The Essential Systems & Consumables for

HIGH PERFORMANCE
LC PURIFICATION
& MASS SPECTROMETRY
 Aim high. Discover more. The Advion® x Interchim® unity.
Dear Customers,

Sharing a customer-focused company vision, a taste for excellence with state-of-the-art product solutions and working hand in hand
for eight years, it was natural that Advion®, a well known player in the world of mass spectrometry and chromatography products, and
Interchim®, a global provider of instrumentation and consumables for life sciences, research and industry would strengthen their ties to
accompany their customers and partners even further.
It is with great joy to announce our commercial combination- a joy that becomes tangible with the first release of our shared catalog
dedicated to the essential instruments and consumables for High Performance LC purification.
From sample injection, purification, detection, analysis and more, you have in your hands everything you need to increase the productivity
of your laboratory and to achieve expert results daily.
You can also find all our products on our websites www.interchim.com, www.flash-chromatography.com and www.advion.com as
well as our application notes and tips and tricks in our blog articles. Both technical and support services from Interchim® and Advion®
remain at your disposal to help you overcome your challenges and to choose the most appropriate product solutions.
From our respective headquarters in France and the United States, our subsidiaries, and our independent distributors network, we thank
you for your confidence and we are proud to accompany you in the success of your projects.
Interchim x Advion

“I am really excited about the perspective of “I am thrilled to increase collaboration

this commercial combination with Advion®. with Interchim®. Our expanded solution
As a guarantee of a promising future, it deck and global scale will provide a
represents today for Interchim®, formidable platform for growth – offering
its customers, suppliers and partners a novel media, columns, prep LC, HPLC,
perfect fit. flash chromatography, mass spectrometry,
The strengths of the two entities, already sample prep, and much more.
working together successfully, will be Both Advion and Interchim customers
enhanced by this new step and will enable us to reach and develop will benefit from an immediately expanded workflow solution
unparalleled scientific solutions and technological platforms for our portfolio. Our mutual dedication to customer-focused solutions
customers aroundthe world.” will continue, as will our dedication to world-class service.
Lionel Boch, Président du Directoire, Interchim Together with Interchim, we have built strong relationships which
persist to this day and will continue to flourish with this incredible
opportunity for commercial combination.”
David B. Patteson, CEO, Advion

Interchim and Advion led the market by being

the first to develop integrated Flash-MS and
seamlessly coupling Advion’s high performance
expression® Compact Mass Spectrometer with
Interchim’s puriFlash® purification systems.

2 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 Advion & Interchim® 2020 Conference Schedule
Join our teams across the globe at an upcoming conference, symposium or trade show. Connect with us in nearby locations to see the
instruments live and experience both Interchim and Advion systems. Please note: this schedule is subject to change.

January June
. 27-29 - Orleans, France - 4th Chemical Ligation Meeting . 11-12 - Marseille, France - SFST - Symposium Francophone de
. 28-30 - Dallas, TX - Texas Hemp Convention Synthèse Totale
. 12 - New York, NY - MARM
February . 15-18 - San Diego, CA - 17th International Congress on Catalysis
. 05-07 - Madrid, Spain - 17th Iberian Peptides Meeting . 21-24 - Krakow, PL - Polish-Czech Mass Spectrometry Meeting,
. 13-15 - Las Vegas - USA CBD Expo PCMSM
. 19-20 - Boston, MA - Northeast Cannabis Business Conference . 28 - Bellingham, WA - NORM
March June - July
. 01-04 - Münster, Germany - DGMS . 28-01 - New York, NY- NMCS - National Medicinal Chemistry
. 01-05 - Chicago, IL - Pittcon Symposium, ACS
. 08-11 - Seattle, WA - US HUPO
. 11-13 - Münster, Germany - BioChemistry 2020 July
. 15-19 - Aussois, France - RECOB - Rencontres en Chimie . 05-09 - Prague, CZ - EPHAR 8th European Congress of
Organique Biologique Pharmacology Tentative
. 17-20 - Mondonville, France - JIREC - Journées d’Innovation et . 05-10 - Namur, Belgium - BOSS XVII 17th Belgian Organic
de Recherche pour l’Enseignement de la Chimie Synthesys Symposium
. 20-22 - Boston, MA - NECANN . 07-10 - Nancy, France - MBP - International Conference on Metal-
. 22-25 - Freiburg - Frontiers in Medicinal Chemistry Binding Peptides: Methodologies and Applications
. 22-26 - Philadelphia, PA - ACS Spring . 18-23 - Corvallis, OR - BCCE
. 26-28 - Denver, CO - NOCO . 25-30 - San Francisco, CA - ICNPR 10th International Congress of
Natural Products Research
March - April . 26-29 - Fort Lauderdale, FL - NACRW
. 29-02 - Palm Springs, CA - MSACL
. 30-02 - Rennes, France - IGCS/Green Cat - 4th International August
Green Catalysis Symposium . 06-08 - Uncasville CT - USA CBD Expo
. 30-02 - Oxford, MS - ICSB Oxford . 14-15 - Nashville - Southern Hemp Expo
. 31-03 - Müchen - Analytica . 16-18 - San Francisco - ACS
. 23-28 - Cap d’Agde, France - GECO - Groupe d’Etudes en Chimie
April Organique
. 04-06 - Pueblo, CO - ICR - Institute of Cannabis Research
. 06-08 - Baltimore, MD - Cannabis Science East August - September
. 13-16 - Atlanta, GA - SS AOAC (Southern Section) . 29-04 - Rio De Janerio - IMSC - International Mass Spectrometry
. 17-19 - University of Kansas - MIKI Medicinal Chemistry Meeting- Conference
in-Miniature . 30-03 - Lisbon, Portugal - EucheMS
. 21-23 - Las Vegas - Cannabis conference . 31-02 - Portland, OR - Cannabis Science West
. 27-29 - Montpellier, France - GFPP - Cell-Penetrating Peptides:
Next generation delivery systems September
. 06-10 - Base - EFMC-ISMC 2020
May . 07-09 - Münster - Orchem
. 06-07 - Fort Detrick, Frederic, MD - Biomedical Research . 21-24 - Boulder, CO - Boulder Peptide Symposium
Equipment and Supplies Exhibit at Fort Detirck . 21-22 - Pasadena - Cannmed
. 07-09 - Atlanta, GA - USA CBD Expo
. 12-13 - Bogota, Colombia - Cannacienca September - October
. 12-14 - Boston, MA - TIDES . 29-02 - Regensburg - Joint 8th European Lipidomics Meeting - 6th
. 28-30 - New York - CWBC Lipidomics Forum - Tentative
. 18-19 - Midlothian, VA - VADCC - VirginiaDrugDiscoveryRx: October
Targets to Translation . 07-08 - Lausanne, CH - ILMAC
. 20-21 - Bethesda, MD - NIH Spring Biomedical Research Exhibit . 18-21 - Rochester, NY - NERM
. 24-28 - Winnepeg, Canada - Canadian Chemistry Conference . 21-23 - Springfield, MO - MWRM Midwest Regional Meeting ACS
. 24-28 - Camaret Sur Mer, France - GECOM-CONCOORD - . 22-23 - Beatenberg, CH - SGMS Meeting
chimie organométallique et à la chimie de coordination . 22-23 - Leipzig - DPhG
. 27-29 - Columbus, OH - CERM . 29-30 - Las Vega, NV - Supply Side West
. 28-29 - Marseille, France - RCOM - Rencontre de Chimie Organique
de Marseille November
. 08-11 - US Hemp Growers Conf. Seed to sale - Tentative Event
May - June . 16-18 - Princeton, NJ - Eastern Analytical Symposium
. 31-04 - Houston, TX - ASMS
December
. 11-13 - USA CBD Expo - Tentative Event
. 15-20 - Honolulu, HI - Pacifichem 2020 (every 5 years)
. 16-18 - Las Vega, NV - MJBizCon

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 3

4 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com
Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 5
 Genius makes Easy & Secured Purifications
1. TLC method development 2. Genius proposal
TLC 1 TLC 2
Among the columns proposed by Genius, we have
selected a PF-15SIHP-F0012, which was available
1 in stock:

2 TLC to Flash & Prep (Normal Phase) UPLOAD RESET

3
1
Crude sample 100 mg

Solvent 1 Toluene 90 %
2 Additive
3
4
5 Solvent 2 Methanol 10 % Additive

1 Select your SELECT A COLUMN

Mobile phase: Mobile phase: compound(s)
87% DCM / MeOH 13% 90% Toluene / MeOH 10% 0.9 of interest
Column Stock
PF-30SIHP-F0012
0.8 Rf 0.57
Compound Rf CV Compound Rf CV PF-15SIHP-F0012
PF-30SIHP-F0025
1 0.95 1.05 1 0.57 1.75 0.7
Rf 0.31 PF-30SIHP-F0040
PF-30SIHP-F0080
2 0.68 1.47
2 0.31 3.23 PF-30SIHP-F0120
Rf 0.25
3 0.64 1.56 0.6
Top 3 columns
3 0.25 4
∆CV3-2 = 0.09 4 0.18 5.56 0.5
Rf 0.18
INJECTION MODE
5 0.14 7.14
Rf 0.14 The crude is fully soluble
0.4
in the below conditions:
Rf of compounds 2 and 3 are close, the separation will not be
Toluene 98.9%
optimal on Flash column.
0.3

Methanol 1.1%
Genius provides new TLC conditions (TLC 2) in order to 0.2
increase ∆Rf & ∆CV. Yes No

