10.
This method detects in vitro sensitization
1. What is the color of the AHG reagent? of RBCs.
ANSWER: Green ANSWER: Indirect antiglobulin test
2. These are antibodies that target the human For numbers 11-18: Identify whether the
globulin or antibody. condition or application stated is used for or
detected through indirect antiglobulin test
ANSWER: Antihuman globulin (IAT) or direct antiglobulin test (DAT).
11. This method is applied in detecting
3. This antihuman globulin contains one antibody reacting with panel cells.
specificity, either anti-IgG or anti-C3d.
ANSWER: IAT DAT
ANSWER: Monoclonal AHG / Monospecific AHG
12. Antibody reacting with screening cells
4. In contrast to number 3, this contains both
anti-IgG anti-C3d. ANSWER: IAT
ANSWER: Polyclonal AHG / Polyspecific AHG
13. Hemolytic disease of the fetus and
newborn
5. This condition is associated with direct
antiglobulin test, wherein the recipient’s ANSWER: DAT
antibody coats donor’s RBC.
ANSWER: Hemolytic transfusion reaction 14. Antibody titration
(HTR)
ANSWER: IAT
6. This condition is associated with direct
antiglobulin test, wherein maternal antibody 15. Determination of RBC phenotype using
coats fetal RBC. unknown antisera
ANSWER: Hemolytic disease of the fetus and ANSWER: IAT
newborn (HDFN)
16. Confirmation of Rh-negative results
7. The patient has autoantibody which coats
his/her own RBC. ANSWER: IAT
ANSWER: Autoimmune hemolytic anemia
(AIHA) 17. Drug-induced hemolytic anemia
ANSWER: DAT
8. What is the specimen used in direct
antiglobulin test?
18. Detection of recipient’s antibody reacting
ANSWER: Patient’s RBC to donor cells.
ANSWER: IAT
9. What is the specimen used in indirect
antiglobulin test?
19. Polyclonal AHG is prepared using the
ANSWER: Patient’s serum conventional method of AHG production using
what animal?
ANSWER: Rabbit
20-22. Give at least 3 sources of error causing
false-positive reactions in antiglobulin test.
ANSWER:
(Reference: Harmening, D. M. (2019). Modern
Blood Banking & Transfusion Practices (7 th
(Reference: Harmening, D. M. (2019). Modern ed.). F. A. Davis Company, pg. 144.)
Blood Banking & Transfusion Practices (7 th
ed.). F. A. Davis Company, pg. 144.)
(Reference: Howard, P. R. (2017). Basic &
(Reference: Howard, P. R. (2017). Basic & Applied Concepts of Blood Banking and
Applied Concepts of Blood Banking and Transfusion Practices (4th ed.). Elsevier, pg.
Transfusion Practices (4th ed.). Elsevier, pg. 126.)
126.)
26. What will you do to the slide prior to Rh
23-25. Give at least 3 sources of error causing typing?
false-negative reactions in antiglobulin test.
ANSWER: Prewarm it on an Rh view box.
ANSWER:
27. To demonstrate agglutination in the
antihuman globulin test, what is the required
speed (rpm) of centrifugation and timeframe
in antiglobulin test?
ANSWER: 3400 rpm for 15 seconds
28. This serves as a negative Rh control. That
means that valid results of using this reagent
should have no agglutination.
ANSWER: 22% bovine serum albumin
29. How do you designate a certain individual
if he/she is tested negative in weak D testing?
ANSWER: Rh-negative weak D-negative
30. How do you designate a certain individual
tested positive in weak D testing but that
individual is a recipient?
ANSWER: Rh-negative
31-40. Illustrate the direct antiglobulin test
and give a brief discussion.
ANSWER:
41-50. Illustrate the indirect antiglobulin test
and give a brief discussion.
ANSWER:
