Veterinary Skills Checklist Submission
Veterinary Skills Checklist Submission
PURDUE UNIVERSITY
VETERINARY NURSING
August 2022
Enter dates and printed names for each task. You are responsible
for these, and to be sure the printed name is legible
This documentation is required by the AVMA for every graduate of the program, and is
required for completion of the final clinical rotation course.
2
Table of Contents
Restraint Page
Small Animal
Large Animal
Lab Animal
3
Administer Subcutaneous Injection to a Rabbit 22
Collect Blood from a Rabbit 22
Restrain Bird and Perform Physical Examination 23
Trim Nails on a Bird 23
Obtain a History 24
Recording Patient Treatment/Clinical Data on Hospitalized Patient 24
Participate in an Enema (Small or Large Animal) 25
4
Place a Stomach Tube in a Dog or Cat 38
Perform a Comprehensive Oral Health Assessment/Treatment in a Dog or Cat 39
Perform a Skin Scraping 40
Perform Visual Exam for External Parasites 40
Collect a Sample for Dermatophyte Culture & Inoculate Media 40
Perform a Schirmer Tear Test 41
Perform a Fluorescein Stain Test 41
Perform Tonometry 41
Application of Elizabethan Collar 42
Apply Emergency Bandage or Splint 42
Hospitalized Patient Care, Recordkeeping & Observation of a Critical Patient 43
Calculate, Monitor and Maintain Intravenous Fluids and IV Catheter Care 43
Closed-Chest Cardiopulmonary Resuscitation (CPR) – Small Animal 44
Anesthesia Tasks
Small Animal
5
Operating Room and Sterilization Tasks
Pharmacy Tasks
Necropsy Tasks
6
Chemistry Panel 75
Collect and Crossmatch Blood for Transfusion 75
Urinalysis 75
Serology 76
Imaging Tasks
Safety Procedures 82
Complete Radiology Log 82
Participate in Contrast Study 82
Lateral and Ventrodorsal Thoracic Radiograph 83
Lateral and Ventrodorsal Thoracic Radiograph 83
Lateral and Ventrodorsal Abdominal Radiograph 84
Lateral and Ventrodorsal Abdominal Radiograph 84
Ventrodorsal Extended Pelvis Radiograph (OFA View) 85
Create Diagnostic Intra-Oral Dental Radiographic Images 85
Lateromedial Projection of the Metacarpophalangeal (Fetlock) 86
Dorsopalmar Projection of the Metacarpophalangeal or Dorsoplantar Projection 86
of the Metatarsophalangeal (Fetlock)
7
Encage and Remove Dog from Cage
The student safely and appropriately approached the cage and opened the door
The student was able to remove the dog from the cage without injury to themselves or the patient
The student properly returned the dog to the cage without injury to themselves or the patient
The student safely and appropriately closed and secured the cage door
The student safely and appropriately approached the cage and opened the door
The student was able to remove the cat from the cage without injury to themselves or the patient
The student properly returned the cat to the cage without injury to themselves or the patient
The student safely and appropriately closed and secured the cage door
8
Canine Cephalic Venipuncture Restraint
9
Canine Eye / Ear Medication Restraint
The student was able to restrain the animal when it struggled such that the medication was
successfully administered and the applicator tip did not touch the animal
The student kept the nose tipped up and/or the head adequately controlled so that the medication
was successfully administered without contamination of the applicator tip
The student was able to restrain the animal in a manner that was adequate for control yet of no
harm to the animal
The student was able to restrain the animal when it struggled such that personnel were able to
carry out the IM injection or nail trim procedure without being bitten or injured
The student was able to restrain the animal in a manner that was adequate for control yet of no
harm to the animal
The gauze is of appropriate length to wrap around and tie top of the muzzle, tie beneath the dog’s
muzzle, and still have sufficient length to tie in a bow behind the head
The gauze muzzle was placed appropriately on the dog so that the mouth would not open
sufficiently to all biting
The muzzle was applied in such a way that the animal experienced minimal discomfort
10
Canine Nylon / Leather Muzzle Application
The student verified that the restraint pole is in good working order
The student properly placed the loop over the patent’s head and around the neck
The student closed the loop around the animal’s neck such that the animal could not escape, but
breathing was not restricted
The student safely removed the restraint pole from the animal
11
Feline Jugular Venipuncture Restraint
The student properly positioned the cat in sternal recumbency by holding the scruff of the neck and
pressing down on the cat with their other hand
The student was able to restrain the animal when it struggled
The student was able to control the head so as to prevent the other person or themselves from
being bitten or injured by movement of the head
The student was able to control the feet so as to prevent the other person or themselves from being
scratched or injured by the claws
The student was able to move so as to allow the other person to successfully perform a physical
examination, but was able to maintain control of the animal
The student was able to restrain the animal in a manner that was adequate for control yet of no
harm to the animal
The student properly positioned the cat in lateral recumbency with one hand on the scruff of the
neck and the other controlling the rear legs
The student was able to restrain the animal if it struggled
The student was able to control the head so as to prevent the other person or themselves from
being bitten or injured by movement of the head
The student was able to control the feet so as to prevent the other person or themselves from being
scratched or injured by the claws
The student was able to move so as to allow the other person to successfully perform the physical
examination, but was able to maintain control of the animal
The student was able to restrain the animal in a manner that was adequate for control yet of no
harm to the animal
12
Feline Towel Restraint
The student properly positioned the cat in sternal recumbency on the towel
The student positioned the cat so that it was perpendicular to the length of the towel with its head
off the edge of the towel
The student was able to wrap the cat in the towel to control the head so as to prevent other person
or themselves from being bitten or injured by movement of the head
The student was able to wrap the cat in the towel to control the feet so as to prevent the other
person or themselves from being scratched or injured by the claws
The student was able to restrain the animal in a manner that was adequate for control yet of no
harm to the animal
The student properly positioned the cat in sternal recumbency in the open bag
The student was able to close the bag with the cat inside the cat bag so as to prevent the other
person or themselves from being bitten or injured by movement of the head
The student was able to close the bag with the cat inside so as to prevent the other person or
themselves from being scratched or injured by the claws
The student was able to restrain the animal in a manner that was adequate for control yet of no
harm to the animal
13
Participate in Safe Operation of Cattle Chute
14
Haltering, Tying and Leading the Horse
The student positioned the unbuckled halter, in their left hand, for placement on the horse, with the
non-snap end of the lead rope in their right hand
Without quick movements and loud noises the student properly approached the patient at a 45°
angle to the patient’s left shoulder
The student placed the end of the lead over the horse’s neck, and passed sufficient length of lead
to form a handheld loop around the horse’s neck
Holding the handheld loop in their right hand, with their left hand, the student slipped the nose-band
of the halter over the nose
With their right hand under the horse’s neck, the student passed the crown strap over the head and
behind the ears and attached the end to the appropriate place on the halter
The student snapped the end of the lead to the lead ring of the halter and undraped the lead rope
from the horse’s neck
The student adjusted the halter so it was snug enough that the nose piece could not fall over the
end of the nose, but not so tight that the halter cut or rubbed the horse or restricted jaw movement
or breathing
The student did not restrain the horse by holding on the halter
The student folded the loose end of the lead in an accordion fashion, and held it in their left hand
The student positioned themselves on the left side of the horse, approximately midway along the
horse’s neck, 12 to 18” from the horse’s body, with their right hand grasping the lead approximately
6 to 12” from the lead ring of the halter
The student led the horse from the “neck” position
The student remained alert to the movements and reactions of the horse
The student did not lead from in front of the horse or lag back allowing the horse to pull them
The student appropriately tied the lead rope to an acceptable structure, then untied it
The student removed the halter from the horse and was observant when they walked away from the
horse
15
Attaching and Using a Chain Lead with the Halter Already in Placed on a Horse
The student properly approached the left side of the haltered patient
The student passed the snap end of the lead through the lower left ring of the halter, from outside to
inside
The student draped the chain over the nose of the horse, just rostral to the nose band of the halter
The student passed the snap of the lead through the left lower halter ring, from inside to outside
The student attached the snap with the button side out to the right ring of the halter
The button of the snap did not rub on the face of the horse
The chain moved freely through the lower halter rings, across the bridge and below the halter nose
band when drawn tightly or relaxed
The student removed the chain lead
The student properly approached the left side of a haltered horse, held on a lead by the assistant,
positioning themselves forward of the assistant and lateral to the head of the horse
With their left hand, the student passed their hand part way through the loop of the twitch, so that
their pinky or index finger and thumb were not through the loop and so that the loop would not slide
completely over their hand
The handle of the twitch was held in the student’s right hand and to the left of the horse
The student’s hand holding the loop was brought over the bridge of the nose and gently, but without
hesitation, brought down to the upper lip
The student grasped the upper lip and nose of the patient securely and slipped the loop of the
twitch off the fingers and over the upper lip and nose
The student twisted the handle of the twitch toward the head (clockwise) until the loop was snug, to
prevent the loop from falling off and to distract the horse, but not so much to cause excessive pain
The student instructed the assistant to hold the horse’s head to the left with the lead
The student did not pull the horses head to the left with the twitch
The student did not stand in front of the horse
The student applied pressure to the lip with a twist and not a pull
The student altered the pressure on the lip with a rocking motion, slightly releasing and re-tensing
the tension on the loop
The student kept both hands on the twitch during the procedure
The student released the twitch and rubbed the horse’s upper lip and nose
16
Snare Restraint of the Porcine Patient
Date completed _______________________________
The student isolated the rat without injury to either the animal or themselves
The student restrained the rat to limit head movement so that the student is not injured, by one of
the following methods:
1. Placing the thumb on one side of the thorax just caudal to the scapula and the remaining
fingers on the opposite side of the thorax caudal to the scapula
2. Placing the thumb and forefinger on each scapula and pushing toward the ventral midline,
making the forelimbs cross under the animal’s chin
3. Placing the index finger on one side of the head cranial to the forelimb. The thumb and ring
finger are just caudal to the scapula, wrapping around the thorax
The student provided support to the caudal half of the rat to control the animal and alleviate stress
while moving it to the examination table
The student moved the rat from its cage to the table without losing control of the animal
The student adequately controlled the rat when it struggled during the procedure
The student determined the sex of the rat
The student isolated the rat without injury to the animal or personnel
The student grasped the base of the tail
The student lifted the rat from the cage and immediately placed it on the exam table before the
animal showed signs of stress
The student moved the rat from its cage to the table without losing control of the animal
The student successfully controlled the rat when it struggled
19
Administer Intraperitoneal Injection to Rat
20
Restrain Rabbit in Towel with Head Exposed
The student scruffed the rabbit while the ears were pressed against the shoulders and placed a
hand under the rump for support
The student positioned the scruffed rabbit in dorsal recumbency so that the feet, legs and perianal
area could be examined
The student restrained the rabbit in a manner that was adequate for control yet of no harm to the
animal
The student immobilized the rabbit in a way that prevented personnel from being injured
The student was able to restrain the animal adequately when it struggled
The student determined the sex of the rabbit during restraint
21
Administer Subcutaneous Injection to a Rabbit
The student properly restrained the rabbit to immobilize and protect the animal from injuring itself
during the procedure
The student selected the proper site for blood collection
The student clipped the fur from the ear if necessary
The student placed the needle in the skin with the bevel facing up and in the proper location
The student’s hand was in the proper position to hold the syringe and aspirate to obtain the sample
The student placed a digit over the puncture site, removed the needle, and continued to apply
pressure to the site to prevent hematoma formation
22
Restrain a Bird and Perform Physical Exam
The student observed the bird to assess general appearance and condition before approaching it to
begin the physical exam
The student removed water bowls, perches and food containers from the carrier, if applicable
Room lights were dimmed to reduce stress on the bird, if applicable
The student approached the bird in a non-threatening manner
The student allowed the bird to see and interact with a towel before trying to place it over the bird
The student placed the towel over the bird’s head
The student appropriately grasped the bird firmly but gently around the neck while keeping the
towel over the bird’s head
The student removed the towel from over the bird’s head and wrapped the remaining portion of the
towel around the body of the bird to prevent wing flapping, and thus potential injury to the bird or
restrainer
The student effectively restrained the feet to prevent scratching of the restrainer
The student was careful not to compress the thorax of the bird during restraint, avoiding
unnecessary distress and inadvertent suffocation of the bird
The student examined the bird’s face, beak, and inside the mouth
The student ausculted the heart and air sacs
The student examined the cloaca for discharge
The student accurately recorded findings
The student submitted a written physical exam report
23
Obtain a History – Small or Large Animal
The student allowed the client to state the presenting problem before asking additional directed
questions
The student asked the questions clearly and used terminology the client understood so that the
client was able to answer the question accurately
The student maintained good communication skills:
-good eye contact
-non-verbal body language that encouraged the client to continue to speak
-allowed the client to finish a statement without interrupting
The student asked questions in such a way that the question was not a leading
question (e.g. “you did see diarrhea, didn’t you?”)
When/if a client was unable to understand a question the student was able to
formulate a different way of asking the same question and obtaining the needed
information
The student periodically repeated the information back to the client to confirm what
the student heard was a correct interpretation of what the client said or meant
The student was able to direct the history taking dialogue to obtain the information in
a timely manner (i.e., didn’t allow the conversation to wander too far from the goal of
getting a complete and accurate history
The student was able to establish a working rapport with the client. The student
conducted the history interview in a courteous and professional manner
The student was able to gauge the amount of history needed based upon the critical
status of the patient (i.e., if the case was an animal in critical status, only the
pertinent history was obtained before emergency treatment was begun)
The student accurately recorded the history obtained from the client in sufficient
detail to convey all the information needed by the veterinarian
The student accurately identified the patient on the Hospitalization Record form
The student accurately recorded the clinical status of the animal
The student obtained and recorded an accurate TPR
The student accurately recorded any treatments performed on the animal
The student accurately recorded any medications administered to the animal
The student accurately recorded the veterinarian’s orders for this patient
The student accurately recorded any additional clinical data as directed by the veterinarian
24
Participate in an Enema (Small or Large Animal)
The student observed the patient to assess attitude before approaching the animal to begin
the physical examination
The student examined each of the following items on the physical examination
Temperature, pulse, respiration
Capillary refill time
General appearance
Integumentary system
Musculoskeletal system
Circulatory system
Respiratory system
Genitourinary system
Nervous system
Ears
Eyes
Lymph nodes
Mucous membranes
The student accurately recorded the findings of the physical examination including any
abnormalities
25
Perform a Physical Examination on a Feline Patient
The student observed the patient to assess attitude before approaching the animal to begin
the physical examination
The student examined each of the following items on the physical examination
Temperature, pulse, respiration
Capillary refill time
General appearance
Integumentary system
Musculoskeletal system
Circulatory system
Respiratory system
Genitourinary system
Nervous system
Ears
Eyes
Lymph nodes
Mucous membranes
The student accurately recorded the findings of the physical examination including any
abnormalities
26
Administer Oral Tablet or Capsule to a Cat
The student maintained control of the head or muzzle during the administration of the
medication
The medication was swallowed without choking
The student was able to control the animal in a manner that was adequate to administer the
medication yet did no harm to the animal
27
Administer Ear Medication to a Dog
The student was able to place the medication applicator in the proper position
The student maintained control of the head or muzzle during the administration of the
medication
The student successfully administered the medication into the ear canal
The medication was administered without contamination of the applicator tip
The student was able to control the dog in a manner that was adequate to administer the
medication yet did no harm to the animal
The student was able to place the medication applicator in the proper position
The student maintained control of the head or muzzle during the administration of the
medication
The student successfully administered the medication into the ear canal
The medication was administered without contamination of the applicator tip
The student was able to control the cat in a manner that was adequate to administer the
medication yet did no harm to the animal
28
Administer Subcutaneous Injection to a Cat
The student selected the proper site for administration based on anatomical landmarks
The student properly introduced the needle into the site of administration
The student aspirated the syringe to check for blood prior to injection
The student successfully administered the prescribed amount of medication
The student selected the proper site for administration based on anatomical landmarks
The student properly introduced the needle into the site of administration
The student aspirated the syringe to check for blood prior to injection
The student successfully administered the prescribed amount of medication
29
Perform Cephalic Venipuncture in a Dog
30
Perform Femoral/Medial Saphenous Venipuncture to a Cat
31
Administer Intravenous Injection to a Cat
The student placed the nail trimmers in the proper position on the nail
The amount of nail tip removed was appropriate
The nail did not bleed after removal of the tip
The student placed the nail trimmers in the proper position on the nail
The amount of nail tip removed was appropriate
The nail did not bleed after removal of the tip
32
Ear Sample Collection and Preparation in a Dog or Cat
The student introduced the cotton-tipped applicator appropriately into the ear
The swab, containing an appropriate sample, was removed from the ear and the sample
applied to either a culture medium, transport medium (for transport to an outside laboratory for
culture), or onto a slide (for examination for yeasts, parasites, and bacteria)
The student stained the slide appropriately for yeast or bacteria
The student applied mineral oil to the slide to look for otic parasites
The student appropriately administered cleaning solution to the ear canal in sufficient quantity
The solution was administered without contamination of the applicator tip
The student massaged the ear canal externally
The student cleaned the outer ear with cotton balls
The student avoided placing swabs into the ear canal
The ear canal was clean after the cleaning process
The student appropriately administered cleaning solution to the ear canal in sufficient quantity
The solution was administered without contamination of the applicator tip
The student massaged the ear canal externally
The student cleaned the outer ear with cotton balls
The student avoided placing swabs into the ear canal
The ear canal was clean after the cleaning process
33
Express Anal Sacs in a Dog
Anal sacs were expressed and toe nail trims were done if required
The student removed any collars or harnesses, cotton was placed in the ears
The student thoroughly wetted the animal prior to application of the shampoo
The student appropriately applied the shampoo and observed precautions
The hair and skin were thoroughly rinsed of all shampoo
Cotton balls were removed from the ears
The animal was dried safely, adequately, and not overheated with a cage dryer
Animal was combed or brushed, and mats removed if required
The student selected a clean container that was appropriate for the amount
The student collected the urine after the initial stream
The student avoided contaminating the collected urine by contact with the animal’s hair, etc
The student collected at least 8 cc’s of urine from a dog (or 3 cc’s from a cat)
The student correctly placed his/her finger in the jugular groove to act as a tourniquet
The student palpated the area to determine the location of the jugular vein
The student placed the needle in the skin with the bevel up and in the proper location
The student student’s hand was in the proper position to hold the syringe and draw back on
the plunger to obtain the sample
The student acquired the necessary volume for the tests ordered
The student released the digital tourniquet when the appropriate volume was achieved
The student placed a digit over the puncture site
The needle and syringe were removed from the patient and digital pressure was continued to
prevent a hematoma
Supervisor Name ______________________________________________ RVT/DVM
The student correctly placed his/her finger in the jugular groove to act as a tourniquet
The student palpated the area to determine the location of the jugular vein
The student placed the needle in the skin with the bevel up and in the proper location
The student student’s hand was in the proper position to hold the syringe and draw back on
the plunger to obtain the sample
The student acquired the necessary volume for the tests ordered
The student released the digital tourniquet when the appropriate volume was achieved
The student placed a digit over the puncture site
The needle and syringe were removed from the patient and digital pressure was continued to
prevent a hematoma
The student clipped an area of appropriate size, leaving no hair at the site
The student prepped the site for aseptic catheter placement and did not contaminate the site
The student flushed the catheter with heparinized saline prior to placement
The student placed the catheter into the skin with the bevel up
The student looked for blood flow into the catheter and when blood was observed, threaded
the catheter off the stylet into the vein
The student secured the catheter to the patient’s leg with tape without kinking the catheter
The student flushed the catheter with heparinized saline
The student clipped an area of appropriate size, leaving no hair at the site
The student prepped the site for aseptic catheter placement and did not contaminate the site
The student flushed the catheter with heparinized saline prior to placement
The student placed the catheter into the skin with the bevel up
The student looked for blood flow into the catheter and when blood was observed threaded
the catheter off the stylet into the vein
The student removed the stylet from the catheter and placed an injection cap on the catheter
The student secured the catheter to the patient’s leg with tape without kinking the catheter
The student flushed the catheter with heparinized saline
The student clipped an area of appropriate size leaving no hair at the site
The student prepped the site for aseptic catheter placement and did not contaminate the site
The student flushed the catheter with heparinized saline prior to placement
The student placed the catheter into the skin with the bevel up
The student looked for blood flow into the catheter and when blood flow as observed,
threaded the catheter off the stylet into the vein
The student removed the stylet from the catheter and placed an injection cap on the catheter
The student secured the catheter to the patient’s leg with tape without kinking the catheter
The student flushed the catheter with heparinized saline
The student applied tape stirrups to the distal portion of the limb
The student applied two layers of soft padding onto the limb starting at the distal portion of the
limb. The student made sure it was not too tight before starting the next layer
The student applied a layer of gauze to the limb, starting at the distal portion of the limb. The
student made sure it was not too tight before starting the next layer
The student reflected the tape stirrups to adhere to the gauze
The student applied a layer of protective cover to the limb, making sure it was not too tight
The limb was in proper position once the bandage was complete. The student checked the
toes for temperature and swelling
The student appropriately removed the bandage from the patient
36
Administer Subcutaneous Fluids to a Dog or Cat
The student chose the proper needle size for the patient
The student placed the needle in the correct area
The student administered the appropriate amount of fluids at each site
The student palpated the bladder while the patient was restrained in lateral, dorsal or standing
recumbency
The student chose the appropriate size needle for the patient and the appropriate size syringe
for the sample to be obtained
The student examined the area of the needle placement to ensure its cleanliness and
prepared it with alcohol
The student placed the needle on the midline (female)
The student placed the needle in the correct area and avoided the prepuce (male)
The student aspirated to determine if the needle was in the bladder. If the needle was in the
bladder, the student drew enough urine for the sample
If the needle was not in the bladder, the student released negative pressure on the syringe,
withdrew the syringe and needle and got a new syringe and needle to try again. The student
did not redirect within the abdomen
The student did not aspirate while placing or withdrawing the needle
The student chose the proper type and size catheter for the dog
The student, while an assistant exposed the penis, prepared the penis aseptically without
touching the prepuce
The student lubricated the distal end of the catheter without contaminating the catheter
The student introduced and passed the catheter into the bladder without contamination
The student emptied the bladder with a syringe if the catheter was to removed
The student attached a collection system to the catheter if the catheter was to stay in
The student had an assistant hold the dog in either sterna or standing recumbency
The dog was held firmly to minimize movement prior to sampling
The student properly placed a lubricated speculum into the vagina
The student moistened a sterile cotton swab with sterile saline
The student collected the sample as far cranial as possible without contaminating the
sample/swab
The student rolled the swab across the slide to distribute the cells along the slide
The student stained the slide with Diff-Quick
The student measured the tube, placing the tip of the tube at the last rib and marking the tube
at the point of the mouth prior to placement
The student placed the mouth speculum in the mouth in the correct position
The student properly lubricated the stomach tube
The student properly passed the tube into the dog’s stomach without force
The student verified the tube was in the stomach by palpating the tube within the neck,
injecting 100 mL of air and an assistant auscultating the stomach for gurgling, or injecting 50
mL of sterile saline and listening for a cough
The student administered the medication into the stomach tube (Tap water may be used if
needed)
The student sealed the end of the tube to prevent aspiration of medication and removed the
stomach tube from the dog
38
Perform a Comprehensive Oral Health Assessment and Treatment in a Dog or Cat
The student performed an oral exam to evaluate occlusion, any retained deciduous or
deformed teeth, and evaluate the amount of calculus present
The student examined the gingival tissues and pocket depths around all of the teeth with a
periodontal probe and noted the abnormalities on either the dental or patient chart
The student placed towels under the patient’s head and neck to prevent potential aspiration of
water and debris
The student wore a mask, exam gloves and goggles before beginning the scaling of the teeth
The student properly hand scaled the subgingival area of the teeth with a manual instrument
The student removed the calculus from the supragingival area of the teeth, by either starting
out with manual instruments and then using the ultrasonic scaler, or just using the ultrasonic
scaler
When manual instruments were used, the student maintained the proper angle with the
scaling instrument and moved from the gingival edge toward the crown
The student did not leave the ultrasonic scaler on the tooth for longer than 10-15 seconds at a
time
The student maintained the side of the ultrasonic scaler parallel to the tooth and worked with
overlapping strokes from the gingival edge toward the crown
The student polished the teeth using the polish device at low speed, moving from each tooth
every 2-3 seconds
The student rinsed the teeth with water to remove any residual debris from the mouth
The student performed a post cleaning oral exam and charted the teeth on the appropriate
dental chart or record
The student prepared a set of home care instructions for the client
39
Perform Skin Scraping
The student placed a drop of mineral oil on the microscope slides being used
The student moistened the scalpel blade with mineral oil
The student selected an appropriate site/lesion for scraping
The student pinched a fold of skin and scraped the surface until drops of capillary blood
appeared
The student transferred the material collected onto the glass slide with mineral oil
The student thoroughly inspected the animal from head to tail, looking for the presence of
external parasites
The student parted the hair on the animal in several places in order to directly observe the
skin the evidence of external parasites or flea allergy dermatitis
The student examined the ears for evidence of external parasites
The student properly used a flea comb on the hair of the animal
The student placed the material collected with the flea comb onto a moist white paper to
examine it for evidence of flea “dirt”
40
Perform Schirmer Tear Test
The student prepared the test strip, folding at the notch while still in the package
The student removed the strip from the package, touching only the end that is not placed on
the eye
The student inserted the strip between the lower eyelid and the cornea
The student held the strip in place for 60 seconds, preventing the animal from rubbing the eye
or removing the strip
The student removed the strip from the eye and measured the length of the strip that was we
according to the manufacturer’s instructions
The student moistened the end of a sterile Fluorescein stain strip using sterile eye wash or
artificial tear solution
The student elevated the upper eyelid
The student placed the moistened tip of the strip on the bulbar conjunctiva for 1-2 seconds or
further moistened the strip and allowed the stain to drip onto the cornea
The student removed the strip (if touched to the eye) and allowed the animal to blink
The student flushed the eye with sterile eyewash
The student examined the cornea in a partially darkened room
Perform Tonometry
The student checked the tonometer for function and cleanliness before using (calibrated)
The student instilled topical ophthalmic anesthetic drops in both eyes without touching the tip
of the bottle to the eye
The student waited 30-60 seconds before beginning the test
The student assured the animal’s head was restrained and positioned for the procedure
The student placed the tonometer on the animal’s cornea and noted the reading
If a Schiotz was used, the student converted the values to mg Hg
41
Application of Elizabethan Collar
The student chose the correct size Elizabethan collar for the patient
The student prepared/assembled the collar as needed
The student applied the collar to the patient without causing injury or discomfort
The student secured the collar so it could not be removed by the patient, but was not too tight
Once placed, the collar prevented the patient from either chewing or scratching
inappropriately
The student applied tape stirrups to the distal portion of the limb
The student covered any open wounds with non-adhering gauze pads and secured them with
tape
The student applied either roll cotton (Robert Jones bandage) or cast padding (splint) to the
limb, starting at the distal portion of the limb
The student appropriately applied a layer of gauze to the limb, starting at the distal portion of
the limb
If using a splint, the student chose an appropriate splint and applied it to the limb
The student reflected the tape stirrups proximally on the limb
The student applied a layer of protective tape such as Vetwrap® or Elastikon® to the limb
The student checked the bandage after each layer to make sure it was not too tight
The limb was in proper position once the bandage was complete
The student checked the toes for temperature or swelling
42
Hospitalized Patient Care, Recordkeeping and Observation of a Critical Patient
The student chose and identified the patient by its signalment and ailment and recorded the
information on the patient record
The student chose a critical case such as an unregulated diabetic, HBC, renal failure,
pancreatitis, etc.
