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34 views25 pagesPG Biotechnology Sem 1 and 2 Updated - Compressed
Syllabus related to zoology
Msc
Uploaded by
Devanshu Anil ThoratCopyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
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RASHTRASANT TUKADOJI MAHARAJ NAGPUR
UNIVERSITY, NAGPUR
M.Sc. Biotechnology Syllabus
As per NEP 2020
With Effect from 2023-24
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Scanned with CamScanner ‘SYLLABUS for M. Se. BIOTECHNOLOGY
Choice Based Credit System (Semester Pattern)
Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur
Effective from 2023-2024
ee ee a
M. Se. Biotechnology Semester 1
Paper 1
Cell Biology, Enzymology and Genetics
Course Outcomes:
COM: Comprehend and correlate the structure and function relationship of cells, sub-cellular
organelles, Cellular communication and Cell cycle.
C02: Realize the basic concepts of Enzymology and Enzyme Kinet
‘CO3: Gain familiarity with the concept of Enzyme Engineering ard immobilization.
COA: Develop proficiency in the fundamental molecular principles of genetics and basics of
genetic mapping
UNIT 1: Cell Biology & Signalling
Structure and function of cell organel
Plasma membrane: transport of nutrients, ons and macromolecules.
Cell walls: Archaea, Bacteria, Plant eclls.
Mitochondria: Electron Transport Chain and Oxidative Phosphorylation.
Chloroplasts: Chlorophyll, Carotenoids and Photosynthesis
Golgi complex: Endoplasmic reticulum, Lysosomes, Peroxisomes (functions).
Coll cycle: Molecular events in S. cerevisiae.
Cell signalling: Signal transduction in animal and plant cells (tyrosine kinase, light induced
signalling)
UNIT Ih: Enzymes
Basics Enzymology: Enayme nomenclature, classification and specificity. Concept of
coenzymes.
‘Mechanism of enzyme action: Models, catalysis by proximity effect, acid-base catalysis,
‘electrostatic interaction, metal ion catalysis, nucleophilic and electrophilic catalysis,
Concept of multienzyme complexes: Fatty acid synthase and pyruvate dehydrogenase
complexes.
‘Concept of enzyme regulation: Allosteric (example ATCase), eliemical modification and
calmodulin mediated regulation.
UNIT IIE: Enzyme Kinetics
Basic aspects of enzyme kinetics: Michaelis-Menten equation (derivation, significance and
transformation).
‘Two substrate kinetics: Modifying factors of enzyme kinetics, enzyme
inhibitors.
Enzyme Engineering: Introduction, Principle, steps involved in enzyme engineering,
‘examples and applications of enzyme engineerin
Immobilization of Enzymes: Material used, methods and applications.
oo
ition and types of
Scanned with CamScanner UNIT IV: Genetics
Physical basis of Heredity: Introduction, concepts and theories of Mendelian genetics,
chromosome theory of inheritance, Nucleus, nucleolus and extra chromosomal inheritance.
Population Genetics: Gene pools, allele frequencies, Hardy Weinberg equation, non-random
breeding, genetic dif, gene flow, selection, speciation,
Genetics of bacteria and bacteriophages: Mapping of genes in bacterial and phage
chromosomes by classical genetic crosses; fine structure analysis of a gene; genetic
‘complementation and other genetic crosses using phenotypic markers; phenotype to genotype
connectivity prior to DNA-based understanding of gene
References:
1, Stryer, L. (2015). Biochemistry. (Sth ed.) New York: Freeman.
2. Lehminger, A. L. (2012). Principles of Biochemistry (6th ed.). New York, NY: Worth,
3, Voet, D., & Voet, J. G. (2016). Biochemistry (Sth ed.). Hoboken, NJ: J. Wiley & Sons.
44, Dobson, C. M. (2003). Protein Folding and Misfolding. Nature, 426(6968), 884-890.
dois10.1038/nature02261.
45, Richards, F. M. (1991). The Protein Folding Problem. Scientific American, 264(1), 54-
63. do:10.1038/sciemtficamerican0191-54.
6, Alberts, B., Johnson, A., Lewis, J, Raff, M., Roberts, K., & Walter, P. (2008),
‘Molecular Biology of the Cell (Sth Ed.) New York: Garland Science,
47. Lotish, H. F. (2016). Molecular Cell Biology (8th Bd.). New York: W-H. Freeman.
8. Krebs, J. E., Lewin, B., Kilpatrick, S. T., & Goldstein, E. S, (2014). Lewin's Genes XI,
Burlington, MA: Jones & Bartlett Learning.
9. Cooper, G. M., & Hausman, R. E. (2013). The Cell: a Molecular Approach (6th Ed.).
‘Washington: ASM ; Sunderland.
