P/N 679046

Revision 1.0

GS-15/R / Allegra 21/R

Avanti 30 / Allegra 64R
Spinchron 15/R

Service Manual

Beckman Coulter, Inc.

250 South Kraemer Blvd.
Brea, CA
92821

GS15/R / Allegra21/R / Avanti 30/ Allegra 64R / Spinchron 15/R Service Manual 6/13
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GS15/R / Allegra21/R / Avanti 30/ Allegra 64R / Spinchron 15/R Service Manual 6/13
 GS-15/R/ Allegra 21/R, Avanti 30/ Allegra 64R, Spinchron 15/R Service Manual
Table of Contents
REVISION HISTORY .................................................................................................................................... 3
MANUAL USE .............................................................................................................................................. 3
RELATED DOCUMENTS ............................................................................................................................. 3
SAFETY INFORMATION.............................................................................................................................. 4
1 SYSTEM DESCRIPTION ..................................................................................................................... 9
1.1 Safety Features .......................................................................................................................... 9
1.2 GS-15 / Spinchron 15 (Zent2) / Allegra 21 Specifications.................................................. 12
1.3 Avanti 30/ Allegra 64R Specification ..................................................................................... 13
2 INSTALLATION ................................................................................................................................. 17
2.1 Safe Centrifuge Operation ...................................................................................................... 17
2.2 Installation Requirements ....................................................................................................... 18
2.2.1 Rotor Attachment................................................................................................................... 19
2.2.2 Customer Training and Instrument Demo ............................................................................. 21
3 SERVICE PROCEDURES ................................................................................................................. 23
3.1 GS-15, Allegra 21, Spinchron 15R Service Procedures ....................................................... 23
3.1.1 Door Latch ............................................................................................................................. 23
3.1.2 Emergency Release of the Door Latch ................................................................................. 23
3.1.3 Latch Replacement ............................................................................................................... 23
3.1.4 Drive Assembly...................................................................................................................... 24
3.1.5 Drive Suspension .................................................................................................................. 24
3.1.6 Drive Removal ....................................................................................................................... 25
3.1.7 GS15/R, Allegra 21/R, Spin 15/R Imbalance ........................................................................ 26
3.1.8 Cooling .................................................................................................................................. 27
3.1.9 Refrigeration Cooling ............................................................................................................. 28
3.1.10 Servicing the Refrigeration System ................................................................................... 30
3.1.11 Cooling System Performance ........................................................................................... 30
3.1.12 Calibration of the Refrigeration System ............................................................................ 31
3.1.13 Chamber Gasket Replacement GS15, Allegra 21, Spin 15 .............................................. 32
3.1.14 Electronics ......................................................................................................................... 33
3.2 Avanti 30, Allegra 64R ............................................................................................................. 36
3.2.1 Door latch .............................................................................................................................. 36
3.2.1.1 Door Latch Replacement .............................................................................................. 36
3.2.2 Drive Assembly...................................................................................................................... 36
3.2.2.1 Drive Suspension .......................................................................................................... 36
3.2.3 Gray Wedge Filter Imbalance Sensor (Avanti 30, Allegra 64R only) .................................... 37
3.2.3.1 Imbalance Adjustment Instructions (Avanti 30, Allegra 64R) ....................................... 39
3.2.3.2 Remove sensor by pulling after removing RTV ............................................................ 41
3.3 Service Mode Zent Version Electronics ................................................................................ 42
4 GS-15/R/ ALLEGRA 21/R, SPINCHRON 15R, AVANTI 30/ ALLEGRA 64R CIRCUIT
DESCRIPTION ............................................................................................................................................ 50
4.1 Circuit Description Block Diagrams Zent 2 Version Electronics ....................................... 50
4.1.2 Power Board .......................................................................................................................... 51
4.1.3 Electronic Speed Regulations for Centrifuge Drives ............................................................. 51
Main Input ........................................................................................................................................... 52
Relay Control ...................................................................................................................................... 52
Frequency Converter ..................................................................................................................... 53
Frequency Converter .......................................................................................................................... 53
Brake Chopper ................................................................................................................................... 54
Power Supply ..................................................................................................................................... 54
5 SERVICE PARTS............................................................................................................................... 55
5.1 Allegra 21 .................................................................................................................................. 55
5.2 Allegra64R ................................................................................................................................ 58
Electrical ................................................................................................................................................ 58
Mechanical............................................................................................................................................. 59
Refrigeration.......................................................................................................................................... 59

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Table of Contents
Tools and Supplies ............................................................................................................................... 60

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Revision History

Revision History
Rev. Date Responsible Description
1.0 6/18/2013 Horst Krischer First release of new complete version of the manual.

Manual Use
This Service Manual is copyrighted and all rights reserved by Beckman Coulter, Inc. This document may
not in whole or part be copied, photocopied, reproduced, translated or reduced to any electronic medium or
machine readable form without prior consent in writing from Beckman Coulter Inc.

This Service Manual is designed solely for the use of Beckman Coulter Field Service Representatives who
have training and experience in the servicing of Beckman Coulter products. To improve product
performance, Beckman Coulter may have made changes in the product to which this Service Manual
relates. Such changes may not be reflected in this manual. Accordingly, Beckman Coulter makes no
representation or warranty, either express or implied, that the information contained in this Service Manual
is complete or accurate with respect to the particular product to which this manual pertains, and the
purchaser must assume all risks in the use of this manual for the purpose of performing service upon such
product.

Because of the possible hazards involved to an inexperienced person in servicing the product,
Beckman Coulter recommends that servicing of the product be performed only by Beckman
Coulter Field Service Representatives.

Components of this instrument which are considered user serviceable are discussed in detail in the
instrument's Instruction Manual.

Related Documents
This Service Manual does not include the Instruction Manual for this instrument. The Instruction Manual
contains instructions for proper operation of the instrument, information regarding the limitations,
specifications, precautions and hazards associated with its use.

To obtain a copy of the Instruction Manual, please order it through any Beckman Coulter Sales and Service
Office.

Part Number Description

GS15-IM-8 Allegra 21, GS-15, Spinchron 15 Series Instruction Manual
GS30–IM-11Allegra 64, Avanti 30 Instruction Manual

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Safety Information
Safety Reminders

The following pages summarize cautionary information basic to the safe operation of this instrument.
However, it is strongly recommended that the user read the entire manual carefully before attempting to
service the instrument. In addition, be sure to heed all NOTES, CAUTIONS, and WARNINGS, which
are specifically defined as follows:

NOTE: Used to make a procedure easier or more understandable. To disregard it may cause
inconvenience, but not mechanical damage or personal injury.

CAUTION: Used to prevent equipment damage. To disregard the caution may cause mechanical
damage however, personal injury is not likely.

WARNING: Used when an action or circumstance may potentially cause personal injury. Damage may
also result.

WARNING: If the equipment is used in a manner not specified by Beckman Coulter, Inc.,
the protection provided by the equipment may be impaired.

Electrical Safety
To prevent electrically related injuries and property damage, properly inspect all electrical equipment prior
to use and immediately report any electrical deficiencies. Contact a service representative for any servicing
of equipment requiring the removal of covers or panels.

Avoid standing on damp floors. Disconnect the power cord before conducting any mechanical service on
the instrument. Disconnect the power cord before working on high voltage circuitry.

High Voltage

This symbol indicates the potential of an electrical shock hazard existing from a high voltage source and
that all safety instructions should be read and understood before proceeding with the installation,
maintenance, and servicing of all modules. Turn the POWER off and disconnect the instrument from the
main power source before performing any maintenance that requires the removal of an instrument panel.

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Electrostatic Discharge

Check the grounding connections on the wrist strap and static mats. Ensure mat has proper ground.
Touch conductive work surfaces before starting work. Do not allow anyone not grounded to touch ESD
sensitive components.

Do not touch the components by pins or leads. Boards should not be handled by anyone not properly
grounded.

Keep paper, non conductive plastic and plastic foams away from circuit boards and static free work areas.
Keep hand creams and food away from conductive work surfaces.

Take care when using soldering irons around conductive surfaces. Solder spills and heat from the gun may
melt and damage the conductive mat.

Mechanical Safety

Moving Parts
To avoid injury due to moving parts, observe the following:
• Under no circumstances attempt to slow or stop the rotor by hand.
• Never attempt to exchange labware, rotors, or tools while the instrument is operating.
• Never attempt to physically restrict any of the moving components of the Airfuge.
• Remember to keep clothing and fingers away from rotating or moving components. It is easy for
tools and hands to slip or get cut while servicing the instrument if caution is not exercised.
• Do not lean on the instrument during its operation.

Cleaning
Observe any cleaning procedures outlined in the Instruction Manual for the Airfuge. Prior to cleaning
equipment that has been exposed to hazardous material:
• Contact appropriate Chemical and Biological Safety personnel.
• Review the Chemical and Biological Safety information contained in the Instruction Manual.

Maintenance
Maintenance specified in this manual should be performed only by factory trained service representatives.

After mechanical repairs are complete always ensure all mounting hardware is in place and that any safety
shields or devices are installed.

Chemical and Biological Safety

It is the user’s responsibility to have the components of the Airfuge decontaminated

before requesting service by a service representative or returning parts for repair.
Beckman Coulter will NOT accept any items which have not been decontaminated
where it is appropriate to do so. If any parts are returned, they must be enclosed in
a sealed plastic bag stating that the contents are safe to handle and are not contaminated.

Do not use or service the centrifuge in the vicinity of flammable liquids or vapors. Do not operate
the instrument with flammable liquids in the rotor.

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Normal operation of the Airfuge may involve the use of materials that are pathogenic, toxic, radioactive or
otherwise biologically harmful. When such materials used, observe the following precautions:

• Contact the customer prior to any service to determine if any material used by the customer
represents a toxic, pathological or radioactive hazard.
• Ensure the instrument is sterilized or decontaminated prior to service. Customer is responsible
for any decontamination process.
• Ensure the proper personal protective equipment is used prior to servicing the instrument.
• Observe all cautionary information provided by the customer or laboratory safety officer.
• Dispose of all waste fluids (vacuum pump and diffusion pump oil) according to the facility’s waste
disposal procedures.
• Operate the Airfuge in accordance with the instructions outlined in the Instruction manual, and
take all the necessary precautions when using pathological, toxic, or radioactive materials.
• Splashing of liquids may occur; therefore, take appropriate safety precautions, such as using
safety glasses and wearing protective clothing, when working with potentially hazardous liquids.
• Use an appropriately contained environment when using hazardous materials.
• Observe the appropriate cautionary procedures as defined by a safety officer when using
flammable solvents in or near a powered-up instrument.
• Observe the appropriate cautionary procedures as defined by a safety officer when using toxic,
pathological, or radioactive materials.

NOTE: Observe all warnings and cautions listed for any external devices attached or used during
operation of the Airfuge.

CUSTOMER OPERATING INSTRUCTIONS

The Airfuge Instruction Manual is shipped as part of the instrument and is not included in this service
manual. Additional copies of the "Airfuge Instruction Manual" may be ordered from Technical Publications,
250 Kraemer Blvd., Brea, CA 92821

It is strongly recommended that the user read the entire instruction manual carefully before attempting to
operate the instrument.

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Waste Electrical and Electronic Equipment Directive

This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
directive of the European Union. The presence of this marking on the product indicates:

The device is not to be disposed of via the municipal waste collection system of any member state of
the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products
bearing this label, please contact your dealer or local Beckman Coulter office
for details on the take-back program that will facilitate the proper collection, treatment,
recovery, recycling and safe disposal of the device.

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1 System Description
The GS15 (Allegra 21) and GS15R (Allegra 21R) are Tabletop centrifuges designed for
laboratory use. The GS15R (Allegra 21R) is the refrigerated version and the GS15
(Allegra 21) is the non-refrigerated version. The Avanti 30 (Allegra 64R) is a refrigerated
Benchtop centrifuge. These centrifuges generate centrifugal forces required for a wide
variety of applications. They are microprocessor controlled and provide interactive
operation. The instrument design features a brushless three-phase drive system,
automatic rotor overspeed identification system, program memory that can repeat the
previously used run parameters for a given rotor, temperature control system, and a
choice of acceleration/deceleration rates. User messages alerts the operator to
conditions that may need attention.

1.1 Safety Features

Instrument safety features include:

• The door has an electromechanical door-locking mechanism to prevent operator
contact with spinning rotors. When the door is closed it locks automatically. It can be
unlocked only by pressing the OPEN DOOR key, and opened only when the power is
on and the rotor is at rest. Two independent monitoring systems prevent the door
from opening if the rotor is spinning.

• A steel barrier ring surrounds the rotor chamber to provide full operator protection.

• The overspeed system continuously monitors the rotor during centrifugation. The
system includes magnetic speed sensors in the rotor chamber and magnets
imbedded in the rotor. During programming, the system compares the rotor number
entered with the speed entered; if the set speed is greater than the rotor's maximum
permitted speed, or if the instrument detects that it is not the selected rotor, an error
code is displayed. The error must be cleared and an appropriate speed entered
before the centrifuge can be started. Throughout the run, checks are made to ensure
that the rotor does not exceed set speed.

Rotors
Make sure that only flawless rotors are permitted for use with the specific centrifuge.
Insure that the rotor chamber and seals are not damaged.

Instrument Chamber
The Instrument chamber must be clean and free of foreign particles. It is advisable when
using the refrigerated centrifuges to open the lid after centrifuging in order to allow frost
and ice to melt and vaporize.

After glass breakage, considerable contamination can occur. In this case the rotor must
be removed and carefully cleaned. Glass splinters must be removed entirely. Otherwise,

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the sealing and rotor chamber surface may be damaged. After a short period of operation
with glass splinters present a black metallic abrasive dust is produced, contaminating the
centrifuge.

Glass splinters can damage the rotor surface so heavily that the protective anodizing
layer is removed and corrosion sets in.

Rotors showing signs of corrosion may no longer be used!

In order to avoid such damage remove all traces of glass breakage. Larger splinters can
be removed by hand with special protection (gloves, tweezers, etc.). Smaller glass
particles can easily be removed by applying vacuum grease or a similar substance to a
palm-sized area and then run the centrifuge at medium speed. Afterwards wipe off the
vacuum grease with the stuck-on splinters. If required, repeat this procedure.
Check the state of the seals and grommets. Also check the boot between the drive and
rotor chamber as these are of great importance for error free centrifuge operation.

Seals also play an important part in the rotor function. An uncontrolled air flow above the
rotor could destabilize the system and cause damage.

Refrigeration performance may be severely limited by foreign air flows, which can cause
considerable frost and ice formation.

NOTE: Perform regular maintenance on seals and grommets, replace damaged seals.

Caution! Never run the drive without a rotor installed. There is the danger of the
split shaft being bent apart. Never tighten the rotor fixing screw on the shaft
without the rotor installed. The shaft would be spread and permanently deformed.

Rotors
The customer should clean the rotors once a month, or more frequently if required. After
cleaning, grease the pins of swinging bucket rotors and the shaft using a high quality
grease. (P/N 879049, 335148, etc.)
Damaged rotors may no longer be used. If in doubt, return damaged rotors to the
Indianapolis Rotor Repair Center where they are checked for possible further use.
Cracks in the material or corrosion damage always render rotors useless. Corrosion
damage is often difficult to judge, because small surface damage may continue deep into
the rotor and cause severe damage inside the material.
Make sure that the rotor coding is working, i.e. that all boreholes below the rotor contain
magnets. Missing or malfunctioning magnets may lead to the detection of an incorrect
rotor.
Sterilizing of aluminum rotors and buckets may take place in an autoclave for a maximum
of 20 minutes at max. 130°C. Higher temperatures or durations alter the physical
properties of the material and may lead to rotor breakage.

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Fixed Angle Rotors

The most frequent damage to fixed angle rotors is corrosion starting inside the
boreholes. The frequency depends on the operation mode and on the substances
centrifuged. Alkaline substances foster corrosion. Operating with a contaminated
centrifuge or high humidity in the boreholes leads to corrosion.

Swinging Bucket Rotors

The same measures and criteria for fixed angle rotors must be applied for swinging
bucket rotors. Additionally, the pivot pins for the buckets must be greased. Make
sure all four buckets are being used.

Caution: The pivot pins must be attached correctly and greased sufficiently. Their
surface must be flawless. The buckets must be able to swing out evenly and
entirely. Often uneven swinging of the buckets causes imbalance effects.

The rotor cross must also be greased, especially in the acceptance drive/ spindle hole
and at the cone. Look out for cracks, especially near the pivot pins.

NOTE: The previous information is very important to the user. Train the customer on
rotor maintenance as required.

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1.2 GS-15 / Spinchron 15 (Zent2) / Allegra 21 Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are
informative data, without guarantee.

Speed
Set speed 0 to 14500 rpm (in 100-rpm increments
or equivalent RCF
Speed display digital display indicates actual rotor speed ± 1 rpm
(actual RCF can be selected during operation)
Time
Set time to 9 hr 59 min or continuous

Time display time remaining in run (timed run)

or hold and elapsed time (continuous run)

Acceleration ten acceleration profiles

Deceleration ten deceleration profiles

Ambient temperature range 10 to 35°C

Humidity restrictions <95% (non condensing)

Dimensions
Width 42 cm (16.6 in.)
Depth 49 cm (19.3 in.)
Height, door closed 38 cm (15.0 in.)
Height, door open 80 cm (31.5 in)

Weight 50 kg (110.3 lb)

Clearances (sides and rear) 7.6 cm (3.0 in.)
Electrical requirements 60 Hz, 120 Vac, 5 A: 50 Hz, 230 Vac 3A
50/60 Hz, 100VAC, 15A
Motor 340W

Maximum heat dissipation into

room under steady-state
conditions 1160 Btu/h
Noise level 0.91 m (3 ft) <64 dBA
in front of instrument

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1.3 Avanti 30/ Allegra 64R Specification

Only values with tolerances or limits are guaranteed data. Values without tolerances are
informative data, without guarantee.

Speed
Set speed 0 to 30,000 rpm (in 100 rpm increments) or
equivalent RCF
Speed display display indicates actual rotor speed + 50 rpm
(actual RCF can be selected during operation)

Time
Set time to 9 hr 59 min or continuous
Time display time remaining in run (timed run) or hold and elapsed time (continuous run)

Temperature
Temperature setting-20 to + 40°C(in 1°C increments)
Operating range 2 to 40°C (Temperature range depends upon rotor
in use and speed,see appropriate rotor bulletin.)

