HIGHER SECONDARY PRACTICAL EXAMINATION
BOTANY PRACTICAL KEY (PBTA)
HSE – II Time: 1 ½ hrs
Total score: 20
Q1. Prepare a T.S of the given specimen ‘A’ and identify giving reasons. Draw the ground plan
and label any two parts. Leave the preparation for valuation. 6 marks
Preparation – 2 Labelled diagram – 1½ Reasons – 2 Identification – ½
(Dicot stem / Monocot stem / Monocot root)
DICOT STEM
Identification features:-
Vascular bundles are conjoint and collateral.
Xylem is endarch.
Hence it is a stem.
Vascular bundles are limited in number.
Vascular bundles are arranged in a ring manner.
Vascular bundles are open.
Hence it is a dicot stem
The given specimen is Dicot Stem.
MONOCOT STEM
Identification features:-
Vascular bundles are conjoint and collateral.
Xylem is endarch.
Hence it is a stem.
Vascular bundles are numerous in number
and scattered in ground tissue.
Vascular bundles are closed.
Xylem is arranged in ‘Y’ shape or ‘V’ shape.
Hence it is a monocot stem
The given specimen is Monocot Stem.
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MONOCOT ROOT
Identification features:-
Vascular bundles are radial.
Xylem is exarch.
Root hairs are present.
Hence it is a root.
Vascular bundles are numerous in number.
Pith is large.
Xylem is round in shape.
Hence it is a monocot root.
The given specimen is Monocot Root.
Q2. Identify the materials ‘B’ and ‘C’ at sight by giving reasons. 2 marks
Reasons – ½ x 2 = 1 Identification – ½ x 2 = 1
B – Microscopic slides – Oscillatoria, Rhizopus, Spirogyra, Moss Protonema, Fern Prothallus
– Macroscopic specimens – Agaricus, Sargassum, Funaria gametophyte with sporophyte,
Nephrolepis sporophyte, Pinus Female cone, Pinus male cone
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The given material is Rhizopus with The given material is Oscillatoria
Sporangia (asexual reproduction). Vegetative filaments.
Identification features:- Identification features:-
The fungal hyphae are highly
B branched.
Hyphae have rhizoids,
Oscillatoria is simple filamentous,
unbranched, prokaryotic blue green
algae.
sporangiophore and sporangium Necridium is present.
bearing spores.
The given material is Spirogyra vegetative The given material is Moss Protonema.
filament. Identification features:-
Identification features:- It is gametophyte of Bryophyte.
Spirogyra is filamentous fresh water It is creeping, green, branched & a
green algae. filamentous. Develops from spores.
It has ribbon shaped spirally arranged
chloroplast.
The given material is Fern Prothallus.
Identification features:-
It is gametophyte of Pteridophyte.
Small, thalloid and heart shaped.
It bears sex organs.
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The given material is Agaricus basidiocarp. The given material is Sargassum.
Identification features:- Identification features:-
Fruiting body is called basidiocarp with Erect and branched thallus.
basidiospores. Thallus is differentiated into holdfast,
frond and stipe.
The given material is Funaria gametophyte The given material is Nephrolepis
with sporophyte. sporophyte.
Identification features:- Identification features:-
Plant body is differentiated into Sporophytic plant body is
rhizoids, stem like and leaf like parts. differentiated into root, stem & leaves.
Sporophyte is differentiated into foot, Spores are produced on sporophylls.
seta and capsule.
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The given material is Pinus female The given material is Pinus male
cone. cone.
It is large in size and has It is small in size and has
megasporophylls. microsporophylls.
C. Photograph of Bioreactor / Bt cotton / Cloning vector pBR322
Bioreactor – Bt cotton – Cloning vector pBR322 –
Bioreactors are large vessels It is genetically It is a plasmid cloning
which are used to produce modified cotton.
specific proteins or Contains cry genes vector.
enzymes. from bacteria Bacillus Used to transfer desired
Raw materials are converted thuringiensis. foreign gene into host.
into products by cells.
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Q3. Identify the given stage ‘D’ of mitosis and give reasons. 1 mark
Identification – ½ Reasons – ½
Metaphase Anaphase
Chromosomes are arranged at the Centromere split.
equatorial plate of the cell. Daughter chromosomes separate and
Spindle fibres are attached at the move to opposite poles of the cell.
centromere of chromosomes.
Q4. Write the aim of the experiment ‘E’. Draw and label the parts. 1½ marks
Labelled diagram – 1 Aim – ½
(a) Hydrilla Experiment –
Aim - To study evolution of oxygen during photosynthesis by Hydrilla experiment
(b) Paper chromatography
Aim – To separate the leaf photosynthetic pigments by paper chromatography.
(c) Study the distribution of stomata
Aim – To study the distribution of stomata on upper and lower surface of leaves.
(d) Yeast fermentation and production of alcohol
Aim – To demonstrate fermentation by yeast.
Hydrilla experiment Paper chromatography
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Yeast fermentation Stomatal distribution
Q5. Observe the given flower ‘F’. Describe its floral whorl calyx / corolla / gynoecium
using three technical terms.
(Three technical terms) Score 1½
Family Solanaceae
Calyx – 5 sepals, gamosepalous, valvate aestivation
Corolla – 5 petals, gamopetalous, valvate aestivation
Gynoecium – bicarpellary, syncarpous, superior ovary, axile placentation
Q6. Write down the ecological interaction of the specimen ‘G’. 2 marks
Name of the interaction – 1 Description - 1
Lichen Vanda / Loranthus
Name of the interaction – Name of the interaction –
Mutualism (+ +) Commensalism (+ 0).
Both organisms are benefitted. One organism is benefitted and the
Lichen is a symbiotic association other is neither benefitted nor
between fungi and algae. harmed.
Vanda is an Epiphyte.
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Cuscuta
Name of the interaction – Parasitism (+ -).
One organism is benefitted and the other
is harmed.
Cuscuta is a total stem parasite.
Q7. Prepare a C.S. of the given specimen ‘H’. Draw diagram and label any two parts. Leave
the preparation for valuation. 3 marks
Preparation – 1 Labeled diagram – 2
Q8. Viva voice 1 mark
Q9. Record 2 marks
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