0.1 Liquid mL (max 1.66 mL)

R N
0 Dry load PF-DLE-F0004

R N R GO TO MANUAL METHOD GO TO RUN

6 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

3. Flash conditions

Device: puriFlash® 5.020

Solvents: A: Toluene
B: Methanol
Column: PF-15SIHP-F0012
Flow rate: 15mL/min
Injection mode: Solid deposit with celite
(Dry-load F0004)
Crude sample: 100mg
Detection: UV 265nm
Pressure: 4bar
Elution conditions:
t (min) A (%) B (%)
00:00 98.9 1.1
03:27 98.6 1.4
08:17 97.3 2.7
15:52 88.4 11.6
20:00 77 23
26:13 77 23

Small, but Limitless

250mL/min@up to 250bar

Purge

Method
GENIUS MODE Compound 1 Compound 2 Compound 3 Compound 4 Compound 5
+
Sample
Queue

Live run

Reprocess

Admin

Settings

Log in

Log out

Off

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 7

Ultra Performance Flash Purification

Small, but Mighty Cross-over Flash /Prep

300mL/min@up to 20bar 250mL/min@up to 50bar
The Partner of Daily Challenges
300mL/min@up to 20bar

Process-Kilo-Lab
400mL/min@up to 35bar

Lab-Productivity
300mL/min@up to 20bar Process - Kilo-Lab
825mL/min@up to 15bar

Process Purification
 Ultra High Performance Purification

The Ultra-Purification
Small, but Limitless 125mL/min@up to 400bar
Complex Purifications with Confidence 250mL/min@up to 250bar
250mL/min@up to 125bar

Flash Load Boost

& Go & Go & Go
Accuracy

Purification of Complex
The Peptides Specialist Mixtures of Peptides
125mL/min@up to 250bar UP3
250mL/min@up to 125bar Ultra Performance
Proteins Purification
100mL/min@up to 50bar
Peptides & Proteins Purification
 Ultra Performance Flash Purification Small Organic molecules
Impurities Identification
Natural Products
Peptides, Oligonucleotides
Proteins, Bio-drugs
Metabolites Isolation
Traces Enrichment

Accuracy
Small but mighty
Concentrated technology for unmatched performance.
300
& Repeatability

mL/min Designed for routine flash purifications, the technology and unique quality of the pump will take you much further.
up to
Increase the pressure, the puriFlash® XS 520Plus will nevertheless offer precision, linearity and repeatability
800G and allows you to perform complex and sophisticated purifications.
20 No matter whether you're an expert or not, Genius will support you to achieve the best purification possible.
bar
InterSoft® X
“Genius” Artificial Intelligence embeded
User-friendly
Click & Drag Gradient
Unit Control Intelligent Isocratic Mode Solvent tray w/drainage system
15" touch screen
Remote Control Injection
USB x4 Liquid or Dry-Load injection
w/column equilibration.

Load
& Go an automated
4 port electric PPS valve
manages solid injection
(Dry-Load)
Fraction Collector
2 long racks
112 tubes 18x150mm
Drainage system
Fume enclosure

Quaternary Gradient Pump

Air purge

PF-XS 520Plus + pack_Ultra

Modular & Easy to Maintain
Built to last

Column i.d. up to
=> 90mm

Detection
PF-XS 520Plus => UV: 200-400nm
(Multi wavelength & Scan collection
Smallest footprint Spectral view & Purity confirmation)
on the market Flow Cell: 0.3mm / 40µL

Integrated flash columns holder

=> UV: 200-800nm

Flash Boost
& Go & Go P/N: PFXS50

10 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 Small Organic molecules
Impurities Identification
Natural Products
Peptides, Oligonucleotides
Proteins, Bio-drugs
Metabolites Isolation
Traces Enrichment

Accuracy
The partner of daily challenges
Access automation and more security. Thanks to embedded technology (RFID, leak & level sensors, ...)
300
& Repeatability
the working time is now devoted entirely to purification and no longer to the management of the instrument.
up to mL/min

800G InterSoft® X

20 “Genius” Artificial Intelligence embeded

User-friendly
bar Click & Drag Gradient
Intelligent Isocratic Mode

Unit Control
15" touch screen Solvent tray w/drainage system
Remote Control
USB x8 - RJ45

Pre-columns holder

Quaternary Gradient Pump

Air purge

Fraction Collector Injection

3 racks Gen5 Liquid or Dry-Load injection
132 tubes 18x150mm w/column equilibration.
Drainage system
Fume enclosure Load & Gotm technology:
an automated
4 port electric PPS valve
manages solid injection

Column i.d. up to
=> 90mm

Modular & Easy to Maintain

Built to last

Detection
UV: 200-400nm
Safety: Pack-UVextended: UV: 200-800nm
Leak detection (Multi wavelength & Scan collection
Solvent & waste level monitoring Spectral view & Purity confirmation)
Integrated flash columns
& pre-columns holder Flow Cell: 0.3mm / 40µL
Pack-iELSD

Flash Load Boost

& Go & Go & Go P/N: PFG5A0

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 11

 Process Purification Small Organic molecules
Impurities Identification
Natural Products
Peptides, Oligonucleotides
Proteins, Bio-drugs
Metabolites Isolation
Traces Enrichment

Accuracy
Process - Kilo-Lab
Reliability and robustness with significant loading capacity
400
& Repeatability

mL/min Its reliability and robustness over time, as well as security features are the essential assets to make semi-
up to
continuous productions effective with up to hundreds of grams of the compound of interest.
1600G
Continuity of production on the same device independently of the scale-up factor.
35
bar
InterSoft® X
“Genius” Artificial Intelligence embeded
User-friendly
Click & Drag Gradient
Unit Control Intelligent Isocratic Mode Solvent tray w/drainage system
15" touch screen
Remote Control Injection
USB x4 Liquid or Dry-Load injection
w/column equilibration.

Fraction Collector
2 long racks
112 tubes 18x150mm
Drainage system
Fume enclosure

Quaternary Gradient Pump

Air purge

Modular & Easy to Maintain

Built to last

Column i.d. up to
=> 100mm

Detection
UV: 200-400nm
(Multi wavelength & Scan collection
Smallest footprint Spectral view & Purity confirmation)
on the market Flow Cell: 0.3mm / 40µL
Pack-UVextended: UV: 200-800nm

Pack-ColHolder:
Integrated flash columns holder

Flash Boost
& Go & Go P/N: PF535XL

12 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 Small Organic molecules
Impurities Identification
Natural Products
Peptides, Oligonucleotides
Proteins, Bio-drugs
Metabolites Isolation
Traces Enrichment

Accuracy
Process - Kilo-Lab
Reliability & robustness with significant loading capacity.
825
& Repeatability

mL/min Its reliability, its robustness over the time as well as its security features are the essential assets to make
up to
150mm i.d. semi-continuous productions effective up to Kgs of compound of interest.
Col. Continuity of production on the same device independently of the scale-up factor.
15
bar
InterSoft® X
“Genius” Artificial Intelligence embeded
User-friendly
Click & Drag Gradient
Intelligent Isocratic Mode

Unit Control
15" touch screen Solvent tray w/drainage system
Remote Control
USB x8 - RJ45

Quaternary Gradient Pump

Washing Disc

Fraction Collector
3 racks Gen5
44 tubes 29.5x200mm
Drainage system
Fume enclosure

Column i.d. up to
=> 150mm

Modular & Easy to Maintain

Built to last

Detection
UV: 200-400nm
Safety: Pack-UVextended: UV: 200-800nm
Leak detection (Multi wavelength & Scan collection
Solvent & waste level monitoring Spectral view & Purity confirmation)
Pack-Process:
Integrated flash columns holder Flow Cell: 0.3mm / 80µL
& pre-columns holder
Flash Load Boost
& Go & Go & Go P/N: PFG5L0

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 13

 Ultra Performance Flash Purification
Flash Purification Flash Purification
PF-XS 520Plus* PF-5.020
Pump
Flow rate 300mL/min 300mL/min
Pressure max. 20bar 20bar
Quaternary Gradient yes yes
Air purge yes yes
Washing discs no no
Pump washing discs (pack) no no

Detector *Plus _UVextended _Ultra

UV: 200 - 400nm multi wavelength & scan collection yes no no yes
UV: 200 - 800nm multi wavelength & scan collection no yes yes pack-UVextended
Spectral view & purity confirmation yes yes
iELSD Detection (pack) no pack-iELSD
pH/Conductimeter no no

Injection *Plus _UVextended _Ultra

4 port electrical valve no no yes yes
6 port electrical valve w/loop no no
6 port + 10 port electrical valves w/loop no no
Injection mode: liquid - Dry-Load yes yes

Column Selection valve

14 port /6 position electrical valve no no
6 port electrical valve no no

System Optimization
Tubings - after pump 1/8'' x 1.6mm id 1/8'' x 1.6mm id
Flow cell-optical length 0.3mm/40µL 0.3mm/40µL

Columns Holder
Integrated yes yes
Pre-column holder no yes

Fraction Collector
2 long racks 3 racks Gen5
Regular Collector
112 tubes 18x150mm 132 tubes 18x150mm

Unit control
Touch screen 15" yes yes
USB 4 8
RJ45 yes yes

Software
Intersoft® X yes yes

Safety
Leak detection (pump, FC, iELSD, holders) no yes
Solvent tray w/drainage system yes yes
Collector w/drainage sytem yes yes
Solvent level monitoring no yes
RFID no yes
Fume Encloser yes yes

