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Lab 2

This document provides instructions for using a microscope to view specimens. It describes how to set up the microscope by ensuring the light is on, placing a slide on the stage and securing it, centering the slide under the light, and using the coarse and fine focus knobs to bring the specimen into view. It instructs the user to first view the specimen under low power and then high power objectives, and notes that high power isn't always necessary. The document then has sections for observing and drawing a specimen, and answering questions about the relationship between the eyepiece view and stage view, how the microscope helped with drawing details, and the importance of microscopes for examining evidence.

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Joshua Reyes
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14 views2 pages

Lab 2

This document provides instructions for using a microscope to view specimens. It describes how to set up the microscope by ensuring the light is on, placing a slide on the stage and securing it, centering the slide under the light, and using the coarse and fine focus knobs to bring the specimen into view. It instructs the user to first view the specimen under low power and then high power objectives, and notes that high power isn't always necessary. The document then has sections for observing and drawing a specimen, and answering questions about the relationship between the eyepiece view and stage view, how the microscope helped with drawing details, and the importance of microscopes for examining evidence.

Uploaded by

Joshua Reyes
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Name: _________________________________________________________ Date: _____________________

LABORATORY NO. 2

THE MICROSCOPE
I – Objective:
 To familiarize yourself with how to use a microscope
 To practice focusing the microscope on your specimens
 To identify why high magnification isn’t always best
 To inspire interest in microscopy by viewing microscopic patterns in nature.
II – Materials:
Microscope, glass slide with cover, markers (red, blue, and black), plastic tape
III – Procedure:
Place an unknown substance on a glass slide and cover. Place the glass slide on the stage right under
the lower objectives and secure it using stage clip. Adjust the mirror to reflect light through the specimen.
Setting up the Microscope
To get your microscope ready for use, follow the procedure below:
1. Check for the source of light.
2. Lower your stage to its lowest possible setting. You can use your coarse focus knob for this. Usually, to
lower the stage you turn the knob toward yourself.
3. Place your first slide onto the stage. Make sure the slide is secured by the stage clips.
4. Center the slide. Make sure the specimen you want to be looking at is sitting right above the light
source. You should be able to see that the specimen is lit up by the sub-stage light.
5. Slowly raise the stage using the coarse focus knob until you achieve focus. As you raise the stage,
keep looking through the eyepiece. As the stage raises, you will slowly see the specimen come into
focus. Stop when the focus is achieved.
6. Rotate the objective turret to the 10x low power objective lens. This will increase the magnification
but will also likely lead to loss of focus. Use the fine focus knob (if your microscope has one) to adjust
the focus again until it is clear. Most specimens in this activity will only require the low power lens to
view them.

Part 1: Observe and Draw


(Observe and draw that you have observe.)

High Power Objective Low Power Objective


Part 2: Observation Report:

1. Describe the relationship between what you see through the eyepiece and what you see on the stage.
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2. How did the microscpe help you in drawing the details of the specimen you have observed?
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3. How important is the microscope in the process of examining the different evidences?

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