Ambient temperature range10 to 35°C

For optimum operation10 to 25°C
Humidity restrictions <95% (non condensing)
Acceleration ten acceleration profiles
Deceleration ten deceleration profiles
Clearances (sides and rear) 7.6 cm (3.0in.)
Noise level 0.91m 93 ft. in front
of instrument <65 dBA

Electrical Requirements
200-V instrument 190-210 Vac, 12 A, 50 Hz
200-V instrument 190-210 Vac, 12 A, 60 Hz
208-V instrument 198-218 Vac, 12 A, 60 Hz
230-V instrument 220-240 Vac, 12 A, 50 Hz

Power cord A 1.8-m (6 ft) power cord with shock-proof plug is supplied with the instr.

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Allegra 21 Rotors

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Allegra 64R

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2 INSTALLATION

General Safety Requirements and Regulations

The user is required to take comprehensive safety measures detailed in the

corresponding operating instructions. In principle, these also apply to the service
personnel. It is obvious that not all safety precautions to be observed by users can
always be adhered to by the service personnel such as functional test with open lid.

Observe the following:

• First read the safety regulations in the operating instructions.

• Apply valid (official) accident prevention rules.
• Make sure that no safety-relevant modifications were made to the centrifuge.
• Avoid long loose hair, loose clothing and jewelry (danger of being sucked in).
• Extreme caution must be paid when performing functional test with open lid.
Apart from the obvious danger of injury caused by the moving rotor there are
secondary dangers such as breaking glass etc.
• When working at the centrifuge, unplug the main cable and take precautions
against accidental reconnection by placing a warning sigh "DO NOT CONNECT
POWER".
• When working on live components, have a second person present in order to
remove the main power in cases of emergency.
• Make sure that rotor and accessories are in flawless condition (no corrosion etc.).
• Run centrifuge only within the permitted maximum rotation speed.
• If the centrifuge density exceeds 1.2 g/cm3, reduce maximum rotation speed.

2.1 Safe Centrifuge Operation

A number of conditions must be met for the operation of a centrifuge. The safety of the
service personnel and the performance of the service tasks depend on these conditions.
The conditions are listed below.

• A secure footing of the centrifuge is ensured.

• The rotors used in the centrifuge are approved and in a flawless state (no
corrosion damage, no cracks, etc.)
• Drive shaft and cone are in flawless state; the rotor is securely fixed to the
shaft and can turn freely.
• There are no foreign particles inside the rotor chamber.
• The lid locks close properly; the lid seal is in working order.
• No flammable or volatile substances are stored in the immediate vicinity of the
centrifuge.

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2.2 Installation Requirements

Position the Instrument

WARNING: Do not place the instrument near areas containing flammable reagents
or combustible fluids. Vapors from these materials could enter the instrument's
air system and be ignited by the
motor.

WARNING: Maintain a 30-cm (1 ft) "clearance envelope" around the

centrifuge while it is running. No persons or any hazardous
materials should be within this clearance boundary while the
centrifuge is operating.

NOTE: Before attempting to move the GS-15 or Allegra 21 series centrifuge,

slide a flat implement under each of the centrifuge's rubber feet to
release the suction.

• Position the centrifuge on a level surface, such as a sturdy table or laboratory bench
that is able to support the weight of the centrifuge (refer to SPECIFICATIONS) and
resist vibration.

• Centrifuges must be located in areas with sufficient ventilation to allow for heat
dissipation.

• Check that there are 7.6 cm (3 in.) clearances at the sides and back of the instrument
to ensure sufficient air circulation.

• Ambient temperatures during operation should not be lower than 10°C (50°F) or
higher than 35°C (95°F). Relative humidity should not exceed 95% (non condensing).

• Use the anchor bolts provided to secure the centrifuge to the bench or table as
described in the publication GS-TB-016 for GS-15/ Allegra 21 and GS30-TB-003 for
Avanti 30/ Allegra 64R.

NOTE: During transport between areas with varying temperatures,

condensation may occur inside the centrifuge. Allow sufficient
drying time before running the centrifuge.

Make sure the voltage imprinted on the name rating plate affixed to the back of the
instrument agrees with the line voltage of the outlet used. Plug in both ends of the
centrifuge power cord. If there is any question about voltage, have a qualified service
person measure it under load while the drive is operating.

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WARNING: To reduce the risk of electrical shock, this equipment uses a

three-wire electrical cord and plug to connect the instrument
to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-
grounded. Check that the line voltage agrees with the voltage listed on the name
rating plate affixed to the instrument.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-

outlet receptacle strip.

NOTE: A ground lug is provided near the main plug. A separate ground wire can
be connected to the lug to ground any nonhazardous leakage current.

Test Run
NOTE: The instrument must be plugged in and the power switch
turned to on position (1) before the door can be opened.

We recommend that you make a test run to ensure that the instrument is in proper
operating condition following shipment.

2.2.1 Rotor Attachment

It is extremely important for a safe centrifuge operation that the rotor attachment to the
drive shaft is free of play. Otherwise shifts between drive and rotor can develop.
Consequences of this are bent shafts as well as damage to rotors, rotor chamber, etc.

GS15/R/ Allegra 21/R, Avanti-30/ Allegra 64R centrifuges avoid this by using a conical
seat and an additional expanding connection at the drive shaft. The conical seat is
located above the bearing plate, and the cross-slit end of the shaft with inner cone is the
expanding connection. The counterpart is the boring of the rotor, which is conical at the
bottom end.

The "collet screw" belongs to the rotor attachment. This is a screw of model-specific
dimensions equipped with a packet of spring washers and an expanding cone.
(P/N 361367)

The rotor is placed onto the drive shaft and the screw is tightened into the shaft. When
tightening the screw, the spring washers packet is compressed as far as physically
possible. The resulting tension load forces the rotor onto the cone and thus ensures that

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the bottom end of the rotor boring is free of play. When tightening the collet screw further,
the cone at the screw spreads the upper end of the cross-slit shaft apart and thus also
presses it against the upper end of the rotor boring.

The hexagonal head of the collet screw has an inside thread into which the fixing screw
of a cover can be affixed.

Caution: First safely attach the rotor using the collet screw: then
screw on the lid.

Make sure the drive shaft and rotor boring are free of dirt and foreign particles. To
prevent the rotor from sticking to the shaft, swab the inside of the rotor drive hole with
Spincote or equivalent product. (P/N 961660, 306812, etc.)

Also make sure that the collet screw is not tightened into the shaft with no rotor installed.
The spreading cone would spread the shaft too far and deform it permanently. The rotor
would afterwards jam on the shaft and thus not be pressed onto the cone with the
required force. If, however, the permanent deformation has occurred, the ends of the slit
shaft can be carefully bent back into place using smooth, isolated pliers. Be very careful
not to damage the shaft surface. Replace the drive if the damage cannot be repaired.

Be sure to inform the user of the importance of a secure seating of the rotor and the
principle of the attachment.

Caution: Warn the user against possible handling errors and

their consequences!

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2.2.2 Customer Training and Instrument Demo

The following check list is useful when customer training is required.

The Allegra 64R requires installation by Beckman Coulter Field Service personnel.

Prior to installation, contact the customer to ensure electrical requirements have been
completed (GS-30TB-008A). Adjust your, training to the customer's needs.

Refer to User's Manual for all of the following:

Explain:

Rotors
Warranty
Maintenance
Responsibility
Cautions

Descriptions of instrument
Functions
Controls
Indicators
Rotor chamber
Temperatures sensing and control
Drive
Safety features
Name rating plate, serial number

Run the instrument

Help the customer do a simple run.

Explain:

• Troubleshooting
• Customer maintenance items
• Beckman Coulter Field Service, service agreements, etc.
• Accessories and options that are available for the instrument
• How to get applications support from Beckman Coulter

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THIS PAGE IS INTENTIONALLY BLANK

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3 Service Procedures
3.1 GS-15, Allegra 21, Spinchron 15R Service Procedures

3.1.1 Door Latch

3.1.2 Emergency Release of the Door Latch

It may be required to manually release the door latch.

See GS-15/R, Allegra 21/R, Spin 15/R, Instruction Manuals for Emergency access
Procedures.

Caution! Release locks only if the rotor is stopped and no danger to another
person exists.

Insert 4 mm (5/32”)
Allen wrench and
turn to release lid

3.1.3 Latch Replacement

Remove front cover.
Remove connector and ground wire from the bottom part of the lock.
Unscrew and remove 2 fixing screws at the side of the latch.
Remove the string for mechanical latch release.
Replace latch.
Reinstallation proceeds in
reverse order.

Welded
Caution: Never run centrifuge Stud
with unsecured open door.

Standoff

Latch
Screw

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3.1.4 Drive Assembly

The drive is located at the geometric center of the rotor chamber. Drives are taken out
upward through the rotor chamber and also reinstalled from the top. The respective rotor
is attached to the drive shaft in the rotor chamber.
The drive comprises vibration dampers and a rubber boot forming the motor suspension.
The motor itself is suspended in an oscillatory manner. A rubber boot encloses the motor
and seals the armored chamber bottom. The rubber boot is attached to the motor via a
round cover plate.
Protection against operation at a too high imbalance is ensured by the imbalance switch
located at the bottom of the motor.

