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Hema311lab (W7

The document discusses hematocrit determination, which is the percentage of red blood cells in blood. It describes sources of error in measurement and lists the materials and steps for macrohematocrit and microhematocrit methods. Normal hematocrit reference ranges are provided for males and females.

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Aira Cortez
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33 views1 page

Hema311lab (W7

The document discusses hematocrit determination, which is the percentage of red blood cells in blood. It describes sources of error in measurement and lists the materials and steps for macrohematocrit and microhematocrit methods. Normal hematocrit reference ranges are provided for males and females.

Uploaded by

Aira Cortez
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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HEMATOLOGY (LAB)

Our Lady of Fatima University Laguna| BSMLS | 3-YA-2


Topic: Hematocrit Determination
HEMATOCRIT SOURCES OF ERROR
▪ AKA Packed Red Cell Volume (PCV) is the relative FALSE INCREASE
percent volume of red blood cell in a given portion → Under centrifugation
of blood. → Prolonged standing (10 mins)
▪ Expressed as % v/v → Inclusion of buffy coat
▪ PRINCIPLE: A whole blood is spun to separate its
component; the percent amount of red cell is then FALSE DECREASE
measured. → Over centrifugation
→ Improper sealing
METHODS OF MEASUREMENT → Hemolyzed specimen
• Macrohematocrit (Wintrobe method) → Increased concentration of anticoagulation
• Microhematocrit (Clay-Adam’s Method) → Underfilling the capilet

• REFERENCE RANGE: Male – 40 to 50% | Female – PATHOLOGIC CONDITIONS:


35 to 45% → Sickle cell anemia, thalassemia, macrocytosis, blood
loss, dehydration, hemoconcentration
MACROHEMATOCRIT METHOD
MATERIALS NEEDED RED BLOOD CELL INDICES:
• Wintrobe tube • Hematologic parameters whose computed values
• Test tube are used for differential diagnosis of anemia.
• Cotton/Gauze pad • Mean Cell Hemoglobin, Mean Cell Hemoglobin
• Centrifuge Concentration, and Mean Cell Volume
• EDTA blood sample

MICROHEMATOCRIT METHOD
MATERIALS NEEDED
• Lancet
• Capillary tube (Red)
• Cotton
• Clay seal
• Wax
• Microhematocrit centrifuge
• Microhematocrit reader

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