1AND2 1996 Reff2022-1
1AND2 1996 Reff2022-1
(Reaffirmed 2012)
(Reaffirmed 2017) Reaffirmed 2008
IS 14399 (Parts 1 and 2) : 1996
(Reaffirmed 2010)
(Reaffirmed 2009)
Indian Standard
(Reaffirmed 2008)
HOT PRESS MOULDED THERMOSETTING
GLASS FIBRE REINFORCED POLYESTER
RESIN (GRP) SECTIONAL WATER
(Reaffirmed 2007)
STORAGE TANKS
(Reaffirmed 2006)
ICS 23.020.10
(Reaffirmed 2005)
© BIS 1996
(CEO 3)
ReproaraPhY Unit.SIS. New Del~ India
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FOREWORD
This Indian Standard was adopted by the Bureau of Indian Standards, after the draft finalized by the
Sanitary Appliances and Water Fittings Sectional Committee had been approved by the CivilEngineering
Division Council.
This Indian Standard has been prepared with a view to provide guidance in the manufacture of glass fibre
reinforced polyester resin sectional tanks for water storage made with hot compression moulded panels.
Part 1 of the standard covers panels for the tanks made by hot compression moulding process and Part 2
covers assembly, installation and testing procedure for these tanks.
In the formulation of this standard, assistance has been sought from Singapore Standard SS 245 : 1981
'Specification for glass reinforced polyester sectional water tanks'.
The composition of technical committee responsible for the formulation of this standard is given at
Annex L.
For the purpose of deciding whether a particular requirement of this standard is complied with, the final
value, observed or calculated, expressing the result of a test or analysis,shall be rounded off in accordance
with IS 2 : 1960 'Rules for rounding off numerical values (revised)'. The number of significant places
retained in the rounded off value should be the same as that of the specified value in this standard,
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Indian Standard
HOT PRESS MOULDED THERMOSETfING
GLASS FIBRE REI-NFORCED POLYESTER
RESIN (GRP) SECTIONAL WATER
STORAGE TANKS
PART 1 SPECIFICATION FOR PANELS
1
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3.12.3 Half and Quarter Panels shall be weighed and boiled for further 200 hand
re-weighed. There shall be no evidence of weight
Inlets, outlets and drains can be combinations of
loss (due to break down of the polymer).
smaller panels namely half and quarter panels or
full panels. The assembled outer dimensions of 4.3 Glass Fibre Reinforcements
these panels shall be as prescribed in 5 of Part 2.
Glass fibre reinforcement shall be of commercial
3.12.4 Drain Panel Assembly grade E type and shall conform to IS 11273 : 1992,
Combination of half, quarter or full panels con- IS 11320: 1985 or IS 11551 : 1986 as appropriate.
toured to allow complete draining of water and 4.4 Fillers
having required flat surface of even thickness to
mount drain fittings. Inert inorganic fillers (with particle size below
3.12.5 Manhole Panels
0.05 mm) shall only be used, if required.
The panelswhichprovideaccess to the insideof the 4.5 Additives
tank for cleaning/inspectionpurpose. Additives may be incorporated for modifying the
4 MATERIALS properties of the resin.
4.1 The material to be used shall be composed of S COLOUR
unsaturated thermosetting polyester resin (food
grade) reinforced with glassfibre. This systemwill Colour of the panel shall be a shade of grey or
include catalysts and may include pigments (com- cream. Any other colour (pastal shade) may be
patible with unsaturated polyester resin) and ultra used,as mutuallyagreed betweenthe purchaser and
violet stabilizers. These materials coming in con- the manufacturer.
tact or likely to come in contact with water should 6 I)ANEI.I DIMENSIONS
not constitute toxic hazard, should not support 6.1 Flange of I)n~lels
microbial growth and should not give rise to un-
pleasant taste or odour, cloudiness or discoloura- The panels shall be manufactured with a combined
tion of water. Tank manufacturer should ensure double flangeat an angle of 45° and 90° to face of
and obtain a certificate to this effect from the raw the panels on all four sides or a single flange at an
material manufacturer. angle of 900 to the faceof the panels on each of the
4.2 Polyester Resin 2, 3 or 4 sides. The flange shall be provided with
holes to accommodate fasteners according to the
Appropriate lype of unsaturated polyester resin position of the panels in the tank and they should
systemconforming to IS 6746 : 1994 shall be used. be free from irregularities.