Size
W: 14" - 35.5cm W: 15.75" - 40cm
D: 18.5" - 47cm D: 20.0" - 51cm
H: 30" - 77cm H: 29.5" - 75cm

14 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 Process Purification
Flash Purification Flash Purification Process Flash Purification Process Flash Purification Process
PF-5.050 PF-5.020-5X PF-535-XL PF-5.015-XL

250ml/min 300mL/min 400mL/min 825mL/min

50bar 20bar 35bar 15bar
yes yes yes yes
yes yes yes no
yes no no yes
no no no no

yes yes yes yes

pack-UVextended pack-UVextended pack-UVextended pack-UVextended
yes yes yes yes
pack-iELSD pack-iELSD no no
no no no no

no no no no
yes yes no no
no no no no
yes yes yes yes

no yes no no
no no no no

1/8'' x 1.6mm id 1/8'' x 1.6mm id 1/8'' x 1.6mm id 1/8'' x 2.4mm id

0.3mm/40µL 0.3 mm/40µL 0.3mm/40µL 0.3mm/80µL

yes yes pack-ColHolder pack-Process

yes no no pack-Process

3 racks Gen5 3 racks Gen5 2 long racks 3 racks Gen5

132 tubes 18x150mm 132 tubes 18x150mm 112 tubes 18x150mm 42 tubes 29.5x200mm

yes yes yes yes

8 8 4 8
yes yes yes yes

yes yes yes yes

yes yes no yes (except holders)

yes yes yes yes
yes yes yes yes
yes yes no yes
yes yes no yes
yes yes yes yes

W: 15.75" - 40cm W: 15.75" - 40cm W: 14" - 35.5cm W: 15.75" - 40cm

D: 20.0" - 51cm D: 20.0" - 51cm D: 18.5" - 47cm D: 20.0" - 51cm
H: 29.5" - 75cm H: 29.5" - 75cm H: 30" - 77cm H: 29.5" - 75cm

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 15

 Ultra High Performance Purification Small Organic molecules
Impurities Identification
Natural Products
Peptides, Oligonucleotides
Proteins, Bio-drugs
Metabolites Isolation
Traces Enrichment
Accuracy
Small but limitless
Maximum Versatility and Flexibility.
250
& Repeatability

mL/min
The system brings a unique performance in all circumstances. It adapts to all needs from routine purification
up to to complex mixtures, impurity separation, or trace enrichment.
80mm i.d.
Col. It is continuously ready to start multiple purifications in normal or reverse phase, flash or prep.
250
bar
InterSoft® X
“Genius” Artificial Intelligence embeded
User-friendly
Click & Drag Gradient
Intelligent Isocratic Mode

Unit Control
15" touch screen Solvent tray w/drainage system
Remote Control
USB x8 - RJ45

Pre-columns holder

Quaternary Gradient Pump

Air purge
Washing Disc

Fraction Collector Injection

3 racks Gen5 Liquid or Dry-Load injection
132 tubes 18x150mm w/column equilibration.
Drainage system
Fume encloser Load & Gotm technology:
an automated
6 port + 10 port electric valves
manages liquid & solid injection

Column i.d. up to
=> 80mm

Modular & Easy to Maintain

Built to last

Detection
UV: 200-400nm
Safety: Pack-UVextended: UV: 200-800nm
Leak detection (Multi wavelength & Scan collection
Solvent & waste level monitoring Spectral view & Purity confirmation)
Integrated columns holder
Pack_Multi: column selection valve Flow Cell: 0.3mm / 40µL
Pack-iELSD
Flash Load Boost
& Go & Go & Go P/N: PFG5E0

16 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 Small Organic molecules
Impurities Identification
Natural Products
Peptides, Oligonucleotides
Proteins, Bio-drugs
Metabolites Isolation
Traces Enrichment
Accuracy
The Ultra-Purification
Method development and purification of rare and high added value compounds.
125
& Repeatability
From method development to purification on the same instrument, the system offers more flexibility, saving
up to mL/min
time. It is compatible with a sub-2 micron column for maximum separating power.
60mm i.d.
Col.
400
bar

InterSoft® X
“Genius” Artificial Intelligence embeded
User-friendly
Click & Drag Gradient
Intelligent Isocratic Mode

Unit Control
15" touch screen Solvent tray w/drainage system
Remote Control
USB x8 - RJ45

Pre-cols holder

Quaternary Gradient Pump

Washing Disc

Injection
Fraction Collector Liquid or Dry-Load injection
3 racks Gen5 w/column equilibration.
132 tubes 18x150mm
Drainage system Load & Gotm technology:
an automated
6 port + 10 port electric valves
manages solid injection

Column i.d. up to
=> 60mm

Modular & Easy to Maintain

Built to last

Detection
PF-5.400: UV: 200-400nm
PF-5.400 + Pack-UVextended: UV: 200-800nm
(Multi wavelength & Scan collection
Spectral view & Purity confirmation)
Integrated cols holder
Flow Cell: 1.0mm / 20µL
Flash Load Boost
& Go & Go & Go P/N: PFG5F0

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 17

 Preparative Preparative Ultra-Prep
PF-5.125 PF-5.250 PF-5.400
Pump
Flow rate 250mL/min 250mL/min 125mL/min
Pressure max. 125bar 250bar 400bar
Quaternary Gradient yes yes yes
Air purge yes yes no
Washing discs yes yes yes
Pump washing discs (pack) pack-PWD pack-PWD pack-PWD

Detector
UV: 200 - 400nm multi wavelength & scan collection yes yes yes
UV: 200 - 800nm multi wavelength & scan collection pack-UVextended pack-UVextended pack-UVextended
Spectral view & purity confirmation yes yes yes
iELSD Detection (pack) pack-iELSD pack-iELSD
pH/Conductimeter no no no

Injection
4 port electrical valve no no no
6 port electrical valve w/loop yes no no
6 port + 10 port electrical valves w/loop no yes yes (1/16'' & 0.75mm)
Injection mode: liquid - Dry-Load yes yes no

Column Selection valve

14 port /6 position electrical valve no no no
6 port electrical valve pack-Multi pack-Multi no

System Optimization
Tubings - after pump 1/8'' x 1.6mm id 1/8'' x 1.6mm id 55 - 1/16'' x 0.50mm id
Flow cell-optical length 0.3mm/40µL 0.3mm/40µL 1.0mm/20µL

Columns Holder
Integrated yes yes yes
Pre-column holder yes yes no

Fraction Collector
3 racks Gen5 3 racks Gen5 3 racks Gen5
Regular Collector
132 tubes 18x150mm 132 tubes 18x150mm 132 tubes 18x150mm

Unit control
Touch screen 15" yes yes yes
USB 8 8 8
RJ45 yes yes yes

Software
Intersoft® X yes yes yes

Safety
Leak detection (pump, FC, iELSD, holders) yes yes yes
Solvent tray w/drainage system yes yes yes
Collector w/drainage sytem yes yes yes
Solvent level monitoring yes yes yes
RFID yes yes yes
Fume Encloser yes yes yes

Size
W: 15.75" - 40cm W: 15.75" - 40cm W: 15.75" - 40cm
D: 20.0" - 51cm D: 20.0" - 51cm D: 20.0" - 51cm
H: 29.5" - 75cm H: 29.5" - 75cm H: 29.5" - 75cm

18 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 Peptides Purification Small Organic molecules
Impurities Identification
Natural Products
Peptides, Oligonucleotides
Proteins, Bio-drugs
Metabolites Isolation
Traces Enrichment

Accuracy Purification of complex mixtures of peptides

125
& Repeatability
Maximum efficiency.
up to mL/min The use of small particle size combined with the reduction of dead volume makes it possible to discriminate
60mm i.d. nearby peptides in terms of amino acid sequence.
Col.
250 InterSoft® X
bar
“Genius” Artificial Intelligence embeded
User-friendly
Click & Drag Gradient
Intelligent Isocratic Mode

Unit Control
15" touch screen Solvent tray w/drainage system
Remote Control
USB x8 - RJ45

Pre-cols holder

Quaternary Gradient Pump

Air purge
Washing Disc + Pump

Injection
Fraction Collector Liquid or Dry-Load injection
3 racks Gen5 w/column equilibration.
132 tubes 18x150mm
Drainage system Load & Gotm technology:
an automated
6 port electric valve
+ 10 port electric valve
manages solid injection

Column i.d. up to
=> 60mm

Modular & Easy to Maintain

Built to last

Detection
PF-5.250P: UV: 200-400nm
PF-5.250P + Pack-UVextended: UV: 200-800nm
(Multi wavelength & Scan collection
Spectral view & Purity confirmation)
Integrated cols holder
Flow Cell: 1.3mm / 55µL

Load Boost
& Go & Go P/N: PFG5Q0

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 19

 Small Organic molecules
Impurities Identification
Natural Products
Peptides, Oligonucleotides
UP3 Proteins, Bio-drugs
Metabolites Isolation
Traces Enrichment

Accuracy UP3 - Ultra Performance Proteins Purification

100
& Repeatability
Open new horizons in method development and purification of proteins.
up to mL/min A single user-friendly instrument that provides more capabilities and flexibility, saving time. Compatible with
50mm i.d. 100% of existing SEC, IEX, affinity low and medium pressure bio-columns.
Col.
50
bar
InterSoft® X
“Genius” Artificial Intelligence embeded
User-friendly
Click & Drag Gradient
Intelligent Isocratic Mode

Unit Control
15" touch screen
Remote Control
USB x8 - RJ45

Solvent tray w/drainage system

Integrated column
holder

Quaternary Gradient Pump

Washing ( pump + discs)
Back flush valve

Fraction Collector
96 well plates up to bottles
Drainage system
Fume enclosure

Column i.d. up to
=> 50mm

Modular & Easy to Maintain

Built to last
Injection
Liquid injection
w/column equilibration
or via external pump.
Detection
UV: 250 or 280nm Load & Gotm technology
pH meter: 0.14 through an automated
Conductimeter: 0.01 - 800 mS/cm 6 ports electric valves
RI manages liquid & solid injection

20 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 Peptides & Oligonucleotides Peptides & Oligonucleotides Proteins Purification
PF-5.125P PF-5.250P UP3
Pump
Flow rate 250mL/min 125mL/min 100mL/min
Pressure max. 125bar 250bar 50 bar
Quaternary Gradient yes yes yes
Air purge yes yes no
Washing discs yes yes yes
Pump washing discs (pack) yes yes yes

Detector
254 nm & 280 nm
UV: 200 - 400nm multi wavelength & scan collection yes yes
200-to-400 nm as an option*
UV: 200 - 800nm multi wavelength & scan collection pack-UVextended pack-UVextended no
Spectral view & purity confirmation yes yes *pack-UV
iELSD Detection (pack) no
pH/Conductimeter no no yes

Injection
4 port electrical valve no no no
6 port electrical valve w/loop no no yes
6 port + 10 port electrical valves w/loop yes yes no
Injection mode: liquid - Dry-load yes yes no

Column Selection valve

14 port /6 position electrical valve no no no
6 port electrical valve pack-Multi pack-Multi no

System Optimization
Tubings 1/8'' x 1.0mm id peek 1/16'' x 0.75mm id n.c.
Flow cell-optical length 1.3mm / 55µL 1.3mm/55µL n.c.

Columns Holder
Integrated yes yes yes
Pre-column holder yes yes no

Fraction Collector
3 racks Gen5 3 racks Gen5 3 racks Gen5
Regular Collector
132 tubes 18x150mm 132 tubes 18x150mm 132 tubes 18x150mm

Unit control
Touch screen 15" yes yes yes
USB 8 8 8
RJ45 yes yes yes

Software
InterSoft® X yes yes yes

Safety
Leak detection (pump, FC, …) yes yes yes
Solvent tray w/drainage system yes yes yes
Collector w/drainage sytem yes yes yes
Solvent level monitoring yes yes yes
RFID yes yes yes
Fume Encloser yes yes yes

Size
W: 15.75" - 40cm W: 15.75" - 40cm W: 15.75" - 40.0cm
D: 20.0" - 51cm D: 20.0" - 51cm D: 20" - 51.0cm
H: 29.5" - 75cm H: 29.5" - 75cm H: 29.5" - 75.0cm

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 21

 Interchim® puriFlash® MS
Mass triggered fraction collection for NP & RP Flash Purification & prep-LC
Unique Interchim® design Dynamic split & dilution:
- High-speed work with all columns sizes without generating backpressure
- Integrated post-split dilution to adjust the concentrations used in the MS source (no concentration limit - no signal saturation)
- Normal & Reverse Phase
- Intelligent Pilot of the puriFlash® system
- Normalized Scale signals MS, UV, ELSD (6 acquisition signals)

Compact Single Quad - APCI

Real-time reaction monitoring of batch
reactions.
The hood-based puriFlash MS reduces the
burden on open-access platforms.

puriFlash® MS (1200 m/z): 1H5460

puriFlash® MS (2000 m/z): 1G6770

puriFlash® MS puriFlash® MS-HMW

for small molecule, synthetic organic chemists. for large molecules - peptide synthesis, polymer chemistry
Upgraded specs for pos/neg switching, faster scanning & & natural products
higher flow rate
Sources : APCI - (ESI option) APCI or ESI
orthogonal ion sampling from heated capillary orthogonal ion sampling from heated capillary allows for small
Patented API:
allows for small single turbo pump. single turbo pump.
Positive/Negative Ionization Simultaneous Analysis Simultaneous Analysis
Flow rate range ESI 10µL/min - 1mL/min 10µL/min - 1mL/min
Flow rate range APCI 10µL/min - 2mL/min 10µL/min - 2mL/min
mass range (m/z) 10 to 1200 10 to 2000
Scan rate (m/z-units per second) 10000 10000
Resolution (m/z-unites FWHM) 0.5 - 0.7 0.5 - 0.7
Sensitivity (SIM - S/N de 10 pg Reserpine, 100:1 100:1
FIA 5µl injection à 100µl/min)
Accuracy (m/z) 0.1 0.1
Stability 0.1 0.1
(m/z-units per 24 hour period: 18 - 24 °C)

22 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 (U)HPLC &
Software Solutions
Integrated (U)HPLC and streamlined software solutions

Advion’s range of AVANT high

performance and ultra-high
performance liquid chromatography
systems can be used standalone with UV and UV/Vis detector
options, or with the expression CMS to provide seamlessly
integrated LC/CMS under the full control of Advion’s simple, intuitive
Mass Express software suite. The Advion AVANT series offers:

- HPLC and UHPLC binary pumps

- Automated solvent selection of 4 solvents
- 6-way column switching
- Divert valve
- UV-Vis DAD
- Column oven
- Autosamplers with optional cooling
- Modular and stackable design
- High-pressure mixing with optional degassing

Advion Software Features

Advion offers a range of proprietary software that makes utilizing and
interfacing with the expression CMS even easier.

Software packages include:

- Mass Express: Advion’s standard software that provides answers

in the fewest steps and shortest period of time possible in an easy-
to-use interface.

- Peak Express: Unique, patented data analysis software that looks

beyond chemical noise and automatically detects even the smallest
peaks without knowing the m/z.

- CheMS: A simplified interface that allows the user to quickly select

the workflow and type of compound with automatic optimization, so a
user does not need to be an expert in mass spec to use the system.

- LC Integration: Advion offers a software solution to interface the

Peak Express CMS with other LC’s to take advantage of existing instruments.

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 23

 The expression CMS Family of Compact Mass Spectrometers

The family of expression CMS was developed

with maximum versatility in mind allowing users to
switch rapidly between various sample introduction
techniques with little sample preparation. The
affordability, small size, and ease of use make
them ideal for a variety of applications including
reaction monitoring, teaching, cannabis/hemp,
food/ingredients, environmental and more.

Most laboratories require the ability to analyze a

range of samples using more than one technique
for more complete identification and
characterization of samples. The expression CMS
integrates with nearly every chemistry technique
from the simplest, fastest direct probe analysis
requiring no sample preparation to ultra-high
performance compound separation with LC
integration. The expression CMS offers the most
versatile sample techniques on the market.

Advion provides an extensive range of innovative

sample introduction systems that are fully integrated
with the expression CMS to provide solutions for all
the chemist's needs.

These techniques include:

- Plate Express® TLC Plate Reader
- Atmospheric Solids Analysis Probe (ASAP®)
- InertAtmospheric Solids Analysis Probe (iASAP)
- Touch Express™ Open Port Sampling Inteface
(OPSI)
- Volatile APCI (vAPCl)
- AVANT™ Modular (U)HPLC
- Direct Injection/Flow Injection Analysis (FIA)

The CMS also directly interfaces with:

- Flow Chemistry
- Flash Chromatography
- Preparative Liquid Chromatography (Prep-LC)
- Supercritical Fluids Chromatography (SFC)

24 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 APPLICATIONS
Unrivaled utility and flexibility for a wide range of applications

Reaction Monitoring
With the CMS, users can monitor progress and side reactions, and
can quickly determine the optimal time to quench a reaction mixture.

Teaching Chemistry
The system is available with a teaching package with curriculum
including video lectures and PowerPoint slides, offering professors
the ability to easily provide students with industry-related knowledge
and experience.

Cannabis & Hemp

Easy cannabinoid analysis, ideal for characterization, potency
testing, screening for contaminants such as pesticides, and
determination of optimal harvest time.

Food Safety and Ingredients Analysis

The CMS provides characterization, screening and purity
determination.

Environmental Analysis
The perfect environmental testing companion, the CMS offers fast,
direct screening and can be deployed in a field setting for immediate,
on-site answers.

Clinical Diagnostics
The CMS with the AVANT (U)HPLC is optimized for clinical LC/MS
assays such as Vitamin D2/D3 analysis or can be used standalone
with a variety of prep-free screening techniques.

Proteins and Peptides

The expression® CMS extended range mass spectrometer is ideal for
large molecule synthesis analysis including proteins, peptides, and
oligonucleotides

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 25

 The industry’s widest range of sample introduction techniques

Thin-layer chromatography (TLC) is a

routine, reliable method used in most
chemistry laboratories, but traditional
visualization techniques give no
structural information on the TLC
spots. Mass Spectrometry is a powerful technique for identifying
spots, but traditional, manual steps involving scraping spots from
the plate, extraction in suitable solvents and finally reconstitution in
solvent appropriate for injection into the mass spectrometer (MS),
make obtaining spectra difficult and time consuming. The Plate
Express is a device that provides a simple, automated means of
obtaining mass spectra directly from TLC plates.

