Benchtop Meter

PHS-W Series
User Manual

BIOBASE GROUP

Version 2021.04
Introduction
Thank you for selecting the PHS-W series benchtop pH meter. This manual provides a step-by-step
guide to help you operate the meter, please carefully read the following instructions before use.
Unpacking
Before unpacking, ensure that the current work environment meets following conditions.
● Relative humidity is less than 80%.
● Ambient temperature is greater than 0℃ and less than 60℃.
● No potential electromagnetic interference.
The following list describes the standard components of the meter. After the unpacking, please check
all components are complete. If any are damaged or missing, please contact nearest distributor.

pH Meter Electrode Arm pH4.01/7.00/10.01 Buffer Pouches

pH Electrode Temperature Probe (Only for PHS-3BW) DC9V Power Adapter

Display
PHS-W series benchtop pH meter is equipped with an easy-read LCD display that used to show
measured values and mode icons. The following table describes the function of each icon.

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INDEX:
Measurement mode icon: Electrode slope icon:
Indicates the meter is in the Indicates the average slope
measurement mode. of the pH electrode.

Calibration mode icon: Error alarm icon:

Indicates the meter is in the Indicates the pH buffer or
calibration mode. electrode has expired.

Temperature setting icon: Automatic Temperature

Indicates the meter is in the Compensation:
temperature setting mode. Indicates the temperature
compensation is enabled.

Keypad
The meter has a succinct membrane keypad, names and symbols describe the each function key
controls.
KEY FUNCTION
ON Switches the meter ON/OFF.
CAL Starts calibration.
pH Enters the pH measurement mode.
mV Enters the mV measurement mode.
℃ Sets the temperature.
Increase the setting value.
Decrease the setting value.

ENTER Confirms the calibration or settings.

Connectors
PHS-W series benchtop pH meter provides 3 connectors are located on the rear panel. Listed in the
below table are the details of these connectors.

NO. CONNECTOR DESCRIPTION

1 ELECTRODE Used for connecting the pH or ORP electrode.
2 ATC Used for connecting the temperature probe (Only for PHS-3BW meter)
3 POWER Used for connecting the power adapter.

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Installing the Electrode Holder
Take out the electrode arm from the packaging. The base plate of the electrode holder has an
irregular round hole, the electrode arm has a connecting rod.
Insert the connecting rod into the irregular round hole and swivel the electrode arm 90o . Electrode
holder is now ready to swing into desired position.

Adjustment of Electrode Arm

After installation, if the electrode arm automatically rises or falls, you need to adjust the screws until
arm locate at any position.
1. Remove the plastic cover from the electrode arm.
2. Use the screwdriver to tighten the screw moderately.
3. Insert the plastic cover to previous position. Installation is completed.

Connecting the Electorde

1. Take out the pH electrode from the packaging. Follow the steps below to place the electrode into
left or right side of the electrode arm.

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2. Insert the BNC connector into the connector socket labeled “ELECTRODE”. Rotate and push the
connector clockwise until it locks. After the connection is completed, DO NOT pull on the cable.
Always make sure that the connector is clean and dry

Connecting the Power Adapter

1. Before plugging in the power adapter, ensure that its voltage matches the local main voltage.
2. Insert the connector to the power socket. The meter is now ready for use.

Preparing the pH Buffer Solutions

PHS-W series benchtop pH meter is packaged with three pH buffer packets required for calibration.
● Open the pH7.00 buffer packet, place the powder into a 250ml volumetric flask. Pour distilled
water 250ml to scale line, mix the solution until the reagent is completely dissolved.
● Preparation of pH4.01 and 10.01 standard buffer solutions are the same as above.
● Prepared standard buffer solutions should be stored in hermetically sealed glass containers.

Prior to Use
Remove the protective cap from the bottom of the electrode. If the glass sensitive membrane has
dried out, soak the electrode in 3M KCL solution (pH adjusted to 4.0) for at least 15 minutes. DO
NOT store the electrode in distilled or deionised water that will render the electrodes useless.

