Scribd
Upload
578 views7 pages

Primary and Secondary Screening - Crowded Plate

Primary screening helps determine microorganisms and organic acids in cells, while secondary screening provides quantitative and qualitative information about cells or desired strains. The crowded plate technique is used for primary screening to identify microorganisms capable of producing antibiotics. It involves plating a suitable aliquot of serial dilutions to yield 300-400 colonies on an agar plate, called a crowded plate. While simple and fairly rapid, it still requires further analysis and may not indicate antibiotic production against a specific organism. The technique was applied in a study that developed a novel method for quantifying soil suppression of fungi using a modified crowded plate approach to obtain antagonistic microorganisms.

Uploaded by

Naevisweloveu
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
578 views7 pages

Primary and Secondary Screening - Crowded Plate

Primary screening helps determine microorganisms and organic acids in cells, while secondary screening provides quantitative and qualitative information about cells or desired strains. The crowded plate technique is used for primary screening to identify microorganisms capable of producing antibiotics. It involves plating a suitable aliquot of serial dilutions to yield 300-400 colonies on an agar plate, called a crowded plate. While simple and fairly rapid, it still requires further analysis and may not indicate antibiotic production against a specific organism. The technique was applied in a study that developed a novel method for quantifying soil suppression of fungi using a modified crowded plate approach to obtain antagonistic microorganisms.

Uploaded by

Naevisweloveu
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 7

Primary and Secondary

Screening
Crowded Plate Technique
Members:
Mearelle Kate Alfante, Sigred Evangelista
Glenn Royce Alba, Regie Japsay
Types of Screening
Primary screening helps to
determine the microorganisms
and organic acid in cells and
other desired organisms.

Secondary screening can be


used for determining the
quantitative and qualitative
information about the cells,
desired organisms or strains.
Crowded Plate Techniques

This technique of primary screening helps


to determine the microorganisms that are
capable of producing the antibiotics or
not.

A “suitable aliquot” of “serial dilution” is


chosen that can produce 300 to 400
colonies when it is plated on the agar
plate, which is also called a crowded
plate.
Source:Screening of industrially important microorganism - industrial microbiology.
BrainKart. (n.d.). Retrieved October 27, 2022, from
https://www.brainkart.com/article/Screening-of-Industrially-Important-
Microorganism_40998/
PROS CONS

Still requires further


Simple, fairly rapid
analysis

it will not give indication of


good technique for
antibiotic producing
obtaining microorganisms
organism against a desired
that can produce antibiotics
organism
Application of the Technique
Split-agar assay of antifungal soil microbial metabolites
by Aparna & Rao

Aparna, K., & Rao, D.L.N. (2016). Split-agar assay of antifungal soil microbial metabolites. Biocatalysis and Agricultural Biotechnology, 6, pp 184-188.
Highlights of the Research
Development of a novel method of quantifying the soil
suppressiveness of phytopathogenic fungi
Modified crowded plate technique was employed in obtaining
the antagonistic microorganisms
Thank you!

You might also like