MSC-B203 Series/MSC-B107 Series/MSC-B103 Series

MSC-B201 Series/MSC-B102 Series/MSC-B208 Series

BIOLOGICAL MICROSCOPE

Version 2022.11.01
 MSC-B102(Sliding)

Biological Microscopes are similar in appearance and operate in the same way;
MSC-B102 series Biological Microscopes are used as examples in the manual to
explain the operation steps.
 Content

I. Overview ................................................................................................. 1

II.Operation .................................................................................................2

III.Troubleshooting .....................................................................................4

IV. Symbols ................................................................................................ 6

V.Safety precautions ...................................................................................6

VI.Working environment ........................................................................... 8

VII.Maintenance and storage ..................................................................... 9

VIII.Unpacking ...........................................................................................9
 I. Overview
1.Description
Biological microscope is a special microscope used on biological and medical
treatment, apply to the microscopic measurement field, for health institutions,
laboratories, research institutes and institutions of higher learning units, such as
biological and bacteriological observation, education and professional studies. This
product adopts advanced design, models are novel, the variety of structure and
advanced technology, make the instrument easy , safe and reliable to use.
2.Structure

1 Fastening screws 11 Fine-tuning focal handwheel

2 Distance calibration loop 12 Move ruler
3 Diopter adjustment ring 13 Objective table lateral movement the handwheel (X)
4 Trinocular tube 14 Illuminator
5 Fixed screw for trinocular viewing head 15 Scale indicator
6 Revolving nosepiece 16 Move ruler
7 Objective 17 Stage
8 Move ruler 18 Gathering mirror fastening screw
9 Tighten the handwheel 19 Aperture diaphragm
10 Coarse focusing handwheel 20 Brightness adjustment handwheel
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II.Operation
1.Lighting
When open the power switch, the light bulb will be bright. Rotating brightness
knob to adjust the brightness of the field.
2.Adjust the interpupillary distance settings
Through the eyepiece, adjust the binocular barrel till around view is perfect.
3.Place specimen slides
Put glass slide on the stage, put the cover glass upward, part the specimen holder
shrapnel with fingers,and hold the glass slide.
4.Use 10X objective to focus
Rotate the converter, move the 10 x object lens to optical lattice (when rotated,
the converter will automatically locate object lens, focus the image on specimen by
turning the coarse and the fine focal handwheel. Selecting the appropriate
position,when the movement direction of the object stage and the focusing
handwheel is matched. Coarse fine-tuning hand wheel adjust the stage lifting, and
fine-tuning hand wheel is used to adjust the sharpness of the measured object
imaging.
5.Eyepiece diopter adjustment
According the left and right eyes’ diopter to adjust diopter adjustment ring.
Users can make full use of the advantages of the objectives and use the function of
parfocality.
1）Move the 40 x object lens to optical path, turn the coarse and fine focal
handwheel to focus the specimen.
2）Move the 10 x object lens (or 4 x object lens) to the optical path.
3）Left eye through the left eyepiece, by turning the left eyepiece apparent circle,
fine-tuning coke handwheel,to focus on specimen. Repeat the above steps to see
images by left and right eyes at the same time.
6、Condenser vertical position adjustment
Using the condenser lifting handle to transfer it to the upper limit, then lower

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it down a little bit. If you find scattering field background images, you can adjust the
condenser slightly up and down,to make it disappeared.
7.Selection of the objectives
Rotate lens converter, select the desired objective magnification, adjust the
according to the selected object lens.
8.Aperture diaphragm adjustment
Put the regulating rod dial of condenser aperture diaphragm to the optical path to
make the ratio of the objective same number.
Aperture diaphragm adjustment
① The regulating rod dial of aperture diaphragm changes its scale. If a narrow
aperture, brightness and resolution will be lower, and the contrast and depth of field
will increase. On the contrary, if a large aperture, brightness and resolution will be
increased, while the contrast and the depth of field will be reduced.
② In order to obtain ideal and appropriate contrast image, you can make the aperture
diaphragm adjustment transferred to the numerical aperture of the objective of 70%
to 80%. Aperture diaphragm controls the numerical aperture of the condenser. Don't
use it to adjust the brightness,you can use brightness control knob to adjust the
brightness instead.When observing the aperture diaphragm,please move the eyepiece,
use the binocular tube to observe directly.
③ Numerical aperture of the condenser is said in the corresponding objective
multiple,40 x / 0.65 tag is refers to the magnification of 40 x, numerical aperture of
0.65, the position of the aperture adjustment lever.
9.Oiled observation
Labeled "oil" font object lens for oil immersion objective. When using the oil
objectives,they should be oiled by special microscope liquid between the lens and
cover glass. Make sure that the cap is tight after using the oil.The cap may be a little
loose after long-term use.So you should inspect the cap regularly to protect the oil
from spilling. Don't extrude container excessively, it may be the cause of oil
overflowing suddenly from the container. In the using process,please wipe the oil

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on the container.
10.Adjust the torque of coarse focusing handwheel
The coarse focusing handwheel looseness is adjustable.If you want to increase
the torque, you can twist the torque adjustable ring of the coarse focusing handwheel
counterclockwise.On the contrary,twist it clockwise. Don't tune it too loose, or the
stage will be dropped automatically.