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Power On/Off
● Press the ON key to turn on the meter, the display shows the measured value.
● Press and hold the ON key for 5 seconds, the meter will turn off.
Temperature Compensation
For better accuracy, we recommend that using a temperature probe for the calibration and
measurements.
Manual Temperature Compensation
1.1 Use a high-accuracy thermometer to measure the temperature of sample.
1.2 Press the oC key to enter the temperature setting mode.
1.3 Press the or key to set the temperature value.
1.4 Press the Enter key to confirm, the meter returns to the measurement mode. Setting is
completed.

Automatic Temperature Compensation (Only for PHS-3BW meter)

2.1 The PHS-3BW benchtop pH meter is supplied with a TP-100K temperature probe. Place the
temperature probe into the round hole of the electrode arm.

2.2 Insert the phone plug to the connector socket labeled “ATC”. Ensure the connector is fully seated.
When the connection is done, the “ATC” indicator will show on the display. The meter is now
switched to the automatic temperature compensation mode.

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pH Calibration
PHS-W series benchtop pH meter can perform 2 points calibration. The first calibration point must
be pH7.00. If your sample is belong to acidic substances, select the pH4.01 buffer solution as second
calibration point, if the alkaline substances, select the pH10.01 buffer solution. The meter will
automatically recognize and calibrate to following standard buffer values.
USA Standard Buffers pH4.01, 7.00, 10.01
NIST Standard Buffers pH4.01, 6.86, 9.18
The meter must be calibrated prior to first use or new electrode replaced. To ensure accuracy, regular
calibration is recommended. DO NOT reuse the pH buffer solution after calibration, contaminants in
solution will affect the calibration and eventually the accuracy of the measurement.
1. Rinse the pH electrode with distilled water. Press the Cal key, the meter enters the calibration
mode, the display shows “CAL/pH7.00”.

2. Place the pH electrode into the pH7.00 standard buffer solution, the end of the electrode must be
completely immersed into the calibration solution.
Stir the electrode gently to create a homogeneous solution.
3. Press the Enter key, the meter begins the calibration. Wait for the reading to stabilize, the
displayed value will flash 3 times indicating that first calibration point is completed.

The display automatically shows “---” and waits for calibrating the second point.

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4. Rinse the pH electrode with distilled water. Place the electrode into the pH4.01 (or 10.01) standard
buffer solution. Press the Enter key, the meter automatically recognizes the pH buffer solution and
begins the calibration (e.g., pH4.01)

5. Wait for the reading to stabilize, the displayed value will flash 3 times, the meter automatically
shows electrode slope and returns to the measurement mode. Calibration is completed.

Exit the Calibration

During the calibration process, press the pH key, the meter will exit the calibration and return to the
measurement mode.

● The electrode icon ( ) shows the average slope of the pH electrode when the calibration is
completed. If the slope or calibration result does not meet measurement requirements, the icon will
disappear on the display.
● If the meter shows “ERR” on the display, please check the pH electrode and ensure the pH buffer
solutions are fresh and uncontaminated.
pH Measurement
1.1 Press the pH key to enter the pH measurement mode.
1.2 Rinse the pH electrode with distilled water to remove any impurities adhering to the probe body.
1.3 Place the electrode into the sample solution, stir the electrode gently.

