Pak Austria Fachhochschule: Institute of Applied Sciences and
Technology (PAF-IAST)
Department of Biological and Health Sciences
BTY-352 & BTY-352L Plant Biotechnology
Course Code: BTY-352 Semester: 6th
Credit Hours: 3 (2+1) Prerequisite None
Codes:
Instructor: Dr. Saleem Ur Rahman Class: BTY-F22
Office: B2-201.1 VoIP: -
Lecture & Lab Lab: Thursday: 09:00 am- E-mail:
[email protected] Days: 012:00 pm
Lecture: Tuesday:
12:00pm and 03:00 pm
Classroom: B2-303 Lab Consulting Hours: Monday: 02:00 PM –05:00 PM
B1-205
Knowledge Group: Biotechnology Updates on LMS: -
Course Description:
Agriculture biotechnology and its applications in crop improvements; cell and plant tissue culture
methodology; improvement of plants via plant cell culture; plant molecular biomarkers; direct and indirect
methods of plant and animal transformation: gene gun method of transformation, Agrobacterium mediated
transformation, chloroplast transformation and polyethylene glycol (PEG) mediated transformation; transgenic
crops with herbicide, biotic and abiotic stress resistance; problems related to transgenic plants; genetically
modified organisms (GMOs); field evaluation and commercialization of GMOs; possible effects of releasing
GMOs into the environment; bio-fertilizers, bio-pesticides and their types; non-symbiotic nitrogen fixers;
present and future prospects of bio- fertilizers.
Course Objectives:
• To acquaint students with techniques and skills employed for producing transgenic crops.
Course Learning Outcomes (CLOs):
At the end of the course the students will be able to:
CLO 1 Describe methods for obtaining and application of genetically modified plants
CLO 2 Define regulatory issues for genetically modified plant production
CLO 3 Explain the application of plants as bioreactors for the production of vaccines and
therapeutic proteins
Mapping of Class Learning Objectives to Assessment Modules and Weightages
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Pak Austria Fachhochschule: Institute of Applied Sciences and
Technology (PAF-IAST)
Department of Biological and Health Sciences
Exams and Grading: Minimum Four quizzes (can vary) and four assignments (can vary), one for each of the
sections into which the course is divided, will be given. These will be a mixture of multiple choice and answer
questions. Each quiz and assignment will contribute to the 20% of your final grade.
Besides the assignments mentioned in the semester lecture schedule, every student has to deliver a short
individual learning diary (“portfolio”) on weekly basis i.e, A portfolio of the topics covered in that week.
Rubrics for the assessment of the Assignments as well as weekly portfolio will be shared with the students
so that they know the criterion of their assessment.
The overall grade will be based on the sessional and mid-term exam and final Exam.
Course Grading: Relative
Assessment (amendable)
Theory Course
Final Exam Pre-Final
50% 50%
Mid Term Exam Sessional
25% 25%
The Sessional includes: Quizzes, Assignments, projects/internships, class discussions
Lab Course
Lab Exam and viva Lab Tasks
30% 70%
Assessment of practical part will be done based on physical presence of the student in the lab and
lab tasks performance. The rubrics shall be shared with the student for the lab task evaluations.
A Minimum 14 Lab tasks will be evaluated in a semester.
Books:
• Singh RL, Mondal S, 2018. Biotechnology for Sustainable Agriculture: Emerging
Recommended
Approaches and Strategies, Woodhood Publishing, USA
Books:
• 2. Qaim M, 2010. Agricultural Biotechnology in Developing Countries: Towards
Optimizing Benefits for Poor. Springer
• 3. Kemp Ken F, 2010. Genetic Modification of Plants: Agriculture,
Horticulture and Forestry (Biotechnology in Agriculture and Forestry). Springer.
• 4. Herren RV, 2012. Introduction to Agricultural Biotechnology. 2nd Edition;
Delmar Cengage Learning.
• 5. Slater A, 2008. Plant Biotechnology: The Genetic Manipulation of Plants.
2nd Edition; Oxford University Press, USA
• 6. Altman A, 2011. Plant Biotechnology and Agriculture: Prospects for the
21st Century. 1st Edition; Academic Press.
•
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Pak Austria Fachhochschule: Institute of Applied Sciences and
Technology (PAF-IAST)
Department of Biological and Health Sciences
Lecture Home/after class
activity
Week 1 Lecture-1: Introduction to agriculture/Plant Biotechnology
Lecture-2: Applications of plant Biotechnology in crop improvements
Week 2 Lecture-3: Plant cell and tissue culture
Lecture-4: Types of tissue culture; Somatic embryogenesis, Callus induction;
organogenesis etc.
