Multiple Choice

1. Which one of the following is a protein sequence database

A. DDBJ C. GenBank
B. PIR D. EMBL
2. All of the following are a protein sequence database except
A. SWISS PROT C. PSD
B. EMBL D. PIR
3. Which of the following is a sequence alignment tool
A. PIR C. BLAST
B. PRINT D. PROSITE
4. Which one of the following is the information retrieval tool of NCBI GenBank
A. Text search C. STAG
B. Seqln D. Enterz
5. Which one of the following database is important for the submission to GenBank?
A. Entrez C. Banklt and Sequin
B. Banklt and Bankln D. Sequin and Bankln
6. In the GeneBank the nucleic acid sequence database is maintained by
A. DDBJ C. NCBI
B. EMBL D. Brookhaven laboratory
7. Which one of the following is describe Flaybase
A. Model organism data base C. Biodiversity database
B. Biomolecular database D. Literature database
8. Which of the following alignment method is suitable for aligning closely related sequence?
A. Pairwise alignment C. Local alignment
B. Multiple alignment D. Global alignment
9. _______ is the alignment procedure that try to align the entire sequence
A. Pairwise alignment C. Local alignment
B. Multiple alignment D. Global alignment
10. _______ is the procedure used to align two sequences by searching for patterns that is in the
same order sequence.
A. Pairwise sequence alignment C. Sequence alignment
B. Multiple sequence alignment D. All of the above
11. Which one of the following alignment procedure is important to align many sequence
simultaneously?
A. Pairwise alignment C. Local alignment
B. Multiple alignment D. Global alignment
12. All of the following are sequence alignment tools Except
A. BLAST C. Clustal W
B. FASTA D. Rasmol
13. Which of the following is a multiple alignment sequence tool?
A. BPD C. Dismol
B. Clutal W D. Chime
14. Which one of the following is the advantage of sequence alignment?
A. Helps scientist to trace out evolutionary relationship
B. Helps scientist to predict the new members of gene families
C. Helps scientist to infer the functions of newly synthesized genes
D. All of the above
15. Which of the following alignment method is suitable to find out conserved pattern to
DNA/protein sequences?
A. Pairwise alignment C. Local alignment
B. Multiple alignment D. Global alignment
16. Which of the following is the first biological database?
A. DDBJ C. OMIM
B. GenBank D. Atlas of protein sequence and structure
17. Which of the following computer program is widely used in bioinformatics for multiple
sequence alignment with a command line interface
A. Primer expression C. CLUSTAL W
B. BLAST D. Target P
18. Among the following lists which are the nucleotide sequence database?
A. NCBI, PIR, MIPS C. EMBL, GenBank, NCBI
B. DDBJ, NCBI, PDB D. EMBL, Swiss Prot,PDB
19. Which one of the following tool is used for pairwise analysis of DNA sequence
A. BLAST C. MUSCLE
B. CLUSTAL W D. PROBCONS
20. Which of the following is open access database for public domain
A. NCBI C. EXSPASY
B. PDB D. All of the above
21. _____ is a database of current sequence map of the human genome
A. Golden Path C. GenCards
B. HGMD D. OMIM
22. Which one of the following is the advantage of BLAST program
A. DNA bar coding C. RNA sequence
B. Amino acid sequence D. DNA sequence
E.
23. Which one of the following is a protein database
A. GenBank C. PIR
B. EMBL D. DDBJ
24. Which one of the following is the advantage of BLAST program
A. To amplify DNA C. To design primers
B. To align sequence D. To find similarity between sequence
25. ______ is used to Clustal Omega program
A. Global alignment C. Multiple sequence alignment
B. Local alignment D. None of the above
26. Which of the following bioinformatics tool used for identification of Motifs?
A. BLAST C. PROSPECT
B. FASTA D. COPIA
27. Which of the following bioinformatics tools that can be used to characterize the Pan Genome?
A. NRCS C. FDA
B. PCR D. BPGA
28. Which of the following program is important to identify complete genome structure in genomic
DNA?