The student provided care for at least 4 hours of the patient’s hospitalization
The student initialed each entry to verify they performed the observation and treatment during
the 4 hours
The student recorded the hourly monitoring and observation parameters accurately and
chronologically
The student recorded all treatments administered during the monitoring period accurately
The student brought variations from normal parameters to the attention of the veterinarian in
charge of the patient
The record was clear, accurate and easy to follow
Calculate, Monitor and Maintain Intravenous Fluids and Provide IV Catheter Care
The student chose a case which required intravenous fluids for a minimum of 12 hours
The student showed their calculation of the flow rate for the IV fluids for the patient
The student set the flow rate to deliver the desired amount
The student recorded the volume of fluid administered hourly, as well as the total for the day
The student maintained the correct rate of fluid administration
The student monitored the IV catheter site for signs of swelling, redness or non-patency
The student specifically observed the patient for parameters related to hydration status,
including respiration rate and character, lungs sounds, skin turgor, mucous membrane color
and character, and capillary refill time
The student monitored the patient and recorded all data for at least 4 hours
43
Closed-Chest Cardiopulmonary Resuscitation (CPR) – Small Animal
The student simulated checking the patient for heartbeat and respiration
The student simulated placing an appropriate size endotracheal tube, using a laryngoscope
and securing it with gauze
The student simulated administration of oxygen using the proper delivery system and oxygen
flow rate
The student simulated ventilation of the animal at the proper rate and pressure
The student simulated performing chest compressions, using the proper technique for the
patient, at the proper rate
The student demonstrated the technique for abdominal compressions/counterpulsions with an
assistant performing chest compressions
The student simulated intravenous catheter placement and administration of fluids
The student simulated the drawing and administration of emergency drugs as directed by the
veterinarian
The student attached an ECG monitor to the patient
The student observed the patient to assess attitude before approaching the patient to begin
the physical examination
The student counted the respiration rate of the patient by observing the nostril
movement/flares or chest movement for 30 seconds and recorded the number of respirations
in breaths per minute
The student located and palpated an artery or ausculted the chest of the patient, counted for
15 seconds and recorded the heart rate results in beats per minute
The student examined each of the following items on the physical examination:
CRT/MM Circulatory system
General appearance Respiratory system
Body condition scoring Genitourinary system
Integumentary system Nervous system
Musculoskeletal system Ear
The student accurately recorded the findings of the physical examination including any
abnormalities
44
Perform a Physical Examination in an Equine Patient
The student observed the patient to assess attitude before approaching the patient to begin
the physical examination
The student counted the respiration rate of the patient by observing the nostril
movement/flares or chest movement for 30 seconds and recorded the number of respirations
in breaths per minute
The student located and palpated an artery or ausculted the chest of the patient, counted for
15 seconds and recorded the heart rate results in beats per minute
The student examined each of the following items on the physical examination:
CRT/MM Circulatory system
General appearance Respiratory system
Body condition scoring Genitourinary system
Integumentary system Nervous system
Musculoskeletal system Ear
The student accurately recorded the findings of the physical examination including any
abnormalities
The student correctly placed his/her finger in the jugular groove to act as a tourniquet
The student palpated the area to determine the location of the jugular vein
The student wiped the area with alcohol, then placed the needle in the skin with the bevel up
and in the proper location, then attached the syringe
The student’s hand was in the proper position to hold the syringe and draw back on the
plunger to obtain the sample
The student released the digital tourniquet when the appropriate volume was achieved
The student placed a digit over the puncture site
The needle was removed from the patient; digital pressure was applied to prevent hematoma
45
Administration of an Intravenous Injection in a Bovine Patient
46
Administration of Bolus Medication in a Bovine Patient
The student restrained the head of the patient and was able to open the mouth of the patient
The student maintained control of the head during the administration of the medication
The student used the appropriate size balling gun for the patient
The student inserted the balling gun in the patient’s mouth and over the tongue
The medication was swallowed without choking
The student was able to control the patient in a manner that was adequate to administer the
medication yet did not harm the patient
Supervisor Name ______________________________________________ RVT/DVM
The student correctly placed his/her finger in the jugular groove to act as a tourniquet
The student palpated the area to determine the location of the jugular vein
The student wiped the area with alcohol, then placed the needle in the skin with the bevel up
and in the proper location, then attached the syringe
The student’s hand was in the proper position to hold the syringe and draw back on the
plunger to obtain the sample
The student released the digital tourniquet when the appropriate volume was achieved
The student placed a digit over the puncture site
The needle was removed from the patient; digital pressure was applied to prevent hematoma
The student correctly placed his/her finger in the jugular groove to act as a tourniquet
The student palpated the area to determine the location of the jugular vein
The student wiped the area with alcohol, then placed the needle in the skin with the bevel up
and in the proper location, then attached the syringe
The student’s hand was in the proper position to hold the syringe and draw back on the
plunger to check for blood prior to injection
The student injected the drug without signs of extravasation
The student placed a digit over the puncture site
The needle was removed from the patient; digital pressure was applied to prevent hematoma
47
Administration of Intramuscular Injection in an Equine Patient
In an adult horse, the student prepared the serratus cervicis for the IM injection, and
pointed out and listed the anatomical borders of the site
Without the needle attached to the syringe, while holding the hub of the needle between their
index and middle figure and the shaft of the needle toward their palm, the student tapped the
side with the back of their hand or fist and without hesitation, turned the needle toward the
neck and inserted it through the skin and deep into the muscle
The student attached the syringe to the needle, aspirated, and injected 1 cc of saline
There was no blood return on aspiration
The student rubbed the site after withdrawal of the needle
The student collected milk samples from each quarter in four clean CMT paddle cups labeled
A,B,C,D
The student added an equal volume of CMT solution to each cup
The student rotated the CMT paddle in a circular motion for 10 seconds to thoroughly mix the
contents
The student read the test immediately and classified results based on the presence or lack of
thickening/gelling due to the presence/absence of DNA from white blood cells in the sample
48
Lower Leg Wrap in the Horse
The student properly approached the patient on the left side, properly moved to the patient’s
hind quarters, and stood to the side for the procedure
The student removed debris or chaff from the tail
The student unrolled approximately 12 inches of wrap, lifted the tail slightly, slid a single layer
under the tail, and moved it up toward the tail head as high as possible without wrinkling or
folding the wrap or rumpling the hairs. The wrap was positioned so it unrolled from the top of
the leading edge
The student brought the leading edge of the wrap over the top of the tail and began to unroll
the wrap over the leading edge
After two wraps at the top, the student continued distally, maintaining ever tension and
overlapping the wrap approximately half of the previous wrap
Every 3-4 wraps, the student folded a tuft of hair into the wrap
The student continued wrapping to a point 2-3 inches below the caudal vertebrae and secured
the wrap with the device appropriate for the wrap utilized
49
Placement of Jugular Intravenous Catheter in a Large Animal
The student clipped an area of appropriate size, leaving no hair at the site
The student prepped the site for an aseptic catheter placement and did not
contaminate the site once it was prepped
The student “tented” the skin and inserted the needle with the bevel up
The student directed the needle into the vein and inserted the needle
The student observed the catheter for blood flow and when blood was seen,
advanced the length of the catheter into the vein
The student placed the needle guard over the needle
The student removed any protective sleeve or stylet associated with the catheter
The student placed an injection cap or extension set with cap on the catheter
The student flushed the catheter with heparinized saline, aspirated blood to check
proper placement and patency, and flushed the catheter again
The student secured the catheter with appropriate bandage material and technique
50
Anesthetic Delivery and Monitoring Equipment Use and Maintenance
The student checked the oxygen source to verify there was enough oxygen to complete the
procedure if applicable
The student turned the oxygen on and verified the oxygen pressure gauge was working and
the flow meter was functioning by turning on the flow meter temporarily to watch the ball move
to the desired oxygen flow
The student checked the soda lime canister to ensure the granules were fresh according to
the practice standard operating procedure