4.2.1 The resin shall meet the following charac- 6.1.1 The joint width (land width) of the flanges
teristics when tested as per IS 6746 : 1994: shall not be less than 45 mm (see Fig. 1).
a) Specific gravity = 1.13 ±.Ol at 27°C, 6.2 The nominal external size of the unit panels
b) Acid value = 16±4 mg kol-l/g, shall be 1 meter square or 1 m x 0.5 m or 0.5 m x
c) Volatile content = 30 percent ± 3 percent, 0.5 m or any other size as agreed to between the
and purchaser and the manufacturer.
d) Gel time at 25°C = 20 to 30 minutes 6.3 Tolerance in the external dimensions of each
4.2.2 The following characteristics of the cured panel shall be within ± 0.2 percent of the external
resin shall also be met with, when tested in accord- dimensions. Tolerance on the angles shall be
ance with IS 6746 : 1994 : within ± 0.3°.
a) Barcol hardness = 40 BHU Min; 6.4 Type/Thickness of Panel
b) Heat deflection The type and corresponding minimum thickness of
temperature = SOOC to gooC the panels shall be as follow:
c) Elongation at = 1.9 percent, -0, Type of Panel Min Thickness, mm
break +25 percent, and
A 3
d) Water absorption = 1 percent,
after 7 days at 25°C Max. B 4
4.2.2.1 Hydrolysis test C 5
The cured resin samples of size 50 mm x 50 rom NOTE- Halfand quarter panel inType A, BorCshall have
the correspondingminimumthickness as specified aboveand
x 3 mm approx shall be immersed and boiled in these shall meet the requirements stipulated in 6.1, 6.1.1, 6.2
distilled water at lOOOC. After 200 h resin samples and 6.3.
2
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o PITCH
-CORNER JOINT
o~ o}
---~INTERMEDIATE JOINT
1A
"'"
::t:
9
~
PITCH
o o
~ CORNER JOI NT
d
~ rr"I~===~
~ _ _ ~_A.l.-_ _---J . _ 0 Ci~ 0 )~--U
LINTERMEDIATE JOINT
1B
o 6 0 I
HOT DIP GAlVANISED MSOR 1C
SMC CORNER PIECE
from panels or Irom flat laminates prepared in the determine the degree of effect on taste, odour,
same composition and by the same techniques as colour and turbidity, the cha_nge in levels of toxic
the panels. metals and other toxic substances and whether or
not the panel supports the growth otbactcrla, algae
8.2 The test specimen shall be prepared and tested
and fungi. These effects shall be within the limits.
for the tests specified in Table 1 in accordance to
the methods described in relevant Annexes for the 11.2 EfTectonTaste, Odour, Colour and 'furbldity
mechanical/physical properties and shan meet the
specified acceptance criteria. When tested by the method described in Annex J
the 7th extract shall show no deterioration of
Table 1 Mechanical/Physlcal Properties, COIOUf or turbidity as compared with the original
Acceptance Criteria and Test Methods tap water.
Taste and odour should be assessed on the 7th
81 Physicol Property Acceptance Test Method extract or on earlier extracts if all taste and odour
No. Criteria
has gone. This extract shall not have a taste or
i) Tensile strengt h 70 MPa (Mill) Annex B
odour described as 'chlor-phenolic', 'medicinal', or
ii) Bending strength 100 MPa (Min) Annex C
'disinfectant'. Other tastes may be present in this
(cross-breaking)
extract if theyare not detectable at dilutions greater
iii) Elastic modulus in 6 ()()() MPa (Mi,,) AnnexD
bend than 1 + 3 with tap water (that is, a thresholc.J odour
lv) Glass content 25 percent (Mill) AnncxE
number of 4). Tastes described as 'rubber',
V) Barcoal hardness 50 BI-IU (Mill) AnncxF
'plastics', 'greasy', 'waxy' or similar dcscripi ions
may be present in this extract if they arc not detec-
vi) Water absorption 0.5 percent (Mat:) Annex G
table at dilution greater than 1 + 6 with tap water
9 IIYDROSTAl"IC TES1~ (that is, a thresh?ld odour number of 7).
Panels of Type A, Band C when fixed to a hydros- 11.3 Toxic Metals
tatic tester as described in Annex H, shall withstand
the following hydrostatic pressure without burst- Toxic metals should be assessed on the extract or
ing, cracking or leakage: on earlier extracts if these are within the recom-
TypeofPanel Hydrostatic Pressure, MPa mended limitswhen tested bythe method described
in Annex J, the amounts of toxic metals in this
A 0.04 extract shall not exceed the limits specified in
B 0.08 IS 10500 : 1991.
C 0.12
11.4 Cytotoxicity
10 DE.FLECfION'rEST
When tested by the method described in Annex J,
10.1 This test shall be carried out simultaneously the extract after the third extraction, if necessary,
with the hydrostatic test and the maximum deflec- shall be free from cytotoxicity, lethe control system
tion in the panel shall not exceed 10 rom. failsto support thecontluent growth of the cell line,
10.1.1 The deflections shall be measured at the the results of the test shall be regarded as invalid.
centre of the panels using a dial gauge graduated to If confluent growth of the cells docs not occur in
0.01 rom having a range of at least 25 mm. contact with the third extract of the test material,
this should be interpreted as a failure in this lest.
10.1.2 The gauges shall be first set to zero and then
the panel pressurised. 11.5 Microbiological Growth
10.1.3 The deflection reading shall be taken imme- When tested by the method described in Annex J,
diately after the working pressure is reached. the counts of coliform organisms and Pseudomonas
10.1.4 Working pressure shall be maintained for aeruginosamade4 weeks,Sweeks and 6 weeks after
4 h and the deflection at the same position shall the initial inoculation shall be less than 1 per 100
again be taken and the greater of the two results ml. All the counts of bacteria capable of growth at
used. 37°Cand 22°Cand fungi made 4 weeks,5 weeks and
6 weeks after the initial inoculation shall be less
11 TES'f ES'fABLISIIING PO'fABILITY OF than 10 times the corresponding count in the con-
WATER (fYPE l·ES'f) trol flask.
11.1 General Irrespective of the above requirements however
The fibreglass panels for water tanks shall not con- counts shall be deemed to be acceptable if they are
taminate the water. Tests shall be included to less than the. maximum limits given in Table 2.
4
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ANNEX A
(ClaLLSe 2)
LIS1' OF REFERRED INDIAN STANDARDS
5
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ANNEX B
(Clause 8.2)
TENSILE STRENGTH
n-i APPARATUS The four end pieces of anyone test specimen shall
be of the same thickness, which shall be not less
A testing machine that can be operated at constant
than 3 mm, They shall be prepared from material
rates of crosshead motion.
similar to that under test.
B-2 TEST SPECIMEN NOTES
Five specimens shall be used for this test. 1 Before the adhesive is applied the surfaces to be bonded
should be slightly roughened with a fine grade of abrasive
The test specimen shall be a rectangular strip not paper and thoroughly cleaned with solvent. It is recom-
less than 230 mm long and 25 ± 0.5 mm wide with mended that the assembly should be allowed to stand over-
night while under pressure.
four rectangular end pieces, as shown in Fig. 2,
2 Test specimens can be sawnfrom the blank (see Fig.3 ) by
bonded to it with a cold-hardening epoxide resin. means of a carbide-tipped fine toothed circular saw or a
The thickness of the rectangularstrip shall be that nylon-bonded carborundum wheel. The sawing operation
of the sheet under test. The faces shall be parallel must not overheat the specimen and the sawnedges must be
to within 0.15 mm. free from imperfections. It is essential that the edges of the
specimen arc not llnished by hand.
I~----.--------.....---~
1.5 min. 11.0 ±5 I 4S min.
1
T
25 :to·S
.l..
230 min .
...
All dimensions in millimetres.
FIG. 2 TEST SPECIMEN
6
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ANNEX C
(Clause 8.2)
BENDING STI{ENGTII
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ANNEX D
(Clause 8.2)
ELASTIC MODULUS IN BEND
ANNEX E
( Clause 8.2 )
GlASS CONTENT OF GLASS-REINFORCED LAMINATES
E-l AI)PARArrUS than 400 mm2 and whose shorter edges are not less
than 12 rom 'long,is usuallysuitable.
a) A weighingbalance with an accuracyof ± 10
mg, E-3 PROCEDURE
b) A muffle furnace,
c) A crucible of appropriate dimensions, and A crucible of appropriate dimensions shall be
d) A desiccator. heated in a muffle furnace at 575°C±25°C for 15
minutes, cooled in a dessicator and weighed (WI).
