Landau Labs SOP —CONFIDENTIAL

STANDARD OPERATING PROCEDURES BCC-LIC-023

Analysis of Cannabinoids by Agilent HPLC-PDA

Primary Contact
Levon Antonyan, PhD
Laboratory Director
Landau Laboratories, Inc.
[email protected]
213-275-1929

1.1 List of Analytes

Analyte CAS #
Cannabichromene (CBC) 20675-51-8
Cannabidiol (CBD) 13956-29-1
Cannabigerol (CBG) 25654-31-3
Cannabinol (CBN) 521-35-7
Cannabidiolic acid (CBDA) 1244-58-2
Cannabigerolic acid (CBGA) 25555-57-1
Cannabidivarin (CBDV) 24274-48-4
Cannabidivarinic acid (CBDVA) 31932-13-5
Δ9-Tetrahydrocannabinol (Δ9-THC) 1972-08-3
Δ8-Tetrahydrocannabinol (Δ8-THC) 5957-75-5
Δ9-Tetrahydrocannabinolic acid (THCA-A) 23978-85-0
Tetrahydrocannabivarin (THCV) 31262-37-0

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Chemical Structure of the Target Cannabinoids

Figure 1: Chemical structure of the twelve cannabinoids targeted for this analysis

1.2 Applicable Matrices

Cannabis matrices including but not limited to flower, trim, concentrates, kief, hash, bubble hash, and
resin. Chemically processed materials include extracts, oils, distillates, and isolates, as well as infused
oils, tonics, and edibles. Cosmetic matrices include topical, lotions, and salves. Hemp and its products
are also applicable under the scope of this SOP.

Matrix Notes
including but not limited to cured flower, trim, pre-rolls,
enhanced/infused pre-rolls, biomass, clone, fresh flower, fresh
Plant material frozen, fresh leaf, moon rock, popcorn buds, roots, seeds, sprouts,
stems/stalks, sugar leaf and any other plant matrices
including but not limited to extracts, oils, distillates, resins, hard
hash, ice water hash, oil hash, kief, bubble hash, isolates, caviars,
crumbles, crystalline solids, diamonds, full extract cannabis oils,
Concentrates and extracts live resins, live rosins, presses, Rick Simpson Oil (RSO), rosins,
shatters, sugar waxes, waxes and any other concentrate and/or
extract matrices
including but not limited to baked goods, beverages, capsules,
chocolates, candies, hard candies, lollipops, hard lozenges, ice
Ingestibles creams, liquid fats (oils), orally dissolving products, soft chews,
solid fats (butters, sat fats), suppositories, syrups, honeys,
tinctures and any other ingestible matrices
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 Landau Labs SOP —CONFIDENTIAL
including but not limited to gels, creams, ointments, massage oils,
Topicals bath salts, body oils, lotions, salves, soaps, shampoos,
transdermal patches and any other topical matrices.
including but not limited to hemp and its products and any other
Other matrices which contain cannabinoids and not indicated in this
table.

2.0 [Omitted per BCC]

3.0 Equipment and Standards
3.0 a) Instrumentation
Item Company
1290 Infinity II Multisampler or equivalent Agilent
1260 Infinity II Binary Pump or equivalent Agilent
1260 Infinity II Multicolumn Thermostat (MCT) or equivalent Agilent
1290 Infinity II Diode Array Detector Fixed Slit or equivalent Agilent

3.0 b) Other Equipment & Consumables

Other Equipment Company
Analytical Balance Mettler-Toledo or equivalent
SamplePrep Geno/Grinder SPEX or equivalent
Freezer Mill Cryogenic Grinder SPEX or equivalent
Centrifuge Beckman or equivalent
Vortex VWR or equivalent
Multi-Vortexer VWR or equivalent
Ultrasonic bath CO-Z or equivalent