The student checked the vaporizer to make sure there was inhalant agent in the vaporizer and
that it was at least half full and the dial moved smoothly
The student attached the proper breathing system and breathing bag for the patient being
anesthetized
The student raced the flow from the source, to the patient, from the patient and back to the
scavenge system to ensure all connections were correctly assembled
The student performed a low-pressure leak test to ensure all connections were secure and no
leaking of gas would occur
The student opened the pop-off valve to ensure it was not stuck or closed prior to anesthesia
51
Preparation of Supplies Prior to General Inhalant Anesthesia – Small Animal
The student chose at least 2 endotracheal tubes of appropriate size for the patient to be
anesthetized, checked the cuffs for leaks, and placed them at the induction area
If the case is a feline, the student prepared lidocaine spray/gel and a stylet to aid in intubation
The student placed a piece of non-stretch gauze near the endotracheal tubes for use in tying
the tube. The piece was of adequate length to tie around the tube
The student placed a syringe near the endotracheal tubes for use in filling the cuff after
intubation
The student placed a syringe of heparinized saline at or near the induction site to use in
verifying intravenous catheter patency
The student prepared an intravenous catheter of appropriate size for the patient being
anesthetized
While leaving the catheter in the package, the student opened the catheter, removed the cap,
flushed the catheter with heparinized saline and placed at or near the induction area
The student placed clippers and aseptic preparation materials at or near the induction are for
clipping and prepping the intravenous catheter site
The student provided ophthalmic lubricating ointment at or near the induction are to lubricate
the eye after induction
The student calculated oxygen flow rate prior to induction
The student provided an oxygen mask near the induction area to provide oxygen or inhalant
agent prior to intubation if needed
The student had a stethoscope and other monitoring devices (depending on practice standard
operating procedure) ready for use at the induction area and verified they were in working
order
The student located and made others aware of the location of the emergency supplies in case
they are needed during the procedure
The student provided towels, blankets and other methods for keeping the patient warm at the
area
The student set up intravenous fluids with an administration set at the induction area for use
during anesthesia
The student prepared the anesthesia record and placed it at the area for induction
The student prepared the anesthesia induction agent so it was ready to administer at time of
induction
52
Pre-Anesthetic Preparation of the Patient for Anesthesia – Small Animal
The student identified the patient’s chart and reviewed it to ensure that the appropriate
laboratory tests had been performed as defined by the practice standard operating procedure
The veterinarian was consulted regarding results prior to premedication or induction of general
anesthesia
The student performed a physical exam prior to administering any anesthetic premedication
any abnormalities were brought to the attention of the veterinarian
The student reviewed the procedure to be performed and the patient’s condition, and
prepared according to the facility SOP for premedication, general anesthesia and
maintenance
The student calculated the dosages of preanesthetic agents and induction agents as
prescribed by the veterinarian, and had those doses checked by the mentor prior to
administration
The student administered the approved premedications to the patient at least 15-30 minutes
prior to induction of general anesthesia
The student checked the syringe to verify the amount drawn up in the syringe matched the
calculations
The student removed any air bubbles in the syringe
The student flushed the patient’s catheter with heparinized saline to ensure its patency
The student checked the work area one last time to make sure all materials were ready
The student, with an assistant holding the patient, administered the induction agent according
to practice standard protocol
The student attempted to open the patient’s mouth to determine if more induction agent was
needed to intubate
53
Intubation of a Dog
The student waited until the assistant opened the mouth and the dog did not resist opening of
the mouth
The student chose an appropriate endotracheal tube, and used the tube to push the tongue
out to the side of the mouth so the assistant could grasp it with a gauze sponge and extend
the tongue over the lower canine teeth. The assistant or student did NOT place their hands in
the dog’s mouth at any time
The student attached the breathing tubes, palpated at the thoracic inlet to feel the tip of the
endotracheal tube and to verify that only one tubular structure existed
The student used roll gauze to secure the endotracheal tube
The student inflated the cuff until no leak was heard when inflating the lungs to a pressure of
20 cm H2O a small leak should be heard when inflating the lungs past 20 cm H2O
Intubation of a Cat
The student waited until the assistant opened the mouth and the cat did not resist opening of
the mouth to apply lidocaine to the larynx
The student chose an endotracheal tube, placed a stylet in the tube and used the tube to push
the tongue out to the side of the mouth so the assistant could grasp it with a gauze sponge
and extend the tongue over the lower canine teeth. The assistant or student did NOT place
their hands in the mouth at any time
The student visualized the opening of the trachea, placed the tube in the trachea without
force, and pulled the stylet from the tube
The assistant held the cat’s mouth shut and the cat was laid on its side while the student
attached the breathing tubes, palpated at the thoracic inlet to feel the tip of the endotracheal
tube and to verify that only one tubular structure existed
The student used roll gauze to tie the endotracheal tube behind the cat’s head, and behind
both ears
The student inflated the cuff until no leak was heard when inflating the lungs to a pressure of
20 cm H2O a small leak should be heard when inflating the lungs past 20 cm H2O
54
Maintenance and Monitoring of General Anesthesia – Small Animal
The student set the oxygen flow rate according to the patient’s weight and requirement based
on the breathing system
The student adjusted the vaporizer setting to 15-3% based on the patient’s response to the
induction agent
The student verified that the patient was breathing and recorded a heart rate before
proceeding further to ensure the patient was stable following induction and intubation
The student placed an esophageal stethoscope into the esophagus (if monitoring) order to
facilitate quick removal if an emergency arose
The student attached the ECG (or appropriate alternative) according to the practice standard
operating procedure
The student attached intravenous fluids to the catheter and set the rate for surgical
maintenance as ordered by the veterinarian
The student manually squeezed the rebreathing bag every 1-2 minutes regardless of the
patient’s respiratory rate to 15-20 cm H2O
The student recorded values including heart rate, respiratory rate, anesthetic gas
concentration and fluid volume administered (as well as any other parameters being
monitored) on the anesthesia record every 5 minutes (every 30 minutes for fluids)
The student brought abnormal readings to the attention of the veterinarian
The student checked the patient’s reflexes (palpebral, pedal, jaw tone, eye position,
depending on accessibility) to ensure the patient was neither too deep nor too light, and
brought abnormal responses to the attention of the veterinarian
The student observed the patient’s respiratory function by observing the rebreathing bag to
count rate and observing chest excursions to ensure adequate depth of each breath
The student maintained the anesthetic gas concentration at the lowest level possible to
achieve general anesthesia
The student decreased the anesthetic concentration near the end of the procedure
55
Recovery from General Anesthesia – Small Animal
The student turned off the inhalant anesthetic gas and administered oxygen for a period of 2-5
minutes to scavenge waste gases prior to disconnecting the breathing circuit
The student inspected the oral cavity to insure it was free of secretions and/or objects that
could impede respiration
The student maintained the patient’s head in a normal position
The student deflated the endotracheal tube cuff and untied it from the patient to facilitate quick
removal
If placed, the student removed the esophageal stethoscope and other monitoring devices prior
to the patient awakening from general anesthesia
The student removed the endotracheal tube when the patient began to swallow (2-3 time)
without stimulation
The student observed the patient following extubation for signs of respiratory distress and/or
cyanosis, and informed the veterinarian if abnormalities were noted
If abnormalities were noted, the student administered oxygen while awaiting the arrival of the
veterinarian
The student recorded heart rate, respiratory rate and temperature following extubation. Values
were recorded every 5 minutes for the first 15 minutes following extubation. Abnormalities
were brought to the attention of the veterinarian
The student used available means to elevate body temperature to normal. The student
recorded the patient’s temperature every 30 minutes to insure the patient did not become
overheated. Heating methods were discontinued once the patient’s temperature reached 100
degrees Fahrenheit
The student placed the patient (if recumbent) in the opposite recumbency as it was during the
procedure to assist in ventilating the previously “down” lung field
If indicated, IV fluids were continued and the rate and catheter site monitored
The patient was monitored for signs of pain and analgesics administered as needed on the
orders of a DVM, and recorded in the patient record
The patient was monitored closely for respiratory depression if narcotic analgesic were
administered
The student recorded recovery parameters and notes at the bottom of the anesthetic record to
become a part of patient’s permanent record