E-2 rrEST SPECIMEN
The lest specimens shall be placed in the crucible
Two specimensshall be tested for sample contain.. and the crucible and contents heated in an oven at
ing no filler and colouring agent according to 105°C to 1100C for 2 h. The crucible and contents
E-3(a). Three specimensshall be tested for sample shall then be cooled in a dessicator, weighed and
containing filler and/or colouring agent according returned to the oven for a further 30 min, cooled
to E- 3(b). and reweighed. This procedure shall be repeated
until the mass (W2) of crucible and specimen
The specimen may be of any convenient shape becomes constant to within 0.01 g:
provided that it is representative of the material
under test and that its massis not less than 5 g. A a) Sample containing no filler and colouring
rectangular specimen, with a face of area not less agent:
8
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The crucible and contents shall then be l100C for 3 h or more until the mass of the
heated in a ventilated muffle furnace at a glass residue (W4) becomes constant to
temperature of 575°C±25°C for 30 minutes within 0.01 g.
or more, until the residue of glassfibre is
E-4 CALCULATION
white in colour. The crucible and contents
shall then be removed from the furnace, E-4.1 For samples determined according to E-3(a):
allowed to cool in a dessicator and weighed. 100 (JV3 - Wl)
This process of heating, cooling and weigh- Percentage glasscontent = (W2 _ Wi)
ing shall then be repeated until the mass where
(W3) becomes constant to within 0.01 g. Wt = mass of crucible,
b) Sample containing filler and/or colouring W2 = mass of sample + crucible, and
agent: W3 = mass of glass residue + crucible.
The crucible and contents shall be heated in E-4.2 For samples determined according to E-3(b):
a ventilated muffle furnace at a temperature lOOW4
Percentage glass content = W2 _ lVl
of 575°C± 25°C to burn out the resin com-
pletely (by repeating the process of heating where
and weighing as above), leaving behind the WI = mass of crucible,
glass, filler and colouring agent. Water shall W2 = mass of sample + crucible, and
then be added to the residue and the mixture W4 = mass of glass residue.
shall be filtered through a tared standard
sieve of 200 mesh size. Wash the residue The glass content of the material under test shall be
with water till the filtrate is clear. The glass reported as the arithmetic mean of the results
residue shall then be heated at 105°C to obtained on the test specimens.
ANNEX F
(.Clause 8.2 ~
BA1{COL lIAltUNl.:sS
."·1 AI)PARATUS catcd cone having an angle of 26° with a flat tip of
0.157 mm in diameter. It shall fit into a hollow
F-l.l Indentor
spindle and be held down by a spring loaded
The indentor shall consist of a hardened steel trun- plunger (see Fig. 4).
COVER
SCREW---- PIN
LEVER --+__--S!.IIJj~~.......~
LOCK
LEG
POINT SLEEVE
STOP SPRING LOWER PLUNGER
POINT SPRING GUIDE
9
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.'·1.2 Indicating Device Set the point sleeve on the surface to be tested. Set
the legson the same surface or on solid material of
The indicating dial shall have 100 divisions, each the same thickness,.50 that the indentor is perpen-
representing a depth of 0.007 6 mm penetration. dicular to the surface being tested. Grasp the in-
F-2 TEST SPECIl\fENS strument firmly between the legs and pointsleeve.
Apply quickly, by hand, uniformlyincreasing force
Number of test specimen shall be taken as five. on the case until the dial indication reaches a
The testing area shall be smooth and free from maximum. Record the maximum readingobtained.
mechanicaldamage.
F·4 CONDrrIONING
Test specimens shall be at least 1.5 mm thick and Test specimensshall be conditioned at 27°C ± 2°C
large enough to ensure a minimum distance of 3
and 6S ± 5 percent relative humidity for at least
mm in any direction from the indentor point to the
40 h prior to test.
edge of the specimen.
14'-3 PROCEDURE F ~s NUl\fBER OF READINGS
The test shall be carried out at a temperature of Minimum number of readings per sample shall be
27°C ± 2°e and 65 ± 5 percent relative humidity. eight, taking care that impressions arc not made
within 3 mm of the edge of specimen or earlier
Place the impressor and the material to be tested impressions.
(for the calibration disc) on a solidly supported,
fiat, hard, firm surface such as stone, metal or F-6 REPORT
ceramic. If softer supporting surfaces are used, a
falsely low instrument reading mayoccur. Report the average of the hardness value.
ANNEX G
( Clause 8.2 )
WATER ABSORPTION
c-i APPARATUS contact over any substantial area with one another
or with the container. The specimen shall be dried
A weighing balance with an accuracy of ± 10 mg.
with a cleancloth or filter paper and, not more than
G-2 'fEST SPECIMEN 2 min after removal from the water, shall be
reweighed (W2).
Two specimens shall be used for this test.
