EXPERIMENT NO.
4
Egg’s Osmosis
EGG’S OSMOSIS
INTRODUCTION:
From your experience in the kitchen, you might know what happens to an egg when you place it into
boiling water or food coloring. However, you probably never followed a recipe that instructed you to leave
an egg sitting overnight in a solution. You have a good reason to do just that in this experiment. If you
submerge an egg in different solutions for 24 hours, you can observe how substances enter and leave the egg.
The outcome may not be as tasty as your previous activities with eggs, but the results certainly will give you
food for thought.
What effect does the type of solution that an egg is submerged in have on the flow of materials into
and out of it? Let’s take several different household solutions and see what changes occur when you leave an
egg submerged in it for a day.
Materials:
6 pcs of 300 mL jar – c/o student 200 mL distilled water – c/o student
Graduated cylinder 20 g iodized salt – c/o student
Raw white eggs (shell intact) – c/o student Watch glass
800 mL vinegar – c/o student Triple beam or weighing scale
200 mL corn syrup – c/o student Weighing paper or filter paper
200 mL tap water
Hypothesis:
Predict what will happen when an egg is submerged for 24 hrs. in each of four different solutions (vinegar, salt
water, corn syrup, and tap water). Include four separate hypotheses, one for each solution type.
1. ______________________________________________________________________________
2. ______________________________________________________________________________
3. ______________________________________________________________________________
4. ______________________________________________________________________________
General Biology 1- Laboratory
Science, Technology, Engineering, and Mathematics
EXPERIMENT NO. 4
Egg’s Osmosis
Procedure:
Part One: Vinegar
1. Place a dish on the balance and tare. Carefully place the egg in the dish. Record the egg’s mass on the
chart.
2. Fill the graduated cylinder with 200 mL of vinegar and pour it into a jar. Carefully place the egg into
the beaker. Stay this set up for 24 hours.
3. Observe and record initial observations about the egg on the chart.
4. Carefully remove the egg from the vinegar. Observe any changes to the egg on the chart. Wash the
egg carefully. Remove the white eggshells.
5. Place the dish on the balance and tare. Then place the egg on the dish to get the new mass of the egg.
Record today’s new egg mass on the chart.
6. Repeat this in all 3 other eggs.
Part Two: Salted water
1. On a clean beaker, add 200 mL of water to the beaker.
2. Place weighing paper on the balance and tare. Next, weigh out 20 g of salt add to the beaker, and stir.
Carefully place the egg (removed eggshells; semi-permeable membrane left) into the beaker. Stay this
set up for 24 hours.
3. Repeat step 5 of part 1.
Part Three: Corn Syrup
1. Use an empty and clean beaker. Add 200 mL of corn syrup to the beaker. Carefully place the egg
(removed eggshells; semi-permeable membrane left) into the beaker. Stay this set up for 24 hours.
2. Repeat step 5 of part 1.
Part Four: Tap water
1. Use an empty and clean beaker. Add 200 mL of tap water to the beaker. Carefully place the egg
(removed eggshells; semi-permeable membrane left) into the beaker. Stay this set up for 24 hours.
2. Repeat step 5 of part 1.
General Biology 1- Laboratory
Science, Technology, Engineering, and Mathematics
EXPERIMENT NO. 4
Egg’s Osmosis
Group name: _______________________ Date: __________________________
Member’s name: Task:
PRE-LABORATORY ACTIVITY:
I. Safety Measures:
a. ______________________________________________________________________
b. ______________________________________________________________________
c. ______________________________________________________________________
II. Results
A. Insert pictures of the eggs under different types of solution.
TYPES OF SOLUTION
Egg soaked in vinegar Egg soaked in salted water
General Biology 1- Laboratory
Science, Technology, Engineering, and Mathematics
EXPERIMENT NO. 4
Egg’s Osmosis
Egg soaked in corn syrup Egg soaked in tap water
B. Observations:
Egg’s Initial Egg’s Mass after 24 Observations
Vinegar
Mass (g) hours (g) (initial and after 24 hrs.)
Initial:
Egg 1
After 24 hours:
Initial:
Egg 2
After 24 hours:
Initial:
Egg 3
After 24 hours:
Solution Egg’s Initial Egg’s Mass after 24 Observations
Type Mass (g) hours (g) (initial and after 24 hrs.)
Initial:
Salt water
After 24 hours:
Initial:
Corn syrup
After 24 hours:
Initial:
Tap water
After 24 hours:
General Biology 1- Laboratory
Science, Technology, Engineering, and Mathematics
EXPERIMENT NO. 4
Egg’s Osmosis
GUIDE QUESTIONS:
1. When the egg was first placed in vinegar, bubbles began appearing around it. What can be inferred
about the cause of these bubbles?
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
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2. How did the mass of the egg change after it had been sitting in each of the different solutions for 24
hours? What caused the egg’s mass to change? (Include a separate two-part answer for each solution
type: vinegar, corn syrup, saltwater, and plain tap water).
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
3. Explain the changes in the egg’s mass in terms of osmosis.
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
4. Use a bar graph to show how each solution affected a change in the egg’s mass. (y-axis for mass, x-
axis for solution type, and two bars for each solution: one bar for the egg’s initial mass and the second
bar for its mass after 24 hours).
General Biology 1- Laboratory
Science, Technology, Engineering, and Mathematics
EXPERIMENT NO. 4
Egg’s Osmosis
DOCUMENTATION:
Direction: Include pictures of your group while doing the activity. Make sure that your faces are included. Put
a short label also for descriptions.
General Biology 1- Laboratory
Science, Technology, Engineering, and Mathematics
EXPERIMENT NO. 4
Egg’s Osmosis
SESSION REFLECTION:
Topic: ___________________________________________________
Direction: Please answer the question in this template based on your learning experience in this session.
1. What did you do in this session? (Give a summary of your learning activities).
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
2. What did you learn from this session?
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
General Biology 1- Laboratory
Science, Technology, Engineering, and Mathematics
EXPERIMENT NO. 4
Egg’s Osmosis
3. How will you apply what you learned?
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
4. What did you like the least in this session?
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
5. What did you like the most in this session?
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
6. What do you think can be done to improve this session or learning activities?
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
7. What have you discovered about yourself from this session’s learning experiences? What will you do
about it?
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
General Biology 1- Laboratory
Science, Technology, Engineering, and Mathematics
EXPERIMENT NO. 4
Egg’s Osmosis
General Biology 1- Laboratory
Science, Technology, Engineering, and Mathematics
