NAME………………………………………………………. STRM………ADM…………..

DATE……. ……………………. SIGN…….…………………….

231/3
BIOLOGY PRACTICAL
PAPER 3
June 2022
Time: 1 ¾ Hours

KASSU JET EXAMINATION 2022

INSTRUCTIONS TO CANDIDATES
 Answer all the questions in the spaces provided.
 You are required to spend the first 15 minutes of 1 ¾ hours allowed for this paper reading the
whole paper carefully before commencing your work.
 Candidates may be penalized for recording irrelevant information and for incorrect spelling
especially of technical terms.

FOR EXAMINER’S USE ONLY

Question Max Score Candidate’s Score

TOTAL

This paper consists of ............. printed pages. Candidates should check the question paper to
ensure that all pages are printed as indicated and no questions are missing.

1
1. a) (i) You are provided with a pestle, mortar, scapel specimen Q and R,. Cut from each a
cube, each measuring 1cm by 1cm. put them each in a different test tube having
10mls of solution X. Record the observations in the table below? (2 marks)

Specimen Observation

Specimen Q

Specimen R

(ii) Account for the observations in the experiment involving specimen Q and R?
(3
marks)
………………………………………………………………………………………………
……………………………………………………………………………………...............
……………………………………………………………………………………………
………………………………………………………………………………………..........
b) i) Using the remaining portion of specimen Q. Cut 2 other pieces measuring 1cm by
1cm ,Crush them separately to form a paste and put them in boiling tubes labeled A
and B.

To the paste in boiling tube labeled A, add 5mls of solution X .Record the
observation in the table below.

To the paste in boiling tube labeled B add 10mls of distilled water and boil for
5 minutes then allow it cool then add 5mls of solution X. Record the observation
in the table below? (2 marks)

BOILING TUBE OBSERVATION

2
ii) Account for the observations in the experiment involving boiling tube A and B?
(4
marks)
Boiling tube A
………………………………………………………………………………………
………………………………………………………………………………………
……............................................................................................................................
Boiling tube B
………………………………………………………………………………………
………………………………………………………………………………………
……...........................................................................................................................
iii) Name the biological substance being investigated and its significance to the living
tissue? (2 marks)
Biological substance
……………………………………………………………………………………….
Significance
………………………………………………………………………………………
………………………………………………………………………………………
……...........................................................................................................................
iv) Name the factor being investigated in question 2(b) above (1mark)
………………………………………………………………………………………

3
231/3
BIOLOGY PRACTICAL
PAPER 3
June 2022

KASSU JET EXAMINATION 2022

1. a. (i) You are provided with a pestle, mortar, scapel specimen Q and R,. Cut from each a
cube, each measuring 1cm by 1cm. put them each in a different test tube having
10mls of solution X. Record the observations in the table below? (2marks)

Specimen Observation
Specimen Q A lot of bubbling / Effervescence / Fizzling
Specimen R Little bubbling / less effervescence / little fizzling

(ii) Account for the observations in the experiment involving specimen Q and R?

(3marks)
There is more bubbling / more gas produced in Q (liver) than in R banana; since
there is more metabolic activities in Q than in R; Animals are more active than
plants;

b) i) Using the remaining portion of specimen Q. Cut 2 other pieces measuring 1cm by
1cm ,Crush them separately to form a paste and put them in boiling tubes labeled A
and B.
To the paste in boiling tube labeled A, add 5mls of solution X .Record the
observation in the table below. .
To the paste in boiling tube labeled B add 10mls of distilled water and boil for
5minutes then allow it cool then add 5mls of solution X. Record the observation in
the table below? (2marks)

BOILING TUBE Observation

(ii) Account for the observations in the experiment involving boiling tube A and B?

(4marks)
Boiling tube A
Crushing increases surface area for enzyme catalase; rapid / faster
metabolism / breakdown of hydrogen peroxide occurs

Boiling tube B

4
 Boiling denatures the cells / enzyme catalase; reduces enzyme activity /
metabolism / substrate breakdown.

(iii) Name the biological substance being investigated and its significance to the
living tissue? (2marks)

Biological substance
Catalase/enzyme

Significance
Detoxification/breakdown harmful substance/hydrogen peroxide

iv) Name the factor being investigated in question 2(b) above (1mark)
Effect of temperature on enzyme action