GC-MS/MS User Charges
1. GC-MS/MS (Triple Quadrupole- TQ8050). External users must pay extra 18% GST
NIPGR, NCR-Cluster Academic, Research Private Organizations Important user
Institutes Institutions and Start- information
ups
1. GC-MS (Scan Mode): 1. GC-MS (Scan Mode): 1. GC-MS (Scan Mode):
Untargeted - 450 INR 1.1.1) Untargeted - 1000 1.1.2) Untargeted - 2500 Output to user of GC-
per sample INR per sample INR per sample MS = peak separation
profile + RT + Area+
Base peak
Raw data processing 1.2.1) Raw data 1.2.2) Raw data
assistance- 1000 INR for processing assistance- processing assistance-
untargeted metabolomic data 2000 INR 4000 INR
analysis including library
search guidance, methods of
peak selection/filtering and
normalized data generation.
Targeted (Scan mode/ 1.3.1) Targeted (Scan 1.3.2) Targeted (Scan Relative quantification
relative quantification) mode/ relative mode/ relative based on external
quantification) quantification) standard
450 INR per sample 1000 INR per sample 2500 INR per sample
GC-TQ-MS/MS (SIM GC-TQ-MS/MS (SIM GC-TQ-MS/MS (SIM Output to user of GC-
Mode): Mode): Mode): MS/MS =
1.4) Targeted - 2000 INR per 1.4.1)Targeted - 5000 1.4.2) Targeted - 10000 Absolute
sample + 100 INR per INR per sample + 200 INR per sample + 1000 quantification based
precursor ion MS/MS (i.e. INR MS/MS analysis (per INR MS/MS analysis (per on authentic standards,
per metabolite) precursor ion) precursor ion) precursor ion and
reference ion
LC-MS/MS User Charges
Proposed User Charges for UHPLC-MS/MS (QTRAP 6500). External users must pay 18% GST
NIPGR, NCR-Cluster Academic, Research Private Organizations Important user
Institutes Institutions and Start-ups information
Phytohormone estimation:
2.1) Defense 2.1.1) Defense 2.1.2) Defense Uses a mix of labelled
phytohormones (JA, JA- phytohormones (JA, JA-Ile, phytohormones (JA, JA- internal standard for
Ile, cis-OPDA, SA, cis-OPDA, SA, ABA): Ile, cis-OPDA, SA, each sample provided
ABA): ABA): by the facility
2000 INR per sample + 4000 INR per sample + 100 10000 INR per sample +
100 INR per precursor ion INR per precursor ion 500 INR per precursor
MS/MS (i.e. per MS/MS ion MS/MS
phytohormone)
Total charge for all five Total charge for five defense Total charge for five
defense phytohormones phytohormones per sample: defense phytohormones
per sample: 2500 INR 4500 INR per sample: 12500 INR
2. 2) Growth 2.2.1) Growth 2.2.2) Growth
phytohormones [IAA phytohormones [IAA phytohormones [IAA Uses a mix of labelled
(Auxin), trans-zeatin (Auxin), trans-zeatin (Auxin), trans-zeatin internal standard for
(Cytokinin)] (Cytokinin)] (Cytokinin)] each sample provided
by the facility
2300 INR per sample + 10000 INR per sample +
100 INR per precursor ion 4600 INR per sample + 200 500 INR per precursor
MS/MS (i.e. per INR per precursor ion ion MS/MS (i.e. per
phytohormone) MS/MS (i.e. per phytohormone)
phytohormone)
SPE column for sample
Total charge for two Total charge for two preparation will be
growth phytohormones Total charge for two growth growth phytohormones provided
per sample: 2500 INR phytohormones per sample: per sample: 11000 INR
5000 INR
.
2.3) Amino acids 2.3.1) Amino acids 2.3.2) Amino acids Uses a mix of labelled
internal standard for
each sample provided
2000 INR/sample (UPLC- 5000 INR/sample (UHPLC- 10000 INR/sample by the facility
MS/MS charge) MS charge)
Amino acids in internal
standard are: Ala, Ser,
Pro, Thr, Ile, Asp, Glu,
Met, His, Phe, Arg,
Tyr, Trp, Asn, Gln,
Lys.
