measurement doesn't quite correlate to the NICE front panel display, perform COg control calibration to fine tune the COp calibration. ‘Once the COz sensor has been calibrated, reattach control center top panel (see drawing BCD-02528). 494 Low Sample Switch Adjustment “The low sample switch monitors the COp sensor pump system fiter to assure the proper sample flow through the filter. A quick check to verify the switch setting Is to plug the CO2 sensor pump system filter, located in the chamber, which should light the “low sample” LED on the front panel. Then, release the plug from the filter and the “iow sample” LED should not be ll. I the switch fails the above check, or the “low sample" LED periodically lights when the fier is known goad, perform the following adjustment: Step 1: Step 2: Step 3: Step 4: Step 5: Remove control center top panel (see drawing BCD-02528). Locate low sample switch. NOTE ‘Agjust switch — clockwise will tum on “low sample" LED, counterclockwise will turn off “low sample” LED. Plug the pump system filter, “low sample" LED should be it. Adjust, f necessary, clockwise till LED is I Release the pump system filter, “low sampl ‘counterclockwise tll LED is not lit. LED should not be lit. Adjust, if necessary, Check operation and reattach control center top panel (see drawing BCD-02528), USE SMALL BLADE SCREW DRIVER TO ADJUST SWITCH ae 1OW SRELE SWITCH [pe IR SENSOR10.0 Preventative Maintenance The IR Autofiow Automatic COp Incubator requires periodic preventative maintenance to insure a reliable controlled atmosphere, 40.1. Tubing Check all tubing connections for tight fit and brittleness. Replace if necessary. 102 CO Supply Filter ‘The CO2 Supply Filter should be replaced every fifth empty COz tank or when the filter is visibly discolored (yellow-brown). Note direction of flow (IN is labeled on one side of filter) when replacing filter. 10.3 Air Inlet Filter (Dry) ‘The Air inlet Filter is located on the backpanel, The purpose of the filter is to cleanse the room air which is drawn into the chamber, via the CO2 sensor sample pump, for three seconds every minute {0 assure the proper amount of oxygen is available to the cultures. The air inlet filter should be replaced every three months or when the fillers visibly discolored (yellow/brown). Note direction of flow (IN is labeled on one side of fiter) when replacing filter. 10.4 Infrared CO2 Sensor Pump System Filters (Wet) The Infrared COp Sensor Pump System Filters are located in the chamber and in the control center monitored by a pressure switch indicated on the front panel via the "LOW SAMPLE" LED. The purpose of the filters are fo cleanse the COz Sensor sample air. The filter can be autoclaved for sterility, but should be REPLACED EVERY SIX MONTHS, or when the "LOW SAMPLE" LED is lit "Note direction of flow (IN is labeled on one side of filter) when replacing the filter. 10.5 IR Autoflow Chamber ‘The chamber maintenance is only required as to the extent of cleanliness and sterility. The shelves and bracket supports are all removable and autoclavable. The interior should be wiped down with an appropriate disinfectant such as AMPHYL", ROCCAL Il, ISOPROPYL or equivalent. DO NOT USE ANY CHLORINATED OR HALOGEN MATERIALS IN THE CHAMBER, 10.6 IR Auloflow Water-Jacket ‘The water-jacket requires no anti-bacterial agents, The IR Autofiow already incorporates a copper tube producing copper sulfate which eliminates bacterial growth within the water-jacket. 