56
Provide Post-Surgical Clean Up and Operating Room Sanitation and Care
The student gathered the appropriate instruments, and instrument plan if applicable
The student gathered the appropriate linen supplies if applicable
The student selected the appropriate packaging material and chemical indicator
The student assembled the pack correctly by following the instructions on the checklist or
recipe
The student appropriately selected and placed the chemical indicator
The student appropriately selected and utilized packaging material
57
Sterilize an Instrument Pack
Date completed________________________________
The student selected appropriately prepared instrument or item, in a peel pouch or wrapped
Note: A package of 10-20 gauze squares would be considered an individual item
The student placed the packaged item appropriately into the autoclave
The student identified the type of steam autoclave: hi-vac or gravity
The student identified the steam autoclave settings for processing the item, including
temperature (degrees F or C), exposure time (minutes), and dry cycle time (minutes)
The student operated the steam autoclave properly
The student opened the autoclave door slightly for at least 10 minutes but not more than 20
minutes following processing was complete
The student removed the item from the autoclave with attention to personal safety and without
compromising sterility (i.e., used oven mitt)
The student placed the cooled item on a clean, flat surface
If item wrapped: The student opened the item to show that chemical indicators had changed
to indicate successful sterilization, stating verbally the changes noted
If item in peel pouch: The student inspected the package to ensure it was intact, and stated
verbally the result of the inspection as well as the chemical indicator reading
58
Open a Surgical Pack
59
Participate in Ovariohysterectomy in a Dog
60
Clip and Prepare a Surgical Site
The student defined the area to be clipped using the correct anatomical landmarks
The student clipped all the hair from the surgical site according to the defined landmarks
The student applied antiseptic scrub to the site
The student prepped the site with a clean surgical sponge beginning at the incision site and
worked toward the edges
The student discarded the sponge once it reached the edge of the clipped area
The student did not bring the sponge back to the incision site once it was moved away from
the incision site
The student wiped the site with a rinse solution using a clean surgical sponge following the
same pattern as when scrubbing with the antiseptic
The student repeated the scrub and rinse a minimum of 3 times or until the final rinse sponge
was clean
The student identified the brush as a disposable brush or a clean re-sterilized brush
The student applied the antiseptic scrub over both arms and hands
The student began scrubbing the first hand and arm beginning at the fingertips
The student scrubbed ends of the fingers 10 times
The student scrubbed each of the four surfaces of each finger 10 times
The student scrubbed each of the four sides of the hand 10 times
The student scrubbed each of the four sides of the arm 10 times
The student repeated the same process on the second hand and arm
The student repeated the procedure on both hands and arms one more time
The whole procedure lasted a minimum of 5-7 minutes
The student did not touch any non-sterile objects
The student kept their hands above the elbows at all times during and after the scrub
61
Aseptically Don Surgical Gown
The student picked up the towel from the opened sterile gown without contamination or
dropping the hands below the elbows
The student dried the first hand beginning at the fingers and working toward the elbow
The student discarded the towel without contamination
The student removed the sterile gown from the wrap and shook it out without contamination
The student placed both arms in the sleeves and worked the gown on without contamination
The hands of the student did not exit the sleeves of the gown
The student stood with arms at chest while the shoulders and back of gown are tied by
another person
The student picked up the first glove from the open package and placed it thumb toward the
palm and fingers pointing towards the elbows
The student grasped the glove on both sides through the sleeves of the gown and stretched
the glove to create an opening
The student pulled the glove over the hand
The student moved their fingers from inside the cuff to the glove
The student placed their fingers into the fingers of the glove
The student pulled the sleeve of the gown and adjusted the glove to fit
The student repeated the process with the other glove and hand
The student made final adjustments on the gloves so that they fit snugly on the hands
The student did not contaminate the gloves or gown while putting the gloves on their hands
62
Aseptically Pass Instruments as Scrub Nurse
The student passed the blade with the left hand to a right handed surgeon
The sharp edge of the blade was pointed away from the student’s hand
The blade was visible at all times by the student and the surgeon
The handle was placed firmly into the surgeon’s hand
The surgeon was able to use the instrument with minimal adjustment
When the instrument was returned to the student, it was wiped clean of blood and replaced on
the organized table
Towel clamp
The student held the towel clamp so that when it was passed to the surgeon, it was used with
minimal adjustment
The student “snapped” the instrument into the hand of the surgeon
When the instrument was returned to the student, it was wiped clean of blood and replaced on
the organized table
Needle holder
The student loaded the needle and suture on the needle holder such that when it was passed
to the surgeon, the needle is pointed at the surgeon
The student “snapped” the instrument into the hand of the surgeon
The surgeon was able to use the instrument with minimal adjustment
63
Aseptically Pass Instruments as Circulating Nurse
Surgery Pack
Steri-peel Instrument
The student detached the corners of the steri-peel without contamination or accidentally
opening it too much
The student peeled back the edges of the steri-peel
The student did not roll their wrists
The student stopped when the instrument was exposed enough for the surgeon or scrub
nurse to grasp
The student allowed the surgeon or scrub nurse to control the removal of the instrument
The instrument was not contaminated during the opening process
The student correctly demonstrated set up of a suction machine and described/performed use
and cleaning
The student correctly demonstrated set up of a cautery machine and described/performed use
and cleaning
64
Removal of Sutures
The student accurately recorded all information for dispensing a controlled substance, in a
real or simulated log
Medication Labeling
65
Filling an Oral Solid Medication
The student accurately transcribed the veterinarian’s order onto the appropriate label as defined in
the medication labeling task
The student chose the correct medication (type, concentration and milligram) to fill the order
The student used the correct tools, as provided by the practice, to count out the medication
The student did not handle the medication with their hands
The student counted out the correct account as defined in the veterinarian’s order
The student placed the oral solid medication into the proper container for dispensing
The student accurately transcribed the veterinarian’s order onto the appropriate label as defined in
the medication labeling task
The student chose the correct medication (type and concentration) to fill the order
The student used the correct tools, as provided by the practice, to measure out the proper amount
of medication
The student chose the correct container to hold the volume of medication needed for dispensing
The student measured the correct amount for dispensing as defined in the veterinarians order
The student identified the device needed to administer the liquid medication
The student accurately transcribed the veterinarian’s order onto the appropriate label as defined in
the medication labeling task
The student chose the correct medication (type and concentration) as ordered by the veterinarian
The student chose the correct device to measure out the diluents to be added to the medication
The student chose the correct diluents, as defined by the manufacturer’s instructions
The student followed the manufacturer’s instructions in regards to adding the diluents and
preparation for dispensing
If not provided with the medication, the student identified the device needed to administer the liquid
medication
The student identified, on the label, instructions for shaking and refrigeration if indicated by the
veterinarian or manufacturer
66
Reconstitute a Vaccine
67
Assist in / Perform Prosection on Non-Preserved Animal
~Note: External examination should be done with the DVM present, or the DVM should examine the
animal before proceeding
______ The student placed the animal in lateral or dorsal recumbency and identified species
______ The student correctly identified the following structures during the prosection:
Exposed superficial Aorta
lymph nodes Vena cava
(mandibular, popliteal, Omentum
superficial cervical) Spleen
Jugular veins Pancreas
Mammary glands or Liver
testes Gall bladder
Prepuce and penis Kidneys
(male animal) Adrenal gland
Diaphragm Ureters
Pericardial sac Urinary bladder
Mandible Stomach
Tongue Pyloric region
Larynx Duodenum
Tonsils Jejunum
Esophagus Ileum
Trachea Cecum
Bronchi Colon
Lungs Rectum
Pulmonary vessels Mesentery
Heart
68
Collection, Preservation and Shipping of Samples
______ The student correctly identified when tissues may be pooled in the same jar
______ The student examined the liver, lung, and spleen grossly and collected samples
of lesions and adjacent normal tissue, 1 cm thick and 1 cm x 1 cm square. If the
liver was grossly normal, the student collected a representative sample (1 x 1 x
1 cm)
______ The student examined the kidneys grossly and collected samples of lesions and
adjacent normal tissue in longitudinal wedge sections 1 cm thick. If the kidneys
were grossly normal, the student collected a representative sample (1
longitudinal wedge)
______ The student examined the intestines grossly and discussed special handling of intestinal
samples.
