Bio_appendixb Constructinglinegraphs Copy
Bio_appendixb Constructinglinegraphs Copy
100 mg A 80 mg A 60 mg A 40 mg A
Figure A.1
This is the kind of picture graph that you often see in newspapers. This information can
be presented much more simply on a graph — a line graph is permissible — because our
experience tells us that when A is disappearing in a chemical reaction, it is disappearing
more or less smoothly and will not suddenly reappear. In other words, the progress of a
chemical reaction is a continuous process, and because time is a continuous process it
is permissible to relate the two kinds of information to one another on a line graph. The
procedure for constructing the line graph is shown in Figure A.2.
12.00
100 mg A
100
Mg of A Remaining
80
1.00
80 mg A 60
40
2.00
60 mg A 20
0
12.00 1.00 2.00 3.00 4.00
3.00 40 mg A
Time
Figure A.2
100
80
Mg of A Remaining
Y
60
X
40
20
0
0 1 2 3 4 5
Time (hours)
Figure A.3
It should be clear by looking at our graph that the only measurements we actually made
are those indicated by the dots. However, because the information on both scales of the
graph is assumed to be continuous, we can use the graph to find out how much A would
have been found if we had made our measurements at some other time, say 2.5 hours.
We merely locate the line that corresponds to 2.5 hours on our time scale and follow
it up until it crosses our line graph at the point X; then we look opposite X to the “Mg
of A Remaining” scale, and read off 50 mg. We conclude, then, that if we had made a
measurement at 2.5 hours, we would have found 50 mg of A left. In a similar way, we
can find out from our graph at what time a given amount of A, say 65 mg, would be left.
We have merely to find the line that represents 65 mg on the vertical scale and follow
it across until it cuts the line graph at point Y. Then we see 1.75 hours on the “Time”
scale opposite Y. This tells us that had we wished to stop the reaction with 65 mg of A
remaining, we would have had to do so after 1.75 hours.
You will notice that part of the graph has been drawn with a broken line. In making
a line graph we are properly allowed to connect only the points representing our actual
measurements. It is possible that measurements made after 3 hours will give points
that will fall on the broken-line extension of the graph, but this is not necessarily so. In
fact, the reaction may begin to slow up perceptibly, so that much less A is used up in
the fourth hour than in the third hour. Not having made any measurements during the
fourth hour, we cannot tell, and we confess our ignorance quite openly by means of the
broken line. The broken line portion of the graph is called an extrapolation, because it
goes beyond our actual experience with this particular reaction. Between any two of our
A4 Appendix B
measured points it seems fairly safe to assume that the reaction is proceeding steadily,
and this is called an interpolation. Interpolations can only be made between measured
points on a graph; beyond the measured points we must extrapolate. We know that the
amount of A remaining after 4 hours is somewhere between 0 and 40 mg. The amount
indicated by the broken line on the graph, 20 mg, is only a logical guess.
Unfortunately, it sometimes happens that even professionals take this sort of
limitation of line graphs for granted and do not confess, by means of a broken line, the
places where they are just guessing. Therefore, it is up to readers of the graph to notice
where the last actual measurement was made and use their own judgment about the
extrapolated part. Perhaps the extrapolated part fits quite well with the reader’s own
experience of this or a similar reaction, and he or she is quite willing to go along with the
author’s extrapolation. On the other hand, the reader may be interested only in the early
part of the graph and be indifferent to what the author does with the rest of it. It may
also be that the reader knows that the graph begins to flatten out after 3 hours and so
disagrees with the author. The point is that we, the readers, must be aware of what part
of the graph is extrapolated, that is, predicted, from the shape of the graph up to the time
when the measurements were stopped. Hence, you must clearly indicate on a line graph
the points that you actually measured. Regardless of what predictions or conclusions you
want to make about the graph, you must give the reader the liberty of disagreeing with
you. Therefore, it is very improper to construct a line graph consisting of an unbroken
line without indicating the experimentally determined points.
2000
Slices of Pizza
Remaining
1000
0
0 1 2 3 4 5
Duration of Party (hours)
Figure A.4
If you like pizza, it might be very useful to know when this party is being held. Without
a title, you cannot tell even whether the graph refers to any particular party at all. It
Appendix B A5
(c) An AP Biofeast!
None of those titles is especially useful or informative because none of them tells what
the graph is all about. Now look at these two titles:
You should be able to see that only title (e) is helpful and useful. It enables you to tell, by
glancing at the calendar, whether or not you can attend the party, and it helps make that
graph fall a little more steeply. The point we are driving at is that a good title is one that
tells exactly what information the author is trying to present with the graph. Although
brevity is desirable, it should not substitute for completeness and clarity.
Now that you are clear on titles, look at the graph in Figure A.5. Its title tells you that
here is some potentially useful information. The graph suggests that, at least for 2011,
there was an upper limit to the amount of time people could usefully spend in studying
for an exam, and you might wonder, for example, how long you would have had to study
to make a perfect score.
Figure A.5: Relation Between Study Time and Score on a Biology Exam in 2011
Unfortunately, however, you cannot tell, because the graph has no labels of numbers or
units the scales. Even though this graph has a descriptive and intriguing title, it is of no
use to us at all without these very important parts. Obviously, before we can take full
advantage of the information that the graph is trying to present, we need to have some
additional details.
A6 Appendix B
In Figure A.6 the additional information has been supplied, information that seems to
make the graph more useful to us in preparing for the exam.
6FRUHRQ([DP
6WXG\7LPH KRXUV
Figure A.6: Relation Between Study Time and Score on a Biology Exam in 2011
This additional information includes scales, or axes, that are carefully marked with
numbers, and labels and units that are neatly presented. Obviously, one cannot label all
the points along the axes; that would make the numbers crowd together and look sloppy.
The units should be marked at intervals that correspond more or less to the intervals
between the experimental points. The small marks, called index marks, can be drawn in
if the experimental points are very widely spaced. Most elegantly, a frame is put around
the whole graph, and index marks are placed all around. This makes it easy to lay a ruler
across the graph when interpolating between the experimental points. The diagram in
Figure A.7 summarizes some features of a good graph.
NUMBERS
INDEX MARKS
Score (no. correct out of 60)
UNITS
60
INDEX MARKS
40
0
0 1 2 3 4 5
Study Time (hours)
NUMBERS
LABELS
UNITS
Figure A.7: Relation Between Study Time and Score on a Biology Exam in 2011
Appendix B A7
100
80
Mg of A Remaining
60
40
20
0
0 1 2 3 4 5
Time (hours)
Time Course of Disappearance of
A in Process I
Figure A.8
Let us call the process represented by the graph “Process I” and consider another
reaction, “Process II,” in which A is also consumed. Suppose that we start Process II
also with 100 mg of A, and that after 1, 2, and 3 hours there are 90, 80, and 70 mg,
respectively, left. The progress curve for Process II is displayed in Figure A.9.
100
80
Mg of A Remaining
60
40
20
0
0 1 2 3 4 5
Time (hours)
Time Course of Disappearance of
A in Process II
Figure A.9
A8 Appendix B
Now, suppose we want to compare the graphs for the two processes. Because they have
exactly the same scales, we can put both lines on the same graph, as shown in Figure
A.10. Notice, however, that now in addition to the labels on the scales, we need labels on
the two lines to distinguish between the two processes.
Look at the 1-hour mark on the time scale of the graph. Opposite this put an X on the
line for Process I and a Y on the line for Process II. Then, opposite X on the ordinate you
should be able to see that 80 mg of A are left in Process I; opposite Y you can see that 90
mg of A are left in Process II. Apparently, Process I has used up 20 mg of A and Process
II has used up only 10 mg in the same amount of time. Obviously, Process I is faster, and
the line graph for Process I is steeper than the graph for Process II.
100
80
Mg of A Remaining
Process II
60
40
Process I
20
0
0 1 2 3 4 5
Time (hours)
Time Course of Disappearance of
A in Process I and II
Figure A.10
The rate for Process I is 20 mg A used/hr, while the rate for Process II is 10 mg A
used/hr.
We have seen that a steeper line graph means a faster reaction when the progress
curves for two reactions are plotted on the same scale. (Obviously, if the progress curves
are plotted on different scales, we cannot compare the steepness of the line directly, but
have to calculate what the slope would be if the two curves were plotted on the same
scale.)
Suppose, now, that we make a new kind of graph, one that will show the steepness, or
slope, of the progress curve. Because the slope of the progress curve is a measure of the
speed of velocity, or rate of the reaction or process, such a graph is frequently called a
“rate graph” or “rate curve.” The diagram in Figure A.11 shows how a rate curve can be
made for Process I.
Appendix B A9
Mg of A Used/Hour
60 in second hour
20 mg A used up
40 in third hour 40
20 20
0 0
0 1 2 3 4 5 0 1 2 3 4 5
Time (hours) Time (hours)
Figure A.11
Notice that the time scale of this rate graph is exactly like the time scale of the progress
curve from which it was derived, but that the ordinate is different. The ordinate of the
progress curve shows milligrams of A remaining; the ordinate of the rate curve shows
milligrams of A used per hour. Obviously, a rate graph must always show rate on one
of its scales, and it is ordinarily the vertical one that is used. This is because the rate of
a reaction or process is what mathematicians call a dependent variable. Time is the
independent variable in this experiment; it is independent of changes in the dependent
variable (the rate of reaction), and it is the variable that is shown on the horizontal axis.
Regardless of whether the process is the increase in height or weight of a plant, or the
using up or producing of something in a reaction, the rate graph for the process must
always show amount of something per unit time on one of its axes. One very common
type of rate graph is the one shown in Figure A.11, with a rate on the ordinate and the
time on the abscissa. Other kinds of rate graphs may have temperature or molarity on
the abscissa. The rate of growth of a plant, for example, depends on how many factors
that we might wish to vary, and so we can have as many different kinds of rate graphs for
that process as there are independent variables.
Let us emphasize: a progress curve always shows amount of reaction on the vertical
scale and time on the horizontal scale. The corresponding rate curve may show time
or some other variable on the horizontal scale, but it always shows rate, or amount of
reaction per unit time, on the vertical scale. This point is very important. When we look
at a rate curve that has time on the horizontal scale, we must visualize the progress curve
from which the rate curve was derived. When we look at a rate curve that has any other
variable except time on the horizontal scale, we shall see that each point on the rate
curve represents a separate progress curve.
In the same way as for Process I, a rate curve can be made for Process II. Plotted on
the same graph, the two should look something like the diagram in Figure A.12.
A10 Appendix B
10 Process II
0
0 1 2 3 4 5
Time (hours)
Figure A.12
There are two things to notice in this example. First, the curve for Process I lies higher
than that for Process II. This is in accord with the facts as we have seen them, namely,
that Process I is faster and so has a greater slope or higher value for the steepness.
Second, notice that both curves are perfectly flat. Naturally, because the progress curves
for the two processes were both perfectly straight lines, having everywhere the same
slope, the rate of steepness graph must show exactly the same thing, that is, that the rate
or steepness is everywhere the same.
On the other hand, consider the graph in Figure A.13, which represents the
disappearance of A in yet another reaction, Process III.
100
40 mg A used up
Mg of A Remaining
80 in first hour
10 mg A used up
60 in second hour
10 mg A used up
40 in third hour
5 mg A used up
in fourth and
20 fifth hours
Mg of A Used/Hour
0
0 1 2 3 4 5
40
Time (hours)
20
0
0 1 2 3 4 5
Time (hours)
You can see that Process III differs from Processes I and II in that the progress curve for
III is not a perfectly straight line. It is steepest at the beginning, becomes less steep after
1 hour, and again after 3 hours. Obviously, because the rate of the process is changing
with time, the corresponding rate curve will not be perfectly flat. The rate has to start
out high, then drop at 1 hour and at 3 hours, and you can see in the graph on the right
Appendix B A11
C
L
10
Time (hours)
Figure A.14
Suppose we want to measure the slope, or steepness, of the curved line C at time 2 hours.
We can see that the curve rises 5 units total in the 2 hours, so that the average slope is
2.5 units per hour. However, it is easy to see from the graph that this average is very
misleading; the progress curve is almost flat at the beginning (i.e., has 0 slope) and then
accelerates rapidly, so that the line curves upward. If we want to find the true slope at 2
hours, we must draw line L in such a way that L has the same slope as C at the 2-hour
point. Then we can see that L rises about 5 units between 1 and 2 hours, just twice the
average slope for the first 2 hours.
We have seen that a perfectly flat curve, like that for Process I or II, means that the
corresponding progress curve is a perfectly straight line having the same slope at all
points. Conversely, a progress curve that changes in slope, like that of Process III, will
give a rate curve that looks like steps. You should be able to figure out that the “steps”
on the rate curve will be sharp and square if the progress curve has an abrupt change in
slope, and more rounded off if the progress curve changes slope gradually. In any case, in
regions where the rate curve is perfectly flat it is clear that the progress curve must have
constant steepness, or slope. However, if the progress curve itself gets perfectly flat, then
that portion of the progress curve has 0 slope; in other words, the reaction has stopped.
This kind of situation is pictured in Figure A.15 where the rate and progress curves for
another reaction, call it Process IV, are shown.
A12 Appendix B
100
Mg of A Remaining
80
60 60
Mg of A Used/Hour
40 40
20 20
0 0
0 1 2 3 4 5 0 1 2 3 4 5
Time (hours) Time (hours)
Progress Curve Rate Curve for the Disappearance
of A in Process IV
Figure A.15
In the progress curve on the left, we can see that after the first hour the reaction stopped.
From the graph we can see that after 1 hour there were 50 mg of A remaining; after 2
hours there were still 50 mg remaining; and there are still 50 mg remaining even at 4
hours. Obviously, Process IV stopped when one-half of A had been used up. Now look
at the rate curve on the right. It is perfectly flat for the first hour because the slope of the
progress curve was constant during that time. After the first hour the rate curve is also
perfectly flat but it has dropped down to 0, indicating that although the progress curve
has constant slope, the slope is actually 0. Obviously, flatness in a rate curve and flatness
in a progress curve mean different things. Flatness in the progress curve for a reaction
means that the reaction has stopped; flatness in the rate curve means that the reaction is
going on at a constant rate. You can see, then, that we have to be able to glance at a graph
and tell whether it is a rate curve or a progress curve in order to be able to interpret what
the shape of the curve is trying to tell us.
Now let us take one more example of this kind of rate curve. The graph in Figure A.16
shows the progress in the growth of a pea plant. First, we can see that the slope is not the
same everywhere. In fact, there is an interval where the slope increases very gradually
from 0. By 1 week or so the slope has reached its maximum value and is steady until
about 3 weeks. Thereafter, the slope begins to decrease again, as the curve bends over,
and eventually, at about 4.5 weeks, as the curve gets perfectly flat, the slope, or steepness,
tends to be 0 again.
Appendix B A13
60
20
0
0 1 2 3 4 5
Time (weeks)
Progress Curve for the
Growth of a Pea Plant
Figure A.16
Suppose, now, that we try to imagine what the rate curve for the growth of this pea
plant will look like. If you read through the preceding paragraph, you will have a rough
description of it. In fact, it will look like the graph in Figure A.17.
15
Growth Rate (cm/week)
10
0
0 1 2 3 4 5
Time (weeks)
Growth Rate of a Pea Plant
Figure A.17
Notice from the two graphs that where the steepness of the progress curve gets larger, the
corresponding rate curve turns upward. Similarly, when the slope of the progress curve
decreases again, the rate curve turns downward. A rate curve that is turning up means,
therefore, that the process is speeding up; a flat rate curve means that the process is going
at a constant rate; and a rate curve that is turning down means that the process is slowing
down. When the rate curve hits the x-axis, it means that the reaction has stopped.
Probably 80 percent of the graphs you will encounter in biology are either rate curves
or progress curves. You will have noticed from the preceding discussion that biologists
tend to use the words “graph” and “curve” interchangeably. Technically, of course, the
entire picture, including the abscissa, ordinate, labels, numbers, units, index marks, and
title, together with the line graph portrayed, is the “graph,” while the line graph itself is
called the “curve.” You will notice, too, that biologists call a line graph a “curve,” even
though the line itself may be perfectly straight.
A14 Appendix B
100 30°C
80
Mg of A Remaining
40°C
60 20°C
40
10°C
20 50°C
0
0 1 2 3 4 5
Time (hours)
Temperature Dependence of Process I
Figure A.18
Before we try to construct the rate curve for this graph, we should try to imagine how
this experiment was carried out. It seems clear that the experiment must have started
with several different batches of A and that each reaction mixture was kept at a different
temperature. Then, every half-hour, the amount of A remaining was measured and the
amount consumed was calculated. The results might have been plotted in five separate
progress curves, as shown in Figure A.19.
Appendix B A15
Mg A Consumed
60
40
20
0
0 1 2 3 0 1 2 3 0 1 2 3 0 1 2 3 0 1 2 3
Time (hours) Time (hours) Time (hours) Time (hours) Time (hours)
Figure A.19
When all these progress curves are plotted on the same graph, as was done in Figure
A.18, we have what is called a “family” of curves. If we look at the slopes of the various
members of the family of curves for Process I, we see that the steepest slope does not
correspond to the highest temperature. In fact, the curve for 30° is the steepest, whereas
the curve for 50° is the least steep; the curve for 10°, the lowest temperature, has an
intermediate slope. By analyzing and comparing the slopes of the family of curves in
this way we can get a reasonably good notion of the effect of temperature on Process I,
but this effect could be shown much more clearly in a rate graph that has temperature
as the abscissa. Such a graph would show us at a glance how the rate varies with
temperature and, of course, would be preferable, as the whole point in making a graph is
to present information simply and clearly. The diagram in Figure A.20 shows how a rate-
temperature graph would be constructed from this family of curves for Process I.
100
30°
80 40°
20°
60
This much in 1 hr at 30°
40 10°
This much in 1 hr at 40°
This much in 1 hr at 20°
20 50°
This much in 1 hr at 10°
This much in 1 hr at 50°
0
0 1 2 3 4
Mg A Consumed/Hour
Time (hours)
60
40
20
0
0 10 20 30 40 50
Temperature (°C)
Figure A.20
Having found, as shown in Figure A.20, the five points for our rate graph, we are faced
with the question of whether or not it is legitimate to connect these points with a
A16 Appendix B
smooth line. We recognize, of course, that both temperature and rate are continuous
processes. Between any two given temperatures or rates there are an infinite number
of temperatures or rates. The question here, however, is the following: If we do draw a
smooth line through our five points, will that line pass through the infinite number of
other rates that we could have measured if we had chosen some other temperature? Let
us go ahead and draw the line, as shown in Figure A.21
60
Mg A Consumed/Hour
40
20
0
0 10 20 30 40 50
Temperature (°C)
Figure A.21
As we have drawn it, the curve indicates that the rate at 29° and at 31° would be slightly
lower than at 30°, and this may not be true. In order to determine the true shape of
the curve in the region of the maximum rate we would have to make progress curves
at smaller temperature intervals, say, every two degrees. However, it is extremely
unlikely that the true shape of the curve is anything like the two possibilities shown on
the diagrams in Figure A.22. All our experience tells us that if a reaction depends on
temperature, then that dependence will be a smooth curve, without sharp bends. In
fact, if in an experiment we should observe behavior of the type shown in Figure A.22,
we would immediately begin to suspect that something is wrong with our thermostat!
Thus, although it may be that the shape of the rate-temperature curve for Process I is
somewhat different from the way we drew it in Figure A.21, we can be reasonably certain
that it is not radically different.
60 60
Mg A Consumed/Hour
Mg A Consumed/Hour
40 40
20 20
0 0
0 10 20 30 40 50 0 10 20 30 40 50
Temperature (°C) Temperature (°C)
Figure A.22
In addition, we may also tend to be suspicious of a graph if we see a sharp peak, unless
the experimental points were taken very close together. For example, common sense
would tell us to be careful about accepting the rate curve shown in Figure A.23.
Appendix B A17
Mg A Consumed/Hour
80
40
0
0 20 40 60 80 100
Temperature (°C)
Figure A.23
Obviously, most of the shape is given to the profile by the one measurement at 60°. In
biology, as in everything else, mistakes can be made, so the experimenter would have to
check the validity of that measurement very carefully. The easiest way to do that would
be to make more measurements slightly above and slightly below 60° to see whether
these would fall on the line the experimenter has drawn. Alternatively, the experimenter
could play it safe and draw only a bar graph for these spaced temperatures. Another
useful dodge would be to connect the points with a smooth but broken line rather than
a continuous line. As always, the broken line would suggest the tentative and provisional
nature of the curve as drawn.
A18 Appendix B