Consumables Company Part #

InfinityLab Poroshell 120 EC-C18 3.0 x 50mm, 2.7µm or Agilent 699975-302T
equivalent
2-mL Amber Glass Sample vials with PTFE/Silicone Septa and PerkinElmer N9300696
Screw Caps; 100/Pkg or equivalent
1-mL Disposable Syringes with Luer-Lock tips or equivalent Any n/a
3-mL Disposable Syringes with Luer-Lock tips or equivalent Any n/a
10-mL Disposable Syringes with Luer-Lock tips or equivalent Any n/a
PTFE (Hydrophilic) Syringe Filters, 13 mm, 0.45 µm; 100/Pkg or Any n/a
equivalent
Nylon Syringe Filters, 45 mm, 0.45 µm; 100/Pkg or equivalent Any n/a
50-mL Polypropylene Self-Standing Centrifuge Tubes; 25/Pkg (Qty Any n/a
500 Tubes with Caps) or equivalent
Methanol HPLC grade or equivalent Any n/a
Acetonitrile HPLC grade or equivalent Any n/a
Formic acid Reagent grade or equivalent Any n/a
Water HPLC grade or equivalent Any n/a

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 Landau Labs SOP —CONFIDENTIAL
3.0 c) Procedures for Solutions, Standards & Reference Materials
Solution Preparation
Prepare Mobile phase A by adding 1mL of Reagent grade Formic acid in 1000 mL of HPLC grade water.
Fill it in HPLC mobile phase bottles, close it and mix well by inverting about 5-7 times. Perform a
degassing when necessary.
Prepare Mobile phase B by adding 0.5mL of Reagent grade Formic acid in 1000 mL of HPLC grade
Methanol. Fill it in HPLC mobile phase bottles, close it and mix well by inverting about 5-7 times. Perform
a degassing when necessary.

Standards Preparation
Prepare a 50-µg/mL working standard of the twelve standards by accurately pipetting 0.1 mL of
each cannabinoid standard into one 2-mL volumetric flask and dilute to the mark with methanol.
Perform scaling upon necessity (e.g. 1 mL of each analytical standard in total volume of 20 mL).
This will also serve as the Cal-L7 calibration standard.

Calibration Levels
Using adjustable micropipettes and 2-mL sample vials, prepare 20, 10, 5, 2, 1 and 0.5-µg/mL
calibration standards via dilutions of the 50-µg/mL working standard (Cal-L7). Use methanol as
diluent for all dilutions of these calibration standards, as follows:

• Cal-L7 (50 µg/mL): Pipette 1 mL of the working standard to a 2-mL sample vial

• Cal-L6 (20 µg/mL): 400 µL working standard + 600 µL methanol

• Cal-L5 (10 µg/mL): 200 µL working standard + 800 µL methanol

• Cal-L4 (5 µg/mL): 100 µL working standard + 900 µL methanol

• Cal-L3 (2 µg/mL): 40 µL working standard + 960 µL methanol

• Cal-L2 (1 µg/mL): 20 µL of working standard + 980 µL methanol

• Cal-L1 (0.5 µg/mL): 10 µL of working standard + 990 µL methanol

Cannabinoid Standards and Reference Numbers (12 Analytes)

