Histopathology & Fundamental Of
Applied Histopathology
Topic - 2
Laboratory Equipment: Uses and
Maintenance ?
Laboratory equipment is essential for
conducting experiments, research, and
diagnostics. Proper use and
maintenance ensure accuracy,
longevity, and safety. Below is a
detailed guide on common lab
equipment, their uses, and maintenance.
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1. Common Laboratory Equipment,
Uses, and Maintenance**
1.1. Glassware
a. Beakers
Uses:-- Measuring, mixing, and heating
liquids.
Maintenance:-
- Wash with distilled water and lab
detergent after use.
- Avoid sudden temperature changes
to prevent cracking.
- Store upright to prevent
contamination.
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b. Test Tubes
Uses:-- Holding, mixing, and heating
small liquid samples.
Maintenance:--
- Rinse immediately after use to
prevent residue buildup.
- Use a test tube brush for thorough
cleaning.
- Avoid direct flames unless heat-
resistant
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C. Volumetric Flasks
Uses:-- Precise measurement and
preparation of solutions.
Maintenance:--
- Wash with a cleaning solution and
rinse with distilled water.
- Store with a stopper to prevent
contamination.
- Do not use abrasive cleaners to avoid
scratches.
D. Pipettes and Burettes
Uses:--Accurate liquid measurement
and titrations.
Maintenance:--
- Rinse before and after use with the
appropriate solvent.
- Avoid air bubbles when using.
- Store in a clean, dust-free area.
1.2. Measuring Instruments:-
a. Balances (Analytical and Digital)
Uses:-Weighing chemicals with
precision.
Maintenance:-
- Calibrate regularly for accurate
readings.
- Keep free from dust and moisture.
- Avoid overloading beyond capacity.
b. Thermometers
Uses:- Measuring temperature in
experiments.
Maintenance:-
- Handle gently to prevent breakage.
- Clean with alcohol after use.
- Store upright in a protective case.
1.3. Heating Equipment
a. Bunsen Burner
Uses:- Heating, sterilization, and
combustion.
Maintenance:-
- Inspect for gas leaks before use.
- Clean the nozzle regularly to prevent
clogging.
- Turn off the gas supply after use.
b. Hot Plate
Uses:-- Heating solutions in glassware.
Maintenance:--
- Clean spills immediately after cooling.
- Avoid overloading with heavy
containers.
- Check electrical connections
periodically.
c. Water Bath
Uses:-Maintaining a constant
temperature for samples.
Maintenance:-
- Change water regularly to prevent
contamination.
- Clean with a disinfectant weekly.
- Avoid overfilling to prevent spillage.
1.4. Microscopes
Uses:-- Magnification of small objects
like cells and bacteria.
Maintenance:--
- Cover with a dust cover when not in
use.
- Clean lenses with lens paper and
ethanol.
- Store in a dry, stable place.
---
1.5. Centrifuges
Uses:-- Separating substances based
on density.
Maintenance:--
- Balance samples before spinning to
prevent damage.
- Clean rotor and chamber after each
use.
- Inspect for wear and tear regularly.
---
1.6. Autoclaves
Uses:-- Sterilizing lab equipment and
waste.
Maintenance:-
- Check the water level before each
use.
- Descale periodically to prevent
mineral buildup.
- Follow safety procedures to avoid
pressure hazards.
1.7. pH Meters
Uses:--Measuring the acidity or
alkalinity of solutions.
Maintenance:-
- Calibrate before each use.
- Store the electrode in a proper
storage solution.
- Avoid dry storage to prevent damage.
2. General Laboratory Equipment
Maintenance Guidelines
1. Regular Cleaning:-- Prevents
contamination and ensures accurate
results.
2.Proper Storage:--Protects from dust,
moisture, and damage.
3.Calibration and Testing:--Ensures
precision in measurements.
4.Scheduled Servicing:-- Prevents
malfunction and prolongs equipment
life.
5.Careful Handling:- Reduces breakage
and wear.
6.Labeling and Documentation:-- Helps
track maintenance schedules and
replacements.
By following proper usage and
maintenance protocols, lab equipment
remains reliable, safe, and effective.
Thanks For Watching
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