The specimen shall be 38±O.5 mm2 and shall have G-4 CALCULATION
smooth edges. The absorption coefficient shall be determined
The thicknessof the specimenshall be the thickness from the following:
of the sheet under test. A = W2 - WI X 100
Wt
The surfaces of all specimens shall be free from where
dust, oil or other matter that might interfere with A = absorption coefficient (percent);
the absorption of water. W2 = massof specimen after immersion in
G-3 })ROCEDURE water, mg; and
Wt = mass of specimen before immersion
The thickness of the test specimen shall be in water, mg.
measured to the nearest 0.025 mm. The specimen
shall then be weighed (WI) and immersed for 24 The water absorption of the material under test
± 1 h in distilledwater at 27°C ± 2°C. Precautions shall be reported as the average of the water ab-
shall be taken to prevent specimens from making sorption of the test specimens.
10 -
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ANNEX H
(Clause 9)
I1YDROSTATIC TEST
11-1 EQUIPMENT
11-1.1 A hydrostatic tester with a minimum pressure
of 200 kPaforthe panel (see Fig. 5).
PRESSURE
GAUGE
WATER PRESSURE
ANNEX J
(Clauses 11.2, 11.3, 11.4altd 11.5)
ESTABLISIIING P01·ABILITY OF WATER
11. '
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to be used in succession for each of the remaining should preferably be made by atomic absorption
3 tests. spectrophotometry, or by any other standard
J-2.2 Size of Specimens
methods as agreed by the purchaer or inspection
agency.
The surface area of eachspecimen shallbe 150cm2
± 10 percent. The specimen should not exceed 15 J-4.3 Repeat Tests
emin lengthand 6 emin width or depth. For sample If the results obtained are greater than the limits
containers the capacity should be between 1 to 5 specified in IS 10500 . : 1991 for drinking water,
litres. make further 24 h extractions until results lower
J-2.3 Sample Preparation than these limits are obtained up to a maximum of
7 dailyextractions.
Specimens of coatings shall be applied to water
tank or containers of an appropriate materialof the J-S METIIOD OF TEST FOR CYfOTOXICIlY
size described above, including all appropriate J-S.l Extraction
primings and under coats. and completelycovering
all sides and edges. Specimens specially prepared Immerse the test specimen in 750 ml of tap water
for testing shall be subject to the same conditions contained in a borosilicate glass container for a
of maturing or curlng as would normally be period of 24 h at room temperature. In the case of
achieved either in manufacture or on site. containers, use at least one litre of water, Store an
Specimens shall not be SUbject to any specialized additional quantityof tap water for the same period
cleaningprocedures. except where this would nor- and under the same conditions as control. Cover
mallyoccur. the containers during period of extraction.
J-3 METHOD OF TEST FOR TASTE, ODOUR, J-S.2 Materials and Media
COLOUR AND TURBIDIlY
J-S.2.1 CellLine
J-3.1 Extraction African green monkey kidney cells (VERO estab-
Immersethe test specimensin 750mlof odour-free lished cell line ATCC Number CCL 81) or
lap water contained in glassvessels for a period of equivalent shall be used.
24 h, 48 hand 7 daysat room temperature. In the J-S.2.2 Media
case of containers, place at least one litre of water
in the sample. Store an additional quantity of Commercially available media may be used
odour-free tap water for the same periods of time wherever practical provided that they are knownto
and under the same conditions as control. be suitable for use with the cell line. Only glass
distilled water, known to be free from any cytotoxic
J-3.2 Assessment
effects shall be used.
The extractsshall be examinedat theend of24 and
48 hand 7 days for taste. odour, colour and J-5.2.2.1 Growth medium
turbidity and it shall satisfy the requirements of
11.2. Distilled Water • 90 ml
199 concentrate (XIO) with Earle's 10 ml
J-4 METIIOD OF TEST FOR TOXIC METALS salts but without sodium bicarbonate buffer
J-4.1 Extraction Newborncalfserum 7 ml
Gentamicin solution (4000 i.u.ml- 1) 1 ml
Immerse the test specimen in 750 ml of distilled 4.4 percent sodium bicarbonate buffer 2 ml
water contained in aglasscontalner for a period of saturated with carbon dioxide
24 h. In the caseof containers. use at least one litre
of water. Store an additional quantity of distilled J-5.2.2.2 Maintenance medium
water for the same period and under the same
conditions as control. Distilled Water • 90 mt
199concentrate (XIO) with Earle's 10 ml
J -4.2 Assessment salts but without sodium bicarbonate buffer
Determine the quantities of toxic metals in the. Newborn calfserum 2ml
extract. The analysis should nOflllal.1Y include ar- Gentamicin solution (4000 l.u.mf'") 1 rol
senic, cadmium, chromium, copper; lead and 4.4 percent sodium bicarbonate buffer 3 rot
selenium. Other metals such as barium, beryllium, saturated with carbon dioxide
managanese, mercury, molybdenum, silver. tin, • The extractshall be usedin placeof distilledwater
vanadiumand zincshould be included jf known to for conducting the test.
