MYCOBACTERIUM MOCK EXAM QUESTIONS

BACTE-LEC

Based on the provided document, here are 50 multiple-choice questions for an exam:
1. Which medium is used to culture Mycobacterium tuberculosis?
o A. Blood agar
o B. Chocolate agar
o C. Lowenstein-Jensen
o D. MacConkey agar
2. What is the main decontamination agent used for Mycobacteria?
o A. Ethanol
o B. NaOH
o C. Hydrogen peroxide
o D. Acetic acid
3. Which stain is used for Mycobacterium tuberculosis?
o A. Gram stain
o B. Crystal violet
o C. Ziehl-Neelsen
o D. India ink
4. What is the diagnostic test for detecting M. tuberculosis in the skin?
o A. ELISA
o B. PCR
o C. Mantoux test
o D. Western blot
5. Which mycobacterium is associated with leprosy?
o A. Mycobacterium tuberculosis
o B. Mycobacterium leprae
o C. Mycobacterium avium
 o D. Mycobacterium bovis
6. Which mycobacterium is part of the MAI complex?
o A. Mycobacterium kansasii
o B. Mycobacterium marinum
o C. Mycobacterium avium-intracellulare
o D. Mycobacterium terrae
7. What is the Runyon classification based on?
o A. Pigmentation and growth rate
o B. Cell wall structure
o C. Antibiotic resistance
o D. Pathogenicity
8. Which mycobacterium is a photochromogen?
o A. Mycobacterium avium
o B. Mycobacterium kansasii
o C. Mycobacterium leprae
o D. Mycobacterium tuberculosis
9. Which test differentiates Mycobacterium tuberculosis from Mycobacterium bovis?
o A. Niacin test
o B. Catalase test
o C. Urease test
o D. Indole test
10. What is the characteristic staining of Nocardia?
o A. Partially acid-fast
o B. Gram-negative
o C. Spore staining
o D. Non-acid fast
11. Which bacterium causes rat-bite fever and is referred to as SODUKU?
o A. Leptospira interrogans
 o B. Treponema pallidum
o C. Spirillum minus
o D. Borrelia burgdorferi
12. Which Borrelia species causes Lyme disease?
o A. Borrelia recurrentis
o B. Borrelia burgdorferi
o C. Borrelia parkeri
o D. Borrelia hermsii
13. Which vector is associated with Borrelia burgdorferi?
o A. Lice
o B. Fleas
o C. Ticks
o D. Mosquitoes
14. What is the severe form of leptospirosis known as?
o A. Typhus
o B. Weil’s disease
o C. Lyme disease
o D. Rocky Mountain spotted fever
15. Which serovar of Leptospira interrogans causes icterohemorrhagic leptospirosis?
o A. Canicola
o B. Icterohemorrhagiae
o C. Autumnalis
o D. Grippotyphosa
16. Which disease is caused by Chlamydia trachomatis serovars L1, L2, and L3?
o A. Trachoma
o B. Inclusion conjunctivitis
o C. Lymphogranuloma venereum
o D. Psittacosis
17. Which medium is used for the culture of Chlamydia trachomatis?
o A. McCoy cells
o B. Blood agar
o C. Chocolate agar
o D. MacConkey agar
18. What is the diagnostic test for Chlamydia trachomatis that involves direct examination?
o A. ELISA
o B. PCR
o C. DFA (Direct Fluorescent Antibody)
o D. Western blot
19. Which species of Mycoplasma is associated with respiratory infections?
o A. Mycoplasma hominis
o B. Mycoplasma pneumoniae
o C. Ureaplasma urealyticum
o D. Mycoplasma genitalium
20. Which medium is used to culture Mycoplasma pneumoniae?
o A. Blood agar
o B. Chocolate agar
o C. SP4 broth
o D. MacConkey agar
21. Which Rickettsia species causes Rocky Mountain spotted fever?
o A. Rickettsia akari
o B. Rickettsia prowazekii
o C. Rickettsia rickettsii
o D. Rickettsia typhi
22. What is the vector for Rickettsia prowazekii?
o A. Ticks
o B. Lice
 o C. Fleas
o D. Mites
23. Which agent causes Q fever?
o A. Rickettsia typhi
o B. Coxiella burnetii
o C. Ehrlichia chaffeensis
o D. Bartonella henselae
24. Which test is used for the laboratory diagnosis of Rickettsia species?
o A. ELISA
o B. Weil-Felix reaction
o C. Western blot
o D. PCR
25. Which Bartonella species is the primary causative agent of cat scratch disease (CSD)?
o A. Bartonella quintana
o B. Bartonella bacilliformis
o C. Bartonella henselae
o D. Bartonella clarridgeiae
26. Which Chlamydia species causes pneumonia and is associated with Guillain-Barre
syndrome?
o A. Chlamydia trachomatis
o B. Chlamydophila pneumoniae
o C. Chlamydophila psittaci
o D. Chlamydia suis
27. Which Chlamydia species is acquired from birds and causes psittacosis?
o A. Chlamydia trachomatis
o B. Chlamydophila pneumoniae
o C. Chlamydophila psittaci
o D. Chlamydia suis
28. Which Mycoplasma species is associated with genital infections and is urease positive?
 o A. Mycoplasma pneumoniae
o B. Ureaplasma urealyticum
o C. Mycoplasma genitalium
o D. Mycoplasma hominis
29. Which medium is used for the culture of genital mycoplasmas?
o A. Blood agar
o B. Chocolate agar
o C. A7 or A8 agar
o D. MacConkey agar
30. Which test is used to differentiate Mycoplasma hominis from Mycoplasma pneumoniae?
o A. Arginine utilization
o B. Glucose fermentation
o C. Urease test
o D. Indole test
31. Which disease is caused by Rickettsia typhi?
o A. Rocky Mountain spotted fever
o B. Murine typhus
o C. Q fever
o D. Trench fever
32. What is the principal vector for Orientia tsutsugamushi, the causative agent of scrub
typhus?
o A. Ticks
o B. Lice
o C. Chiggers
o D. Fleas
33. Which bacterium causes trench fever?
o A. Rickettsia prowazekii
o B. Coxiella burnetii
o C. Bartonella quintana
 o D. Rickettsia akari
34. Which medium is used to culture Mycobacterium leprae?
o A. Lowenstein-Jensen
o B. Petragnani
o C. American Thoracic Society
o D. It cannot be cultured in artificial media
35. Which test detects the presence of pyrazinamidase activity in mycobacteria?
o A. Catalase test
o B. Pyrazinamidase test
o C. Urease test
o D. Indole test
36. Which Mycobacterium species is known to be urease positive?
o A. Mycobacterium tuberculosis
o B. Mycobacterium leprae
o C. Mycobacterium kansasii
o D. Mycobacterium marinum
37. Which method is commonly used to stain Mycobacterium leprae in tissue smears?
o A. Gram stain
o B. Fite-Faraco’s stain
o C. India ink
o D. Crystal violet
38. Which bacterium is also known as Koch's bacillus?
o A. Mycobacterium leprae
o B. Mycobacterium tuberculosis
o C. Mycobacterium bovis
o D. Mycobacterium kansasii
39. Which of the following is a common method for the decontamination and digestion of
mycobacteria?
o A. Ethanol and hydrogen peroxide
 o B. N-acetyl-L-cysteine (NALC) and NaOH
o C. Sodium chloride and trisodium phosphate
o D. Dithiothreitol and acetic acid
40. Which medium is commonly used for the culture of mycobacteria?
o A. MacConkey agar
o B. Lowenstein-Jensen medium
o C. Blood agar
o D. Sabouraud dextrose agar
41. What staining method is used for the detection of acid-fast bacilli (AFB)?
o A. Gram stain
o B. Ziehl-Neelsen stain
o C. Crystal violet stain
o D. India ink stain
42. Mycobacterium leprae is diagnosed using smears of tissue juice and which stain?
o A. Gram stain
o B. Acid-fast stain
o C. Giemsa stain
o D. Methylene blue stain
43. Which mycobacterium is part of the Mycobacterium avium-intracellulare complex
(MAI)?
o A. Mycobacterium tuberculosis
o B. Mycobacterium kansasii
o C. Mycobacterium avium
o D. Mycobacterium leprae
44. Which Runyon group includes rapid-growing mycobacteria?
o A. Group 1
o B. Group 2
o C. Group 3
o D. Group 4
45. Which reagent is used for the niacin test in mycobacteria?
o A. Sulfuric acid
o B. Cyanogen bromide
o C. Sodium hydroxide
o D. Hydrochloric acid
46. Tween 80 hydrolysis is most useful in the identification of which mycobacterium?
o A. Mycobacterium tuberculosis
o B. Mycobacterium leprae
o C. Mycobacterium kansasii
o D. Mycobacterium avium
47. What is a positive result for the urease test in mycobacteria?
o A. Production of ammonia
o B. Production of hydrogen sulfide
o C. Production of nitrites
o D. Production of indole
48. Which mycobacterium is known to grow in 5% NaCl?
o A. Mycobacterium leprae
o B. Mycobacterium tuberculosis
o C. Mycobacterium fortuitum
o D. Mycobacterium bovis
49. Actinomyces is known to be:
o A. Gram-negative
o B. Gram-positive
o C. Acid-fast
o D. Non-acid-fast
50. Which bacteria is partially acid-fast and aerobic?
o A. Nocardia
o B. Actinomyces
o C. Tropheryma whipplei
o D. Spirillum minus