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Simple Bi

The document discusses molecular methods for pathogen detection, highlighting PCR assays that utilize specific primers for various genes, which are rapid, sensitive, and capable of differentiating species and biovars. Ideal samples for these assays include blood and tissues. Additionally, it mentions the FilmArray BioThreat panel, an automated multiplex PCR for detecting 17 human pathogens related to bioterrorism, including Brucella.

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ashish19216408ruby
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24 views1 page

Simple Bi

The document discusses molecular methods for pathogen detection, highlighting PCR assays that utilize specific primers for various genes, which are rapid, sensitive, and capable of differentiating species and biovars. Ideal samples for these assays include blood and tissues. Additionally, it mentions the FilmArray BioThreat panel, an automated multiplex PCR for detecting 17 human pathogens related to bioterrorism, including Brucella.

Uploaded by

ashish19216408ruby
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Molecular Methods :

❖PCR assay using primers for 16S- 23S rRna operon (rrs-rrl
gene), Omp2 gene (outer –membrane protein), insertion
sequence IS711, and protein BCSP31 are available
❑It is rapid , sensitive and specific and can also
differentiate between the species and biovars
❑Blood and tissues are ideal samples for PCR assays.

❖FilmArray BioThreat panel is an automated multiplex


PCR, useful for simultaneous detection 17 human
pathogens causing bioterrorism including Brucella.

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