3.1.5 Drive Suspension

Rubber Boot
In all models, there is a rubber boot at the top of the motor. It is attached to the motor
with a cover plate. The rubber boot seals the rotor chamber against the motor. It is
positioned into the opening in the bottom of the rotor chamber. The connector cable for
the rotor detector runs through the rubber boot.

Vibration Mounting System

Centrifuges are operated at more or less significant imbalances. The drive must be
suspended in order to allow the rotor to rotate around its axis and through its center of
gravity, instead of around its geometrical axis. The drive attached to the rotor is thus
suspended with elastic elements (rubber mounts).
This constitutes a physical spring-mass system, which oscillates in itself. This oscillation
tendency depends on the elasticity constant and mass properties; it can be so strong that
the centrifuge becomes unstable and insufficient dampening could lead to destruction.
Such dangerous operation states can be avoided using vertical and horizontal vibration
dampers. Special care must be taken with respect to the dampers during maintenance.

Rubber Vibration Mounts

Rubber mounts consist of a stable rubber piece with a sheet metal yoke and a threaded
pin at either end. The metal parts are greased with silicone oil in order to reduce friction
within the yokes. Inspect the oil film during maintenance and repair. Exchange cracked
rubber mounts. Take care that the rubber mounts are not twisted during installation.

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Replace as Assembly

Metal Brackets
on both ends

Black
Rubber

Rubber Vibration Mount-Removal

For removal of Rubber Vibration Mounts proceed as follows:

- Loosen fixing nuts at the bottom of the chamber.
- Remove 2 hexagonal screws of the damper (1).
- Take out rubber damper from support angle.
- Reinstallation proceeds in the reverse order.

Suspension Maintenance
- Check the listed components visually:
- Check rubber components for cracks and holes; replace if necessary.

3.1.6 Drive Removal

Caution! Prior to start of removal always disconnect the main power

plug and prevent accidental reconnecting by placing a warning sign:

"DO NOT CONNECT TO POWER!"

Proceed as follows:
- Remove covers (front & rear) and open lid.
- Loosen rotor tie down nut and remove rotor.
- Turn instrument on its right side and unscrew round bottom cover.
- Unplug connector cables from the tachometer board.
- Unplug drive cable from the power board.
- Remove power board.
- Unscrew 4 screws from the motor cover inside the rotor chamber.
- Remove motor cover with rotor ID device (PN 365752). Do not damage
cable and plug in the process!
- Remove rubber boot (PN 364206).

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- Unscrew 3 fixing screws of the tachometer board support angle (A).

- Remove support angle with tachometer board.
- Remove screw (B).
- Remove motor through the chamber.

Tach sensor board attached at the bottom of the drive

3.1.7 GS15/R, Allegra 21/R, Spin 15/R Imbalance

These Models use a mechanical imbalance switch. It is located at a support
bracket beneath the motor.
In case of an imbalance the mechanical imbalance switch is pressed against the
imbalance switch plate.

Contact
Gap
Mech. Imbalance
Fig. 1 Fig.2

For removal proceed as follows:

Unplug connection cable to the power board.
Unscrew 2 fixing screws of the support angle (Fig.1, item 1).
Unscrew 2 fixing screws of the imbalance switch at the support angle (Fig1, item2).
Replace imbalance switch.
For reinstallation proceed in reverse order.

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After installation adjust the contact gap as follows.

Loosen slit screw at the contact (Fig.2, item1).
Slide the contact at the rear of the imbalance switch in such a way that the contact gap is
1.0 + 0.1mm (Fig.2, item 2).
Tighten slit screw.
After installation operate the centrifuge with an imbalance of 6 grams. It should not fail.
Use decel value of “3” when testing the imbalance at 6 grams.

With a swinging bucket rotor the imbalance switch must fail at 15 grams imbalance. The
imbalance LED will come on and the drive will brake fast. Otherwise repeat the
adjustment. The centrifuge should operate with up to 6 grams of imbalance and not fail.
With a fixed angle rotor it should pass at 10 grams and fail at 20 grams.

Note: 1 milliliter of water equals 1 gram.

3.1.8 Cooling
The heat generated by the centrifuge operation requires cooling of the instrument. Two
methods are used, air cooling and refrigeration cooling.

Air Cooling
An air flow must pass through the rotor chamber in order to dispose of the heat
generated by the rotor. For this purpose, the door is not hermetically sealed as in the
refrigerated centrifuges. The air-cooled centrifuge is designed to allow air flow through
the system.
The room air used for cooling is drawn in at the rear of the door and exits through the
center of the door openings into the rotor chamber and past the rotor. The air flow is then
directed over the top edge of the rotor chamber and continues outside through the slit
between rotor chamber and armored chamber downward. Via gaps in the lower
intermediate ring the cooling air flows across the drive and cools its surface.
Temperatures below ambient are thus unobtainable, as opposed to temperatures above
ambient. The pressure gradient necessary for generating the air flow originates from the
rotor movement.

Air Cooling-Air Flow schematics

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3.1.9 Refrigeration Cooling

In laboratory centrifuges, the drive energy (apart from acceleration and deceleration
phases) is converted to heat. In order to prevent the rotor from excessive heating, this
heat must be disposed of in a suitable manner. This can be done with an air flow through
the rotor chamber (as for the air cooled centrifuges). If temperatures below the ambient
are required, refrigeration must be used.

Refrigeration Compressor
The compressor is the core of the refrigeration system.The compressor draws off the
cooling gas charged with the rotor heat energy from the evaporator (cooling coil around
the rotor chamber) and compresses it.
During this compression the heat contained in the cooling gas is "elevated" to a higher
temperature (compression heat). Only hermetically sealed and maintenance-free
compressors are used. Maintenance of these compressors is confined to occasional
cleaning of the surface and a check of the elastic mounts. The electrical equipment of the
compressor consists of a startup capacitor and also a run capacitor.
A start-up relay is located inside the clamping box. The relay switches on an additional
auxiliary winding in the compressor as an aid for starting up. The R-C circuit also located
inside the clamping box limits the inductive voltage peaks caused by the switching.
The start-up relay as well as the R-C circuit may fail and must therefore be checked
during maintenance.

Refrigeration Compressor

Clamp Box

Inside the clamping box of the compressor, the circuit must be configured according to
the circuit diagram below.
The clamp box is fixed to the compressor by means of a clamp. Remove the clamp using
a pointed tool such as a screwdriver.

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Condenser
The hot cooling agent gas is compressed by the compressor and then fed into the
condenser. The heat energy contained within is transferred to the ambient air by means
of a mechanically generated air flow. Thus the gas is cooled and fluidized (condensed).
The condenser consists of a coiled tube inside a finned body. A fan is installed behind
the condenser, drawing in cool ambient air through the grill (finned body) of the
condenser. Dust from the ambient air can be caught in the finned body of the condenser,
blocking the heat transfer to the cooling air and infringing on the performance of the
cooling device. It is necessary to clean the grill (fins) at regular intervals (at least on the
occasion of the annual maintenance). Use a brush or compressed air for cleaning.
Wash out greasy and sticky dust layers using suitable detergents. The grill can be bent
by mechanical force. The air flow can be blocked by parts of the grill being bent. When
this happens realign the grill. Realign the grill using slim adjustment (needle nose) pliers.
The grill spacing is 5 fins per 1 cm. (12 fins per inch)

Expansion Device
Expansion valves or capillary tubes are used as expansion devices. In principle, this is a
throttling section, where the pressure gradient generated by the compressor is
decreased, and the fluid cooling agent expands. Capillary tube injections are used in this
case. The cooling agent existing from the expansion device into the evaporator is called
"injection". The capillary tube may become clogged due to impurities in the cooling
circuit. In this case replace the capillary tube.

Evaporator
After the cooling agent has left the expansion device, it is injected into the vaporizer,
expands and changes its state of aggregation by vaporizing. Heat is extracted from the
ambient (in this case the rotor chamber) during this procedure; thus the rotor chamber is
cooled. At the evaporator output the cooling gas loaded with the heat energy is drawn in
again by the compressor.

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The evaporator consists of a copper tube wound around the rotor chamber in tight turns
and soldered to the chamber for better heat conductivity. The evaporator is thermally
shielded against the ambient air by means of an insulation layer. Therefore only the heat
generated in the chamber is reduced.

3.1.10 Servicing the Refrigeration System

Cooling circuit repairs may only be performed by a trained refrigeration technician.

Filling Capacities: ( 1 gram = 0.03527 ounce)

In order to judge the operation of a refrigeration device, in case of a malfunction, a
refrigeration technician can measure the pressure inside the system using a refrigeration
manometer. The table below lists the cooling agent filling capacities.

R134a GS15R = 185 gram or 6.5 ounce R134a All21R =185 gram or 6.5 ounce

3.1.11 Cooling System Performance

In a refrigerated centrifuge the heat caused by air friction is eliminated by using cooling.
The temperature is measured inside the rotor chamber and then regulated to the value
preset by the user via the microprocessor.
This mechanism can only work as long as the compressor can cope with the heat to be
eliminated.