Week 3 Lecture-5: Explant preparation and sterilization
Lecture-6 Tissue culture media; types; composition; preparation
Week 4 Lecture-7: Plant Cell and Tissue Culture; Lab Designing and Management Quiz-1
Lecture-8: Applications of plant cell and culture; case studies Assignment-1
Week 5 Lecture-9: Plant Hormones and Plant Growth Regulators
Lecture-10: Genetic Transformation of Plants and methods of transformation
Week 6 Lecture-11: Direct and indirect methods of plant transformation
Lecture 12: Agrobacterium mediated transformation
Week 7 Lecture-13: Factors affecting Agrobacterium-mediated transformation
Lecture 14: Chloroplast transformation
Week 8 Lecture-15: Chloroplast transformation; advantages and limitations Quiz-2
Lecture-16: Biolistic transformation; advantages and limitations Assignment-2
Week 9 MID TERM EXAM
Week 10 Lecture-17: Introduction to biotic and abiotic stress resistance
Lecture 18: Development of transgenic crops/plants
Week 11 Lecture-19: Field evaluation of Genetically modified organisms (GMOs)
Lecture-20: Problems related to Genetically modified organisms (GMOs)
Week 12 Lecture-21: An introduction to herbs, herbicides and chemical treatment
Lecture-22: Development of herbicide resistant transgenic plants
Week 13 Lecture-23: Case studies of herbicide resistant transgenic plants (Herbicide resistant
transgenic soybean as an example)
Lecture-24: The role of CRISPR/cas9 in herbicide resistance
Week 14 Lecture-25: An overview to insect resistance in plants Quiz-3
Lecture-26: Development of BT-transgenic plants for insect resistance Assignment-3
Week 15 Lecture-27: Presentations
Lecture-28: Presentations
Week 16 Lecture-29: Presentations
Lecture-30: Presentations
Week 17 Lecture-31: Development of Insect resistant transgenic plants using synthetic genes
Lecture-32: Bio-fertilizer; Present and future prospects of bio- fertilizers
Week 18 Final Exam week
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Pak Austria Fachhochschule: Institute of Applied Sciences and
Technology (PAF-IAST)
Department of Biological and Health Sciences
Lab Activity:
Course Code: BTY-325L
Credit Hour: 1
Course Title Genetic Engineering
Course Code BTY-325 L
Credit Hours 1
Pre-requisites None
Lecture Home/after
class activity
Week 1
Stocks of macronutrients, micronutrients and Hormones
Week 2 Preparation of Murashige and Skoog medium
Week 3 Preparation of Gamborg media
Week 4 Selection of ex-plant and sterilizations techniques
Week 5 Soybean seeds imbibition
Week 6 Soybean half cotyledon explants preparation
Week 7 Culturing on media
Week 8 LB media preparation
Week 9 Mid-term Exam
Week 10 Culturing of AgL1 starnd of Agrobactereium on LB media
Week 11 Streaking of AgL1colonies on LB media
Week 12 Confirmation of AgL1 strain/Phenotype
Week 13 Competent cell preparation of AGL-1 strains of Agrobacterium
Week 14 Transformation of AGL-1 strain with pCAMBIA vector
Week 15 Spreading the transformed AGLI strain on selection media
Week 16 Culturing of transformed AGL-1 strain
Week 17 Isolation of vector from transformed cells for its confirmation
Week 18 Final Exam
Grading Policy:
Quiz Policy: The quizzes may be unannounced and normally last for ten minutes. The question framed is to
test the concepts involved in last few lectures.
Assignment The course website will be the primary source for announcements and submitting assignments.
Policy:
Activity Policy: In activity week students will make the posters, brochures and e-posters to be displaced and
discussed with everyone at the campus. Group of students will deliver the seminar in lecture
theaters.
Discussion: Students will discuss the relevant topics in the group, will explore the recent and advanced
research in association with their instructors.
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Pak Austria Fachhochschule: Institute of Applied Sciences and
Technology (PAF-IAST)
Department of Biological and Health Sciences
Plagiarism: Collaboration and group work is encouraged but each student is required to submit his/her own
contribution(s). Your writings must be your own thoughts. You must cite and acknowledge all
sources of information in your assignments. Cheating and plagiarism will not be tolerated and
will lead to strict penalties including zero marks in assignments as well as referral to the Dean
for appropriate action(s).
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