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CRP Latex Test Guide

The document outlines the CRP (C-Reactive Protein) test using the latex agglutination method, detailing its biological importance, sampling techniques, and testing principles. It discusses the equipment, reagents, and technical steps involved, along with factors influencing the technique and limitations of the test. Additionally, it provides information on result interpretation, quality control measures, and proposed improvements for the testing process.

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etoundirosaire237
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30 views2 pages

CRP Latex Test Guide

The document outlines the CRP (C-Reactive Protein) test using the latex agglutination method, detailing its biological importance, sampling techniques, and testing principles. It discusses the equipment, reagents, and technical steps involved, along with factors influencing the technique and limitations of the test. Additionally, it provides information on result interpretation, quality control measures, and proposed improvements for the testing process.

Uploaded by

etoundirosaire237
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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CRP (C-Reactive Protein) Test - Latex Agglutination Method

1. Biological Importance

CRP is an acute-phase protein produced by the liver in response to inflammation...

2. Sampling Techniques

Sample type: Venous blood. Tube: Plain tube or with serum separator...

3. Principle of the Test

The test is based on latex agglutination: Latex particles are coated...

4. Equipment Used

Latex-coated test kit, agglutination card, micropipettes, centrifuge...

5. Reagents

Latex suspension with anti-CRP, positive and negative controls, saline...

6. Technical Steps (Qualitative & Semi-Quantitative)

Qualitative: Mix serum and latex, observe for agglutination...

7. Factors Influencing the Technique

Hemolysis, improper mixing, temperature variations...

8. Limitations of the Test

Not specific, limited sensitivity, can't distinguish infection types...

9. Reading the Results

Qualitative: presence/absence of agglutination. Semi-quantitative: dilution method...


CRP (C-Reactive Protein) Test - Latex Agglutination Method

10. Expression of Results

Qualitative: Positive/Negative. Semi-Quantitative: mg/L or mg/dL...

11. Reference Values

Normal CRP: < 6 mg/L. Mild: 6-30. Moderate: 30-100. Severe: >100 mg/L...

12. Interpretation of Results

Low CRP: normal/viral. High CRP: bacterial or severe inflammation...

13. Physiological and Pathological Variations

Pregnancy, aging, infections, cancer...

14. Quality Control

Run controls with each batch, use IQC charts, calibrate instruments...

15. Limits & Proposed Alternatives

Semi-quantitative only, subjective reading, alternative methods like immunoturbidimetry...

17. Other Techniques

Immunoturbidimetry, nephelometry, hs-CRP, ELISA, POCT...

16. Step-by-Step Analysis & Proposed Improvements

Step Issue Proposed Improvement

Sample handling Hemolysis risk Train in venipuncture, use proper tubes

Latex mixing Manual error Use rotator or automation

Reading Subjective Use optical reader for clarity

Reagent stability Temperature sensitivity Store at 2-8°C, monitor expiry dates

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