Proimaging SARL
66 avenue des Champs-Élysées
75008 Paris, France
Phone: +33 (0)1 76 54 08 64
Email:
[email protected] Website: www.proimaging.fr
Product Specifications
LBL-Dye M715 – Far Red Mitochondrial Tracker
https://proimaging.fr/product/lbl-dye-m715/
Product Information
Product Name: LBL-Dye M715
Product Reference: BM298
Unit Size: 1mg
Shipping Conditions: Room temperature for up to 3 weeks
Storage Conditions: Store 18 months at -20°C and protect from light
Molecular Weight: ∼ 810
Max Absorption: 690 nm
Max Emission: 715 nm
Fluorescence Lifetime: 1.1 ns
STED Depletion Wavelength: 775 nm
Solubility:
- DMSO
- PBS Buffer (pH 7.4) + 1% polysorbate 80
Product Description
LBL-Dye M715 is a cell-permeant, lipophilic fluorescent dye with an emission wavelength of
715 nm, specifically designed for mitochondrial staining.
This photostable, non-cytotoxic probe is ideal for long-term imaging of living cells.
LBL-Dye M715 is compatible with various imaging techniques, including widefield
microscopy, confocal microscopy, high-content screening (HCS), flow cytometry,
fluorescence lifetime imaging (FLIM, with a lifetime of approximately 1 ns), and super-
resolution STED imaging, offering high depletion capacity. It can be used effectively on both
live cells and cells fixed with PFA post-labeling.
Product for Research Use Only. Not for Human Use.
LBL-Dye M715 – Far Red Mitochondrial Tracker 1/2
Proimaging SARL
66 avenue des Champs-Élysées
75008 Paris, France
Phone: +33 (0)1 76 54 08 64
Email:
[email protected] Website: www.proimaging.fr
Protocol of Use
Preparing Stock Solution
Solubility in DMSO / PBS Buffer (pH 7.4) + 1% polysorbate 80
To prepare a stock solution, dissolve the 1 mg lyophilized LBL-Dye M715 product in 125 µl of
high-quality, anhydrous dimethylsulfoxide (DMSO) to a final concentration of 8 mg/ml. The
solution must be aliquoted and stored at ≤–20°C and protected from light.
Experimental Protocols
Cell Preparation and Staining
The concentration of probe for optimal staining varies by application, cell type or on other
factors, such as the permeability of the cells or tissues to the probe. In general, use working
concentrations of 0,01% to 0,025% (0,8 to 2 µg/ml) – x4 000-10 000 from stock solution.
1. Preparation of staining solutions
Dilute 1 µl LBL-Dye M715 stock solution for 4 ml of appropriate buffer or growth medium.
2. Staining adherent cells
Cells grow on plate or coverslips with the appropriate culture medium. When cells have
reached the desired confluency, remove the media from the dish and add prewarmed (37°C)
staining solution containing LBL-Dye M715 probe. The probe can be applied directly in
culture medium without removal. While incubation times vary depending on the model
system and probe used, incubation for 15–45 minutes under growth conditions appropriate
for the cell type is generally sufficient but may need to be optimized. After staining is
complete, visualize the staining after exposure at optimal absorbance of 690 nm and detect
at 700-750 nm of emission wavelength. If background is observed, replace the staining
solution with fresh prewarmed media or buffer and observe cells using a fluorescence
microscope or fluorescence microplate reader. The cells can be fixed with PFA4% to preserve
the staining in fixed cells and tissues.
3. Staining suspension cells
Centrifuge with adapted speed to obtain a cell pellet and aspirate the supernatant.
Resuspend the cells gently in prewarmed culture medium (37°C) containing the LBL-Dye
M715 probe. While incubation times vary depending on cell type, incubation for 15–45
minutes under growth conditions is generally sufficient but may need to be optimized. After
staining is complete, observe directly or re-pellet the cells by centrifugation and resuspend
cells in fresh prewarmed medium if background signal is high.
Cells may be analyzed by flow cytometry, microplate-based analysis, or fluorescence
microscopy. The cells can be fixed with PFA4% to preserve the staining in fixed cells and
tissues.
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