Compressor performance is determined by its capacity and influenced by outer

conditions. High ambient temperature or contamination of the cooling refrigerant
can reduce the performance significantly.
Compressor performance depends on the temperature range in which it is functioning. At
lower centrifugation temperatures less heat has to be eliminated than at higher
temperatures.
The amount of heat to be eliminated from the rotor chamber is determined by the rotors
used, by the operation speed, and the preset temperature be reached.

However, if lower temperatures than the obtainable ones are required, reduce the
rotational speed. No general rules or values for the reduction of maximum speeds
can be given. Determine experimentally, at which speed the desired lower
temperature is obtained.

The maximum rotational speed may only be reduced as far as to ensure that the air flow
generated by the rotor is still sufficient to transport the heat energy from the rotor to the
cooler rotor chamber. This can be especially true for fixed angle rotors.

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3.1.12 Calibration of the Refrigeration System

Test the Charge

After charging the system you should check the performance to insure proper charge.

Check temperature offset in the service mode

(This is to test the charge)

Turn the centrifuge on, but no rotor turning. (0 RPM)

Set temperature to -20°C.
Wait 1 hour.
After 1 hour the indicated temperature should be -2°C or lower. If not -2°C or lower adjust
the refrigerant charge. (i.e. add more refrigerant)
Next set speed to 4,800 RPM, time hold and temperature to 5°C.
The temperature should be able to reach 5°C.

Test for pressure while under heat load.

CAUTION: Cover backside of power receptacle (P/N964643) with electrical tape

when servicing the rear of the instrument to protect against shock hazard.

To check the filling capacity with a refrigeration manifold gauge set, proceed as
follows:

Ambient temperature should be 22-27°C.

Put low side gauge on service value of refrigeration compressor.
Set for maximum cooling -20°C
Set rotor for maximum speed, set time for hold.
Run rotor at maximum speed.
After 30 minutes the low side gauge should read: 5 psig, R134a
With instrument off (unplugged), after 30 minutes the gauge should read 35-45 psig.

Temperature Sensor
Refrigerated centrifuges are equipped with a temperature sensor for measuring the
temperature, located inside at the bottom of the centrifuge. A thermistor sensor is used.
In case of malfunction the sensor (which is poured into a metal jacket and fixed to the
bottom plate of the centrifuge) must be replaced. Malfunctions of the wiring and the
connectors are also possible and must be repaired. After the circuit board is exchanged
take care to correct the offset values in the EEPROM. To replace the Rotor Chamber
Temperature Sensor, first remove the RTV from the bottom (outside) of chamber bowl.
Then the sensor is pressed down from the inside of the chamber out through the bottom
in a GS15, Allegra 21, Spin 15.

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Temperature sensor P/N 365757

Remove sensor by pressing down after removing RTV from bottom

GS15R, Allegra 21R, Spin 15R

3.1.13 Chamber Gasket Replacement GS15, Allegra 21, Spin 15

A rubber sealing ring is located at the top edge of the chamber. Replacement is
required if the sealing is damaged or gets brittle.
Press defective sealing inward towards the central chamber axis.
Pull off the sealing ring from the chamber edge towards the center and remove it.
Apply silicone (RTV P/N 342778) cement to the groove of the new sealing.
Starting with the seam, place the new sealing onto the chamber edge.
Smooth the remaining bulge outward using a rubber mallet.
Wipe off the excess cement.

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3.1.14 Electronics

Electronic Tests
For checking the Tach sensor and the rotor ID sensor the rotor can be rotated
by hand (with open lid). The speed sensor LED must light-up six times per revolution.
The rotor detection LED must light up according to the rotor code, where one coding
magnet switches it off again. Spin the rotor by hand and confirm the LED lights up.

Tach Sensor
The Tach sensor consists of a bistable Hall sensor which is placed in a small plastic
casing along with a small circuit board and passive components, to protect it against
shock and humidity. This unit can not be repaired and must be replaced as a whole.
A magnet segment with 6 pairs of poles rotates 1+ 0.5 mm close to the sensor. A
northpole in front of the Hall sensor switches the output to H = 5 V, whereas a
south pole switches to L = O V. Therefore 6 H/L transfers must occur per rotor revolution.
This is 3 highs and 3 lows.
The plastic casing enclosing the Hall sensor together with two plug connections is
mounted on a small circuit board located beneath the motor. One plug connection leads
to the power board. Ensure the connector position is marked before unplugging.

For removal proceed as follows:

Place centrifuge in such a way that the bottom is accessible from the outside.
Remove bottom plate (if required).
Unplug cables.
Unscrew 3 support angle screws from the motor (1).
Remove support angle with tachometer board and replace it if required.
Reinstallation proceeds in reverse order.

Tach sensor

Rotor Identification
The same Hall sensor as described previously is used. It is mounted inside the rotor
chamber on the motor cover.
Small bar magnets of changing polarity are located in the rotor at a distance of
0.8...2mm.
Between 2 and 12 magnets are located on a circle that is divided in 30° sectors. The
number is always even. Angular position and number of magnets form the rotor code.

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For removal proceed as follows:

Unscrew the 4 fixing screws of the rotor cover (1).
Lift cover with mounted sensor carefully.
Unplug the connector on the Display Control Board.
Replace Rotor Id Plate.
For reinstallation proceed in reverse order.

Rotor ID sensor

Display Control Board

The Display control board is located behind the front cover attached to the Touch Panel.

Caution! Prior to start of work always disconnect the main plug and prevent
accidental reconnecting by placing a warning sign

"DO NOT CONNECT TO POWER".

NOTE: The micro controller board contains static-sensitive CMOS components

(i.e. damage may be caused by touching with your fingers). "Use anti static
mat, grounding straps and other anti static devices."

Power Board

Position, Removal and Installation

It is located in the front on the bottom base plate.
The power board must rest on a clean, anti static surface during maintenance.
Mark and then disconnect all connectors from the power board.
Use a Phillips screwdriver and remove only the front screws, lift the heatsink and pull it
out of its position. The back screws are loctited in place as there are cutouts on the
heatsink. The brake resistor is attached to the heatsink and need caution when pulling
the board out of the instrument.
Remove and replace the powerboard as a whole Assembly.
For reinstallation proceed in reverse order.

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Power Board Layout GS 15./R, Spin 15/R, Allegra 21

Powerboard Zent 2 1996 - Today

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3.2 Avanti 30, Allegra 64R

3.2.1 Door latch

3.2.1.1 Door Latch Replacement

Remove front covers.
It requires a 2.5 mm Allen and 7mm open end wrench.
Remove connectors from the bottom part of the lock.
Unscrew and remove 2 fixing screws that attach the latch to the housing.
Replace latch.
Reinstallation proceeds in reverse order

3.2.2 Drive Assembly

3.2.2.1 Drive Suspension

The motor is suspended via 5 oil dampers providing vertical and horizontal stiffness and
dampening. The motor also has a rubber boot to seal the rotor chamber. The following
sections explain these.

Oil Vibration damper

Oil Vibration Dampers
Model Avanti 30, Allegra 64R drives are equipped with oil vibration dampers. They are
attached to the drive base plate with one hex nut and to the chassis with a plastic clamp.
Replace the oil vibration dampers if one of the following occurs:
- Vibration damper piston is not covered with oil.
- External oil traces are visual.
- Rubber parts become cracked.
For replacing proceed as follows:
- Pull damper out of plastic clamp.
- Loosen hex nut.
- Unscrew rubber buffer.
Reinstallation proceeds in reverse order.
Make sure to tighten counter nut!

NOTE: The vertical oil vibration damper contains thin oil, the horizontal oil
vibration damper contains viscous oil and is marked with a 3. Because of their
similar appearance DO NOT interchange.

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Avanti 30, Allegra 64 Drive Assembly

Motor Removal
Avanti-30, Allegra 64R drive Assy (P/N-364230) is removed & reinstalled as a complete
Assembly with 3 oil dampers attached. Care must be given not to damage the dampers
while handling. Keep the dampers from pulling out and coming apart.
Loosen the imbalance sensor bracket and move it all the way back. Be careful not to
damage it while removing the motor.
Insure all drive cables are disconnected and won’t interfere during removal.
Disconnect the drive mounts from the chassis carefully.
Tip the instrument to remove the drive only to the right side to avoid problems with the
compressor when turning back on.
Lower the motor down through the access hole and out.
Reinstallation proceeds in reverse order.

3.2.3 Gray Wedge Filter Imbalance Sensor (Avanti 30, Allegra 64R only)
Avanti 30, Allegra 64R is equipped with an imbalance sensor in the shape of a gray
wedge filter, oscillating within a light barrier to monitor for imbalance.

Description of the optical imbalance detector (gray wedge filter):

A sensor with a transparency gradient (called gray wedge filter in the following) oscillates
within a light barrier according to the motor amplitude. An electronic circuit measures the
brightness variations and thus the amplitude. If a permitted limit is exceeded, the
centrifuge switches off with the imbalance lighting up.

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Replacing the Gray Wedge Filter foil (Zent)

To replace the gray wedge foil, loosen the bracket at the bottom of the motor, remove the
foil and install the new foil be oriented with the shading decreasing going from bottom to
top.
• Activate the service mode #5:
• Press Cursor Up key once,
• Press Enter three times, holding down the key the third time until the Service
• Mode appears in the time display = ‘Ser’
• Press Cursor key and scroll to #5
• Press ENTER to activate.
•
The static imbalance setting is indicated service mode 5.

