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CPV Test

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32 views2 pages

CPV Test

Uploaded by

fh4k6zdqxh
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Evaluation of Vcheck Canine Antibody Tests

for the Detection of Protective Antibodies

Key Words: BIONOTE, Vcheck, CPV Ab, CDV Ab, CAV Ab, VN Test, HI Test

Introduction
The canine viruses which cause distemper, according to manufacturer’s instructions. The
par voviral enteritis, and infectious hepatitis Vcheck result of medium (3, 3.5) or high titer (from
have a high correlation between the presence of 4 to 6) is considered having a ‘high’ protective
antibody and protective immunity. Core vaccines antibody, while one of negative (0) or low titer
are recommended for all puppies and dogs with an (1, 2) is considered having a ‘low’ protective
unknown vaccination history. These core vaccines antibody. They were also referred to CUCVM for
include: canine distemper virus (CDV), canine Virus Neutralization (VN) test and evaluated with
adenovirus (CAV) and canine parvovirus type 2 (CPV- a commercial in-practice test (product 'I’). A titer
2). It is recommended to use an in-house serological result of 1:32 (1:16) or greater is considered as ‘high’.
testing for antibodies specific for vaccine antigens
following vaccination.

The Vcheck CPV, CDV or CAV Ab Test is a one- Results


step rapid test for the semi-quantitative detection The Vcheck antibody tests demonstrated higher
of antibodies to parvovirus, distemper virus or sensitivities and specificities than commercially
adenovirus in canine serum or plasma. The purpose available ‘I’ kit, compared against the reference
of this study is to verify the performance of the tests; The CPV Ab Test showed 100% sensitivity and
Vcheck compared to the gold standard test for CPV, 85.7% specificity, CDV Ab Test 100 and 83.1%, CAV
CDV and CAV antibody titers. Ab Test 87.8 and 98.2%. On the contrary, ‘I’ kit had
95.9% sensitivity and 71.4% specificity in CPV Ab,
97.1 and 79.7% in CDV Ab, 84.8 and 92.7% in CAV Ab

Materials and Methods (Refer to Table 1, 2 and 3).

CPV Ab titer test:

Conclusion
A total of 56 random canine serum samples were
tested by Vcheck CPV Ab test kit according to
manufacturer’s instructions (BioNote, Korea). The The findings of the present study indicated that
Vcheck result of medium (3, 3.5) or high titer (from the Vcheck showed higher correlation with the
4 to 6) is considered having a ‘high’ protective gold standard tests (HI, VN test) than or equal to a
antibody, while one of negative (0) or low titer (1, commercial product 'I', so it can be used as a useful
2) is considered having a ‘low’ protective antibody. method of serological testing due to its rapidity and
They were also referred to Cornell University ease of performance, providing accurate antibody
College of Veterinar y Medicine (CUCVM) for titer results against CPV, CDV and CAV in-house.
Hemagglutination Inhibition (HI) test and evaluated
with a commercial in-practice test (product 'I’). A
titer result of 1:80 or greater is considered as ‘high’.

CDV Ab (CAV Ab) titer test:


Reference
A total of 129 (219) random canine serum samples
1. WSAVA GUIDELINES FOR VACCINATION OF DOGS AND CATS,
were tested by Vcheck CDV Ab (CAV Ab) test kit Journal of Small Animal Practice – Vol 57, January 2016
Commercial ‘I’ kit Vcheck CPV Ab
Comparative
Total Total
Evaluation
High Low High Low

HI Test High 47 2 49 49 0 49
(Cornell
Univ.) Low 2 5 7 1 6 7

Total 49 7 56 50 6 56

Sensitivity 95.9% (47/49) 100% (49/49)

Specificity 71.4% (5/7) 85.7% (6/7)

Overall Agreement 92.9% (52/56) 98.2% (55/56)

Table 1. Correlation of Vcheck CPV Ab test and a commercial ‘I’ kit with HI test

Commercial ‘I’ kit Vcheck CDV Ab


Comparative
Total Total
Evaluation
High Low High Low

VN Test High 68 2 70 70 0 70
(Cornell
Univ.) Low 12 47 59 10 49 59

Total 80 49 129 80 49 129

Sensitivity 97.1% (68/70) 100% (70/70)

Specificity 79.7% (47/59) 83.1% (49/59)

Overall Agreement 89.1% (115/129) 92.2% (119/129)

Table 2. Correlation of Vcheck CDV Ab test and a commercial ‘I’ kit with VN test

Commercial ‘I’ kit Vcheck CAV Ab


Comparative
Total Total
Evaluation
High Low High Low

VN Test High 139 25 164 144 20 164


(Cornell
Univ.) Low 4 51 55 1 54 55

Total 143 76 219 145 74 219

Sensitivity 84.8% (139/164) 87.8% (144/164)

Specificity 92.7% (51/55) 98.2% (54/55)

Overall Agreement 86.8% (190/219) 90.4% (198/219)

Table 3. Correlation of Vcheck CAV Ab test and a commercial ‘I’ kit with VN test

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