be incorporated in the sample. The assessment
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J-S.2.2.3 All components of the medium shall be incubation for 24 h remove the tubes and examine
known to be sterile, and the medium must be the state of the tissue culture microscopically.
preparedaseptically.Store in the dark at 4°C ± 1°C.
J-S.S Assessment
J -5.2.2.4 Concentrated growth and maintenance
media.
If there is confluent growth in the tubes from the
control and the test extracts, examine the cells
These shall be prepared to the formulae given in closely for any signs of toxicity, for example ir-
J-S.2.2.1 but excludingany distilled water. Store at regular shaping of cells, signs otroundlng ocr of
4°C ± lOCI the cells in any appreciable number, or granular
inclusions.
J..S.2.2.S Trypsin solution
a) Where no initial signs of any adverse reac-
Trypsin 0.25 gm
tions are seen in the confluent cell
Phosphate buffered saline 100 ml
monolayer,there is no need to continue the
Warm to 37°C to dissolve. CheckpH 7.2 sterilize by test and the sample maybe considered free
filtration through O.22,um membrane filters. Store from cytotoxicity.
. at -200C. b) If there is any appreciable irregular shaping
or 'rounding off of the cells or granular
J-5.3 Preparation of Cell Suspension
inclusions in the cells in the test system,
Obtain a 24 h or 48 h confluent monolayer culture pour off the growth medium from each tube
of the cells in 4 oz or other suitably sized sterile and replace it with the appropriate main-
medical flat bottles with airtight closures using tenance medium prepared previously and
the growth and maintenance media specified in store at 4°C ± loe and reincubate the tubes
J-5.2.2.1 Pour the growth or maintenance medium at 37°C ±1°C. Re-examine the cells at daily
off the cells, irrigate the cellswellwith 10 ml phos- intervals for three to four days, recording
phate buffered saline, discard this and add 5 ml of the state of the culture on each occasion. If
the trypsinsolution, place the bottle on its side with the cells remain viable without increasing
the cell layer covered completely, leave for 30 signsof cytotoxicreactions, the sampIc shall
seconds at room temperature and then pour off the be considered to be free from cytotoxicity.
excess trypsin solution. Incubate the bottle at
J-5.6 Repeat Test
37°C ± 1°C for 4 to 5 min. Examine the bottle to
ensure that the cells have become detached from Ifanyof the fivetubes showanysignsof continuing
the bottle surface and if satisfactory add about 2.5 cytotoxicity at any time (except where this
ml growth medium and shake the bottle gently to cytotoxicity is caused by a failure to obtain an air-
suspend the cells.The number of viablecells per ml tight seal on the tube-indicated by a magenta
of the cell suspension maybe determined bythe use colouration of the medium in the tube) the test
of a haemacytometer counting chamber together shall be repeated for a second,and if needed a third
with the use of a suitable stain, such as Trypan Blue. 24 hours extraction.
J -5.4 Procedure J-6 METIIOD OF TES~r FOR
MICRO-BIOLOGICAL GROWfII
After 24 h, remove aliquots of the extract from each
test container and the control. Both growth and J-6.1 Preparation
maintenance media shall be prepared for each
specimen and control. Prepare culture media by Place specimensin specially-cleaned 21 wide neck-
placing 0.5 ml of either concentrated growth or ed flasks. Use empty flasks as controls. In the case
maintenance media together with 4.5 ml of either of containers, these containers shall serve as the
the test extract or control into a sterile bottle, and recipient for the inoculum and water amounting to
mix thoroughly. Store the maintenance media at not less than one litre of water, and it should be
4°e ± loe for later use. possible to cover the top with aluminium foil or
other suitable material.
