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Medical Officer KPJ Puteri Specialist Hospital No. 33, Jalan Tun Razak (Susur 5) Johor Bahru

The PCR test results for Noor Azizah binti Ab Hamid, a 49-year old female patient, detected COVID-19 in samples taken from her nasopharyngeal and oropharyngeal areas. The test detected the virus in three genes: the envelope gene, the nucleocapsid gene, and the RNA-dependent RNA polymerase gene. While the test can detect the virus, it cannot determine if it is viable or non-viable. The results were validated by a medical microbiologist and the test used was a multiplex real-time RT-PCR assay to simultaneously detect three target genes specific to COVID-19.

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53 views2 pages

Medical Officer KPJ Puteri Specialist Hospital No. 33, Jalan Tun Razak (Susur 5) Johor Bahru

The PCR test results for Noor Azizah binti Ab Hamid, a 49-year old female patient, detected COVID-19 in samples taken from her nasopharyngeal and oropharyngeal areas. The test detected the virus in three genes: the envelope gene, the nucleocapsid gene, and the RNA-dependent RNA polymerase gene. While the test can detect the virus, it cannot determine if it is viable or non-viable. The results were validated by a medical microbiologist and the test used was a multiplex real-time RT-PCR assay to simultaneously detect three target genes specific to COVID-19.

Uploaded by

Lynn Lynzz
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Dr.

Md Mohkseen
Medical Officer
KPJ PUTERI SPECIALIST HOSPITAL
No. 33, Jalan Tun Razak (Susur 5)
Johor Bahru

Patient Name : NOOR AZIZAH BINTI AB HAMID


Ward / Clinic : ACCIDENT & EMERGENCY Lab No. : 0921606739
Age : 49Y Sex : Female Date Received : 31/01/2021 10:41
IC No. : 720124-11-5324 MRN : 592944 Date Reported : 01/02/2021 20:53
DOB : 24/01/1972 Report Status : Final

Result Unit Reference range

PCR for 2019-NOVEL CORONAVIRUS, COVID-19/SARS-CoV-2 DETECTION


Sample type Nasopharyngeal and Oropharyngeal Swab
** Sarbecovirus Envelope, E gene Detected
(First run, ct value:33.47)
(Second run, ct value: 33.96)

** COVID-19 Nucleocapsid, N gene Detected


(First run, ct value:33.09)
(Second run, ct value: 33.09)

** COVID-19 RNA-dependent RNA polymerase, Detected


RdRp gene
(First run, ct value:33.42)
(Second run, ct value: 33.88)

** COVID-19/SARS-CoV-2 Viral RNA Detected

Note:
Covid-19 rRT-PCR assay unable to differentiate between viable and non-viable virus.
Please correlate clinically.

Methodology:
By Seegene Allplex 2019-nCoV (COVID-19/ SARS-CoV-2) Assay
This one-step, multiplex real-time, RT-PCR assay simultaneously detect and identify 3 target genes specific for COVID-19, which include the
Sarbecovirus envelope protein gene (E-gene), COVID-19 nucleocapsid protein gene (N-gene) and COVID-19 RNA-dependent RNA polymerase
gene (RdRp-gene).

This novel coronavirus, initially designated as 2019-nCoV that was first detected in Wuhan City, Hubei Province, China at the end of 2019. The
virus has been named as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by Coronavirus Study Group (CSG) of the
International Committee on Taxonomy of Viruses (ICTV) and the disease caused has been named by World Health Organization (WHO) as
Coronavirus Disease 2019 (COVID-19). A false negative result may occur if inadequate viral load is present in the specimen as a result of the
following reasons: Improper sample collection, or collection of sample at an early stage of illness, especially before the symptom onset, or
collection of sample at late stage of illness, especially after the symptom relief, or specimens was not handled and transport appropriately. RNA
viruses in particular show substantial genetic variability. Although efforts were made to design RT-PCR assays to conserved regions of the viral
genomes, variability resulting in mis-matches between the primers and probes and the target sequences can result in diminished assay
performance and possible false negative results. A single negative COVID-19 test (especially if from upper respiratory tract specimen), or a

Printed by Nurfazirah Binti Rahmat on 05/10/21 01:52:33 PM Page 1 of 2

Electronically Validated Report. No Signature Required.

Address:- 33, Jalan Tun Abdul Razak, ( Susur 5) , 80350 Johor Bahru, Johor Tel: 07-2233377 Fax: 07-2212805
Dr. Md Mohkseen
Medical Officer
KPJ PUTERI SPECIALIST HOSPITAL
No. 33, Jalan Tun Razak (Susur 5)
Johor Bahru

Patient Name : NOOR AZIZAH BINTI AB HAMID


Ward / Clinic : ACCIDENT & EMERGENCY Lab No. : 0921606739
Age : 49Y Sex : Female Date Received : 31/01/2021 10:41
IC No. : 720124-11-5324 MRN : 592944 Date Reported : 01/02/2021 20:53
DOB : 24/01/1972 Report Status : Final

Result Unit Reference range


positive test result for another respiratory pathogen result does not exclude COVID-19 infection.

If there is a strong clinical, and/or epidemiological suspicion of COVID-19 infection, the submission of a new specimen and request of Covid-19
testing is highly recommended. Expectorated sputum should be collected from patients with productive cough and the induction of sputum is not
recommended. A lower respiratory tract aspirate or bronchoalveolar lavage should be collected from patient who is intubated. A confirmed case
of Covid-19 is defined as a person with a positive Covid-19 RT-PCR. The symptoms and physical signs, stage or progress of disease,
epidemiological links, other laboratory examinations and therapeutic reactions of the patients should be comprehensively considered during their
clinical diagnosis and treatment. Commercial multiplex PCR assays for respiratory pathogens may detect endemic human coronaviruses
(HCoV), known to cause mild to moderate respiratory tract infections. These are the beta-coronaviruses HCoV-OC43 and HCoV-HKU1, and the
alpha-coronaviruses HCoV-229E and HCoV-NL63. It is important that positive results for these viruses should not be confused with COVID-19.

References:
1. World Health Organization. Novel Coronavirus (2019-nCoV) technical guidance. https://www.who.int/emergencies/diseases/novel-
coronavirus-2019/technical-guidance (Accessed on February 04, 2020).
2. Corman V, Bleicker T, Brünink S, Drosten C, Landt O, Koopmans M, et al. Diagnostic detection of Wuhan coronavirus 2019 by real-time
RTPCR 2020 [17 January 2020].
Available from: <https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf>

Validated by Dr. Muhammad Nazri Aziz (Medical Microbiologist) on 01/02/2021 8:53:20 PM

Consultant Pathologist:-
Dr. Muhammad Nazri Bin Aziz; MD (USM), MPath. Med. Microbiology (UKM), Consultant Medical Microbiologist

Printed by Nurfazirah Binti Rahmat on 05/10/21 01:52:33 PM Page 2 of 2

Electronically Validated Report. No Signature Required.

Address:- 33, Jalan Tun Abdul Razak, ( Susur 5) , 80350 Johor Bahru, Johor Tel: 07-2233377 Fax: 07-2212805

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