______ The student placed the tissue samples into formalin if indicated
______ The student labeled each sample tissue packet with the following information:
1. Client name or clinician name
2. Animal species
3. Lab that is to test the tissue
4. Date of collection of fresh tissue
5. Site of collection (e.g., liver, right kidney)
______ The student correctly identified the submission forms to use for samples
collected
______ The student learned basic shipping procedures of sending diagnostic samples
______ The student identified all the levels of items (5) needed to ship samples
correctly
______ The student demonstrated the ability to use the ADDL website
______ The student correctly identified two reasons that cause delay of testing
samples
______ The student correctly identified requirements for submission of samples to the
Animal Disease Diagnostic Laboratory
69
Packed Cell Volume
The student mixed by 8-10 gentle inversions a properly collected, anticoagulated (EDTA) tube
of fresh whole blood
The student filled a plain capillary tube 2/3 to ¾ full, wiped the outside of the tube with a
laboratory tissue, and sealed the end of the tube with sealing clay
The student placed the capillary tube in a slot in a microhematocrit centrifuge, sealed end to
the outside edge, and noted the slot number
The student balanced the centrifuge with a balance tube or another patient tube
The student secured the centrifuge lids and covers
The student set the appropriate centrifugation time (and speed where applicable)
After the centrifuge stopped spinning, the student removed the tube and recorded the
presence of hemolysis, lipemia, or icterus
The student obtained results by one of the following methods:
Using a card reader the student aligned the bottom of the red cell column with the zero
percent (0%) line and the top of the plasma with the 100% line
The student read the packed cell volume at the top of the packed red cell column and
recorded the value as a percentage
The instructor verified the test result (within 2% of actual)
The student made all observations and alignments of the capillary tube by viewing from
directly above the reader
The student placed the capillary tube in the groove of the plastic indicator so the intersection
of the clay sealant and the packed red blood cells lined up with the black line, located close to
the center post of the reader
The student rotated the lower metal plate so the 100% line is directly beneath the red line on
the plastic indicator
Keeping the lower metal plate in the same position and using the finger hole in the upper
plate, the student rotated the upper plate so the black spiral line lined up at the top of the top
of the plasma column
The student rotated both the upper and lower plates until the black spiral line lined up at the
top of the red cell column
The student read the packed cell volume from the scale directly beneath the red line on the
plastic indicator and recorded the result as a percentage
The instructor verified the test result (within 2% of actual)
70
Total Protein
The student checked the zero setting and cleanliness of the refractometer
The student mixed by 8-10 gentle inversions, a properly collected, anticoagulated (EDTA)
tube of fresh whole blood
The student filled a plain capillary tube 2/3 to ¾ full, wiped the outside of the tube with a
laboratory tissue, and sealed the end of the tube with sealing clay
The student placed the capillary tube in a slot in a microhematocrit centrifuge sealed end to
the outside edge, and noted the slot number
The student balanced the centrifuge with a balance tube or another patient tube
The student secured the centrifuge lids and covers
The student set the appropriate centrifugation time (and speed where applicable)
After the centrifuge stopped spinning, the student removed the tube and recorded the
presence of hemolysis, lipemia, or icterus
The student broke the hematocrit tube above the buffy coat
Holding the refractometer horizontally, with the cover plate in position on the prism, the
student placed a drop of plasma adjacent to the cover plate, insuring there was no
contamination from the buffy coat, other cellular components, or glass shards. The student
may have enhanced plasma flow by tapping the end of the tube close to the cover plate
The student held the refractometer to their eye with the prism toward the light focused when
necessary, read the total protein value where the dark and bright area intersect and form a
line on the scale, and properly recorded the result
The instructor verified the test result
The student cleaned the measuring prism and cover plate with water and dried them with
laboratory tissue
71
Blood Film Preparation
The student properly mixed, by 8-10 gentle inversions, a properly collected, anti-coagulated
(EDTA) tube of fresh whole blood
The student filled a capillary tube with blood, and from the tube placed a drop of blood, by
touching the capillary tube to the microscope slide, approximately one (1) cm from the frosted
end of the slide
For the handheld method, the student held the microscope slide between their thumb and
index finger with the frosted end toward the index finger
For the table top method, the student held the microscope slide on the outer corner of the
frosted end of the slide, with the frosted end toward their body
While holding the spreader slide at approximately a 30-45 degree angle, the student brought
the spreader slide back into the drop of blood. The blood was allowed to spread along the
edge of the spreader slide, which was then moved forward in an even, forward motion
The student produced a film approximately one-half to two-thirds the length of the slide (~1” in
length)
The student’s blood film was slightly narrower then the width of the slide
The feathered edge of the student’s blood film was relatedly straight across to slightly curved,
and did not end abruptly or have tail-like extensions
When viewed macroscopically, the student’s blood film appeared to have a gradual transition
from the thicker body of the film to the feathered edge
The student’s blood film did not have any lines, holes, scratches, streaks, extensions, or
ridges
The student allowed the blood film to air dry
The student stained the blood film with a rapid stain by initially dipping the blood film for
approximately ten (10) one (1) second dips in the fixative and subsequently making
approximately ten (10) one (1) second dips in Solution One (“eosin”) and Solution Two
(“Methylene blue”)
The student rinsed the back side of the slide with water
The student allowed the blood film to air dry
The student labeled the blood film on the frosted end of the slide with patient ID, species and
date
72
Differential
The student performed a manual white blood cell Unopette® following established laboratory
protocol and properly reported the test results
The student performed a total protein and packed cell volume using established laboratory
protocol, and properly reported the test results
The student performed a differential using a properly stained, quality blood film, including
count and classification of 100 white blood cells, evaluation of RBC, WBC and platelet
morphology and a platelet estimate, and properly reported the results
The student accurately calculated and reported the white blood cell absolute values
The student accurately calculated and reported the mean corpuscular volume (MCV)
The instructor verified the test results
WBC count within 10% of actual
TP within .2g/dl of actual
PVC within 5% of actual
WBC differential percentages within 10% of actual
Platelet estimate within 20% of actual
The student followed the laboratory protocol for the performance of an in-house automated
CBC
The student properly reported the results, including proper units of measurement
The student completed an evaluation of a blood film including evaluation of cellular
morphology, check of the distribution of types of white blood cells, and noted any
discrepancies between the blood film and reported results
The instructor verified the test results
Coagulation Test
The student followed an established protocol for the performance of two different in-house
coagulation tests
The student accurately reported the results, including the proper units of measurement
The instructor verified the test results
Fibrinogen within 200mg/dl of actual
Platelet counts within 20% of actual
The student allowed the serum tubes to adequately clot prior to centrifugation
The student “rimmed” the serum clot tubes prior to centrifugation
The student centrifuged the tubes according to the centrifuge manufacturers
recommendations for speed and time
The student harvested the serum and plasma with a disposable pipette, delivering it into plain,
clean labeled tubes
The condition of the serum or plasma was reported if abnormal (hemolysis, lipemia, or icterus)
74
Chemistry Panel
The student followed the manufacturer’s established protocol for the performance of an in-
house chemistry panel
The student accurately reported the results, including proper units of measurement
The instructor verified the accuracy of the student’s results
The student demonstrated and described the proper care of the patient, collection,
processing, and evaluation for one crossmatch procedure
The student accurately reported crossmatch results using proper medical terminology and
units of measurement
Urinalysis
The student properly performed the physical observation of the urine sample (color, turbidity,
specific gravity with a refractometer), reported the results, including proper terminology and
units of measurement
The student properly performed the chemical analysis of the urine using commercially
available dipstick, and reported the results, including the proper units of measurement
The student prepared (mixed, centrifuged, decanted, applied the sample to a glass slide, etc)
a urine sediment sample then correctly identified and quantified the elements contained in the
urine sediment
The instructor verified the accuracy of the student’s result
75
Serology
The student followed the manufacturer’s established protocol for the performance of in-house
serology tests (ELISA, slide/card agglutination)
The student accurately reported the results, including proper units of measurement
The instructor verified the accuracy of the student’s result
The student used an inoculating loop to collect a sample of bacteria from an isolated colony.