Analyte Description Company Part #

1 mg/mL in Methanol; 1mL
Cannabichromene (CBC) Cerilliant/ Sigma-Aldrich C-143 or equivalent
ampule
1 mg/mL in Methanol; 1mL
Cannabidiol (CBD) Cerilliant/ Sigma-Aldrich C-045 or equivalent
ampule
1 mg/mL in Methanol; 1mL
Cannabigerol (CBG) Cerilliant/ Sigma-Aldrich C-141 or equivalent
ampule
1 mg/mL in Methanol; 1mL
Cannabinol (CBN) Cerilliant/ Sigma-Aldrich C-046 or equivalent
ampule
1 mg/mL in Acetonitrile; 1mL
Cannabidiolic acid (CBDA) Cerilliant/ Sigma-Aldrich C-144 or equivalent
ampule
1 mg/mL in Acetonitrile; 1mL
Cannabigerolic acid (CBGA) Cerilliant/ Sigma-Aldrich C-142 or equivalent
ampule
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1 mg/mL in Methanol; 1mL
Cannabidivarin (CBDV) Cerilliant/ Sigma-Aldrich C-140 or equivalent
ampule
Cannabidivarinic acid 1 mg/mL in Acetonitrile; 1mL
Cerilliant/ Sigma-Aldrich C-152 or equivalent
(CBDVA) ampule
Δ9-Tetrahydrocannabinol 1 mg/mL in Methanol; 1mL
Cerilliant/ Sigma-Aldrich T-005 or equivalent
(Δ9-THC) ampule
Δ8-Tetrahydrocannabinol 1 mg/mL in Methanol; 1mL
Cerilliant/ Sigma-Aldrich T-032 or equivalent
(Δ8-THC) ampule
Δ9-Tetrahydrocannabinolic 1 mg/mL in Acetonitrile; 1mL
Cerilliant/ Sigma-Aldrich T-093 or equivalent
acid (THCA-A) ampule
Tetrahydrocannabivarin 1 mg/mL in Acetonitrile; 1mL
Cerilliant/ Sigma-Aldrich T-094 or equivalent
(THCV) ampule
Sample Preparation
Sample preparation should be performed in accordance with the sample preparation
forms based on a sample matrix type, as follows:
MATRIX SAMPLE PREPARATION FORM
Flower, buds, plant and pre-roll products C-1
Extracts, oils, butters, waxes and other concentrated products C-2
Ingestibles, topicals, beverages and other infused products C-3

4.0 Liquid Chromatography Method Sensitivity

Calculated LOD and LOQ values
The limit of detection and limit of quantitation of each cannabinoid for each matrix is listed in the
tables below:
Table 1. LOD and LOQ values for each analyte in flower, buds, plant and pre-roll products
Compounds LOD (ug/mL) LOQ (ug/mL)
CBDVA 0.026 0.082
CBDV 0.052 0.164
CBDA 0.020 0.063
CBGA 0.029 0.091
CBG 0.084 0.266
CBD 0.090 0.286
THCV 0.062 0.197
CBN 0.012 0.037
d9 THC 0.072 0.229
d8 THC 0.088 0.281
CBC 0.035 0.113
THC-A 0.044 0.141
Table 2. LOD and LOQ values for each analyte in extracts, oils, butters, waxes and other
concentrated products
Compounds LOD (ug/mL) LOQ (ug/mL)
CBDVA 0.103 0.327
CBDV 0.206 0.656
CBDA 0.080 0.254

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 Landau Labs SOP —CONFIDENTIAL
CBGA 0.114 0.364
CBG 0.334 1.063
CBD 0.360 1.145
THCV 0.248 0.788
CBN 0.046 0.147
d9 THC 0.288 0.915
d8 THC 0.353 1.122
CBC 0.142 0.450
THC-A 0.178 0.565
Table 3. LOD and LOQ values for each analyte in ingestibles, topicals, beverages and other
infused products
Compounds LOD (ug/mL) LOQ (ug/mL)
CBDVA 0.003 0.008
CBDV 0.005 0.016
CBDA 0.002 0.006
CBGA 0.003 0.009
CBG 0.008 0.027
CBD 0.009 0.029
THCV 0.006 0.020
CBN 0.001 0.004
d9 THC 0.007 0.023
d8 THC 0.009 0.028
CBC 0.004 0.011
THC-A 0.004 0.014

5.0 Types, Frequency & Acceptance Criteria for Quality

Control (QC) samples
Initial calibration blank
An Initial Calibration Blank (ICB) is HPLC grade Methanol. Use the same diluting solvent
that is used to prepare external standards. If contamination is detected in the ICB it
originated from this source of Methanol. ICBs should be ran at the beginning of each
calibration run.
Initial Calibration Verification
An Initial Calibration Verification (ICV) is a solution of targeted method analytes of known
concentration obtained from a source different from the calibration standards. An ICV is
used to independently verify the external calibration. An ICV should be a second source
certified reference material. If standards from the same supplier as the calibration
standards are used, they should have different lot numbers.
Different lot numbers of Cerilliant standards are used to prepare the ICV from stock
solutions of 1000 ug/mL using methanol as diluent.