The time and speed fields display the following parameters:

time = ‘STA’
speed = DC output of the imbalance sensor, nominally 200 without a rotor when
properly adjusted.
As the imbalance sensor board or foil is moved up, this value increases, moved down,
this value decreases. The film should be positioned far enough inside the sensor arms,
>0.2”, to cross the optical path (0.1” from the opening.)

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3.2.3.1 Imbalance Adjustment Instructions (Avanti 30, Allegra 64R)

Load fixed angle rotor with a gross switch-off imbalance weight.

Shut-off Weights Fail Pass

F0685 25 5
F1202 10 2
All Other Fixed Angle Rotors 25 5

Activate the service mode:

Press Cursor Up key once,
Press Enter three times, holding down the key the third time until the Service
Mode appears in the time display = ‘Ser’
Use Arrow keys and select Service Mode #2
In service mode #2 an imbalance correction value can be set to increase or decrease
the sensitivity to an imbalance. A positive value decreases and a negative value
increases the sensitivity.

The correction value is valid for all rotors!

The following table shows the conversion of correction values into their
corresponding correction factor. The correction value has to be entered in the
service mode. The correction factor is calculated as follows (see table):

Correction value Correction factor

-30 1/(1+3.0)=0.25
-20 1/(1+2.0)=0.33
-10 1/(1+1.0)=0.5
0 1+0.0=1
10 1+1.0=2
20 1+2.0=3
30 1+3.0=4

The correction value should be determined by means of a parameter setting run.

To do this, proceed as follows:
• Activate the service mode and select service mode 2
• Press ENTER until the imbalance LED is ON
• Quit the service mode by pressing STOP
• Load rotor with test weight
• Select maximum speed
• Set temperature to 40C (OFF)
• Set time to HOLD
• Press START
Rotor accelerates to the maximum speed
Run is stopped automatically

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After rotor has come to rest, the imbalance LED turns OFF
Correction value stored in EEPROM

• Carry out a test run with cut-off weights to verify the correction value has been
established correctly.

The value can also be manually set in the service mode by programming a value
altered in the right direction: positive (less sensitive) and negative (more
sensitive):
• Activate the service mode and select service mode 2
• Press ENTER once to activate the manual input mode
• Measured correction value flashes in the temperature display
• Set desired correction value by using the ↓and ↑keys
• Confirm and leave service mode by pressing STOP

Calibration of the R404a Refrigeration System

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Temperature sensor P/N 961801

3.2.3.2 Remove sensor by pulling after removing RTV

R404a Avanti 30 = 256 grams or 9.0 ounce

R404a Allegra 64 =256 grams or 9.0 ounce

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3.3 Service Mode Zent Version Electronics

To activate the service mode:

• Press Cursor Up key
• Press “Enter” three times, holding down the key the third time until the
ServiceMode appears in the time display = ‘Ser’
The program and speed fields display the following parameters:

program speed description

0 ver software version and centrifuge type
1 sen sensor status
2 i-fak imbalance factor
3 error error table
4 eep-- erase EEPROM or Door Lockout Timer
5 imbs static imbalance
6 imbf dynamic imbalance
7 led88 LED test
8 zeit accumulated run time
9 zykl cycle mode
10 offse temperature offset

Select the program using the ↑and ↓keys.

To exit any service program press STOP.

#0 Software Version and Centrifuge Type

Press ENTER to activate.
The time and speed fields display the following parameters:
time = Software Version
speed = Centrifuge Type

#1 Sensor Status
Press ENTER to activate.
The program, time and speed fields display the following parameters:
ACC/DEC field =
digit 1, i.e. left = 1 inverter bus charged, 0 inverter bus discharged
digit 2, = 1 inverter enabled, 0 inverter not enabled
TIME field =
digit 1, i.e. left =1 door closed, 0 door not closed
digit 2 = n/a
digit 3 = 1 chamber over temperature (O/T), 0 no chamber O/T
SPEED field =
digit 1, i.e. left = 1 no inverter O/T, 0 inverter O/T
digit 2 = 1 imbalance, 0 no imbalance
digit 3 = 1/0 rotor ID sensor output
digit 4 = 1/0 tach sensor output
digit 5 = 1 rotor turning, 0 rotor stopped at least 2 seconds

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#2 Imbalance Test (Avanti 30, Allegra 64R only)

In service mode 2 an imbalance correction value can be set to increase or decrease
the sensitivity to an imbalance. A positive value decreases and a negative value
increases the sensitivity. The factory tests for the imbalance threshold using the
F0685 with filled tubes and -15g imbalance.

The correction value is valid for all rotors of one centrifuge type!
The test mode is indicated with the imbalance LED constantly ON.
Activate by pressing ENTER and exit the service mode by pressing STOP.
Proceed with a test run.
When finished testing, enter the Service Mode, select program 2, and deactivate
by pressing ENTER.
The following table shows the conversion of correction values into their
corresponding correction factor. The correction value has to be entered in the
service mode. The correction factor is calculated as follows (see table):

Correction value Correction factor

-30 1/(1+3.0)=0.25
-20 1/(1+2.0)=0.33
-10 1/(1+1.0)=0.5
0 1+0.0=1
10 1+1.0=2
20 1+2.0=3
30 1+3.0=4

The correction value should be determined by means of a parameter setting run.

To do this, proceed as follows:
• Load rotor with test weight
• Select maximum speed for the selected rotor
• Set temperature to 40C (OFF)
• Set time to HOLD
• Activate the service mode and select service mode 2
• Press ENTER (repeatedly if required) until the imbalance LED is ON
• Quit the service mode by pressing STOP once. Imbalance LED should remain on.
• Press START

Rotor accelerates to the maximum speed

Run is stopped automatically
After rotor has come to rest, the imbalance LED turns OFF
Correction value stored in EEPROM
• Carry out a test run with cut-off weights to verify the correction value that has been
determined.

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The value can also be manually set in the service mode by programming a value
altered in the right direction: positive (less sensitive) and negative (more sensitive):
• Activate the service mode and select service mode 2
• Press ENTER once to activate the manual input mode
• Measured correction value flashes in the temperature display
• Set desired correction value by using the ↑and ↓keys
• Confirm and leave service mode by pressing STOP

#3 Error Table
Press ENTER to activate.
The 14 most recent errors are stored in the error table. Pressing the ↑and ↓keys
scrolls through the list of errors. Pressing RPM deletes the error from the list.
The time and speed fields display the following parameters:
TIME field = sequence number
SPEED field = error number

#4 Erase EEPROM or Door Lockout Timer

The speed field displays ‘ERASE’. Two options are available by pressing the
following keys:
ENTER = deletes the error table; exits Service Mode
not effected:
elapsed run time, cycle counter, temperature offset, imbalance thresholds.
RPM = deletes errors (28), run time, temperature offset, imbalance thresholds,
and overrides Door Lockout Timer, not effected:
elapsed run time, cycle counter; exits Service Mode.
The accumulated run time and cycle counter values can be deleted in programs 8 and 9,
respectively. The temperature offset and imbalance thresholds revert to
their default values.

#5 Static Imbalance Mode (Avanti 30, Allegra 64R only)

Press ENTER to activate.
The time and speed fields display the following parameters:
TIME field = ‘STA’
SPEED field = DC output of the imbalance sensor, nominally 200 without a rotor when
properly adjusted.
As the imbalance sensor board is moved up, this value increases. Moved down, this
value decreases. The film should be positioned far enough inside the sensor arms, >0.2”,
to cross the optical path (0.1” from the opening.) The film should be oriented with the
shading decreasing going from bottom to top
Pressing STOP exits the program.

#6 Dynamic Imbalance Mode (Avanti 30, Allegra 64R only)

Press ENTER to activate.
The time and speed fields display the following parameters:
TIME field= ‘DYN’
SPEED field = AC output of the imbalance sensor, normally between 900 and 1100

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Press ENTER to deactivate.

#7 LED test
Press ENTER to activate.
Every discrete LED and seven segment LED digit is turned ON.
Pressing STOP exits the program.

#8 Accumulated Run Time

Press ENTER to activate.
The speed and time fields display the following parameters:
SPEED field = TIME field = hhh & hh-mm
A maximum of 99999 hours and 59 minutes can be displayed, about 11 years.
Pressing RPM clears the stored elapsed time in the EEPROM.
Pressing STOP or ENTER exits the program.

#9 Cycle Mode
Press ENTER to activate.
The centrifuge can be programmed to cycle automatically. The time and speed
fields display the following parameters:
TIME filed = time between cycles (minutes)
SPEED field = cycle counter
The following keys are used to program the cycle mode parameters:
↑and ↓= time between cycles (1minute increments)
RPM = clear the cycle counter
START = begin cycling and start the centrifuge
FAST STOP = end cycling and stop the centrifuge
STOP or ENTER = exits the program.

#10 Temperature Offset Mode

Press ENTER to activate.
A temperature offset can be programmed to be added or subtracted from the
temperature display to match the temperature measured with a dunk test.
The speed, time, and temp fields display the following parameters:
SPEED field = temperature including offset
TIME field= temperature offset
TEMP field= temperature without offset
↑and ↓= offset (+/- 0.1°C increments)
STOP or ENTER = exits the program.

