Jinan Biobase Biotech Co., Ltd.

User Manual

For

BKC-DL6MB

Large Capacity
Refrigerated Centrifuge
 Jinan Biobase Biotech Co., Ltd.

Thanks for using our instrument！For your fast and

sincerely service please read the manual carefully when
you use the machine.

Our duty is at your service！

Our pursuit is satisfying you！

Our motto：

Credit based

Quality first
 Jinan Biobase Biotech Co., Ltd.

Safety Notices
We appreciate your business with BKC-DL6MB centrifuge. To prevent any

potential accident, please operate centrifuges according to the following safety

notices.

1. Unplug the main power cord, when performing maintenance or when

centrifuge is expected not being used for a long period of time.

2. Load the rotor with samples arranged symmetrically. Opposing tubes

must be of equal weight. If necessary, use "water blank" tubes to balance

sample tubes of unequal weigh. Do not conclude that tubes are balanced

by sight over volume. Use the pan balance provided in the centrifuge

room for balancing tubes in rotors for the centrifuge.

3. Never exceed the maximum speed posted for the rotor!

4. Never use the rotor that appears damaged (e.g. O-rings missing,

scratched, corroded, and cracked).

Thank you!
Jinan Biobase Biotech Co., Ltd.
 Jinan Biobase Biotech Co., Ltd.

The Instruction Manual for BKC-DL6MB large capacity refrigerated

centrifuge contains all of the information needed to install, operate, and
maintenance. To prevent any potential accident, please read this User Manual
carefully and operate centrifuges according to the following safety protocol.
The terms danger, warning, caution and note have specific meaning in this
manual.
Danger advises against certain actions or situations that could result in
personal injury.
Warning advises against certain actions or situations that could result in
personal injury, health risk and environment pollution.
Caution advises against actions or situations that could damage
instrument, produce inaccurate data, or invalidate a procedure.
Note provides useful information regarding an operation, function, or
procedure.

The signs of danger, warning and caution and note are

placed in the left of corresponding text.

Part 1 Introduction
BKC-DL6MB large capacity refrigerated centrifuge is an ideal centrifugal
instrument widely used in cosmetic surgery, laboratory, blood bank and
hospital.

1.1 Application and Features

The BKC-DL6MB centrifuge can be equipped with large capacity rotor.
The Max Speed is 6000rpm, Max RCF is 6600xg and the Max Capacity is
6x1000ml. It is the usual instrument for separating and producing.
Brushless frequency motor, LED(digital) display which indicates the speed,
 Jinan Biobase Biotech Co., Ltd.

time and temperature.

Frame is 3 tiers protection steel jacket, and with the stainless steel
chamber. Automatically electronic lock and pneumatic spring and automatic
locked cover can assure the security, the lid cannot open in order to protect the
operator in operation.
Small vibration,low noise and beautiful design. Adopt advanced CPU
control system realizing microprocessor control, it can control rotate speed and
relative RCF and LED display.
The centrifuge has following functions:
a. It can store all the parameters automatically in operation, and can use
directly when startup next time.
b. When exceeding max speed with 500r/min, it will stop automatically
and protect the system.
c. There are 10 kinds of acceleration and 10 kinds of deceleration.
d. Rotor pre-cooling
This centrifuge is in accord with national standard GB4793.7-2001 and
international standard IEC61010-2-20.

1.2 Main technical parameters for BKC-DL6MB:

Max speed： 6000rpm Max RCF： 6600xg
Max volume： 6x1000ml Noise： ≤60dBA
Timer： 1～9h59min Net weight： 310KG
Dimension(LxWxH)： 800mm×740 mm×930mm Power supply： AC 220V 50-60Hz 25A
Speed accuracy： ±20rpm Temperature range -20~40°C
Temperature accuracy ±1°C Package： wooden box
Speed accuracy：
 Jinan Biobase Biotech Co., Ltd.

1.3 Matched Rotors for BKC-DL6MB

Max Speed Capacity Max RCF
Rotor No Rotor Type
（r/min） （ml） （×g）
30223 Angle Rotor 6000 6×500 6600
30298 Swing Rotor 4200 6×1000 5100

Part 2 Installation

2.1 Unpacking the centrifuge

a) Check the package before opening the packing box
b) Examine the Centrifuge for any shipping damage. If any
damage was found, please contact our service department.