Stock Dilution Standard Volumetric

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 Landau Labs SOP —CONFIDENTIAL
(µg/mL) vol (µL) (µL)

1000 40x 50 2000

ICV Standard Volumetric
Dilution
(µg/mL) vol (µL) (µL)
25 - - -

Each calibration batch must include an Initial Calibration Verification (ICV). The percent
recovery must be between 70% to 130%.
Method blank
A Method Blank is an analyte free matrix to which all reagents are added in the same
volumes or proportions as used in the sample preparation and processed in exactly the
same manner as unknown samples. The Method Blank is HPLC grade MeOH. The
concentration of target analytes must not exceed their LOQ.
Laboratory control sample (LCS)
A Laboratory control sample is a sample prepared by adding a known quantity of the
target analytes to a sample matrix or to a matrix that is as closely representative of the
matrix being analyzed as possible. LCS sample is prepared using the 50 µg/mL working
standard (Cal-L7) as a spike solution. Use 500µL of this solution to spike approximately
0.5g weighed matrix sample (e.g. MCT oil) add 5mL of methanol as diluent. The amounts
and expected concentration are shown in the following table:

Stock conc (µg/mL) Spike amount (mL) Solvent volume (mL) Final conc (µg/mL)
50 0.5 5 5
Process the LCS sample after spiking in the same manner as usual unknown samples,
following all the procedures. The LCS sample must meet the criteria of Percent recovery
between 70% to 130%.
Laboratory Replicate Sample
A Laboratory Replicate Sample is a sub-sample taken of the representative sample used
for laboratory quality control purposes to demonstrate reproducibility. It is prepared and
analyzed in the identical manner as the representative sample. The results from replicate
analyses are used to evaluate analytical precision. A replicate sample is analyzed for
every 20 samples in an analytical batch. A replicate sample must have a relative percent
difference (RPD) of ≤30%.
Continuing calibration verification
Continuing Calibration Verification (CCV) samples are mid-range calibration standards
which check the continued validity of the initial calibration of the instrument. A CCV is
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 Landau Labs SOP —CONFIDENTIAL
prepared in the same manner as external calibration standards near the middle of the
calibration range. CCVs should be ran at the beginning of each analytical sequence and
every 10 samples thereafter and at the end of the analytical batch as a closing QC. The
CCV must meet the criteria of Percent recovery between 70% to 130%.
Continuing calibration blank
A Continuing Calibration Blank (CCB) is HPLC grade MeOH. Use the same diluting
solvent that is used to prepare external standards. If contamination is detected in the CCB
it originated from this source of MeOH. CCBs should be ran with every 10 samples and at
the end of an analytical batch as closing QC after the closing CCV.
Frequency of CCV samples in the analytical batch are presented in Table 1.
Table 1. Laboratory Quality Control samples, frequency, and acceptance criteria.
LQC Sample Frequency
ICV Once after each calibration
Method blank Once for each analytical batch. Prepared alongside unknowns
LCS Once for each analytical batch. Prepared alongside unknowns
Replicate Once for each analytical batch. Prepared alongside unknowns
At the beginning of each analytical run, each 10 samples thereafter and at the
CCV
end of an analytical batch as closing QC
At the beginning of each analytical run, each 10 samples thereafter and at the
CCB
end of an analytical batch as closing QC

6.0 Types, Frequency & Acceptance Criteria for

Calibration Standards
Calibration levels
Following 7 calibration levels for each analyte should be used for the initial calibration
1. Cal-L7 - 50 µg/mL
2. Cal-L6 - 20 µg/mL
3. Cal-L5 - 10 µg/mL
4. Cal-L4 - 5 µg/mL
5. Cal-L3 - 2 µg/mL
6. Cal-L2 - 1 µg/mL
7. Cal-L1 - 0.5 µg/mL
Co-efficient of determination (R2) ≥ 0.99 for curves for each analyte. Linear regression or
quadratic regression shall only be used for calibration curves. Curves shall not be
weighted at all or only weighted at ⅟x.