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Error list for Zent Version Electronics

GS-15/R, Allegra 21/R, Spinchron 15/R (Z2) - Avanti 30, Allegra 64R (Z1)
No. Z1 Z2 Type Description Service Procedure
System CPU:
1 X X fail CPU test after power replace EPROM,
up failure replace Control Board
2 X X stack stack overflow same as error #1
3 X X CPU RAM failure same as error #1
4 X X RAM external RAM failure same as error #1
5 X X EPROM Checksum invalid same as error #1
6 X X Comm. Communication fault same as error #1
7 X X Comm. Keypad to Display replace Keypad and/or Display
connection Board
communication error
8 X X Watchdog Frequent tipping + 5Volt power supply on control
board, replace EPROM, replace
control board
9 X X status SW traps Replace EPROM, replace control
disallowed board
10 N/A
Motor CPU
11 X fail CPU test after power Replace EPROM, replace control
up failure board
12 X Stack Stack overflow same as error #11
13 X CPU RAM CPU RAM failure same as error #11
14 X RAM external RAM failure same as error #11
15 X EPROM Checksum invalid same as error #11
16 X Comm. Communication fault same as error #11
17 X Reset Motor CPU reset same as error #11
18 X Watchdog Frequent tripping + 5 Volt power supply on
Control board , replace EPROM,
replace control board.
19 X Status Disallowed SW straps Replace EPROM, replace Control
board
Tach System,
CPU
20 X X Unstable Interrupt faulty Tach sensor to Control board
connection, Tach sensor cable
grounding, Tach sensor/magnet
ring gap, replace tach sensor
21 X X Overspeed RPM exceeds limit Same as error #20
22 X X unstable Noisy or jittery Same as error #20
23 X X Compare Inconsistent reading Same as error #20
between motor and
system CPU
24 X X Control Final speed not AC input voltage, how easily the
reached rotor turns, replace power board,
replace drive
25 X X Drive Inverter problem AC input voltage, replace Power
board
26 X X Monoflop No indication of rotor Replace EPROM, replace Control
turning even though board
tach is ok

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No. Z1 Z2 Type Description Service Procedure

27 X X PWM Check PWM signal or same as error #26
feedback signal absent
28 X X No Tach Start without Tach Same as Error #20 Door lock
requires power to be left on for 12-
15 min., or can be cleared with
serv. Mode #4, replace
Powerboard, latch
29 X X No tach Loose tach during run Same as error #28
30 X Compare Inconsistent over Same as error #26
speed limit between
motor and system
CPUs
Tach, Motor
CPU
31 X Overspeed RPM exceeds limit same as error #20
32 X Unstable Noisy or jittery same as error #20
33 X Compare Inconsistent tach same as error #20
reading between motor
and system CPU
34 X Control Final speed not same as error #24
reached
35 X Drive Inverter problem same as error #25
36 X Monoflop No indication of rotor Replace EPROM, replace Control
turning even though board
tach ok.
37 X PWM Check PWM signal or same as error #26
feedback signal absent
38 X No rotor Start without rotor same as error #20
39 X No tach No tach indication same as error #20
40 X X Overspeed Hardware overspeed Overspeed jumper settings,
test failure replace control board
41 X X Overspeed Hardware overspeed Same as error 40, check Tach
detection , at max rotor cable routing
speed
42 X X Imbalance Hardware imbalance Imbalance sensor and Control
test failure board connection, imbalance
sensor position, replace sensor
43 X X Imbalance DC offset not in range same as error #42
50 X X Status Floating point operation Replace EPROM, replace Control
error, overflow board
51 X X Status Floating point operation same as error #50
error overflow
52 X X Status Floating point operation same as error #50
error, underflow

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No. Z1 Z2 Type Description Service Procedure

EEPROM:
69 X X Type Not compatible EEPROM loose, same as error
#20
70 X X Type Version not compatible Same as Error #69
with SW version
71 X X Hardware Not responding Same as Error #69
72 X X Verify Not responding Same as Error #69
73 X X Checksum Program data not valid Same as Error #69
74 X X Checksum Free area not valid Same as Error #69
75 X X Checksum Imbalance not valid Same as Error #69
76 X X Checksum Offset not valid Same as Error #69
77 X X Checksum Program variables not Same as Error #69
valid
Door:
80 X X Transistor Transistor failure Replace power board
81 X X Monoflop Rotor turning with door Turning rotor by hand with the
open door open, replace door latch,
tach sensor
82 X X Test Door lock test signal Door latch to power board
failure connection, replace power board,
replace control board
83 X X Open failure Door won’t open Replace latch, same as error #82
84 X X OT Chamber O/T Refrigeration system including
power board, relay, O/T sensor
85 X X O/T Rotor exceeds 50°C Refrigeration system including
power board, relay
87 X X Temperature Temperature signal out Temperature sensor to control
of range board connection, replace temp.
sensor replace control board
88 X X O/T Motor exceeds 70°C Airflow, O/T sensor and cable
90 X X Temperature Temperature sensor Same as error #87
failure
94 X X Imbalance Static imbalance Same as error #42
sensor failure
95 X X Imbalance Dynamic imbalance Replace EEPROM. Replace
sensor failure control board
98 X X Rotor ID Invalid Rotor ID Rotor ID sensor to rotor gap, rotor
missing magnets, rotor ID sensor
or cable
99 X X Rotor ID Invalid rotor ID Wrong rotor ID keyed in by User
same as error #98

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THIS PAGE IS INTENTIONALLY BLANK

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4 GS-15/R/ Allegra 21/R, Spinchron 15R, Avanti 30/ Allegra 64R

CIRCUIT DESCRIPTION
4.1 Circuit Description Block Diagrams Zent 2 Version Electronics
The block diagram below shows the processor with its memory components and
the communications periphery.

DisplayElectronics
Control w i th RA M
Drivers
Display with RAM
Drivers
Micro-
processor
Shift Register EPROM

Microprocess
or
S h i ft R e g i s te r
Keyboard EPR O M
EEPROM

EEPR O M
Keyboard
Frequency
Converter

Lid F r eque nc y
Lock
ConveMotor
r t er

M o to r
Tach Generator

Power Electronics with Motor

Micro Controller Board - Block Diagram

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4.1.2 Power Board

The power board comprises relay operation control, power supply for the micro controller
board, intermediate circuit, the star-delta switch proper, brake chopper, over voltage
monitoring, transistor temperature monitoring, and possibly the imbalance switch.

4.1.3 Electronic Speed Regulations for Centrifuge Drives

All laboratory centrifuges require drive systems with variable rotational speed. Universal
motors were used for this purpose, where the commutation takes place by carbon
brushes and collectors. By means of a speed control, a specific rotational speed could be
adjusted and regulated within an adjustment range. Sometimes regulating transformers
were used as speed adjustment circuits.
Universal motors have system-inherent disadvantages. For example, brushes and
commutators wear out quickly causing frequent maintenance and shortening the life. The
brush dust causes contamination, interfering with laboratory work. Undesired carbon
dust deposits in the centrifuge may occur.
An alternative to the universal motor is the three-phase asynchronous motor,
characterized by its simple structure. In the industrial drive technology for fixed speeds it
is by far the motor type most widely used. If driven with AC current they provide high
torque and are less noisy. With brushes no longer in use, the only life cycle-significant
elements are the bearings. This is of secondary importance. The asynchronous motor
can be regarded as maintenance free.
In order to operate an asynchronous motor at varying speeds it must be driven with
three-phase current of variable current or variable frequency. For this purpose a three-
phase converter is required, driven by a DC voltage source (the intermediate circuit).
The power transistors are digital switches, because they can only be switched on or off.
The speed sensor is also digital. Thus the entire speed regulation system can be
designed digitally if a microprocessor is used. As compared to analog systems the
advantage is greater stability and simple integration. Adjustment is not required.
Remaining functions the microprocessor cannot handle due to its limited operation
speed, are integrated into its own gate array. The result is a system with economic
component use and high flexibility.

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Intermediate Circuit (Bus Current)

For the intermediate circuit function see machine-specific circuit diagram “Main Input”.
The intermediate circuit drives the converter. It consists of a mains rectifier and one or
more storage capacitors.
The generated DC voltage is the basis for generating the three-phase current for driving
the motor. In order to attenuate the switch-on current peaks of the electronic capacitors,
the main voltage is connected first via resistors R55 and R56. After approximately 0.5
sec. the resistors are short-circuited via K4.

Main Input
For the main input function see the machine-specific circuit diagram “Main Input”.
The main voltage is supplied to the power board via plugs x3/1 and x3/2.
It connects transformer T1, lid release magnet via relay K1,2 and lid switch to voltage. If
the lid switches are closed, the following components are connected to the input.
The opto-coupler U2 (tells the micro controller that the lid is closed)
The relay K3 (can switch on the compressor)
The relays K2 (can switch on the fans)
The relays K4 (can switch on the intermediate circuit)

Relay Control
For the relay control function see the circuit diagram “Relay Driver”.
The relays are switched by the micro controller output ports via the relay driver D7. Relay
K4 is time-delayed by R39 and C25 with respect to PWM signed presence.

The thermistor temperature sensor measures the heat sink temperature.

The opto-coupler U2 LED is connected to the voltage after the lid is closed. U1 thus
signals to the micro controller that the lid is closed.
The exact sine valuation of the motor voltage generated in the converter allows an even
rotation of the motor at or above only approx. 30 RPM. Density gradient centrifugations
can thus be performed very well with these centrifuges.