10. Hardin, 1, Bertoni, G., Kleinsmith, L. J, & Becker, W. M. (2012). Becker's World of
the Cell
11. Bosion (8th Ed.). Benjamin Cummings. 6. Watson, J. D. (2008). Molecular Biology
of the Gene (Sth ed.). Menlo Park, CA: Benjamin/Cummings.
12. David Freifelder. (2004). Microbial genetics. 10th edition, Norosa publisher, New
Delhi.
13. Gardner/Simmons/Snustad, (2006). Principal of Genettes, 8th Edn. John
sons,
14, Klug, W.S..Cummings. (2003). Concepts of genetics. 7h Edn. Pearson Education,
15, Dale, J.W. (1994), Molecular Geneties of bacteria, John Wiley & Sons.
16, Sireips and Yasbin. (2001). Modern microbial Genetics, Niley Lid.
17. John Ringo (2004). Fundamental Genetics. Cembriége University Press.
18, Har, D. L,, & Jones, E, W. (1998). Genetics: Principles and Analysis. Sudbury, MA:
Jones and Bartlet.
19. Pieree, B. A. (2005). Geneties: a Conceptual Approach. New York: W.H, Freeman.
20, Tamarin, R. H., & Leavitt, R. W. (1991). Principles of Genetics. Dubuque, 1A: Wm.
C. Brown.
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Scanned with CamScanner M. Se. Biotechnology Semester I
Paper?!
Biomolecules
Course Outcomes:
CO1: Gain insights into the biochemistry and diversity of Carbohydrates and their
involvement in biological functions.
‘CO2: Comprehend the biochemistry and diversity of Lipids and lipoproteins
CO3: Recognize the importance of Protein structure function relationship and interactions
(C04: Demonstrate the understanding of nucleic acid structure and its dynamics,
UNIT I: Carbohydrates
Carbohydrates chemistry and classification-monosaccherides,
& polysaccharides
Different types of one sugar molecules: Aldoses, Ketoses
Homo polysaccharides: Starch and glycogen (storage polysaccharides), Cellulose and chitin
(Gtructural polysaccharides)
Hetero polysaccharides: Heparin, Hyaluronic acid, Chondroitin-sulphate, Keratin-sulphate
Coll surface molecules - glycolipids, glycoproteins
isaccharides, oligosaccharides
UNIT Mi: Lipids
Classification of lipids
Triacylglycerol, Phospholipids, Glycolipids, Fatty acids, Steroids, Terpenes
(Chemistry and properties of storage and membrane Lipids
‘Types of lipoproteins (LDL, VLDL, HDL, IDL) and chylomicrons
Liposomes
UNIT III: Proteins
Physical and chemical properties of Primary, secondary, tertiary and quatemiary structure
Globular and fibrous proteins, Protein sequencing, Ramachandran plot, Domain structure
Protein folding models and mechanisms
Molten globules, chaperons, Protein misfolding and diseases
Protein -protein interactions
UNIT IV: Nucleic Acids
Chemistry of DNA and RNA
Structure and properties of purines, pyrimidines, nucleosides, nucleotides
A,B, and Z forms of DNA
Models of DNA bending and bendability
Denaturation and renaturation studies of DNA and their applications
Nucleic acid hybridization, Topological structure of DNA.
References: ;
1. Lebninger Principles of Biochemistry by David L, Nelson and Michael M. Cox (rth
edition, 2017). boo
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2, Fundamentals of Biochemistry by V.Voet and J.G. Voet (5th edition available at
hittps:// Wet /fundamentals- listry-by-voct-and-voet-pdf)),
3. Principles of Genetics, Gardner EJ and Sunstad DP, John Wiley and Sons, (8th
cedition,2006)
4. Molecular Biology of the Cell, B. Alberts et al, Garland Publishers, (4th edition,2002)
$. DNA recognition and bending, Rudolf K Allemana and Martin Egli, available at
(htp:/biomednet.com/elecret!1074552100400643)
M. Se. Biotechnology Semester I
Paper 3: Electives
A) Clinical Research © _ ‘er B)Nanobiotechnology
A) Clinical Research
Course Outcomes:
COI: Analyze and evaluate the reporting and reviewing processes of clinical trials, including
the role of legislation and good clinical practice.
(CO2: Apply the principles of informed consent and ethical considerations in the context of
clinical trial design and management.
CO3: Evaluate and address the ethical issues and challenges in clinical trials, including the
‘use of humans in scientific experiments and the role of ethical committees,
C04; Demonstrate an understanding of pharmacovigilance, research governance, and the
process of trial closure.
Unit I Fundamentals of Clinical trials
Fundamentals of clinical trials; Reporting and reviewing clinical trials; Legislation and good
clinical practice; International perspectives; Principles of International Committee on
Harmonisation (ICH)-GCP; CDSCO Guidance.