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 Landau Labs SOP —CONFIDENTIAL
Each calibration run must include following QC samples:
Initial calibration blank
An Initial Calibration Blank (ICB) is HPLC grade Methanol. Use the same diluting solvent
that is used to prepare external standards. If contamination is detected in the ICB it
originated from this source of Methanol. ICBs should be ran at the beginning of each
calibration run. Any analyte should not exceed LOQ.
Initial Calibration Verification
An Initial Calibration Verification (ICV) is a solution of targeted method analytes of known
concentration obtained from a source different from the calibration standards. An ICV is
used to independently verify the external calibration. An ICV should be a second source
certified reference material. If standards from the same supplier as the calibration
standards are used, they should have different lot numbers.
Different lot numbers of Cerilliant standards are used to prepare the ICV from stock
solutions of 1000 ug/mL using methanol as diluent.
Stock (µg/mL) Dilution Standard vol (µL) Volumetric (µL)
1000 40x 50 2000
ICV (µg/mL) Dilution Standard vol (µL) Volumetric (µL)
25 - - -
The percent recovery must be between 70% to 130%.
Re-run the initial calibration batch if Initial Calibration Verification (ICV), Laboratory control
sample (LCS) and Continuing Calibration Verification (CCV) is out of acceptance criteria
and if after reanalyzing the entire analytical batch for one time, the problem still persists.

7.0 Procedure for Analyzing Batch Samples

Laboratory Quality Control (LQC) Samples are run with each analytical batch. Samples
are placed in an analytical batch of no more than 20 samples. LQC samples are used to
adhere to good laboratory practice (GLP) in the performance of each analysis. Treat
method blanks, LCS, matrix spikes, and replicate samples in the same manner used to
prepare and analyze unknown samples.
Include the following LQC samples in each analytical batch.
• Method blank
• Laboratory control sample (LCS)
• Laboratory replicate sample (Replicate) or; Matrix spike
• Unknown samples
• Continuing Calibration Verification (CCV) at the beginning of each batch, after 10
samples, and at the end of the analytical batch to bracket the data.
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 Landau Labs SOP —CONFIDENTIAL
7.1 Setting up the LC Method
Configure your LC method parameters as follows using your chosen software package:

• Column: InfinityLab Poroshell 120 EC-C18 3.0 x 50mm, 2.7µm (Part # 699975-302T)

• Solvent A: 0.1% formic acid in DI water

• Solvent B: 0.05% formic acid in Methanol

• Autosampler Wash: Methanol

• Solvent Program:
Time (min.) Flow rate (mL/min.) %A %B
0 1.0 40 60
1.0 1.0 40 60
7.0 1.0 23 77
8.2 1.0 5 95

• Analysis Time: 9.5 min.; post time: 1.5 minutes

• Max Pressure: ~5800 psi

• Oven Temperature: 50ºC

• PDA Detector Channel(s):

Analytical Analytical Reference Reference
wavelength Bandwidth wavelength bandwidth
230 nm 4.0 nm 370 nm 60 nm
• Injection Volume: 1 or 5 µL

• Peakwidth: >0.0063 min (0.13 sec response time) (40 Hz)

8.0 Corrective Action Procedures

Corrective actions for out of control LQC samples are given in table 2.
Table 2. Laboratory Quality Control sample corrective actions for out of tolerance samples.
Laboratory Quality Control Acceptance
Corrective Action
Sample Criteria
Reanalyze entire analytical batch once. If method
blank is still greater than the LOQ for any analyte,
Method blank sample Not to exceed LOQ
locate the source of contamination then re-prep
samples and reanalyze.
Reanalyze the entire analytical batch, once. If
Laboratory control sample Percent recovery
problem persists, re-prep samples and reanalyze or
(LCS) 70% to 130%
re-run the initial calibration curve.
Reanalyze sample and associated replicate sample
Laboratory replicate sample RPD ≤30% once. If problem persists, re-prep samples and
reanalyze.