With the Advion Plate Express you can:

- Identify spots in <1 minute
- Avoid the risk of overloading the mass spectrometer
- TLC spots contain the ideal amount of sample for mass
spectrometry
- Simplify the process of obtaining spectra
- Ideal for multi-user labs
- Software controlled - spectra obtained within a few mouse clicks
- No scraping TLC spots

ASAP Atmospheric Solids Analysis Probe

The direct atmospheric solids analysis probe, otherwise known as
ASAP, permits chemists fast and reliable sample analysis of liquid
and solid samples such as reaction mixtures, food samples, natural
products and tablets.

The Advion ASAP probe offers:

- No sample preparation
- No chromatography
- Fast and affordable sample analysis
- Solvent-free APCI
- Green practices without the need to buy and dispose of solvent
- lonizes a wider range of compounds

iASAP Inert ASAP Probe

The inert ASAP (iASAP) is a modification of the ASAP technique,
allowing easy sampling of air-sensitive compounds, such as metal
catalysts and organometallics, from reactions that are carried out in
a glove box or Schlenk line to prevent oxidation.

26 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

Easy, prep-free sampling for a range of liquid and solid samples

OPSI Open Port Sampling Interface

The Touch Express Open Port Sampling Interface (OPSI), is
designed for simple sampling of solids, liquids and sample
preparation tips and fibers. Any soluble sample touching the port is
analyzed by the mass spectrometer in mere seconds.

The OPSI source is a unique, prep-free sample technique that

offers:
- Compound ID and impurity detection from almost any surface
- Direct assays from sample preparation tips and SPME fibers
- Easy screening applications for drug research, food safety,
environmental, forensics
- Large molecule applications including proteins, peptides,
oligonucleotides and polymers

vAPCI Volatile APCI Source

vAPCl combined with the expression CMS is a fast and easy
method to quickly and confidently analyze compounds in air,
breath, headspace and other gas samples without the need for
additional sample preparation. vAPCl provides a sample inlet
linked by a heated transfer line to the APCI ion source of the CMS,
where the sample is ionized by chemical reaction by a corona
discharge.

vAPCl enables chemists to:

- Analyze samples directly in the gas phase
- Solvent-free APCI allows a greater range of compounds to be
ionized compared to traditional APCI ion sources.

Direct Injection &

FIA Flow Injection Analysis
Simple, direct injection valve offers users the ability to quickly and
easily analyze reaction mixtures by direct injection/flow injection
analysis. Both the APCI and ESI sources can receive samples for
flow injection analysis.

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 27

 Interchim® preparative integrated ELSD
Secure your purification by a Universal Detector
- Even non chromophore are now visible
- Specifically developed for purification mass response detector
- Full control of the split and the inlet flow
- True purification design nebulizer: no clogging
- Large dynamic range: mg up to hundred g
- Easy access /easy maintenance

Optimized Low Temperature Technology

Quicker droplets drying at lower temperature
Preserves the integrity of termo-sensitive compounds

Low Temperature Technology:

This technology provides greater sensitivity with both the nebulizer that enables droplet selection
and an effective photomultiplier.
With this patented technology nebulizer, the droplets dry faster at low temperatures, providing
appropriate signal intensities for semi-volatile compounds. This technology requires no additional
peripherals, such as a nebulizer with heating system (spray chamber) or an evaporation tube
(Peltier cooling) that can degrade the heat-sensitive compounds.
Dynamic Gain SAGA:
SAGA adapts the gain to avoid saturation while continuing to detect small quantities of products. ELSD becomes unsaturable without
impact on sensitivity.

Now, with Interchim SAGA

- Detection: high sensitivity photodiode

- Source: LED (470nm)
- Ambient temperature to 100°C
- Dynamic Split: 40µL / min sample in DEDL
- Gas: 1-1.5L/min - 1bar

28 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 puriVap-6™ Interchim® Evaporator
Simple & Smart - 6 positions

- 6 channels to run up to 6 samples (independent or simultaneous)

- Sample volumes from 2mL up to 60mL
- PID temperature control with digital display up to 100°C
- Temperature increases quickly in the first minutes (up to 4°C/min)
- Dedicated nitrogen flow regulator control for each sample
- Nitrogen blow in combination of heating concentration mode
- Possibility to choose the best position of nitrogen needle to ensure the most efficient evaporation
AWZ7P0 - AWZ7Q0

AWZ7P0 - AWZ7Q0

- Anti-corrosive material (PTFE)

- “Open view” to control sample volume level during the evaporation process
AWZ7M0

- Dry aluminium block heating mode to avoid potential water vapor interference
- Small difference temperature between channels (RSD: 0.11%)
- Low gas consumption (nitrogen gas supply : 1-2 bar max l flow rate: 7-8L/min)

Anti-corrosive material
Nitrogen blow in combination (PTFE)
of heating concentration mode
Possibility to choose the best
position of nitrogen needle
to ensure the most efficient PID temperature control
evaporation with digital display
Up to 100°C
Possibility to run up to Temperature increases quickly
6 samples in the first minutes (up to 4°C/min)
Parallel concentration model

Dry aluminium block

heating mode to avoid
potential water vapor
interference
Small temperature 6 channels
difference between (independent or
channels (RSD: 0.11%) simultaneous)
Dedicated nitrogen
flow regulator
control for each
sample

“Open view” to control sample volume level

during the evaporation process

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 29

 From theory to Modern Transfer TLC to Flash & Prep Chromatography
Basic principle:
The sample is dropped off, with a capillary, on the deposit line of the TLC plate which is then immersed in the tank containing the mobile
phase. It then travels through the stationary phase by capillary, carrying each compound which moves at its own velocity behind the solvent
front according to its affinity for the stationary and the mobile phases.

Solvent front
in progress
HPLC
Syringe
(V)

Deposit line
of the TLC
(L)

Sample
Vapor Phase Liquid Phase

In TLC, the “retention factor” (Rf) is defined by the ratio of the distance traveled by the analyte (da) over the distance traveled by the solvent
front (dS).

Solvent Front

Rfa = da / dS
Rfb = db / dS
ds
In practice, it is necessary to consider the amount of mobile phase necessary to use to elute the
solute out of the column. Taking into account their different geometries, this retention volume is
expressed relative to the void volume of the column used.
da It is aRf
dimensionless
a = da / ds number identified by the acronym CV (also called Vs)
Rfa
Rfb = db / ds
Vsa = CVa = 1/Rfa = 1 + ka
Vsb = CVb = 1/Rfb = 1 + kb
Rfb => ΔCV = CVb – CVa
There is a mathematical relation between CV and the retention factor k in liquid HPLC =>
db k = Ktr x(1/Rf – 1) et avec Ktr = cste = 1
=> Δk = Ktr x[(1/Rfb – 1) - (1/Rfa – 1)]
=> CV = Δk
Deposit line

30 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

Revolutionize your TLC using our App: “TLC to Flash & Prep Chromatography”

Augmented reality for a greatly improved daily

routine.
In our capacity as experts for purification, we aim to offer maximum
working comfort as well as the highest efficiency in your laboratory.
Thanks to the app that we have developed, we can share your future
vision by constantly providing new technology solutions. It takes a
digital form here, virtual but already real on the screen of your mobile
or tablet.

A unique technology that slides through your fingers with one single click.
Our idea is to support you and to save you time day by day. Therefore our App allows for:
- Our goal is to support you and to save you time day by day. Therefore, our app allows for:
- Automatic detection of your compounds and calculation of your Rf and ΔCV (=ΔK) values
- Direct (and secure) information transfer to puriFlash® and the “Genius” software, which suggests the best method for a successful
purification
- Data archiving, if desired.

Download the app now: bit.ly/interchimTLC

Picto
Picto
page
page
Picto
appli
appli
page
Picto
appli
page
Picto
appli
page appli
Picto page appli

The application is extremely intuitively and user-friendly:

Rf Rf Rf Rf Rf Rf

∆CV
∆CV ∆CV ∆CV ∆CV ∆CV

Take a picture Your compounds The application From the smallest Indicate the Save your TLC plate information.
of the TLC with will be detected calculates Rf and ΔCV obtained on solvents, their Send them to the email address
your smartphone automatically. ΔCV (= ΔK). It your TLC plate, proportions and of your choice or directly to your
or download it Select the ones indicates if the Rf are the application your comments puriFlash® by bluetooth or wifi: the
from your library. of interest with placed in the comfort gives you the level in the dedicated “Genius” software will recommend
a tap. zone to carry out your of difficulty of the areas. the best method for a successfull
purification. separation. purification.

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 31

 From theory to Modern Transfer HPLC to Prep
Method transfer from HPLC to Purification
Calculation of the preparative conditions = >
a) Flow rate (F)
It must be adjusted by keeping the linear velocity constant
5µm, 250 x 4.6mm
between the analytical and the transferred preparative opt.F = 0.75mL/min
method, taking into account the particle size and the
geometry of the column.
id2
( )( )d
Fprep = Fana x 2prep x p ana
id ana dp prep
15µm, 250 x 30.0mm
F = 11.0mL/min
Example:
Fprep = 0.75 x
( )()
30.02
4.62
x
5
15
= 11.0mL/min

b) Injected volume (V)

It must be adapted according to the volume of the phases to
obtain the same chromatographic efficiencies.
5µm, 250 x 4.6mm
The injected volumes are usually higher than those at the Vinj = 5µL
analytical scale to increase the loading capacity. Under
overloading conditions, asymmetric peaks and a change in
retention time are observed.

