Plant Tissue
Culture Project
About Us
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Plant Tissue Culture Project
Introduction:
A whole plant can be regenerated from a small tissue or plant cells in a suitable culture medium under
controlled environment. The plantlets so produced are called tissue-culture raised plants.
These plantlets are a true copy of the mother plant and show characteristics identical to the mother plant.
For example, if the mother plant is a high yielding plant the plantlets will also be high yielding. Many plant
species are presently being propagated through tissue culture successfully.
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Process of Plant Tissue Culture
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Stages of Tissue Culture Process
Preparation of
Nutrient
Medium
Establishment of
Aseptic Culture
Inoculation
Development of
plants in growth room
Hardening of Micro
Plants
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Advantages of Micro-Propagation Technology
Micro-Propagation has several advantages over conventional methods:-
1. Rapid Multiplication
2. Limited number of xplants are required
3. Uniform of true to type plants
4. Germplasm Storage
5. Disease Free Planting Material
6. Growth Manipulation
7. Round the year production
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Commercially Propagated plants in India
Plant Category Plants
• Fruits • Banana, Pineapple, Strawberry
• Cash Crops • Sugarcane, Potato
• Spices • Turmeric, Ginger, Vanilla, Large cardamom, Small Cardamom
• Medicinal Plants • Aloevera, Geranium, Stevia, Patchouli, Neem
• Ornamentals • Gerbera, Carnation, Anthurium, Lily, Syngonium, Cymbidium
• Woody Plants • Teak, Bamboo, Eucalyptus, Populus
• Bio Fuel • Jatropha, Pongamia
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Certification Required
• So as to assure quality planting material free from disease Department of
Biotechnology (DBT), Government of India has established :
• National Certification System for Tissue Culture Raised Plants (NCS-TCP).
• DBT is the Certification agency for the purpose for certification of
Tissue culture raised plants
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Establishment of Commercial Plant Tissue Culture Unit
Storage room for chemicals
It is advisable to have a separate area for storage of chemicals, apparatus and
equipment.
Washing and Media Preparation Room
The glassware washing area should be located near the sterilization room.
This area should have at least one large sink but two sinks are preferable
with running tap water. Both hot and cold water are required.
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Continues……
Inoculation Room
The most important work area is the Inoculation room where the core
activity takes place. The transfer area needs to be as clean as possible
with minimal air disturbance.
Growth Room
Culture room is an equally important area where plant cultures are
maintained under controlled environmental conditions to achieve
optimal growth. It is advisable to have more than one growth room to
provide varied culture conditions since different plant species may
have different requirements of light and temperature during in vitro
culture.
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PROJECT DETAILS
A: PROFILE OF A SELF CONTAINED UNIT:
• The project profile of a micropropagation unit with an annual production
capacity of 3 million plantlets is discussed below. A product mix of 5 different
plants has been assumed:
1. Banana Musa acuminata
2. Sugarcane Saccaharum officinarum
3. Ginger Zingiber officinale
4. Medicinal plants Chlorophytum borovillianum (Safed musli), Aloe barbadensis
5. Ornamental plants Carnation-Dianthus caryophyllus, Orchids-Vanilla
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Continue…
S.No Fixed Asset Cost(Rs.in Lakhs)
1 Land 5
2 Land Development 5.6
3 Building 35.2
4 Utilities 16
5 Equipment 69.4
6 Green and Shade House 30
7 Miscellaneous Fixed Asset 2.75
Total 163.95
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Details…
• Land: Approximate 5 acres land should be adequate for setting up a
TC unit with the above capacity. Cost of land is assumed at Rs. 5.00
Lakhs
• Green house : A green house of 7500 sq.ft. and a shade house of
80,000 sq.ft. have been assumed at a cost of Rs. 22.00 lakhs and 8.00
lakhs (total Rs. 30 lakhs) respectively. The greenhouse should be
provided with heating equipment, fans and cooling systems.
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Continues…
• Building and civil works : The building of about 8800 sq.ft includes
class 1000 clean rooms and areas with comfort AC for laboratory,
growth rooms and office space.
• The following facilities would be required in the building.
a) Storage room for chemicals
b) Washing and Media preparation room
c) Sterilization room
d) Inoculation room
e) Culture room
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Equipment
Major equipment and instruments required for the plant are as follows.
• Autoclave • Refrigerator
• Laminar air flow cabinet • Air conditioners
• Equipment for sterilization • Stereomicroscope
• Electronic weighing balance • Digital pH meter
• Water distillation apparatus • Shelves / racks
• Air handling units • Green house material
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Govt. Schemes and Incentives
• Various Central and State Government departments have framed financial
schemes and announced incentives for assistance of tissue culture industry
which are summarized below:
a. Ministry of Agriculture
b. Agricultural and Processed food products Export Development
Authority (APEDA)
c. National Horticulture Board (NHB)
d. Department of Biotechnology (DBT)
e. Small Farmers Agri business Consortium (SFAC)
f. State Level Incentives
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Call : 91-7906970928 , 91-9990787866
E-mail:
[email protected]Registered Office: Amaze Instruments , Plot
No.-E-49,Sector-6, Noida-201301 U.P
Registered Office: Amaze Instruments ,
29 Habibabad, Sarai Rehman
Aligarh - 202001
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