s
To each bottle containin the diluted growth
medium, add at least 5 x 10 monkey kidneycells,
J -6.2 Inoculation
gently mix the contents and then transfer immedi- To each container place 100 ml of Inoculum. The
ately 1 ml aliquots of this cell suspension into five inoculum shall be a suitable source of micro-or-
sterile tubes (12 mm x 100 mm) with airtight ganisms, for example river or well water, or
closures. Cap each tube tightly and then incubate dechlorinated tap water with added organisms. The
the tubes at 37°C ± 1°C for 24 h and inclined at an inoculum shall be standardized to contain about
angle of 7.5° ± 2.5° from the horizontal. After 100·10000 coliform organisms and between 1 and
13
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IS 14399 (Part 1) : 1996
500 pseudomonas aeruginosa, some fungi and Incubate at 3~C for 48 h, count colonies which are
yeasts. Each container shall then be filled to one greenand/or fluorescentunder an ultra-violet lamp
litre with dechlorinated tap water. of 250 mm wave length to obtain a presumptive
count of pseudomonas acruginosa. Confirm the
J -6.3 Incubation and Assessment
colonies by subculture from the membrane on to
Cover each container and incubate at room milk agar, or other suitable medium, incubated at
temperature in the dark. After 3 days remove all the 41.5°C ±. O.SOC for 24 h. Growth at this tempera-
water,except 10ml from the oontaincr and refill to ture, coupled with production of a green and/or
one litre with fresh dechlorinated tap water. This fluorescent pigment and'case in hydrolysis, is con-
procedureshould be repeated twice weekly as long firmation of the organism.
as is necessary to complete the test,
J-6.4.4 Fungi and Yeasts
At weekly intervals,beginningfour weeksfrom the
initial Inoculation, withdraw samples of water from Carryout the standardmembrane filtration techni-
each flask after shatio,. just prior to the water que and place the membrane on plates containing
being changed. Examine these samples quantita- previously poured Rose Bengal Agar incoporatcd
tively for the organisms given below. Also esamine with a suitable antibiotic such as Kanamycin (100
for visiblegrowth. ,ug/ml)or aureomycin (SOJtg/ml), Incubate at room
temperature for up to 7 days.
Treat the control containers similarly.
J-6.4 Enumeration Techniques J-6.4.5 F'urtMr Tests
14
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Indian Standard
HOT PRESS MOULDED THERMOSETfING
GLASS FIBRE REINFORCED POLYESTER
RESIN (GRP) SECTIONAL WATER
STORAGE TANKS
PART 2 GUIDELINES FOR ASSEMBLY, INSTALLATION AND TESTING
FlO. 1 TYPICAL LAyOUT OF INTERNAL STAY BAR SYSTEM FOR SUPPORTING PANELS
15
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FIG. 2 TYPICAL LAyOUT OF ExTERNAL STAY BAR SYSTEM FOR SUPPORTING PANELS
4.7 The manhole hatches shall also be made of 1 Top, bottom and sides A
GRP and hinges shall be of aluminium alloy or 2 Bottom and lower tier of sides B
stainless steel. The minimum internal diameter of Top and top tier of sides A
the manhole shall not be less than 450 mm. 3 Bottom and lowest tier of sides C
Middle tier of sides n
4.8 Airvents shall be made from GRP or PVC. Top and top tier of sides A
They should be designed and constructed adequate-
6.2 Foundation/support system for the water tank
ly to prevent the entry of insects and other foreign
shall be designed keeping in view the worst loading
materials.
combination. Crossed girder system in structural
4.9 The internal ladders shall be made of steel ensuring peripherial support to each of the
aluminium alloy or GRP. The external ladders may bottom panels as shown in Fig. 4 is recommended.
be of mild steel completely coated for corrosion
protection. 7 LEAKAGE TEST
5 DIMENSIONS OF TANKS 7.1 After assembling, the tank shall be fined with
water up to the overflow level and after 48 h there
5.1 Length 'L' and breadth 'B', of water tanks shall shall be no visible sign of leakage.
be specified as mutltples of 0.5 m or 1 m.
7.2 Ifthe initial test fails, the leaksshall bestopped
5.2 Depth '0' of water tanks shall be in multiples in accordance with the tank manufacturers recom-
of 0.5 m SUbject to maximum of 3 m, mendationsand a further test carried out.
16
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IS 14399 (Part 2) : 1996
o
-CORNER JOINT
~lo o~o} 0 :
~INTERMEDIATE JOINT
3A
£
E
~
c PITCH
o
~ CORNER JOINT
d.