2.2 Instrument Installation

1. The work table should be smooth and stable, the four feet of the
centrifuge should touch the surface of the work table firmly

Warning In order to ensure safety, please keep 30cm space

around the instrument, and stay out of the safety space in
operation, do not store any inflammable and any other dangerous
goods in the safety space.
2. Electrical source should be 220V Single phase, with independent earth
line
Danger! Error voltage or the voltage over 10% will
damage the instrument. You need to check the voltage before
connect the power.

3. This instrument only can be used indoor and it is better to operate under
20℃ (constant temperature) condition. maximum relative humidity is 80% for
 Jinan Biobase Biotech Co., Ltd.

31℃, while 50% for 40℃, and avoid placing the centrifuge under heat
producer(e.g sunshine、heating pipe and radiator)

Caution! In order to assure ventilation effect, you should

keep enough space for centrifuge devices. Overheating and
poorly ventilated room will damage the instrument.

4. Use the attached power cord

Danger Centrifuge rear over with power socket ！ ,the
! security identity is enclosed. Socket is 220V 25A,
be careful when connecting the socket.

Part 3 Operation

The operation of BKC-DL6MB large capacity centrifuge includes power on,

lid opening, rotor installment, lid closing, operation, start the centrifuge, stop the
centrifuge, these 7 steps. Here is the detailed description.

3.1 Power on
Power switch is located on the left bottom corner of control panel. Turn it to
―ON‖ position, the compressor start to work and the centrifuge is power on.
Caution Side of this power switch is posted with safety
marking this means the power is 220V, It is
dangerous voltage, beware of electric shock.
3.2 Lid opening
There is OPEN key on the control panel, press OPEN, lock opened. push
the lid upward until it open completely, Spring level will help to open the lid.
 Jinan Biobase Biotech Co., Ltd.

3.3 Rotor installment

（1） Load the rotor to the central adapter located on the axle of
electrical motor, Fasten the screws in the central sleeve till the rotors are
installed. Use the special wrench to fasten the screws after installing the rotors
in the central sleeve of the axle.
Warning Check the rotor before loading. Never use
rotors showing signs of cracking or corrosion, never use expired
rotors.
（2）Fill the tubes equally by eye (about 75% of the total volume),
balance the tubes by scale.
Warning The difference in weight between the tubes
should not exceed 2 grams. Load the tubes symmetrically
The input centrifugal tubes should be even.
Large difference may be cause big shaking in running. In this case, the
centrifuge should be stopped for checking. The tubes should be placed
symmetrically by even number. The tubes input improper、 unserious weigh the
tubes and imbalance working will result in accidents.
Note ⅰ. Only the specified rotors can be used
ⅱ . Never use the rotor that appears damaged. Please
confirm all the rotors、buckets and other accessories before use
it.
ⅲ．Never exceed the maximum speed of the rotor and the cups!

3.4 Closing the lid

After the rotor has been properly loaded, close the centrifuge lid

3.5 Operation
 Jinan Biobase Biotech Co., Ltd.

1) Explanation for control panel

The time and speed will be shown on the panel, the control panel as
shown in Chart 1：
Chart 1

A、 P Window
This Window displays the program, there are 0-9 these 10 programs for
your choice.
B、 NO. Window
This window displays Rotor No., when setting the Rotor No., it is the last
two numbers of the rotor. For example, the Rotor No. of 6x1000 swing
rotor is 30298, it will display the last two numbers 98
C、 SPEED Window
This window can display both SPEED and RCF, press the ―RCF‖ button
below this window, it can transfer from SPEED to RCF, or transfer RCF to
SPEED.
D、 TIME Window
This window displays the timer, max timer is 9h59min, and the time
counts down during the operation.
E、 TEMP Window
The window displays two points: ①It mainly displays temperature. When
the temperature above 0℃, it will change by 0.5 ℃. When the temperature
below 0℃, it will change by 1 ℃
②It also can display the errors. All the errors are shown in the following
 Jinan Biobase Biotech Co., Ltd.

table
Error Displaying
frequen
frequency
Temp cy Speed
changer Lid is not Imbalanc Over Over Settin
Error Measur changer sensor
communicatio closing e Speed Temp g error
e operatio error
n error
n error
Display E-0 E-1 E-2 E-3 E-4 E-5 E-6 E-7 E-9

F、 A Window
This window displays acceleration. There are 0-9 these 10 acceleration
for your choice. 0 is constant speed, 1 is the lowest acceleration and 9 is
the fastest acceleration.
G、 D Window
This window displays deceleration. There are 0-9 these 10 deceleration
for your choice. 0 is constant speed, 1 is the fastest deceleration and 9 is
the lowest deceleration.