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1.4 Record the measured value when the reading is stable.
mV Measurement
2.1 Press the mV key to enter the mV measurement mode.
2.2 Rinse the electrode thoroughly with distilled water.
2.3 Place the electrode into the sample solution, stir the electrode gently.
2.4 Record the measured value when the reading is stable.
pH Electrode Care and Maintenance
Since pH electrode is susceptible to dirt and contamination, clean as necessary depending on the
extent and condition of use.
● After measuring: rinse the electrode in distilled water, store the electrode into the 3M KCL
solution.
● Salt deposits: soak the electrode in warm tap water to dissolve deposits, then thoroughly rinse with
distilled water.
● Oil or Grease film: wash the glass sensitive membrane of electrode gently in some detergents and
water. If necessary, using the alcohol to clean the
sensitive membrane, then rinse with distilled water. Place the electrode in the 3M KCL solution for
at least 30 minutes.
● Clogged reference junction: heat a diluted KCl solution to 60o C to 80o C. Place the electrode into
the heated solution for about 10 minutes. Allow the
electrode to cool in some unheated KCl solution.
● Protein deposits: prepare a 1% pepsin solution in 0.1M of HCL. Place the electrode in the solution
for 10 minutes. Rinse the electrod with distilled water.
Rectivating the Electrode
If stored and cleaned properly, the electrode should be ready for immediate use. However, a
dehydrated sensitive membrane may cause sluggish response.
To rehydrate the sensitive membrane, immerse the electrode in a pH4.01 buffer solution for 10 to 30
minutes. If this fails, the electrode requires activation.
1. Soak the electrode in 0.1M HCl for 5 minutes.
2. Remove and rinse with deionized water, then place in 0.1M NaOH for 5 minutes.
3. Remove and rinse again, and soak in 3M KCL solution for at least 30 minutes.
Optional pH Electrodes
PHS-W series benchtop pH meter comes with a general purpose pH electrode that is used to measure
the pH of the liquids. If this electrode can not meet your measurement requirements, please refer to
the table below to select an applicable probe.

SAMPLE P11 P12 P13 P15 P16 P18 P19 P21 E201 E202
TYPE
Agar ●
Beer ● ● ● ● ● ●
Blood Products ● ● ● ● ●
Bread, Dough ● ●
Cement ●
Cosmetics ● ● ● ● ● ●
Dairy Products ● ● ● ● ●

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 Education ● ● ●
Fats/Cream ●
Field Use ● ● ●
Fish Products ● ●
Lab Flasks ●
Low Ionic ● ● ●
Meat, Cheese ● ●
Micro Samples ●
Paint ● ● ●
Photographic
Soil ● ●
Surface ●
Test Tubes ● ●
Tris Buffer ●
Viscose ●
Samples
Troubleshooting
LCD DISPLAY CAUSE CORRECTIVE ACTION
Electrode dried out Soak the pH electrode in 3M KCL solution at least
20 minutes.
Measured value is out of range Check the electrode whether clogged, dirty or
broken.
Incorrect pH buffer solutions Using the fresh pH buffer solutions for calibration.
ERR Electrode has expired Replace the pH electrode.
Specifications
Model PHS-25CW PHS-3BW
Range 0.00~14.00pH
Accuracy ±0.05pH ±0.01pH
Resolution 0.01pH
Calibration Points 2 points
pH Calibration Solutions USA (pH4.01/7.00/10.01)
Temperature Compensation 0~100°C, Manual 0~100°C, Automatic or
Manual
Range -1999~1999mV
Accuracy ±10mV ±1mV
mV Resolution 1mV
Range --- 0~100°C
Accuracy --- ±1°C
Temperature Resolution --- 1°C
Connector BNC
Power Requirements DC9V, using AC adapters, 220VAC/50Hz
Others Dimensions 210(L)×205(W)×65(H)mm
Weight 1.5kg

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Warranty
The warranty period for meter is one year from the date of shipment. Above warranty does not cover
sensor and calibration solutions. Out of warranty products will be repaired on a charged basis. The
warranty on your meter shall not apply to defects resulting from:
● Improper or inadequate maintenance by customer.
● Unauthorized modification or misuse.
● Operation outside of the environment specifications of the products.
For more information, please contact the nearest authorized distributor.

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BIOBASE GROUP
2# building, No.9 Gangxing Road, High-tech Zone, Jinan City, Shandong Province,
China
Tel: +86-531-81219803/01
Fax: +86-531-81219804
Inquiry: [email protected]
Complaints: [email protected]
After-sales service: [email protected]; [email protected]
Web: www.biobase.cc/www.meihuatrade.com / www.biobase.com