LINTERMEDIATE JOINT
38
o
o
~ «
~ _ _..;;;;;...--I
t 0 0 o;AI 0 I t-t O o 0 0
~_z._....a:;.-_
17
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CONCRETE
Note - The Edge of Bvery Panel Must be Resting on the Steel Structure
ANNEX K
(Clause 2, Part 2)
LIST OF REFERRED INDIAN STANDARDS
18
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IS 14399 (Parts 1 & 2): 1996
ANNEX L
(Foreword)
COMMITIEE COMPosmON
Convener
SHRIO. P. RATRA BuildingMaterials & Technology Promotion Council (Ministry of Urban
Development), NewDelhi
Membm
SHRI MUKBSH B. AMBANI Infra Industries Ltd, Mumbai
SHRI SANJA Y SHAH (Alternate)
( Conlinued on page 20 )
19
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( Continuedfrom poge 19 )
Members Representing
SHRI B. B. BHAnA Central Public Works Department (Standards &. Specifications),NewDelhi
SHRI D. D. GUPTA (Allemale)
SHRI AMrr CHOWDHARY Patton TankaLtd, Calcutta,
SHRI S. SAMADOAR (AllemDle)
CrrY ENGINEER Munidpal Corporation of Greater Mumbai, Mumbai
SHRJS. B. DANGAYACH SintexIndustries Ltd, Kalol (N. Oujarat)
SHRJRAJAN B. GULABANl (AltemIJIe)
DIRBCTOR U.P. lal Nigam, Lucknow
SHRI R. El.AMARAM Gummadi Polymers (P) Ltd, Madras
ENOINBER -IN-OUBP Engineer..in-Cbier. Branch, Army Headquarters, New Delhi
SHRIV. C. FRANCIS IPCL,Baroda
SHRI T. K. BANOOPADHYAY (AIler1uue)
SHRI RAMESH KUMAR KAPUR Rotomatic Containen Pvt Ltd, Nasik
SHRI RAMESH KUMAR KAPua Uniplas India. Ltd, NewDelhi
DR A. K.. RAy (AlIemDle )
MANAGING DIRECTOR Mls Naptha Resina &. Chemicals Pvt Ltd, Dangalore
SHRJ R. RAMBSH 1\iIa Devi Polymers (P) Ltd, Madras
SHRJ A P. RAMACHANDRAN (AllmulIe)
SHRJDINESHKUMARSAINI ResearchDesigns and Standards Organization (Ministryof Railways),
Lucknow
S~ARY GENERAL Federation of All India Rotomoulden, NewDelhi
SHRI PARVIN V. 88TH The All India PlasticsManufacturers' Association, Mumbai
SHRICHANORESH AMBANI (Alte1'nQte)
DR Y. N. SHARMA MIs Reliance Industries, Mumbai
SHRI N. K. SINGH Central Public Works Department (Central Designs Organization),
SHRI D. D. GUPTA (AlIema~) NewDelhi
SHRI S. SUNDARAM Glass Fibre TechnologyCentre, Ceat Ltd, Hyderabad
SHRI YOGESH VAKHARIA RotOD10Id (India) Pvt Ltd, Baroda
SHRI Anr KUMAR SHAH ( Alsemat« )
MRSSEEMA VAiDYA Carbon EverflowLtd, Nasik
SHRI R. S. N. DATIA (Allemole)
20
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Bureau of Indian Standards
BIS is a statutory institution established under the Bureau oJ· Indian Standards Act, 1986 to promote
harmonious development of the activities of standardization, marking and quality certification of goods
and attending to connected matters in the country.
Copyright
BIS has the copyright of all its publications. No part of these publications may be reproduced in any form
without the prior permission in writing of BIS. This does not preclude the free use, in the course of
implementing the standard, of necessary details, such as symbols and sizes, type or grade designations.
Enquiries relating to copyright be addressed to the Director (Publications), BIS.
Review of Indian Standards
Amendments arc issued to standards as the need arises on the basis of comments. Standards are also
reviewed periodically; a standard along with amendments is reaffirmed when such review indicates that
no changes arc needed, if the review indicates that changes are needed, it is taken up for revision. Users
of Indian Standards should ascertain that they are in possession of the latest amendments or edition by
referring to the latest issue of 'BIS Handbook' and 'Standards Monthly Additions'.
This Indian Standard has been developed from Doc: No. CED 3 (5444 & 5523).
------------::;~\......-------,-------
BUREAU OF INDIAN STANDARDS
Headquarters:
Manak Bhavan, 9 Bahadur Shah Zafar Marg, New Delhi 110002 Telegrams: Manaksanstha
Telephones: 323 01 31, 323 83 75, 323 94 02 (Common to all offices)
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