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 Landau Labs SOP —CONFIDENTIAL
Percent recovery Reanalyze sample and associated matrix spike
Matrix spike sample between 70% to sample once. If problem persists, re-prep samples
130% and reanalyze.
Reanalyze all samples that followed the last CCV
Percent recovery
Continuing Calibration that met the acceptance criteria. If CCV still fails, re-
between 70% to
Verification (CCV) run the initial calibration curve and all samples in
130%
the analytical sequence.

If any analyte is detected above any action level, as described in this chapter, the sample shall
be re-prepped and reanalyzed in replicate within another analytical batch.
For quantitative analyses, the re-prepped sample and its associated replicate must meet the
acceptance criteria of RPD ≤30%.
For qualitative analyses, the re-prepped sample and its associated replicate results must
concur.
If any LQC sample produces a result outside of the acceptance criteria, the laboratory cannot
report the result and the entire batch cannot be released to the client. The laboratory shall
determine the cause and take steps to remedy the problem until the result is within the specified
acceptance criteria.
If the laboratory determines that the result is a false-positive or a false-negative, the Bureau of
Cannabis Control may ask for the laboratory to re-sample or re-test.
The laboratory shall compile and generate one LQC sample report for each analytical batch that
includes LQC acceptance criteria, measurements, analysis date, and matrix.
If the result of an analyses is outside the specified acceptance criteria in the above table, the
laboratory shall determine the cause and take steps to remedy the problem until the result is
within the specified acceptance criteria.

9.0 Test Method Calculations

Final reported concentration calculations
The calculations for the final reported concentration are performed based on the dilution factor.
Dilution factor is calculated in the Dilution Factor Calculator excel spreadsheet by copying the
sample ID from the LIMS system and entering weighed amounts and dilutions from the
benchsheet, the formula of the excel spreadsheet automatically calculates the dilution factor.
The formula for the dilution factor (DF) calculation is the following:
𝐸𝑉 𝐷𝑉1 𝐷𝑉2 𝐷𝑉3 10
𝐷𝐹 = × × × ×
𝑊 𝑆𝑉1 𝑆𝑉2 𝑆𝑉3 𝐼𝑉
where: EV – extractant volume in mL
W – weighed sample amount in g
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st nd rd
DV1, DV2, DV3, - 1 , 2 and 3 dilution diluent volumes respectively in mL
SV1, SV2, SV3, - 1st, 2nd and 3rd dilution sample volumes respectively in mL
IV – injection volume (If the injection volume is 2 µL the dilution factor will be multiplied by 5.) in µL.

The dilution factor is further entered to the instrument software under Dilution Factor field. The
software automatically calculates the final concentrations considering the dilution factors and
prints them on the report. Values are further entered in the LIMS system where the final report
is generated, automatically considering the units per serving, servings per container and unit
weights as well as moisture values (reporting per dry weight) wherever it’s applicable, based on
cannabis product matrix type and required COA format.
Cannabinoid calculations
𝑚𝑔 𝑉𝑜𝑙 (𝐿)
%= 𝑥 𝑥 100
𝐿 𝑀𝑎𝑠𝑠 (𝑚𝑔)
𝑚𝑔 𝑚𝑔 𝑉𝑜𝑙 (𝐿)
= 𝑥
𝑔 𝐿 𝑀𝑎𝑠𝑠 (𝑔)
𝑚𝑔 𝑚𝑔 𝑉𝑜𝑙 (𝐿) 𝑔
= 𝑥 𝑥
𝑢𝑛𝑖𝑡 𝐿 𝑀𝑎𝑠𝑠 (𝑔) 𝑈𝑛𝑖𝑡
𝑀𝑎𝑠𝑠 (𝑔)
𝐷𝑒𝑛𝑠𝑖𝑡𝑦 =
𝑉𝑜𝑙𝑢𝑚𝑒 (𝑚𝐿)
𝑈𝑛𝑖𝑡 𝑚𝑎𝑠𝑠 = 𝑈𝑛𝑖𝑡 𝑣𝑜𝑙 (𝑚𝐿) 𝑥 𝐷𝑒𝑛𝑠𝑖𝑡𝑦 (𝑔/𝑚𝐿)
QC calculations
[𝑆𝑎𝑚𝑝𝑙𝑒 𝑐𝑜𝑛𝑐 − 𝐷𝑢𝑝𝑙𝑖𝑐𝑎𝑡𝑒 𝑐𝑜𝑛𝑐]
𝑅𝑃𝐷 = 𝑥 100
[𝑆𝑎𝑚𝑝𝑙𝑒 𝑐𝑜𝑛𝑐 + 𝐷𝑢𝑝𝑙𝑖𝑐𝑎𝑡𝑒 𝑐𝑜𝑛𝑐 ]/2
𝑆𝑎𝑚𝑝𝑙𝑒 𝑟𝑒𝑠𝑢𝑙𝑡
𝑃𝑒𝑟𝑐𝑒𝑛𝑡 𝑟𝑒𝑐𝑜𝑣𝑒𝑟𝑦 = 𝑥 100
𝐸𝑥𝑝𝑒𝑐𝑡𝑒𝑑 𝑟𝑒𝑠𝑢𝑙𝑡

