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Lelm216 1

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55 views2 pages

Lelm216 1

Uploaded by

gaikwaddevansh4
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Investigatory Project 1

Aim: To study the effect of pH on seed germination

Principle: pH is one of the most important factors that controls the composition of flora and
fauna in different terrestrial and aquatic ecosystems. pH of soils is essentially controlled by the
amount and type of various minerals and also by the quality and quantity of humus (dead,
decaying organic matter) present in it.
Seed germination is controlled by pH of the germinating medium. Seeds of different species
prefer a specific range of pH for maximum germination. pH not only controls the germination
of seeds but also growth and development, reproduction and various other metabolic activities,
of the plant.

Objectives: After completing the project, the students will be able to


1. Plan out an experiment and understand the use of appropriate chemicals, apparatus and
equipment, and learn the preparation of solutions.
2. Understand research methodology.
3. Generate, analyse and interpret the data and draw conclusions.
4. Conceive and choose other different themes related to pH and plant growth.
Materials required:
1. 125 seeds each of sunflower, mustard, green moong, alfa alfa, fenugreek and barley (selection
of seeds of different species may be made as per their availability)
2. Phosphate buffers
3. Distilled water
4. Petridishes of 7.5 cm diameter (15 pairs)
5. Blotting papers cut into circular discs to the size of petridishes

Procedure
(i) Prepare a range of pH buffers using Na2HPO4 and KH2PO4.
(ii) Wash the seeds with water and blot them dry.
(iii) Select an appropriate place in the laboratory where there is sufficient
light. Arrange petridishes in three horizontal lines, with 5 dishes in each
line. Arrange petridishes horizontally in three rows A, B and C with seven
dishes in each row.
INVESTIGATORY PROJECT 1

(iv) Place one blotting paper disc in each petridish.


(v) Wet the blotting papers with small quantities of buffer solution of 4.0
pH for petridish No. 1, 5.0 pH for petridish No. 2 and so on till all the
blotting papers in petridishes No. 1–5 in row 'A' are wet with appropriate
buffer solution.
(vi) Repeat the process for rows B and C also.
(vii) Spread selected seeds in each of the five petridishes in such a way that
each petridish contains 25 seeds in row 'A'.
(viii) Repeat the process for rows B and C using two other types of seeds.
(ix) Cover the petridishes and record your observation after every 24 hours
for 7 days. Tabulate your results as given.

Observation
Observe the emergence of radicle as an indicator of germination and record in
the table. Calculate the percentage of germination every day.

Table: Percentage of ................ seed germination


No and % of seed germination Total % of seed
germination
pH 1 2 3 4 5 6 7 A B C
4
5
6
7
8

Use the data of the table for graphic presentation.

Inferences and conclusion


The inferences and conclusion may be drawn on the basis of observations and the points given
below
• Find out at what pH range seeds of different species had maximum % of germination.
• Find out at what pH seeds failed to germinate or showed minimum % of germination.
• Is there any general pattern of seed germination with regard to pH ranges?
• What are the common features exhibited by the various types of seeds under varied pH
ranges? For example, did all the type of seeds show maximum germination in acidic range or
alkaline range or did pH preference varied between acidic and alkaline ranges?
• Did you observe any relationship among time period, seed germination and pH range?

101

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