A. BLAST C. GENSCAN
B. PHYLIP D. SWISSPORT
29. Which one of the following software is not used in bioinformatics?
A. PROSITE C. AMPHORA
B. ORANGE D. EMBOSS
30. ______ is the data retrial system of NCBI
A. DBGET C. SAKURA
B. SRS D. Entrez
31. Which one of the following is not used to sequence alignment?
A. Draw Tree D. Pairwise alignment
B. BLAST E. None of the above
C. CLUTAL
32. Which one of the following is a model organism database?
A. PROMISE C. SCOP
B. GOLD D. SGD
33. Which one of the following is not BLAST programs?
A. BLASTN D. TBLASTN
B. BLASTP E. TBLASTX
C. BLASTX F. None of the above
34. During designing of the primers, to improve specificity of the primer it is necessary to avoid
A. Self-dimers C. Region of homology
B. Hetrodimers D. Region of cross homology
35. Which of the following is not the objective to perform sequence comparison?
A. To find the common motifs present in both sequences
B. To study the physical properties of molecules
C. To study evolutionary relationships
D. To observe patterns of conservation
36. Which of the following is untrue about SCOP?
A. It is constructed almost entirely based on manual examination of protein structures
B. The SCOP families consist of proteins having low sequence identity (>30%)
C. It is a database for comparing and classifying protein structures
D. The proteins are grouped into hierarchies of classes, folds, superfamilies, and families
37. Which of the following is incorrect regarding pair wise sequence alignment?
A. The most fundamental process in this type of comparison is sequence alignment
B. It is an important first step toward structural and functional analysis of newly
determined sequences
C. This is the process by which sequences are compared by searching for common
character patterns and establishing residue-residue correspondence among related
sequences
D. It is the process of aligning multiple sequences
38. Suppose if the two sequences share significant similarity, it is extremely ______ that the
extensive similarity between the two sequences has been acquired randomly, meaning that the
two sequences must have derived from a common evolutionary origin.
A. Unlikely C. Likely
B. Possible D. Relevant
39. Which of the following is incorrect regarding sequence homology?
A. Two sequences can homologous relationship even if have do not have common origin
B. It is an important concept in sequence analysis
C. When two sequences are descended from a common evolutionary origin, they are said
to have a homologous relationship
D. When two sequences are descended from a common evolutionary origin, they are said
to share homology.
40. Sequence similarity can be quantified using ________ homology is a ______ statement.
A. Percentages, quantitative C. Ratios, qualitative
B. Percentages, qualitative D. Ratios, quantitative
41. Which of the following is not correct about BLAST?
A. The BLAST web server has been designed in such away as to simplify the task of
program selection
B. The programs are organized based on the type of query sequences
C. The programs are organized based on the type of nucleotide sequences, or nucleotide
sequence to be translated
D. BLAST is not based on heuristic searching methods
42. Which of the following is incorrect?
A. Smith–Waterman algorithm is the fastest
B. Smith–Waterman algorithm is comparatively slower method
C. To speedup up comparison, heuristic methods are used
D. Heuristic algorithms perform faster searches
43. Which of the following is not a benefit or a factual of FASTA over BLAST?
A. FASTA scans smaller window sizes
B. It gives more sensitive results
C. It gives less sensitive results
D. It gives results with a better coverage rate for homologs
44. The initiation of FASTA format has ____ symbol.
A. > C. /
B. < D. *
45. FASTA and BLAST are __________ but __________ for larger datasets.
A. faster, more sensitive C. slower, less sensitive
B. faster, less sensitive D. slower, more sensitive
46. Which of the following is incorrect about Bootstrapping?
A. It is a statistical technique that tests the sampling errors of a phylogenetic tree
B. Tests by repeatedly sampling trees through slightly perturbed datasets
C. A newly constructed tree is not biased at all
D. The robustness of the original tree can be assessed here
47. Which of the following is incorrect regarding the advantages of Molecular data for
phylogenetics study?
A. They are more numerous than fossil records
B. They are easier to obtain as compared to fossil records
C. Sampling bias is involved
D. More clear-cut and robust phylogenetic trees can be constructed with the molecular data
48. Which of the following is incorrect regarding the terminologies of phylogenetics?
A. The connecting point where two adjacent branches join is called a node
B. Node represents an inferred ancestor of extant taxa
C. At the tips of the branches are long lost species or sequences
D. The lines in the tree are called branches