)12( نموزج رقم
Diploma of Immunology ...... : أكاديمية/ جامعة
................................................................ : معهد/ كلية
..........................................................المعامل............. : قسم
توصيف مقرر دراسى
بيانات المقرر-1
2nd : المستوى/ الفرقة Immunology : اسم المقرر : الرمز الكودى
year التيرم االول
ساعة30 عملى ساعة30 نظرى: التيرم االول : عدد الوحدات الدراسية معامل: التخصص
1st term
) ساعات عملي أسبوعيا2 ساعات نظري و2 ( أسبوع مقسمة15 ساعة في60
: هدف المقرر-2
Provide basic information about the structure and function of
the immune system, mechanism of immunity and immune
mediated diseases as well as the different serological and
molecular methods used to diagnose different diseases
By the end of this course, the students SHOULD be able to : المستهدف من تدريس المقرر-3
A1= Describe the physiology of the immune system, its structure, المعلومات.ا
normal function. : والمفاهيم
A2- Identify beneficial and harmful immunological reactions.
A3- Recognize the principle of different serological techniques used
in diagnosis.
A4- Know the principle of different molecular techniques
A5- Know the laboratory method of Isolation of mononuclear cells
by density separation technique
A6- Recognize the fundamental aspect HLA (human leukocytic
antigens) and HLA typing by serological (lymphocytotoxicity) and
molecular genetic methods
A8- List different types of Electro-Immnodiffusion
B.1 Critically assesses laboratory results.
المهارات-ب
B.2 Understand the principle and operation of relevant laboratory
equipment. : الذهنية
B.3 Solve some common microbiologically related medical problems
and interpret results and reports of microbiological, serological,
immunological and molecular tests.
B.4 Able to select the suitable sample and the suitable laboratory
test for diagnosis.
B.5 Able to choose the required measurements for prevention and
controls
B6 - Judge the results of PCR technique.
B7- Understand the principle and operation of Electro-
Immnodiffusion
c1- Work safely in a medical laboratory. المهارات المهنية-ج
:الخاصة بالمقرر
c2- Ability to understand different methods of laboratory diagnosis.
c3- Solve some common microbiologically related medical problems
and interpret some antigen antibody or molecular tests.
c4- Perform some serological tests used for detection of viruses and
fungi in clinical samples and analyze the results.
المهارات-د
d1. Adopt the principles of lifelong learning needs of the medical : العامة
profession (continuous professional development; CPD).
d2. Use computers efficiently in reaching biomedical information to
remain current with advances in knowledge and practice.
d3. Present information clearly in written, electronic and verbal
forms.
d4. Apply English language as needed for appropriate learning and
communication in relation to medicine.
1st Term : محتوى المقرر-4
Electro-Immnodiffusion
a)Immunoelectrophoresis (IEP) 1st term
Serum electrophoresis: multiple myeloma,
Waldenstrom macroglobulinemia.
Urine protein electrophoresis.
CSF protein electrophoresis
b)Immunofixation
c)Rocket Electroimmunodiffusion (EID)
d)Counter immunoelectrophoresis (CIEP)
Types of antigen antibody reactions
Enzyme Linked Immunosorbent Assay (ELISA)
o Direct ELISA
o Indirect ELISA
o Competitive ELISA
o ELISA Troubleshooting
Radioimmunoassay (RIA)
Immunofluorescence
o Antinuclear antibodies
o Anti-dsDNA Antibody
o Anti-Smith Antibody
o Anti-Ro/SSA and Anti-La/SSB Antibodies
o Anti-Histone Antibodies
o Anti-LKM antibody
o Anti-neutrophil cytoplasmic antibodies
(ANCAs)
o Antiparietal Cells Antibodies
o Anti-smooth muscle antibody (ASMA)
o Anti-thyroid autoantibodies
o Anti-mitochondrial antibodies (AMA)
o Extractable Nuclear Antigens(ENA)
Isolation of mononuclear cells by density separation technique:
Ficoll-Paque principle
Troubleshooting of Ficoll-Paque
Flow cytometry
Immunoblotting
Southern blotting
Northern blotting
Western blotting
Biomolecules extraction
DNA extraction
RNA extraction
PCR Methods—Top Ten Strategies
Hot-start PC
Touchdown PCR
Nested PCR
Fast PCR
Direct PCR
GC-rich PCR
Multiplex PCR
Long PCR
Inverse PCR
Quantitative PCR
HLA (human leukocytic antigens)
HLA typing by serological (lymphocytotoxicity) and molecular genetic
methods
This course is scheduled for 3 hours per week أساايب التعليم والتعلم-5
(theoretical) and 6 hours per week (practical) in the
first term. The complete semester composed of 15
instructional weeks followed by one week of final
exam. Instructional methods will include:
1- Color plates.