III.Troubleshooting
Problem Causes Remedy
Power cord of the power supply Plug in the power cord into a
unit is unplugged. power outlet.
1) The bulb Main switch of the power supply Set the main switch to “I”
does not light. unit is not ON. (ON).
The fuse is burnt out Replace the fuse.
The bulb is burnt out. Replace the bulb.
Increase light intensity to an
The voltage is too low
optimum voltage.
Adjust the condenser height
until the field iris diaphragm
Condenser is not well positioned.
image is formed in the
specimen plane.
2) The bulb Center the condenser so that the
lights but the Condenser is not centered. field iris diaphragm image is
field of view is centered in the field of view.
dark. Make sure that the revolving
Revolving nosepiece is not in a
nosepiece clicks properly into
click position.
place.
Field iris diaphragm is not Open the field iris diaphragm
opened wide enough. sufficiently.
Reduce the number of filters to
Too many filters are used.
the minimum required.
The objective that falls outside
Use a condenser that matches
the condenser’s illumination
the objective.
range is used.
3) Field of
Field iris diaphragm is not Center the field iris diaphragm
view is
properly centered. correctly.
obscured or not
Field iris diaphragm is stopped Open the field iris diaphragm
evenly
down too far. sufficiently.
illuminated.
Revolving nosepiece is in an Engage the revolving nosepiece
intermediate position at a click stop.
A filter is stopped in an Set the filter at the appropriate

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 intermediate position. position.
The frost filter is not engaged. Engage the frost filter.
Dirt/dust on the specimen.
Dirt/dust on the eyepieces.
Clean thoroughly.
Dirt/dust on a mirror unit.
4) Dirt or dust
Dirt/dust on the optical element.
is visible in the
Adjust the condenser height
field of view. Condenser is not correctly
until the field iris diaphragm
positioned and the frosted filter
image is formed in the
or filter is focused.
specimen plane.
Condenser is raised too high. Lower to the proper position.
5)Image glares Aperture iris diaphragm is Open the aperture iris
stopped down too far. diaphragm.
Objective in use is not designed Replace with an objective
for UCIS series. designed for UCIS optics.
Front lens of the objective is
6)Visibility of Clean the objective.
dirty
observe image
Immersion oil is not being used
is poor, Image Use immersion oil.
with an oil immersion objective.
is not sharp
Inappropriate slide or cover glass Replace with glass of
Contrast is
thickness. appropriate thickness.
poor,Details are
Dirt/dust on glass components
poorly visible.
(condenser, objective, Clean thoroughly.
eyepieces, )
Phase plate are not centered. Center it.
Make sure that revolving
Objective is engaged incorrectly
nosepiece clicks intoplace
7) Image is in the light path.
correctly.
blurred.
Specimen is tilted with respect to Place the specimen correctly on
the stage. the stage.
The interpupillary distance is Adjust the interpupillary
incorrect. distance.
Incorrect diopter adjustment. Adjust the diopter.
8) Field of When looking into eyepieces,
view of one eye do not stare at image from the
does not match beginning but see the overall
that of the You are not accustomed to field of view. It is sometimes
other. parallel optical axis. recommended to turn your eyes
away from the eyepieces, look
far off and look in to the
eyepieces again.
9) The The rotation tension adjustment
Loosen the ring optimally.
coarse/fine ring is too tight.

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adjustment
knobs will not
rotate easily or
at all.
10) The stage
The rotation tension adjustment
lowers by its Tighten the ring optimally.
ring is too loose.
own weight.

IV. Symbols
1.Safety symbols on microscope
The following symbols are found on the microscope. Study the meaning of the
symbols and always use the equipment in the safest possible manner.

2.Caution Symbols in this Manual

If the microscope is used in a manner not specified by this manual, the safety of
the user may be imperiled. In addition, the equipment may also be damaged. Always
use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.