Unit I Clinical Trial Design and Re
Drug development and trial planning - pre-study requirements for clinical trials; Regulatory
‘approvals for clinical trials; Regulatory submissions; Consort statement; Trial responsibilities
‘and protocols roles and responsibilities of investigators, sponsors and others; Requirements
‘of clinical trial protocols; Legislative requirements for investigational medicinal products,
‘Consent- principles of informed consent; Consent processes; Medical Writing, Clinical Study
Report; Investigational New Drug Application (INDs); Biologies License Application (BLA);
‘Common Technical Document (CTD) for application dossiers
Unit I11 Project Management and Data management
Project management in clinical trials - principles of project management; Application in
clinical trial management; Risk assessment; Research ethics and Bioethics - Principles of
research ethics; Ethical issues in clinical trials; Use of humans in scientific experiments;
Ethical committee system including a historieal overyjaw; informed consent
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Data protection; Legislation and its application.
Unit IV Reporting and reviewing clinical trials and ethics
Quality assurance and governance - quality control in clinical trials; Monitoring and audit;
Inspections; Pharmacovigilance; Research governance; Trial closure and pitfalls trial closure;
Reporting end legal requirements; Common pitfalls in clinical trial management; Adverse
event & serious adverse event reporting; Drug Recall, Introduction to ethical codes and
conduct.
Recommended Textbooks and References:
1, Fundamentals of Clinical Trials. (Authors: Friedman, Lawrence M., Furberg, Curt D.,
DeMets, David; LA; Latest Edition; Publisher: Springer).
2. ‘The Oxford Textbook of Clinical Research Ethics (Authors: Ezekiel J. Emanuel,
Christine C. Grady, Robert A. Crouch et al; Latest Edition; Publisher:Oxford Univ.
Press)
3. ICH guidelines for Good Clinical Practice
(ars /evww. it invPublic_ Web Site/ICH_Products/GuidelinevE
G/E6 RI Guideline.pdf)
4, ICH: Structure and Content of Clinical Study Reports (E3)
5. "Guidance for Industry, ICH M4: Organization of the CTD" U.S. Department of
‘Health and Human Services Food and Drug Administration Center for Drug
Evaluation and Research (CDER) Center for Biologics Evaluation and Research
(CBER) August 2001
6. CDSCO~ Guidance for Industry
B) _Nanobiotechnology
Course Outcomes
‘After successful completion of this Course, students will be able to:
CO 1. Gain insights into the multidimensional attributes of nanotechnology.
CO 2. Appreciate the importance of microelectronics in the field of life sciences.
CO 3. Develop new and exciting cross-disciplinary technologies.
CO 4, Demonstrate the knowledge application of nanotechnology for improving our
everyday life
Unit I Introduction to nanobiotechnology
Introduction to Nanobiotechnology; Concepts, historical perspective; Different formats of
nanomaterials and applications with example for specific cases; Cellular Nanostructures;
‘Nanopores; Biomolecular motors; Bio-inspired Nanostructures, Synthesis and
characterization of different nanomaterials.
Unit I Nano particles and Nano Films
Nanoparticles for drug delivery, concepts, optimization of nanoparticle properties for
suitability of administration through various routes of delivery, advantages, strategies for
sellular internalization and long circulation, strategies forgnhanced permeation through
various anatomical barriers. eo
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Thin films; Colloidal nanostructures; Self Assembly, Nenovesicles; Nanospheres;
‘Nanocapsules and their characterization.
Unit III Nano toxicity
Introduction to Safety of nanomaterials, Basics of nanotoxicity, Models and assays for
‘Nanotoxicity assessment; Fate of nanomaterials in different strata of environme:
Ecotoxicity models and assays; Life cycle assessment, containment, Ethical, safety and
regulatory issues of nanomedicine.
Unit IV Application of Nanobiotechnology
Applications of nanobiocatalysis inthe production of drugs and drug intermediates,
‘Nenoparticles for diagnostics and imaging (theranostics); concepts of smart stimuli
responsive nanoparticles, implications in cancer therapy
‘Nenoarrays for diagnostics, detection of single DNA, self-assembled protein nanoarrays,
Protein nanobiochip nanoparticles for molecular diagnostics, DNA nanomachines,
‘Nenobiosensors, CNT biosensor, DNA nanosensors, Nanowire biosensor, application of
nanodiagnostics.
‘Recommended Textbooks and References:
1. GeroDecher, Joseph B. Scbilenoff, (2003); Multilayer Thin Films: Sequential
Assembly of Nanocomposite Materials, Wiley-VCH Verlag GmbH & Co. KGaA
2. David S. Goodsell, (2004); Bionanotechnology: Lessons from Nature, Wiley-Liss
3. Neelina H. Malsch, Biomedical Nanotechnology, CRC Press
4. Greg T. Hermanson, (2013); Bioconjugate Techniques, (3rd Edition); Elsevier
5._Recent review papers in the area of Nanomedicine.
Sc. Biotechnology Semester I
Paper 4: Research Methodology .
Research Methodology
Course Outcomes