2- PowerPoint (Data show) presentations.
3- Slide projector.
أساليب التعليم والتعلم للطالب ذوى-6
القدرات المحدودة
● Academic Lectures.
● Assignments.
Practical hands–on training
: تقويم الطالب-7
األساليب المستخدمة-أ
1. Practical examination
2. Oral examination
3. Written examination
a. Midterm exams التوقيت-ب
b. Final exam
عملي وشفوي%20 نظري و% 80 توزيع الدرجات-ج
: قائمة الكتب الدراسية والمراجع-8
مذكرات-أ
كتب ملزمة-ب
Medical Microbiology - 27E (2016) كتب مقترحة-ج
(DocToon.Page(
Review of Medical Microbiology and
Immunology, 14th Edition
Lippincott's Illustrated Reviews -
Immunology (Thao Doan, Roger
Melvold, 2017)
الخ...... دوريات علمية أو نشرات-د
Week Theory Practice
1st week Immunoelectrophoresis
(IEP)
Serum electrophoresis:
multiple myeloma,
Waldenstrom
macroglobulinemia.
Urine protein
electrophoresis.
CSF protein electrophoresis
2nd week Immunofixation -
3rd week Rocket
Electroimmunodiffusion (EID)
4th week Counter
immunoelectrophoresis
(CIEP)
5th week Enzyme Linked
Immunosorbent Assay
(ELISA)
Direct ELISA
Indirect ELISA
Competitive ELISA
ELISA Troubleshooting
6th week Radioimmunoassay(RIA)
7th week Immunofluorescence
o Antinuclear antibodies
o Anti-dsDNA Antibody
o Anti-Smith Antibody
o Anti-Ro/SSA and Anti-
La/SSB Antibodies
o Anti-Histone Antibodies
o Anti-LKM antibody
o Anti-neutrophil
cytoplasmic antibodies
8th week Immunofluorescence
Antiparietal Cells
Antibodies
Anti-smooth muscle
antibody (ASMA)
Anti-thyroid
autoantibodies
Anti-mitochondrial
antibodies (AMA)
Extractable Nuclear
Antigens(ENA)
9th week Isolation of mononuclear cells
by density separation
technique:
Ficoll-Paque principle
Troubleshooting of
Ficoll-Paque
Flow cytometry
10th week Immunoblotting
Southern blotting
Northern blotting
Western blotting
11th week Biomolecules extraction
DNA extraction
RNA extraction
12th week PCR Methods—Top Ten
Strategies
Hot-start PC
Touchdown PCR
Nested PCR
Fast PCR
Direct PCR
13th week GC-rich PCR
Multiplex PCR
Long PCR
Inverse PCR
Quantitative PCR
14th week HLA (human leukocytic
antigens)
15th week HLA typing by serological
(lymphocytotoxicity) and
molecular genetic methods
Prepared by:
Dr: Wageeh Salem Elnagy
Assistant Professor of Medical Microbiology and Immunology
&
Dr: Lamiaa Abdel Moned Adel
Professor of Medical Microbiology and Immunology