Vprep = Vana x
( )( )
id2prep
id2ana
L
x prep
L ana
15µm, 250 x 30.0mm
Vinj = 213µL

Example:

Vprep = 5 x
( )( )
30.02
4.62
x
250
250
= 213µL

c) Gradient conditions, isocratic step

It is necessary to keep the ratio isocratic time / dead time of
the column constant in analytic / preparative to keep a number 5µm, 250 x 4.6mm
of percolated column volumes equivalent. opt.F = 0.75mL/min

Tprep = Tana x
( )( )( )
id2prep
id2ana
L F
x prep x ana
L ana F prep
15µm, 250 x 30.0mm
T = time of the isocratic step F = 11.0mL/min

Example:
Tprep = 3 x
( )( )( )
30.02
4.62
x
250
250
x
0.75
11
= 11.0mL/min
100% Prep Gradient

50%

3 min step 8.7 min step

0%

32 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

HPLC analysis using standard gradient method

Flow: 1mL/min
A: Water +0.1% F. Acid
B: MeOH +0.1% F. Acid
Column: Interchim® 4.6mm x 250mm C18HQ 15µm
Sample conc.: 2mg/mL for each standard
Injection Volume: 3µL (6µg loaded on column)

Gradient transfer method*

UPFP-APCI/MS

Purge

Method

+
Sample
Queue
Flow: 20mL/min
A: Water +0.1% F. Acid
B: MeOH +0.1% F. Acid
Live run

Reprocess
Column: puriflash® Column C18HQ 15µm 35g
Sample conc.: 3mg/mL for each standard
Injection Volume: 500µL (1.5mg loaded on column)
Make-up flow: 500µL/min
Eluent make-up flow: MeOH +0.1% F. Acid
Admin

Settings

Orange trace: UV (Scan 200-600nm)

Red trace: TIC

Log in

Log out

Off

Solvent Theophylline Papaverine Quercetin Piperine

Original method (1) Transferred method (2)

Original column geometry Original exp. Conditions Transferred column geometry Transferred exp. Conditions
Column length (L1) 250mm Flow-rate (F1) 1000µL/min Column length (L1) 250mm Flow-rate (F1) 21240 µL/min
Column diameter (dc1) 4.6mm Inj. volume (Vinj.1) 20.0µL Column diameter (dc1) 21.2mm Inj. volume (Vinj.1) 424.8 µL
Particle size (dp1) 10.0µm 120 Particle size (dp1) 10.0µm 21240µL/min

100 120
Dwell volume (Vc1) 1.0mL 80
Dwell volume (Vc1) 25,0mL 100
21000µL/min

60 80
%B

60
%B

40
Additional gradient information 20
Additional gradient information 40
Suggested 0 Suggested 20
reconditionning step 29min 0,0 20.0 40.0 60.0 80.0 reconditionning step 29 min 0
0.0 20.0 40.0 60.0 80.0
time (minutes) time (minutes)
Original gradient profile Geometric transfer of the gradient profile
Step time (min) %A %B Step time (min) %A %B
initial conditions 0.00 60 40 initial conditions 0.00 60 40
initial hold 20.00 25 75 initial hold 19.82 25 75
3 30.00 25 75 3 29.82 25 75
4 40.00 0 100 4 39.82 0 100
5 70.00 0 100 5 69.82 0 100
Complex Purifications with Confidence
250mL/min@up to 125bar
* Davy Guillarme, Eur. J. Pharma. and Biopharma. 2008 , 68 , 430

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 33

 The Essential Stationary Phases for Purification
Normal Phase

puriFlash® IR-SI puriFlash® SI-HP puriFlash® SI-HC

60Å - 450m /g2
60Å - 500m /g2
60Å - 680m2/g
20 & 40/63µm 5, 10, 15, 30 & 50µm 15, 25 & 50µm
pH stability: 1.5 to 6.5 pH stability: 1.5 to 6.5 pH stability: 1.5 to 6.5
Economical High efficiency Greater loading capacity & productivity
Low back pressure

Normal Phase / Ion exchange Reverse Phase

puriFlash® NH2 puriFlash® RP-AQ puriFlash® C18-AQ

100Å - 300m /g 2
60Å - 500m2/g 100Å - 300m2/g
5, 10, 15, 30 & 50 µm 15 & 30µm 5, 10, 15 & 30µm
Amino RP-alkyl C18 Mono-functional
%C: 4 %C: 6 %C: 14
End-capping: One-step End-capping: Mixte End-capping: Mixte
pH stability: 2 to 6.5 pH stability: 2.0 to 7.5 pH stability: 2.0 to 7.5
Can be both a weak anion exchanger for strong acids The bonding chemistry makes it possible to start the The bonding chemistry makes it possible to start the
or a polar phase that can interact with the OH, NH, SH... gradient at a 100% water. Suitable for the separation gradient at a 100% water
functions. and purification of strongly and moderately polar Suitable for the separation and purification of
molecules. Compared to a C18, the peaks are eluted moderately polar and non-polar molecules.
earlier from the beginning of the gradient.

puriFlash® C18-HP Uptisphere® Strategy™ C18-HQ puriFlash® C18-XS

100Å - 300m2/g 100Å - 425m2/g 100Å - 300m2/g
5, 10, 15, 30 & 50µm 1.7, 2.2, 3, 5, 10, 15µm 5, 10, 15 & 30µm
C18 Mono-functional C18 Mono-functional C18 Mono-functional
%C: 16,5 %C: 19 %C: 17
End-capping: One-step End-capping: Multi-step End-capping: Multi-step
pH stability: 1.5 to 7.5 pH stability: 1.0 to 10.0 pH stability: 1 to 10.0
Suitable for multiple pharmaceutical applications. Suitable for many pharmaceutical applications and The proprietary end-capping multi-step technology
This is an excellent choice for routine reverse phase routine methods. ensures stability under high pH conditions, up to 10.
purifications. Its specific surface area of 425m2/g It is an excellent phase for the complete separation of
gives it a high loading capacity. basic molecules.

Complete list on www.flash-chromatography.com

34 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com
 puriFlash® Columns
Interchim® Flash columns
Interchim® offers more than 35 selections for the purification of small & bio-molecules.
Our Ultra Pure Spherical Silica combined with our chemistry establishes optimized sample recovery and makes easier transfer from
analytical to purification.
Interchim® Genius allows an easy transfer from any TLC or analytical data from any columns to “ready-to-go” and safe
Gradient Purification Method
RFID Traceability

Selectivity / Purity _> Capacity _> Productivity

Code F0001 F0004 F0012 F0025 F0040 F0080 F0120 F0220 F0330 F0800 F1600
Ø int. (mm) 9 12 21 21 27 31 36 60 60 78 104
L (mm) 26 68 84 133 135 205 224 153 226 341 385
CV0 (mL) 1.2 5 19 32 48 102 153 269 405 1 078 2 170
Flowrate - Typical (mL/min) 2.5 5 15 15 26 34 46 127 127 216 383
Flowrate - Range (mL/min) 1 - 10 5 - 20 15 - 50 15 - 50 20 - 70 30 -100 40 - 150 80 - 300 80 - 300 150 - 300 200 - 500

Interchim® LC Preparative columns

Interchim® Preparative columns range from 10.0 to 50.8mm i.d and are for the purification of samples ranging from 0.5mg to 1 gram.
Column tubing & column packing Sample dispersion
The tube polishing value (Ra) has a fundamental importance in preparative The loading of sample onto a preparative column requires stringent
chromatography. management to establish quality separations. Column overloading results
A primary reason for broad peaks and low efficiency is the utilization in a poor retention of pure fraction and therefore particular attention
of poor quality tubing. Molecules in the center of the mobile phase needs to be placed upon selecting the appropriate column dimension
stream can move more rapidly than molecules closer to the side due to and the properties of the stationary phase. In addition, careful sample
friction against the tubing surface. introduction control
The lower the Ra value, the smoother the surface is, and the less ‘drag’ the to the column is necessary to establish a homogeneous sample dispersion
tubing will place upon a given separation. Modulo-cart preparative columns through the sorbent bead head. Sample typically enters a preparative
pay particular attention to this potential negative phenomenon. All columns column through a 1/16” fitting; poor sample loading will lead to overloading
have extremely smooth internal surfaces (typically 8μ inch of Ra) to certain areas of the stationary phase whilst other areas will be underloaded.
considerably reduce issues of drag and maintain column efficiency. E.g. For a 50mm i.d column with a 500μm i.d capillary fitting - sample
Efficiency is also managed through Interchim®’s state-of-the art introduced to the column (without any sample distributor) will only interact
proprietary packing processes - Modulo-cart Prep can with 0.01% of the surface column head. In addition to a dramatic loss
withstand packing pressures up to 550bar contributing in capacity there will also be a high potential for the column head to
strongly to a good bed stability and column life. prematurely clog, rapidly reducing column life.
To prevent this problem, Interchim®’s Modulo-cart Preparative columns are
outfitted with a sample distributor. The sample distributor design maximizes
the efficiency of sample volume dispersion and the sample mass introduced
to the surface of the column head extending column life.