V.Safety precautions
5.1 After the equipment has been used in an observation of a specimen that is
accompanied with a potential of infection, clean the parts coming in contact with the
specimen to prevent infection.
Moving this product is accompanied with the risk of dropping the specimen. Be
sure to remove the specimen before moving this product.
In case the specimen is damaged by erroneous, promptly take the infection

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prevention measures.
5.2 The microscope is provided with a simplified waterproof mechanism. Therefore,
if culture liquid or water is split on the stage, revolving nosepiece or microscope
frame, damage to the equipment or an electrical shock may result. Immediately wipe
the liquid or water off if it is spilt on them.
5.3 The microscope is not covered by warranty in terms of laser safety. The user
should assume liabilities for any consequence of user modification,
5.4 The surfaces of the lamp housing will become extremely hot during long-time
operation. Be sure to keep the flammable stuffs such as paper, alcohol, oil away from
the lamp house to avoider.
5.5 When using the microscope, route the power cord away from the lamp housing.
Should the power cord come in contact with the hot lamp housing, the power cord
could melt and cause electric shock.
5.6 To avoid potential shock hazards and burns when replacing the light bulb, set the
main switch to “O” (OFF) then disconnect the power cord from the wall outlet in
advance. Whenever you replace the bulb during use or right after use, allow the lamp
housing and bulb to cool before touching.
5.7 Electric shock warning:
Remove of the bottom cover of the microscope makes the dangerous electric
parts inside exposed. Any contact with these parts may cause shock or death. In event
of maintenance, please apply to qualified professionals for help.
5.8 The G4 bulb socket is designed specially for 6V/20W halogen bulb. Damage will
occur if bulb of different description is replaced.
5.9 Always be sure the power cord provided by the supplier. If the proper power cord
is not used, product safety performance cannot be warranted.
5.10 To avoid potential shock hazards when replacing the fuse, set the main switch to
“O”(OFF) then disconnect the power cord from the wall outlet in advance.
5.11 Always ensure that the grounding terminal of the microscope and that of the wall
outlet are properly connected. If the equipment is not grounded, the supplier can no

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longer warrant the electrical safety performance of the equipment.
5.12 Never insert metallic objects into the air vents (A⑨ in Fig.2) of the microscope
frame as this could result in electrical shock, personal injury and equipment damage.
5.13 A microscope is a precision instrument. Handle it with care and avoid subjecting
it to sudden or severe impact.
5.14 Moving the Microscope
When moving the microscope, remove the observation tube, then carefully carry
the microscope frame by the base (front edge)(② in Fig.1) and the grasping part(①in
Fig.1) in the upper rear.
Also be sure to remove the specimen since it may fall.
When moving the microscope for a long distance, it is also recommended to
disconnect all cables from the equipment.
When transporting it, also engage the adhesive tape lock mechanisms and
package it sufficiently.
Also be careful against slipping of hands during carrying.
★ Damage to the microscope will occur if you grasp it by other parts including the
stage, coarse/fine adjustment knobs, the nosepieces, etc.

VI.Working environment
 Indoor use.
 Ambient Temperature: 5℃to 40℃ (41℉to 104℉)
 Max.relative humidity: 80% for temperatures up to 31℃ (93℉), 60% at
37℃(99℉), to 50% relative humidity at 40℃ (104℉).
 Supply voltage fluctuation: ±10%
 Pollution degree: 2 (In accordance with IEC60664)
Electric power specification
 Input: 100-240V～0.5A, 47-63Hz
 Output: 6V 3.4A
 Fuse: 3.15A, 250V, F ф5×20mm
 Halogen Bulb Socket: G4

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VII.Maintenance and storage
7.1 Clean all glass components by wiping gently with gauze. To remove fingerprints
of oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%)
and alcohol (30%).
Since solvents such as ether and alcohol are highly flammable, they must be handled
carefully. Be sure to keep these chemicals always from open lames, or potential
sources of electrical sparks—for example, electrical equipment that is being switched
on or off. Also remember to always use these chemicals only in a well-ventilated
room.
7.2 Be sure to clean the oil immersion objective after use. Leaving immersion oil on
it will degrade its performance.
7.3 Do not attempt to use organic solvents to clean the non-optical components of
microscope. To clean them, use a lint-free, soft cloth slightly moistened with a diluted
neutral detergent.
7.4 Never attempt to disassemble any part of the microscope.
7.5 When not using the microscope, make sure to set the main switch to “O” (OFF),
conirm that the lamp housing is cool enough and cover the microscope with the
provided dust cover.
7.6 Do not use the microscope where it is subjected to direct sunlight, high
temperature and humidity, dust or vibrations.

VIII.Unpacking
8.1 Please check all the components according to the packing list in the package as
you unpacking the microscope. Contact us or our distributor as soon as possible if
any component is missed in the package.
8.2 Before transporting the microscope, we have fixed the flexible parts of the stage
with pieces of adhesive tape, please remove the adhesive tapes before use.

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