00 NOT USE ANY CHLORINATED OR HALOGEN MATERIALS IN THE WATER-JACKET. 10.7 IR Autotiow Incubator Log In order to keep a record of the preventative maintenance schedule, use the IR Autoflow Incubator Log provided. Following the log will assure the proper maintenance schedule and thus increase the reliability of the incubator. For further assistance, call NuAire Service at 1-800-328-3362 or (612) 553-1270. “Rogistered trademark of National Laboratories Lehn & Fink Division, Montvale, NJ 07645,IR AUTOFLOW INCUBATOR LOG M2* TEMP CC) CHECK SEE (PYRITE) DAY AY Fivtens | CALIBRATE WEEK TUBING SENSOR COMMENTS:vex ct, 7— 3 amos ovau wus 6+—~|7a sans Torus itis / ain Tounoa 29 Az #00 ae aa sonrram 200 — pan ema zesongs Tmor we @ 71¥L30 sna oe a con > 0 &) OR | (" “42 Beh [Si as ' ie SS STL | coy geaos as |_o.ame 2 | |_— fides sain spans —| Ly a UAT Helo O° was AL =o TU ee | tera nn Ll) yh aes ff [-—oxvoe eosnas 203 [ [ae 2 | pia _ Gas Gow on oe a th i T = ij . an se | “hae ane is ee aoSe Snes TaN a ee TET Poses aes fo J—sns TET Tay OTS — ee ROTATES [osshas|41.0 Error Indicators and Troubleshooting Step 1: NOTE ALL ERROR INDICATORS. When the incubstor is running, any and all red LEDS indicate an error. ‘Step 2: CLEAR ERROR INDICATORS. Error indicators can be cleared by turning mode switch to standby! sotup and back fo run Step 3: MONITOR RE-OCCURRENCE OF ERROR INDICATORS. If re-occurrence of the error indicator immediate or daily, use guide below to correct the situation. Error Indicator Troubleshooting Guide CHAMBER TEMPERATURE @ CHAMBER TEMP Indicator: (3) Temperature sensors are not calibrated, Correction: Perform chamber temperature calibration (see section 9 1). © CHAMBER TEMP Indicator: Chamber over temp ALARM @ ERROR Correction: Perform chamber temperature calibration (see section 9.1). {not calibration, temp sensor or main contro} board fault Incubator Does Not Heat Correction: Check fuse on main control board inside control center. If not fuse, main contro! board fault (Og PERCENT NOTE: if the CO2 Percent indicator Is It, as shown below, the backup COz control system is in effect, Turn- ing the mode switch to standby/setup and back to run will clear the error indicator and allow the C02 control system to resume as normal, Ifthe error indicator re-occurs, make the appropriate cor- rection as state below @ COp PERCENT Indicator: COz Percent over setpoint by more than 1 percent. Correction for New Unit: Reduce CO gas cylinder regulator pressure. Correction for Existing Unit: Replace In-Line Filters and perform CO2 Sensor calibration (see section 9.3.2). @ C02 PERCENT Indicator: COg Percent does not reach setpoint. Correction for New Unit: Check CO2 gas supply to unit. Increase CO2 gas cylinder regulator pressure. Correction for Existing Unit: Replace In-Line Filters and perform GO2 ‘Sensor Calibration (see section 9.3.2) Incubator CO Percent does not Correction: Perform COz Control Calibration (see section 9.3.1) ‘match the Fyrite Instrument Rapidly Fluctuating COz Correction: Replace In-Line Filters and perform COz Sensor Percentage Calibration (see section 9.3.2), RH PERCENT @ RH PERCENT Indicator: AH Percent over or under setpoint. ‘Correction: Perform RH Percent Calibration (see operating instructions).— ea reas aan | San ‘pwso8 oaus0D 3010 7 J ewan] i (Sat ees or] saa neo 1 TOUTES alasGENERAL INDICATORS @ TIME OF DAY O ER:AC ER:AD ERW2 ERSU dely @ LOW WATER @ LOW SAMPLE ‘Audible Alarm with No Error Indicators Lit Indicator: Interruption of power to incubator. Correction: Turn mode switch to standby/setup and back to run. Indicator: Power to incubator is below 90 VAC. Indicator: Main control board fault. Correction: Replace main control board. Indicator: Fallure of 12 VDC source from power supply. Correction: Replace power supply. Indicator: Main control board falled to read correct setup information, Correction: If error signal is continuous, replace main control board. if error signai appears once, recalibrate incubator ‘temperature and COp control Indicator: CO2 Control is in delay for one of the following reasons: 1) Power interruption just occurred. Will resume CO2 Control within one half hour. ‘To bypass delay, perform following procedure: © Tum Mode Switch to Standby/Setup Press [i] to Indicate Red LED Next to Culture Days Press 9734’ [bs], Display Blinks FUNC 0° Press ‘24’ [&9], Display Blinks Function 24 and CO2 Percent or Busy Press (3, Display Blinks FUNC 0 Turn Mode Switch Back to Pun 2) Chamber temp is not within 2.0°C of setpoint. Cannot bypass this delay. Indicator: Low water in jacket Correction: Add water to jacket via fill port Indicator: Low chamber sample pressure to CO Sensor. orrestion: Replace In-Line Filters. f not corrected (see section 9.4) Indicator: Tank switch occurrence Correction: Turn mode switeh to standby/setup and back to run, For further assistance, call NuAire customer service at 1-800-328-3952 or (612) 583-1270.12.0 Replacement Parts List ‘The following is alist of replacement parts. Please consult NuAlre for parts not listed. To order, specity mode! no,, serial no,, and part no. Quantity NU-2600E- tem No. PartNumber Description NU-2500E _N2700E + A-2500-418 _NU-2600 Nice Main Gontrol Board 1 2 2800-417 NU-2600 Nice Main Control Board - 3 A-2500-417_NU-2700 Nice Main Control Board - 4 Aa8ep-1z3 Nee Hon Panel Bowe “=o op Jey fal 6 \B-2590-982- Nica TouchFanctteptions) 6-190 (20 AR 7 8 AS98615 Power Supply Assy FpS00- 24 8.990308 EM Filter 9 999729 Mode Switch 10 F-2500-212 Infrared COp Sensor Assy 44 A-999624° CO Control Valve (2 Way) 12 999678 Main Power Switch 43 A-999741 Feat Switch 14 A-999605-01 Sensor, Glass Door 15 A-998605-03 Sensor, Chamber 16 —-A-999605-03 Sensor, Water Jacket 17 &-980854 COz Regulator 18 999221 Circuit Breaker (5 amp) 19 999228 Circuit Breaker (30 amp) 20 A-1500-010 Magnetic Door Gasket 21 -F-2500:948 Door Pane! Heater 22 -A-1500-051 Glass Door 23 -F-1800-902 Heater Pan Assembly 24 A-1500-065.—_-Sholf Brackets (Pair) 25 A-980448 Leg Levelers 26 © A-999582 Electrical Cord 3. Cond. 2.4 meter 27 -A-980824 1/4" NPT x 8.5mm Tube Fitting (Nylon) 28 A-980308 Hose Barb (1/8" NPT x 3mm Tube) 29 © A-999654 Magnetic Switch 30 A-980464 Magnet 31 A-999599 Pressure Switch (Pump Sample) 198 Inch 32 A-980758 Drain Valve ‘A-999760 Safety Relay 34 A-999014 Fan 12 VDC. 35 -A-999314-01 Fuse (IR Sensor, 1 amp, 250 Volt) 86 A-999314-03 Fuse (Main Control Board, 3 amp, 250 Volt) 87 A-980386 In-Line Filter (Wet) Green Dot 38 A-980385 Inline Filter (Ory) 39 A-s99008 Pump Sample 40 -A-999622 Air Inlet Valve (3 Way) 41 A-999622 Pump inlet Valve 42 -A-999622 Pump Return Valve 43 -A-990844 MOV Suppressor 44 -A-999752 ‘Transformer (300 VA) 45 F-2500-361 Tube Heater Assy (Iniet) 46 -F-2500-382 Tube Heater Assy (Return) 47 F-2700-278 Tube Heater Assy (Side/Side) 48 2700-278 Tube Healer Assy (Over/Under) Pee ana Steere ge we eee Os ss oNNNNEBWNAszEuvons | sawwwavawssBuwss |i een Saad Ady LATedOeelTot ass [areca er Sonne ian ain TH TS “f Ec S- eo SF tis sole ltas