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 35

 puriFlash® Columns
puriFlash® IR-SI
Flash Columns 20µm RFID Qty 50µm RFID Qty
F0004 IR-20SI-F0004 -R 40u IR-50SI-F0004 -R 40u
F0012 IR-20SI-F0012 -R 30u IR-50SI-F0012 -R 30u
F0025 IR-20SI-F0025 -R 25u IR-50SI-F0025 -R 25u
F0040 IR-20SI-F0040 -R 20u IR-50SI-F0040 -R 20u
F0080 IR-20SI-F0080 -R 10u IR-50SI-F0080 -R 10u
F0120
F0220
IR-20SI-F0120
IR-20SI-F0220
-R
-R
8u
4u
IR-50SI-F0120
IR-50SI-F0220
-R
-R
8u
4u
RFID Columns
F0330 IR-20SI-F0330 -R 4u IR-50SI-F0330 -R 4u
add [-R] at the end of P/N
F0800 IR-20SI-F0800 -R 1u IR-50SI-F0800 -R 1u
F1600 IR-20SI-F1600 -R 1u IR-50SI-F1600 -R 1u

puriFlash® SIHP
LC Preparative Columns 5µm RFID Qty 10µm RFID Qty 15µm RFID Qty
250 x 4.6mm PF5SIHP-250/P46 -R 1u PF10SIHP-250/P46 -R 1u PF15SIHP-250/P46 -R 1u
150 x 10.0mm PF5SIHP-150/100 -R 1u PF10SIHP-150/100 -R 1u PF15SIHP-150/100 -R 1u
250 x 10.0mm PF5SIHP-250/100 -R 1u PF10SIHP-250/100 -R 1u PF15SIHP-250/100 -R 1u
50 x 21.2mm PF5SIHP-050/212 -R 1u PF10SIHP-050/212 -R 1u PF15SIHP-050/212 -R 1u
100 x 21.2mm PF5SIHP-100/212 -R 1u PF10SIHP-100/212 -R 1u PF15SIHP-100/212 -R 1u
150 x 21.2mm PF5SIHP-150/212 -R 1u PF10SIHP-150/212 -R 1u PF15SIHP-150/212 -R 1u
250 x 21.2mm PF5SIHP-250/212 -R 1u PF10SIHP-250/212 -R 1u PF15SIHP-250/212 -R 1u
50 x 30.0mm PF5SIHP-050/300 -R 1u PF10SIHP-050/300 -R 1u PF15SIHP-050/300 -R 1u
100 x 30.0mm PF5SIHP-100/300 -R 1u PF10SIHP-100/300 -R 1u PF15SIHP-100/300 -R 1u
150 x 30.0mm PF5SIHP-150/300 -R 1u PF10SIHP-150/300 -R 1u PF15SIHP-150/300 -R 1u
250 x 30.0mm PF5SIHP-250/300 -R 1u PF10SIHP-250/300 -R 1u PF15SIHP-250/300 -R 1u
50 x 50.0mm PF5SIHP-050/500 -R 1u PF10SIHP-050/500 -R 1u PF15SIHP-050/500 -R 1u
250 x 50.0mm PF5SIHP-250/500 -R 1u PF10SIHP-250/500 -R 1u PF15SIHP-250/500 -R 1u

puriFlash® SIHP
Flash Columns 15µm RFID Qty 30µm RFID Qty 50µm RFID Qty
F0001 SC-15SIHP-F0001 -R 50u --- -R --- --- -R ---
F0004 PF-15SIHP-F0004 -R 20u PF-30SIHP-F0004 -R 40u PF-50SIHP-F0004 -R 40u
F0012 PF-15SIHP-F0012 -R 20u PF-30SIHP-F0012 -R 30u PF-50SIHP-F0012 -R 30u
F0025 PF-15SIHP-F0025 -R 12u PF-30SIHP-F0025 -R 25u PF-50SIHP-F0025 -R 25u
F0040 PF-15SIHP-F0040 -R 12u PF-30SIHP-F0040 -R 20u PF-50SIHP-F0040 -R 20u
F0080 PF-15SIHP-F0080 -R 4u PF-30SIHP-F0080 -R 10u PF-50SIHP-F0080 -R 10u
F0120 PF-15SIHP-F0120 -R 4u PF-30SIHP-F0120 -R 8u PF-50SIHP-F0120 -R 8u
F0220 PF-15SIHP-F0220 -R 2u PF-30SIHP-F0220 -R 4u PF-50SIHP-F0220 -R 4u
F0330 PF-15SIHP-F0330 -R 2u PF-30SIHP-F0330 -R 4u PF-50SIHP-F0330 -R 4u
F0800 --- --- --- PF-30SIHP-F0800 -R 1u PF-50SIHP-F0800 -R 1u
F1600 --- --- --- PF-30SIHP-F1600 -R 1u PF-50SIHP-F1600 -R 1u

puriFlash® SIHP - Jumbo

Flash Columns 15µm RFID Qty 30µm RFID Qty 50µm RFID Qty
F0004 PF-15SIHP-JP-F0004 -R 80 u PF-30SIHP-JP-F0004 -R 160u PF-50SIHP-JP-F0004 -R 160u
F0012 PF-15SIHP-JP-F0012 -R 80 u PF-30SIHP-JP-F0012 -R 120u PF-50SIHP-JP-F0012 -R 120u
F0025 PF-15SIHP-JP-F0025 -R 48 u PF-30SIHP-JP-F0025 -R 100u PF-50SIHP-JP-F0025 -R 100u
F0040 PF-15SIHP-JP-F0040 -R 48 u PF-30SIHP-JP-F0040 -R 80u PF-50SIHP-JP-F0040 -R 80u
F0080 PF-15SIHP-JP-F0080 -R 32 u PF-30SIHP-JP-F0080 -R 40u PF-50SIHP-JP-F0080 -R 40u
F0120 PF-15SIHP-JP-F0120 -R 32 u PF-30SIHP-JP-F0120 -R 32u PF-50SIHP-JP-F0120 -R 32u
F0220 PF-15SIHP-JP-F0220 -R 8u PF-30SIHP-JP-F0220 -R 16u PF-50SIHP-JP-F0220 -R 16u
F0330 PF-15SIHP-JP-F0330 -R 8u PF-30SIHP-JP-F0330 -R 16u PF-50SIHP-JP-F0330 -R 16u
F0800 --- --- --- PF-30SIHP-JP-F0800 -R 4u PF-50SIHP-JP-F0800 -R 4u
F1600 --- --- PF-30SIHP-JP-F1600 -R 4u PF-50SIHP-JP-F1600 -R 4u

36 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 puriFlash® Columns
puriFlash® SIHC
Flash Columns 15µm RFID Qty 25µm RFID Qty 50µm RFID Qty
F0001 SC-15SIHC-F0001 -R 50u --- --- --- --- --- ---
F0004 PF-15SIHC-F0004 -R 20u PF-25SIHC-F0004 -R 40u PF-50SIHC-F0004 -R 40u
F0012 PF-15SIHC-F0012 -R 20u PF-25SIHC-F0012 -R 30u PF-50SIHC-F0012 -R 30u
F0025 PF-15SIHC-F0025 -R 12u PF-25SIHC-F0025 -R 25u PF-50SIHC-F0025 -R 25u
F0040 PF-15SIHC-F0040 -R 12u PF-25SIHC-F0040 -R 20u PF-50SIHC-F0040 -R 20u
F0080 PF-15SIHC-F0080 -R 4u PF-25SIHC-F0080 -R 10u PF-50SIHC-F0080 -R 10u
F0120 PF-15SIHC-F0120 -R 4u PF-25SIHC-F0120 -R 8u PF-50SIHC-F0120 -R 8u
F0220 PF-15SIHC-F0220 -R 2u PF-25SIHC-F0220 -R 4u PF-50SIHC-F0220 -R 4u
F0330 PF-15SIHC-F0330 -R 2u PF-25SIHC-F0330 -R 4u PF-50SIHC-F0330 -R 4u
F0800 --- --- --- PF-25SIHC-F0800 -R 1u PF-50SIHC-F0800 -R 1u
F1600 --- --- --- PF-25SIHC-F1600 -R 1u PF-50SIHC-F1600 -R 1u

puriFlash® SIHC - Jumbo pack

Flash Columns 15µm RFID Qty 25µm RFID Qty 50µm RFID Qty
F0004 PF-15SIHC-JP-F0004 -R 80u PF-25SIHC-JP-F0004 -R 160u PF-50SIHC-JP-F0004 -R 160u
F0012 PF-15SIHC-JP-F0012 -R 80u PF-25SIHC-JP-F0012 -R 120u PF-50SIHC-JP-F0012 -R 120u
F0025 PF-15SIHC-JP-F0025 -R 48u PF-25SIHC-JP-F0025 -R 100u PF-50SIHC-JP-F0025 -R 100u
F0040 PF-15SIHC-JP-F0040 -R 48u PF-25SIHC-JP-F0040 -R 80u PF-50SIHC-JP-F0040 -R 80u
F0080 PF-15SIHC-JP-F0080 -R 16u PF-25SIHC-JP-F0080 -R 40u PF-50SIHC-JP-F0080 -R 40u
F0120 PF-15SIHC-JP-F0120 -R 16u PF-25SIHC-JP-F0120 -R 32u PF-50SIHC-JP-F0120 -R 32u
F0220 PF-15SIHC-JP-F0220 -R 8u PF-25SIHC-JP-F0220 -R 16u PF-50SIHC-JP-F0220 -R 16u
F0330 PF-15SIHC-JP-F0330 -R 8u PF-25SIHC-JP-F0330 -R 16u PF-50SIHC-JP-F0330 -R 16u
F0800 --- --- --- PF-25SIHC-JP-F0800 -R 4u PF-50SIHC-JP-F0800 -R 4u
F1600 --- --- --- PF-25SIHC-JP-F1600 -R 4u PF-50SIHC-JP-F1600 -R 4u

