Pharmacokinetic exercise 1
Time (hr) Conc. (mcg/ml) Diff Time (Time Interval (Δt) Average Conc At each interval AUC
0 0
0.25 10 0.25 5 1.25
0.5 17 0.25 13.5 3.375
0.75 22 0.25 19.5 4.875
1 26 0.25 24 6
2 21 1 23.5 23.5
4 11 2 16 32
6 6 2 8.5 17
8 2.5 2 4.25 8.5
SUM ↑
1 Calculate AUC0-t AUC0-t 96.5
2 Calculate Kel Use Regression (Data Analysis)
conc log (conc) Time points
SUMMARY OUTPUT 21 3.044522438 2
11 2.397895273 4
Regression Statistics 6 1.791759469 6
Multiple R 0.996359036 2.5 0.916290732 8
R Square 0.992731329
Adjusted R Square 0.989096993
Standard Error 0.094582427
Observations 4
ANOVA
df SS MS F Significance F
Regression 1 2.443585848 2.443585848 273.1534519 0.003640964
Residual 2 0.017891671 0.008945836
Total 3 2.461477519
Coefficients Standard Error t Stat P-value Lower 95% Upper 95% Lower 95.0% Upper 95.0%
Intercept 3.785324708 0.115839342 32.67736702 0.000935182 3.286908245 4.2837412 3.2869082 4.283741171
Time points -0.349541546 0.021149274 -16.52735466 0.003640964 -0.440539526 -0.2585436 -0.4405395 -0.25854357
Kel= 0.3495 per Hour
3 Calculate T1/2
T1/2=Ln(2)/Kel
T1/2= 1.982832618
T1/2= 1.98 Hours
4 AUC₀₋∞= AUC0-t + extrapolated value
Extrapolated value= last observed conc. / (Kel from regression)
Extrapolated value= 7.153075823
AUC₀₋∞= 103.6530758
5 Cmax 26
6 T Max 1 hour
PK conc.-time graph
30
Cmax 25
AUC Btw Time points 2 and 4
20 hours = 1/2 × (a+b) × c
Conc. (mcg/ml)
15
10 a
AUC b
5
0
0 1 2 3 4 5 6 7 8 9
c Time in Hr
Tmax