Caution! When the solution is heavy, it is better set the lowest

acceleration and deceleration.
2) Setting Parameter
Program No Rotor No Speed Temp Timer Acceleration Deceleration
1 30298 4200rpm 4℃ 1h20min 6 7

a Setting Program No
Press the button below P Window, after setting ―1‖, the digital tube flash
once.
b Setting Rotor No
Press the button below NO Window △ ▽，after setting 98 and the digital
tube flash once.
c Setting Speed
Press the button below SPEED Window △ ▽，after setting 4200 and the
 Jinan Biobase Biotech Co., Ltd.

digital tube flash once；Press ―RCF‖ button, it transfers to RCF

d Setting Time
Press the button below TIME Window △ ▽，after setting 1∶ 20，and the
digital tube flash once
e Setting Temperature
Press the button below TEMP Window △ ▽，after setting 04，and the digital
tube flash once
f Setting Acceleration
Press the button below A Window，after setting 6，and the digital tube flash
once
g Setting Deceleration
Press the button below D Window，after setting 7，and the digital tube flash
once
All the parameters are set, it shows as following chart 2

Chart 2

3.6 Start the centrifuge

Press the ―start‖ button, the green light of the ―start‖ button will be

brighten, the time starts to counts down.

Warning In the operation of the centrifuge, the operator

couldn’t lean on the centrifuge, and non-worker can’t stay
around the centrifuge.
 Jinan Biobase Biotech Co., Ltd.

3.7 Stop the centrifuge

It will cut off the electricity automatically in the process of operation when
the centrifugal time is 0, the red light will on , it will decelerating according
the parameters you set, when you hear the buzz sound, the rotors will
stop, the instrument also will stop running.

Part 4 Other Functions

1. Transferable showing of the RCF

The centrifuge is running according to the main parameter speed, if you want to
know the RCF in operation, press RCF button, the indicator light for RCF will
on, now the digits in the speed window is RCF value, press the RCF button
again , it shows the speed again.

2. Protection function
1). lid protection function
a. if the lid closing is not closing well, press ―start‖, the centrifuge cannot
run, speed screen shows E-1. Only close the lid properly, press ―start‖, the
centrifuge can run.
b. When the centrifuge is running, press ―open‖, the lid cannot open. This
program aims to protect the operator, not instrument error.
2）Imbalance Protection
If the test solution is imbalance, the centrifuge will stop automatically and
displays E-2.
3）Over speed protection
If the speed gets out of control and exceeds the maximum speed of the
 Jinan Biobase Biotech Co., Ltd.

rotor with 500rpm, it will stop automatically and you will see ―E-3‖ in the break
down window.
Warning：over speed set by the operator is forbidden！Which severely
break the operational rules and will result in accident, which cannot be
identified, so the duty of the accident is fully on the operator, In case of
accidents, the following operation is forbidden:
For example: the maximum speed is 3500rpm for NO.3 rotor, but the
operator mistakes it for NO.1 rotor, which maximum speed is 5000rpm, if the
operator set the speed at 5000rpm , it will working at the speed of 5000rpm,
then severely accidents will be happened.
4）Over temperature protection
Once the centrifuge reach the protection temperature, the centrifuge will
stop automatically, and displays E-4
5）Temperature measure protection
If the temperature measure is broken down, the centrifuge will stop
automatically and display E-5
6）Frequency changer protection
If the frequency changer is broken down, the centrifuge will stop
automatically and display E-6
7）Speed sensor protection
If the speed sensor is broken down, the centrifuge will stop automatically
and display E-7
8）Frequency changer communication protection
If the frequency changer communication is broken down, the centrifuge will
stop automatically and display E-0

3. Test sample protection

When the cover is closed or the machine cuts off suddenly, and you
want to take out the test tubes, then you can undo the screw which there is
 Jinan Biobase Biotech Co., Ltd.

mark in the side of the centrifuge by wrench, the cover will open，then take
out the test sample.

4. Except for the ―Rotors NO.‖ all the other parameters you can change in
the process of operation, it will work according the changed parameters.

5. Memory function
When all the parameters are set, they will be memorized by the machine.
The parameters in the window are what you set last time when you restart the
machine

Part 5 Trouble-shooting service

Trouble Shooting Table

Trouble Display Cause Solution

1 .No power supply Connect the power cord
E—1 2 .Lid is not closed Close the lid properly
Cannot Start
3.Frequency changer
E—6 Contact us
broken
1 The rotor is The weight difference of the
imbalance solution≤2g
Big vibration E—2 2 Centrifuge feet with
Adjust the force
imbalance force
3 Driving shaft bent Contact us
① micro control
Contact us
systerm
Over Speed E—3
② Speed sensor
Contact us
broken
Temp
E—4 Temp Measure broken Contact us
Measure
No display Switch broken Change switch
Cannot set Micro communication
E—0 Contact us
programs broken

Part 6 Other safety precautions

 Jinan Biobase Biotech Co., Ltd.

1. Forbidden for Artificial over speed

Danger Artificial over speed is a serious violation of operating
! procedures，easy to occur personal instrument
accidents.

2. The service life of the rotor ：the service life of the rotor is 5
years. The use of cumulative number is 3000 times, the accumulated
use time is 2000 hours. One of the three conditions is reached, the
duration of rotor is invalid. Prohibit to use the rotor with exceeding
service life.
3. Please check the rotors if had hair line before use every time. The rotors
will crack if using cracked and corrosion sign rotors.

Check rotor carefully before each operation.

! Danger Never use rotor with cracking, corrosion sign,

and never use expired rotor.

Warning When maintenance, please take off the plug,

wait at least 3 mins open the front cover, otherwise,
easy to occur electric shock.

4. Notice
Caution! The rotor head 、cup 、 bucket and adapter
needed for strict disinfection purification after separating
radioactive、toxic and viral material

Part 7 Maintenance
 Jinan Biobase Biotech Co., Ltd.

1. You should take out the rotors from the centrifugal chamber when you
don’t use the machine for a long time, and then store them in the ventilation
and dry place after cleaning.
2. Clean the centrifugal chamber when finished centrifugation,
dismantle the central sleeve from the axle regularly, and lubricate the axle
and central sleeve, in case of corrosion.
3. Keep the place which settle the centrifuge clean, make sure the freezer
is not choked by dirty subjects.

Part 8 Warranty Policy

1. We have one –year warranty on our all products

2. After the machine fixed, our professional technician will file it and
keep track of the usage of the machine.
3.We will pay attention to the feedback of the customers within 24 hours.
4. We won’t charge customers any money for repairing the machine in
one-year warranty time, the freight is also on us.
5. We only charge for the accessories on repairing the machine beyond
the one-year warranty time.
6. We have technician training class twice a year, training the technician
for our distributor.

Remark :

Regarding how to select RCF parameter

1. The principle of the centrifugation
Centrifuge will produce RCF during operation. Due to sedimentation caused
by RCF make the subject dangling in the solution to form precipitation. The
substance of the more proportion turned the direction of the largest radius
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rotor, the lighter substance is on heavier substance and let the subjects of
different proportion to be separated hierarchically.
2. How to calculate the relative centrifugal force (RCF)
Centrifugation is depending on the RCF, RCF is depending on the speed
and centrifugal radius, the formula of calculating the RCF as follows:

The transfer coefficient 11.2 is a approx value, which is calculating

according acceleration of gravity（1g = 9.81m/s2）
3. The confirmation of centrifugal time
Same RCF, centrifugation time is inversely proportional to centrifugal
solution’s proportion description. The more of the proportion, the less of the
time. The less of the proportion, the more of the time.
Same solution, centrifugation time is inversely proportional to RCF. The
bigger RCF, the less of the time. Contrary, the smaller RCF, the more of the
time.
Same RCF, centrifugation time is related to
Min centrifugal radius, longer basket(test
bottles) require a longer centrifugation time.
Therefore, the separation time is difficult to calculate. Usually it is decided
by the general test.