Unit conversions
𝑚𝑔
⁄𝐿 = 𝑝𝑝𝑚
𝑚𝑔
⁄𝑘𝑔 = 𝑝𝑝𝑚
𝑚𝑔
⁄𝑚𝐿 = 𝑝𝑝𝑡
𝑚𝑔
⁄𝑔 = 𝑝𝑝𝑡

% = 𝑝𝑝ℎ
Decarboxylated totals
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 Landau Labs SOP —CONFIDENTIAL

𝑇𝑜𝑡𝑎𝑙 𝑇𝐻𝐶 = 𝑇𝐻𝐶𝐴 𝑥 0.877 + 𝑇𝐻𝐶

𝑇𝑜𝑡𝑎𝑙 𝐶𝐵𝐷 = 𝐶𝐵𝐷𝐴 𝑥 0.877 + 𝐶𝐵𝐷
Decarboxylation
Decarboxylation is the loss of the carboxylic acid group from heating or chemical processes
resulting in the evolution of carbon dioxide gas. In the process the acidic forms of cannabinoids
are converted to their neutral “activated” forms. The decarboxylation constant for the conversion
of THCA into THC is 0.877 and is obtained by taking the ratio of the molecular weights of THC
and THCA:

10.0 Potential Interferences with the Analysis

10.0 a) Power Outage
In the event of a power outage, the analytical batch run will be restarted followed with the
verification of the LQC samples

10.0 b) Insufficient Mobile Phases

In the event of Insufficient Mobile Phases, fresh mobile phase will be prepared and the
system will be purged. If these steps do not solve the issue, a maintenance visit from the
instrument vendor will be scheduled.

10.0 c) Presence of an Air Bubble

In the event of an air bubble, the system will be purged. If this step does not solve the
issue, a maintenance visit from the instrument vendor will be scheduled.

10.0 d) Presence of a Leak

In the event of a leak, the source of the leak will be identified. All connectors will be
tightened, and the column will be replaced if required. If these steps do not solve the
issue, a maintenance visit from the instrument vendor will be scheduled.

11.0 ISO/IEC 17025

In process with A2LA.

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12.0 Document Revision History

Revision Description of Changes Initials Date

x Initial version LA Dec 2019

3.0 Updated content LA 06-04-2020

Approved By:

Name Function Signature Date

Georg Kallert Executive 06-05-2020

Levon Antonyan Lab Director 06-05-2020

References
1. Cannabis oil vs hemp seed oil; Cannabis oil, CDB Oil, Medical Marijuana. http://cbd.org/cannabis
-oil-vs-hemp-oil
2. Current BCC Regulations. https://bcc.ca.gov/law_regs/cannabis_order_of_adoption.pdf
3. Test Methods – Standard Operating Procedures Form- https://bcc.ca.gov/clear/bcc_lic_023.pdf

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