External calibration
curve made for
2.4) Flavonoids (based on 2.4.1). Flavonoids 4. Flavonoids following Flavanoids:
external calibration curve) Naringenin, Quercetin
Rutin, Myricetin
600 INR/sample 1500 INR/sample 3000 INR/sample Kaempferol
Epicatechin gallate
Epigallocatechin
Catechin, Cyanidin
Delphinidin,
Pelargonidin
Petunidin, Peonidin
Malvidin
2.5) Other targeted 2.5.1) Other targeted 5. Other targeted The users must have a
metabolite quantitation metabolite quantitation metabolite quantitation published method,
column and standards
2000 INR/sample (UPLC- 5000 INR/sample (UHPLC- 10000 INR/sample
MS/MS charge) + 100 MS/MS charge) + 200 INR (UHPLC-MS charge) +
INR per precursor ion per precursor ion 1000 INR per precursor
MS/MS (i.e. per ion
metabolite)
Total charge per Total charge per metabolite Total charge per
metabolite per sample: per sample: 5200 INR metabolite per sample:
2100 INR 11000 INR
External users can sent
students to do sample
preparation in the facility
External users can also send
lyophilized powder of plant
tissue (25mg) for defense
phytohormone sample
preparation if required
2.5.6) Sample preparation
charge: 2000INR per sample
for defense hormone and
aminoacids.
Growth hormone sample
preparation will not be
provided.
NOTES
The users are requested to contact the metabolome facility in-case of queries regarding price slot and
analysis to be used. Email of metabolome facility: [email protected]
The samples will be accepted only after the indents are processed by NIPGR. The users may give indentto
Metabolome staff (008) and the indents after signature from Metabolomics facility in-charge are sent to
Administration. The indents are further processed as normal consumable indents. Once the indents are
approved, the user can give the samples to the facility.
Only after indents are approved by purchase committee/ performa invoices are generated and payed (for
external users) can the user start sample preparation or send samples
Samples can be provided to Metabolome Staff after the indents are approved
Samples submitted in person or via post, without proper communication and prior approval by the
competent authority, will not be entertained. Payments processed regarding the same, without prior
approval of the competent authority, will not be the direct responsibility of Metabolome facility, NIPGR.
Sample list in Excel sheet must be provided to the facility with weight of samples and the sample tubes
must have similar codes for ease of handling (Lab name- day and month- sample number. Eg. JV-2611-01).
Average processing time after sample submission is 3 weeks for metabolites in price list, for other user
specific metabolites with a known method it is 6-8 weeks. The sample processing time starts after the indent
is approved by NIPGR and due verification of submitted samples.
Equipments required for sample preparation will be provided by the facility. Equipments for sample
preparation will be handled by the user with the guidance of the Facility-in-Charge/Authorized Operator
without any extra charges
User will be liable for any damage of the equipments by improper handling during sample preparation and
will be liable for compensation for any major damage (i.e. which will hamper equipment operation).
All the samples and standards must be prepared by using MS grade solvents for good quality result.
After analysis the raw data will be provided without any statistical processing.
Equipments required for sample preparation will be provided by the facility. Equipments for sample
preparation will be handled by the user with the guidance of authorized Operator without any extra charges.
User will be liable for any damage of the equipments by improper handling during sample preparation and
will be liable for compensation for any major damage (i.e. which will hamper equipment operation).
Facility will not be responsible for data processing/experimental result.
Kindly acknowledge the metabolome facility in your publications
For External users, the payment has to be done in advance (online transfer) to the Institute.
The payment will be done in the following bank detail for external users:
Accounts Holder’s Name: DIRECTOR, NIPGR
Bank Name and its address: State Bank of India, Jawaharlal Nehru University, New Delhi
Account Number (Saving account): 10596550290
IFSC code: SBIN0001624
Email of the Beneficiary: [email protected]
