SVKM’s Narsee Monjee Institute of Management Studies

Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, Mumbai

School of Pharmacy & Technology Management , Shirpur

Program: M.Pharm (All Specialisations) Semester : I

Course : Modern Pharmaceutical Analytical Techniques Code: MQA 101T

Teaching Scheme Evaluation Scheme
Term End Examinations
Lecture Practical Internal Continuous
(TEE)
(Hours per (Hours per Credit Assessment (ICA)
(Marks- 75 in Question
week) week) (Marks - 25 )
Paper)
4 0 4 Marks Scaled to 25 Marks Scaled to 75
Scope: This subject deals with various advanced analytical instrumental techniques for
identification, characterization and quantification of drugs. Instruments dealt are UV-Vis, IR,
NMR, Mass spectrometer, HPLC, GC etc.
Pre-requisite: Basic knowledge of analytical techniques
Objectives: The course is designed to provide detailed information about various advanced
analytical instrumental techniques for identification, characterization and quantification of
drugs.
Outcomes:
After completion of the course, students would be able to :
1. Explain the applications of various analytical techniques like UV, IR, NMR, Mass, X-Ray
Diffraction, Chromatographic and electrophoretic analysis for various Pharmaceutical
Products Analysis
2. Apply the various Analytical Techniques for various Drugs and Pharmaceutical
Analytical Applications
3. Summarize all the theoretical knowledge on various instrumental techniques available for
analysis of various dosage and pharmaceutical forms
4. Apply the knowledge learnt in developing new procedures for analytical testing of
various dosage forms
Detailed Syllabus: ( per session plan )

Unit Description Duration

in hours
1 a. UV-Visible spectroscopy: Introduction, Theory, Laws, Instrumentation 10
associated with UV-Visible spectroscopy, Choice of solvents and solvent
effect and Applications of UV-Visible spectroscopy, Difference/
Derivative spectroscopy.
b. IR spectroscopy: Theory, Modes of Molecular vibrations, Sample
handling, Instrumentation of Dispersive and Fourier -Transform IR
Spectrometer, Factors affecting vibrational frequencies and Applications
of IR spectroscopy, Data Interpretation.
c. Spectroflourimetry: Theory of Fluorescence, Factors affecting
fluorescence (Characterestics of drugs that can be analysed by
flourimetry), Quenchers, Instrumentation and Applications of
fluorescence spectrophotometer.
d. Flame emission spectroscopy and Atomic absorption spectroscopy:
Principle, Instrumentation, Interferences and Applications.
2 NMR spectroscopy: Quantum numbers and their role in NMR, Principle, 10
Instrumentation, Solvent requirement in NMR, Relaxation process, NMR
 SVKM’s Narsee Monjee Institute of Management Studies
Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, Mumbai
School of Pharmacy & Technology Management , Shirpur

signals in various compounds, Chemical shift, Factors influencing chemical

shift, Spin-Spin coupling, Coupling constant, Nuclear magnetic double
resonance, Brief outline of principles of FT-NMR and 13C NMR.
Applications of NMR spectroscopy.
3 Mass Spectroscopy: Principle, Theory, Instrumentation of Mass 10
Spectroscopy, Different types of ionization like electron impact, chemical,
field, FAB and MALDI, APCI, ESI, APPI Analysers of Quadrupole and
Time of Flight, Mass fragmentation and its rules, Meta stable ions, Isotopic
peaks and Applications of Mass spectroscopy.
4 Chromatography: Principle, apparatus, instrumentation, chromatographic 10
parameters, factors affecting resolution, isolation of drug from excipients,
data interpretation and applications of the following:
 Thin Layer chromatography
 High Performance Thin Layer Chromatography
 Ion exchange chromatography
 Column chromatography
 Gas chromatography
 High Performance Liquid chromatography
 Ultra High Performance Liquid chromatography
 Affinity chromatography
 Gel Chromatography
Hyphenated techniques-LC-MS, LC-MS/MS, GC-MS, HPTLC-MS.

5 a. Electrophoresis: Principle, Instrumentation, Working conditions, factors 10

affecting separation and applications of the following:
 Paper electrophoresis
 Gel electrophoresis
 Capillary electrophoresis
 Zone electrophoresis
 Moving boundary electrophoresis
 Iso electric focusing
b. X ray Crystallography: Production of X rays, Different X ray methods,
Bragg‘s law, Rotating crystal technique, X ray powder technique, Types
of crystals and applications of X-ray diffraction.
6 a) Potentiometry: Principle, working, Ion selective Electrodes and 10
Application of potentiometry.
b) Thermal Techniques: Principle, thermal transitions and Instrumentation
(Heat flux and power-compensation and designs), Modulated DSC,
Hyper DSC, experimental parameters (sample preparation,
experimental conditions, calibration, heating and cooling rates,
resolution, source of errors) and their influence, advantage and
disadvantages, pharmaceutical applications. Differential Thermal
Analysis (DTA): Principle, instrumentation and advantage and
disadvantages, pharmaceutical applications, derivative differential
thermal analysis (DDTA). TGA: Principle, instrumentation, factors
affecting results, advantage and disadvantages, pharmaceutical
 SVKM’s Narsee Monjee Institute of Management Studies
Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, Mumbai
School of Pharmacy & Technology Management , Shirpur

applications.
Immunological Assays:
Radioimmunology assay (RIA), ELISA (Theory & practical) and knowledge
on Bioluminescence assays.
Total 60

Text Books:
1. Principles of Instrumental Analysis - Doglas A Skoog, F. James Holler, Timothy A.
Nieman, 5th edition, Eastern press, Bangalore, 2014.
2. Quantitative Analysis of Drugs in Pharmaceutical formulation - P D Sethi, 3rd Edition, CBS
Publishers, New Delhi, 2008.
3. Practical Pharmaceutical Chemistry – Beckett and Stenlake, Vol II, 4th edition, CBS Publishers,
New Delhi, 2007.
4. Spectrometric identification of organic compounds - Robert M Silverstein, 6th edition, John
Wiley & Sons, 2016.
Reference Books:
1. Spectroscopy of Organic Compounds, 2nd edition, P.S/Kalsi, Wiley estern Ltd., Delhi.2016.
2. Donald L Pavia, Gary M Lampman, George S Kriz., James R Vyvyan. Introduction to Spectroscopy,
5th Edition, Sengage Learning Publisher 2015.
3. Instrumental methods of analysis – Willards, 7th edition, CBS publishers. 2007.
4. Organic Spectroscopy - William Kemp, 3rd edition, ELBS, 2007.
Any other information : --

Total Marks of Internal Continuous Assessment (ICA) :25 Marks

Distribution of ICA Marks :

Description of ICA Marks
Sessional Examination 15
Term Work 10
Total Marks : 25

____________ ____________
Signature Signature
Dr. Amisha Vora (Approved by)
Section Head, Dr.Bala Prabhakar
Pharmaceutical Chemistry Dean, SPPSPTM