The plate from the previous task may be used
The student used an inoculating loop to apply the primary streak on a blood agar plate
The student rotated the plate and used an inoculating loop to streak the second quadrant of
the blood agar, being careful to overlap the primary streak 1-2 times
The student rotated the plate and used an inoculating loop to streak the third quadrant of the
blood agar, being careful to overlap the second streak 1-2 times
The student inverted the agar plate and placed it in an incubator for 18-24 hours
The student checked the plate after the incubation period for isolated colonies of growth
The student removed the cap of the broth tube with the last two fingers
The student dipped the inoculating needle into the sample
The student stirred the needle in the broth
The student replaced the cap on the tube, labeled the tube, and placed it into the incubator
76
Prepare a Gram-stained Slide
The student selected a bacterial sample by touching a sterile wire or loop to one colony on an
agar plate (did not scoop an entire colony off the agar plate)
The student mixed the sample on a microscope slide with a drop of water or saline
The student circled the sample droplet on the slide with a wax pencil to help identify the area
after staining
The student allowed the slide to air dry
The student heat fixed the slide by passing it through a flame 2-3 times, specimen side up
The student held the slide over the sink or placed it on a rack over the sink, flooded the
smear with crystal violet, and let stand for one minute
The student rinsed the smear briefly with water
The student held the slide over the sink and flooded the smear with Gram’s iodine solution
and let stand for one minute
The student rinsed the smear briefly with water
The student washed the smear with decolorizer until no more purple color washed off (5-10
seconds)
The student rinsed the smear briefly with water
The student held the slide over the sink and flooded the smear with safranin and let stand for
one minute
The student allowed the slide to air dry or gently blotted it dry between paper towels
The student mounted the slide on the microscope and focused on the smear beginning with
the low power lens and working up to the oil immersion lens, and verbally stated Gram
Reaction and morphology
The student selected a bacterial sample by touching a sterile loop or wire to the center of one
colony on an agar plate. If a blood agar plate was used, the student avoided contacting the
agar with the loop or wire
The student applied a drop of 3% hydrogen peroxide onto a microscope slide
The student smeared the sample in the drop of hydrogen peroxide
The student immediately observed the slide for bubbling. If using a loop, the student
observed the loop for bubbling as well
The student showed the slide and verbally identified whether the sample was catalase
positive or negative
The student selected 4-5 morphologically identical colonies from a blood agar or MacConkey plate and
aseptically transferred the selected colonies to a tube of sterile saline with an inoculating loop
The student mixed the contents of the tube by swirling (not inverting) the tube so that the organisms
were uniformly suspended in the saline
The student compared their tube with .5 McFarland nephelometer to ensure that the turbidity in the
tubes was the same
The student adjusted the turbidity in their tube as needed, by dilution or adding of colonies, to attain a
suspension of bacteria with a turbidity equal to the .5 McFarland nephelometer
The student used aseptic technique to dip a sterile cotton swab into the saline suspension of bacteria
The student rotated the swab against the inner wall of the tube to express excess fluid
The student swabbed the entire surface of a Mueller-Hinton agar plate with the swab
The student rotated the plate 60° and re-swabbed the entire surface
The student again rotated the plate 60° and re-swabbed the entire surface, then ran the swab around
the outside edge of the agar
The student applied the antibiotic discs with flamed forceps that were allowed to cool between uses.
The student gently pressed each disc on the agar surface to ensure complete contact, and made sure
the discs were at least 24 mm apart from center to center. The student also ensured that the discs did
not move once contact with the agar surface was made
The student allowed the plate to sit for 1-2 minutes, then inverted the plate and placed it into an
incubator
The student removed the plate from the incubator after 16-18 hours
The student measured the cleared zone diameters in mm
The student recorded the measurements
The student consulted the lab table to determine the susceptibility character of the organism
78
Perform and Read Direct Fecal Smear
79
Perform by Centrifugation and Read Fecal Flotation
The student selected about ¼ teaspoon of feces and placed it in a fecal cup
The student added enough flotation solution to fill the vial about half full
The student mixed the feces into solution with an applicator stick (or equivalent) until no large
fecal particles remained
The student poured the mixture through cheesecloth into a 15 ml centrifuge tube
The student filled the tube with solution until there was a visible meniscus at the top
The student placed a cover slip on top of the vial
The student placed the tube in the centrifuge and spun the mixture for 3-5 minutes at 1300-
1500 rpm
The student carefully removed the cover slip without tilting it and placed it on a microscope
slide
The student placed the slide on a microscope and examined the area of the slide under the
coverslip, and noted and recorded any parasitic material found
The student performed heartworm diagnostic testing according to the clinic SOP for
heartworm testing
The student performed a direct smear and correctly interpreted the result
The student performed an antigen test and correctly interpreted the result
The student performed a Knott’s test and correctly interpreted the result
81
Radiography Safety Procedures
The student accurately recorded all information for radiographs produced on patients, in a real
or simulated log
82
Lateral Thoracic Radiograph
*Note: Small animal thorax and abdomen radiographs must collectively include canine and feline patients
– one case must be a cat, and the other must be a dog. Both views (abdomen or thorax) should be done
on the same case.
*Note: Small animal thorax and abdomen radiographs must collectively include canine and feline patients
– one case must be a cat, and the other must be a dog. Both views (abdomen or thorax) should be done
on the same case.
The student followed standard radiographic safety procedures as defined in the textbook
The student selected a size of cassette appropriate for the pelvis to be radiographed and
appropriately collimated the primary beam to include only the landmarks for the ventrodorsal
pelvis as defined in the textbook
The student selected a detail (slow speed) cassette, or made adjustments for rapid speed
cassettes
The student positioned the animal in dorsal recumbency with the front limbs extended and
secured cranially and the rear limbs extended and secured caudally
The student positioned the animal such that the patella was perpendicular to the x-ray beam
and centered in the trochlear groove and then secured the stifles to maintain this position
The student ensured the rear limbs were parallel to the x-ray table, equally extended and as
close to the table as possible to prevent increased object film distance and/or foreshortening
The student positioned the pelvis such that the pelvic girdle was not angled in relation to the
femurs
The student placed a lead R or L marker next to the corresponding lateral side of the pelvis
The student positioned the animal such that the x-ray beam would be centered between the
coxofemoral joints at the level of the pubic symphysis
The student collimated the primary beam to include the wings of the ilium and the stifle joints
The student placed the calipers at the highest point of the area to be radiographed
The student accurately interpreted the caliper measurement according to manufacturer’s
instructions
If a grid was available, the student placed the cassette in the cassette tray directly beneath the
x-ray table
If no grid was available the student placed the cassette directly on top of the x-ray table, just
beneath the pelvis and adjusted the technique
The student selected the exposure factors according to the previously developed technique
chart for producing a diagnostic radiograph
The student produced the radiograph such that the collimated image included the correctly
chosen limb marker and the landmarks for the ventrodorsal extended pelvic view
No part of the lead glove appeared on the radiograph
85
Lateromedial Projection of the Metacarpophalangeal (Fetlock)
The student selected a size cassette appropriate for the anatomic region to be radiographed, or
collimated to include only landmarks
The student selected a detail cassette
The student positioned the animal squarely so that the weight was evenly distributed on each limb
The student placed the cassette against the medial aspect of the Metacarpophalangeal (fetlock)
The student positioned the primary beam parallel to the floor at appropriate focal film distance, and
centered on the meddle of the metacarpophalangeal (fetlock) joint
The student selected a RF, LF, RR, or LR limb marker according to which limb was being imaged,
and placed the marker just cranial to the metacarpophalangeal (fetlock) joint
The student selected the exposure factors according to the previously developed technique chart
The student produced the radiograph with proper collimation such that only the landmarks for the
entire metacarpophalangeal (fetlock) joint and the marker were included
The student used a portable x-ray machine to produce this image.
The student selected a size cassette appropriate for the anatomic region to be radiographed, or
collimated to include only landmarks
The student selected a detail cassette
The student positioned the animal squarely so that the weight was evenly distributed on each limb
The student placed the cassette against the plantar or palmar aspect of the metatarsophalangeal
or metacarpophalangeal (fetlock)
The student angled the primary beam 20 degrees proximal to distal, at appropriate focal film
distance and centered on the midsaggital plane of the metatarsophalangeal or
metacarpophalangeal (fetlock) joint
The student selected a RF, LF, RR or LR limb marker according to which limb was being imaged,
and placed the marker just lateral to the metatarsophalangeal or metacarpophalangeal (fetlock)
joint of interest
The student selected the exposure factor according to the previously developed technique chart
The student produced the radiograph with proper collimation such that only the landmarks for the
entire metatarsophalangeal or metacarpophalangeal (fetlock) joint and the correct marker were
included
86
Student Name _____________________________________________________
Notes
87
Student Name _____________________________________________________
Notes
88
Student Name _____________________________________________________
Notes
89
Student Name _____________________________________________________
Notes
90