Acceleration and braking follows selectable curves. They contain a time function together
with the max. slip speed for the motor. The slip is dominant; i.e.. if the preset acceleration
time is shorter than physically possible, acceleration does not follow the time but the
max. slip speed. This is always the case for curve 9. Here the desired times are so short
that they can never be achieved. Acceleration/braking in this case follows max. slip
speed and is thus achieved in the shortest possible time.

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Frequency Converter
The speed is measured and passed on to the processor both for determining the slip and
for regulating the speed. (See block diagram SM0020). A positive feedback regulator is
integrated into the software, compensating speed deviations to a very good +10 RPM

Frequency Converter
The converter consists of a power transistor module with corresponding drivers and the
current monitoring. Two transistors with their drivers are wired as a semi-bridge. Only
one transistor per semi-bridge is conductive at any one time. If uncomfortable voltage
peaks accidentally cause 2 transistors of one semi-bridge to become conductive at the
same time, they are usually destroyed. Often the CMOS circuits in the driver stages are
damaged as well, causing another failure in the future. As such damage is undetectable,
do not exchange transistors but the entire board.

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Main
Power

M
3

Interference
Suppression
Filter V=220V

NOTE: Consider in general that trying to repair within the converter (transistors and
drivers) should be avoided, because extensive experience and quite expensive
measuring equipment is required.

Brake Chopper
For the brake chopper function see the circuit diagram “Brake Chopper”.
The voltage regulator TL431 N3 generates the operating voltage for the brake chopper
via R37 and R38. The reference voltage is connected to the comparator N2C pin 8 and 9
and switches on transistor V38 and thus resistor R87 if the intermediate circuit voltage
exceeds approx. 360 V. If the intermediate voltage rises even further, at approx. 390V
the second comparator switches on the opto-coupler U1, which in turn signals over
voltage to the micro controller. Error message 25 or 35 appears and the drive slows
down freely.

Power Supply
For power supply function see the circuit diagram “Power Supply”.
Capacitor C1 and diode V39 form a voltage doubler with approx. 25 V at its output,
stabilized by N4 to 15 Volt. The unstabilized 25 VDC voltage supplies the switching
relays.

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5 Service Parts
5.1 Allegra 21

Electrical

P/N Description

365738 Board Assembly, Zent3 Power, 120V/ 5A

368950 Board Assembly, Zent3 Power, 230V/ 5A

B00455 Display/Control Assembly, Zent2, GS-15/ Allegra 21

365735 Touch Panel, Control, Zent2, GS-15 /Allegra 21
365745 Display/Control Board, Zent2, GS-15/ Allegra 21
366360 EPROM, GS-15/ Spinchron15 /Allegra 21

B00456 Display/Control Assembly, GS-15R/ Allegra 21R

365736 Touch Panel, Control, GS-15R Allegra 21R
365746 Display/Control Board, GS-15R/ Allegra 21R
366361 EPROM, GS-15R/Spinchron15R/ Allegra 21R

361349 Tach Generator Board

365754 Cable, Tach
365752 Rotor ID Plate Assembly
365787 Imbalance Sensor Assembly

365706 Latch 8140, 120V, UL (w/bar)

365757 Temperature Sensor (includes cable)
365758 Cable, Heatsink O/T Assy
365705 Cable, Ribbon, 50 Pin
365717 Fan, 120V, 60Hz, UL
927809 Fuse, T15A, 250V
361404 Plate Assembly, Contact, Imbalance
961845 Latch, 220V, Door Lock

361348 Motor Assembly Complete (DE 90-40)

961820 EMI Filter

961838 Fan, 220V
961836 Transformer
368973 Power Cord, 60 Hz, 15A

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Mechanical

P/N Description

361328 Motor Shaft Cone, 9/16 in. ID

368251 Magnet, Motor
364206 Boot, Rubber Seal, Motor Cover
961803 Vibration Damper, Motor Suspension
361329 Cover, Disc (plastic for motor)
361330 Cap for Motor Cover
361337 Motor Standoff
361335 Chamber Spacer
361374 Striker, Latch Lid
361341 Hinge Pin Assembly Set (hinge pin, washer & screw)
364958 Spring Kit, Door
363763 Pin, Hinge
363764 Spring, Left (62 mm)
363765 Spring, Right (62 mm)
360747 Front Panel, Metal
360753 Panel Assembly, Rear
360939 Panel Assembly, Rear
961804 Gasket, Rear Panel
360929 Barrier Ring, GS15
361331 Bowl, GS15
361355 Condenser
961816 Vibration Damper for Compressor
961813 Filter Dryer, Refer.
361361 String Assembly, Latch
961806 Gasket, Bowl, Chamber (9in. Dia.)
961837 Mounting Kit for Transformer
361339 Foot, Rubber, Suction
361377 Anti-Rotation Kit
369533 Drip Shield
367333 Overlay, Allegra 21R

Refrigeration

P/N Description

893332 Compressor, 50/60 Hz (POE oil) R134a

367815 Snubber, new style to use with relay 365804
361407 Tubing Kit, Copper, Refer.

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Tools and Supplies

P/N Description

883371 Tri-Flow Lubricant

964429 4 Weight Set (2,6,9,10g)
961660 Anti-Seize (961660)
267318 Metric Hex Key Set
267356 Drive Tester
306812 SPINKOTE Lubricant
335148 Silicone Vacuum Grease
339555 Solution 555
974231 Teflon Grease
361367 Rotor Tie-Down Bolt Assembly
361371 Spanner Wrench, T Handle, 10mm
347998 Arrow Label
339379 Rotor Cleaning Brush
339558 Rotor Cleaning Kit
964558 Wrench, Allen

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5.2 Allegra64R

Electrical
392066 Power Board, Zent 3, 230V/10A
A79151 Touch Panel Assembly (since 2011)
B02123 Control Display board Kit (since 2011)
365742 Display Board, Zent1 (obsolete use kit B03230)
365737 Panel, (touch) Control, Zent1(obsolete use kit B03230)
365782 Control Board, Zent1(obsolete use kit B03230)
366464 EPROM, Set, Av30, Zent1, (system & motor)
365752 Plate Assembly, Rotor I.D.
361349 Tach Generator Board
365754 Cable, Tach & Imbalance
365751 Board Assembly, Imbalance
970549 Switch, Power
893532 Bridge Rectifier, GBPC2506
365371 Latch Assembly
365793 Fan Assy, Condenser, 230V 50/60 Hz UL
361858 Fan, 220V, 50/60Hz, UL
393565 Motor Assembly
365197 Spacer, Top
365198 Spacer, Bottom
365757 Temperature Sensor, with cable
365881 Cable, Brake Resistor
365878 Cable, Flat Ribbon
365882 Cable Control to Display
365879 Harness, Front Panel
365769 Bracket, Mounting, Imbalance Board
365890 Bracket, Mounting, Power Board
893516 Dual Fuse Block
964744 Fuse 12A
927928 EMI Filter
974782 Resistor, Brake
974819 Bracket, Brake Resistor (2 req)
964637 Gray Scale Film, Imbalance
964643 Power Inlet
366455 Transformer
964654 Power Cord, 230V European
A21529 Power Cord, 208V USA
364173 Power Cord, UK
364185 Power Cord, Japan
974782 Brake Resistor
974819 Bracket, Brake Resistor

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Mechanical

964628 Vertical Damper, Motor, Thick Oil

964616 Horizontal Damper, Thin Oil (# 3)
367331 Overlay (Allegra 64R)
364206 Boot, Rubber Seal, Motor
964692 Cap, Motor Seal
361329 Disc Cover, Motor
964617 Rubber Mounts, Motor
964554 Screw, Hinge Pin M5 x 40
364201 Tie-Down Kit
358798 Foot
878544 Nut
816110 Screw
364137 Cover, Rear
365791 Panel, Front
890778 Nut, M3
364147 Sticker
964621 Pulley, Door
964622 Spring, Cable, Door
364219 Gasket, Door
961811 Washer, Sandpaper

Refrigeration

893302 Compressor, 240/220V 50 Hz for R404a

363532 Start-Up Kit 50 Hz
893303 Compressor, 200/208V 60 Hz for R404a
363533 Start-Up Kit 60 Hz
964606 Compressor, 220V (old style refrigerant)
964607 Relay, Compressor
961831 Mounting Kit, Compressor
964609 Capacitor, Starting
964610 Relief Cord, Compressor
964611 Cover, Compressor
364215 Condenser
364153 Chamber compl.
364933 Filter Dryer, Refer. (new style for R404)
964620 Valve, Schrader

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Tools and Supplies

883371 Tri-Flow Lubricant

267357 4 Weight Set (2,6,9,10g)
961660 Anti-Seize
267318 Metric Hex Key Set
267319 Metric Wrench Set
267356 Drive Tester
306812 SPINKOTE Lubricant
335148 Silicone Vacuum Grease
339555 Solution 555
974231 Teflon Grease
361367 Rotor Tie-Down Bolt Assembly
361371 Spanner Wrench, T Handle, 10mm
963893 Anti-Static Kit
961977 Arrow Label
974231 Grease, Marine (upper bearing)
339379 Rotor Cleaning Brush
339558 Rotor Cleaning Kit

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