puriFlash® NH2
LC Preparative Columns 5µm RFID Qty 10µm RFID Qty 15µm RFID Qty
250 x 4.6mm PF5NH2-250/P46 -R 1u PF10NH2-250/P46 -R 1u PF15NH2-250/P46 -R 1u
150 x 10.0mm PF5NH2-150/100 -R 1u PF10NH2-150/100 -R 1u PF15NH2-150/100 -R 1u
250 x 10.0mm PF5NH2-250/100 -R 1u PF10NH2-250/100 -R 1u PF15NH2-250/100 -R 1u
50 x 21.2mm PF5NH2-050/212 -R 1u PF10NH2-050/212 -R 1u PF15NH2-050/212 -R 1u
100 x 21.2mm PF5NH2-100/212 -R 1u PF10NH2-100/212 -R 1u PF15NH2-100/212 -R 1u
150 x 21.2mm PF5NH2-150/212 -R 1u PF10NH2-150/212 -R 1u PF15NH2-150/212 -R 1u
250 x 21.2mm PF5NH2-250/212 -R 1u PF10NH2-250/212 -R 1u PF15NH2-250/212 -R 1u
50 x 30.0mm PF5NH2-050/300 -R 1u PF10NH2-050/300 -R 1u PF15NH2-050/300 -R 1u
100 x 30.0mm PF5NH2-100/300 -R 1u PF10NH2-100/300 -R 1u PF15NH2-100/300 -R 1u
150 x 30.0mm PF5NH2-150/300 -R 1u PF10NH2-150/300 -R 1u PF15NH2-150/300 -R 1u
250 x 30.0mm PF5NH2-250/300 -R 1u PF10NH2-250/300 -R 1u PF15NH2-250/300 -R 1u
50 x 50.0mm PF5NH2-050/500 -R 1u PF10NH2-050/500 -R 1u PF15NH2-050/500 -R 1u
250 x 50.0mm PF5NH2-250/500 -R 1u PF10NH2-250/500 -R 1u PF15NH2-250/500 -R 1u

Flash Columns 15µm RFID Qty 30µm RFID Qty 50µm RFID Qty
F0004 PF-15NH2-F0004 -R 4u PF-30NH2-F0004 -R 4u PF-50NH2-F0004 -R 4u
F0012 PF-15NH2-F0012 -R 2u PF-30NH2-F0012 -R 2u PF-50NH2-F0012 -R 2u
F0025 PF-15NH2-F0025 -R 1u PF-30NH2-F0025 -R 1u PF-50NH2-F0025 -R 1u
F0040 PF-15NH2-F0040 -R 1u PF-30NH2-F0040 -R 1u PF-50NH2-F0040 -R 1u
F0080 PF-15NH2-F0080 -R 1u PF-30NH2-F0080 -R 1u PF-50NH2-F0080 -R 1u
F0120 PF-15NH2-F0120 -R 1u PF-30NH2-F0120 -R 1u PF-50NH2-F0120 -R 1u
F0220 PF-15NH2-F0220 -R 1u PF-30NH2-F0220 -R 1u PF-50NH2-F0220 -R 1u
F0330 PF-15NH2-F0330 -R 1u PF-30NH2-F0330 -R 1u PF-50NH2-F0330 -R 1u
F0800 --- --- --- PF-30NH2-F0800 -R 1u PF-50NH2-F0800 -R 1u
F1600 --- --- --- PF-30NH2-F1600 -R 1u PF-50NH2-F1600 -R 1u

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 37

 puriFlash® Columns
puriFlash® NH2HC
Flash Columns 50µm RFID Qty
F0004 PF-50NH2HC-F0004 -R 4u
F0012 PF-50NH2HC-F0012 -R 2u
F0025 PF-50NH2HC-F0025 -R 1u
F0040 PF-50NH2HC-F0040 -R 1u
F0080 PF-50NH2HC-F0080 -R 1u RFID Columns
F0120 PF-50NH2HC-F0120 -R 1u add [-R] at the end of P/N
F0220 PF-50NH2HC-F0220 -R 1u
F0330 PF-50NH2HC-F0330 -R 1u
F0800 PF-50NH2HC-F0800 -R 1u
F1600 PF-50NH2HC-F1600 -R 1u

puriFlash® RP-AQ
LC Preparative Columns 15µm RFID Qty
250 x 4.6mm PF15RPAQ-250/P46 -R 1u
150 x 10.0mm PF15RPAQ-150/100 -R 1u
250 x 10.0mm PF15RPAQ-250/100 -R 1u
50 x 21.2mm PF15RPAQ-050/212 -R 1u
100 x 21.2mm PF15RPAQ-100/212 -R 1u
150 x 21.2mm
250 x 21.2mm
PF15RPAQ-150/212
PF15RPAQ-250/212
-R
-R
1u
1u
RFID Columns
add [-R] at the end of P/N
50 x 30.0mm PF15RPAQ-050/300 -R 1u
100 x 30.0mm PF15RPAQ-100/300 -R 1u
150 x 30.0mm PF15RPAQ-150/300 -R 1u
250 x 30.0mm PF15RPAQ-250/300 -R 1u
50 x 50.0mm PF15RPAQ-050/500 -R 1u
250 x 50.0mm PF15RPAQ-250/500 -R 1u

puriFlash® RP-AQ
Flash Columns 15µm RFID Qty 30µm RFID Qty
F0001 SC-15RPAQ-F0001 -R 25u --- -R ---
F0004 PF-15RPAQ-F0004 -R 4u PF-30RPAQ-F0004 -R 4u
F0012 PF-15RPAQ-F0012 -R 2u PF-30RPAQ-F0012 -R 2u
F0025 PF-15RPAQ-F0025 -R 1u PF-30RPAQ-F0025 -R 1u
F0040 PF-15RPAQ-F0040 -R 1u PF-30RPAQ-F0040 -R 1u
F0080 PF-15RPAQ-F0080 -R 1u PF-30RPAQ-F0080 -R 1u
F0120
F0220
PF-15RPAQ-F0120
PF-15RPAQ-F0220
-R
-R
1u
1u
PF-30RPAQ-F0120
PF-30RPAQ-F0220
-R
-R
1u
1u
RFID Columns
F0330 PF-15RPAQ-F0330 -R 1u PF-30RPAQ-F0330 -R 1u
add [-R] at the end of P/N
F0800 --- --- --- PF-30RPAQ-F0800 -R 1u
F1600 --- --- --- PF-30RPAQ-F1600 -R 1u

38 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 puriFlash® Columns
Uptisphere® Strategytm C18-HQ
LC Preparative Columns 5µm RFID Qty 10µm RFID Qty 15µm RFID Qty
250 x 4.6mm US5C18HQ-250/P46 -R 1u US10C18HQ-250/P46 -R 1u US15C18HQ-250/P46 -R 1u
150 x 10.0mm US5C18HQ-150/100 -R 1u US10C18HQ-150/100 -R 1u US15C18HQ-150/100 -R 1u
250 x 10.0mm US5C18HQ-250/100 -R 1u US10C18HQ-250/100 -R 1u US15C18HQ-250/100 -R 1u
50 x 21.2mm US5C18HQ-050/212 -R 1u US10C18HQ-050/212 -R 1u US15C18HQ-050/212 -R 1u
100 x 21.2mm US5C18HQ-100/212 -R 1u US10C18HQ-100/212 -R 1u US15C18HQ-100/212 -R 1u
150 x 21.2mm US5C18HQ-150/212 -R 1u US10C18HQ-150/212 -R 1u US15C18HQ-150/212 -R 1u
250 x 21.2mm US5C18HQ-250/212 -R 1u US10C18HQ-250/212 -R 1u US15C18HQ-250/212 -R 1u
50 x 30.0mm US5C18HQ-050/300 -R 1u US10C18HQ-050/300 -R 1u US15C18HQ-050/300 -R 1u
100 x 30.0mm US5C18HQ-100/300 -R 1u US10C18HQ-100/300 -R 1u US15C18HQ-100/300 -R 1u
150 x 30.0mm US5C18HQ-150/300 -R 1u US10C18HQ-150/300 -R 1u US15C18HQ-150/300 -R 1u
250 x 30.0mm US5C18HQ-250/300 -R 1u US10C18HQ-250/300 -R 1u US15C18HQ-250/300 -R 1u
50 x 50.0mm US5C18HQ-050/500 -R 1u US10C18HQ-050/500 -R 1u US15C18HQ-050/500 -R 1u
250 x 50.0mm US5C18HQ-250/500 -R 1u US10C18HQ-250/500 -R 1u US15C18HQ-250/500 -R 1u

Flash Columns 15µm RFID Qty

F0001 SC-15C18HQ-F0001 -R 25u
F0004 PF-15C18HQ-F0004 -R 4u
F0012 PF-15C18HQ-F0012 -R 2u
F0025
F0040
PF-15C18HQ-F0025
PF-15C18HQ-F0040
-R
-R
1u
1u
RFID Columns
F0080 PF-15C18HQ-F0080 -R 1u
add [-R] at the end of P/N
F0120 PF-15C18HQ-F0120 -R 1u
F0220 PF-15C18HQ-F0220 -R 1u
F0330 PF-15C18HQ-F0330 -R 1u

puriFlash® C18-XS
LC Preparative Columns 5µm RFID Qty 10µm RFID Qty 15µm RFID Qty
250 x 4.6mm PF5C18XS-250/P46 -R 1u PF10C18XS-250/P46 -R 1u PF15C18XS-250/P46 -R 1u
150 x 10.0mm PF5C18XS-150/100 -R 1u PF10C18XS-150/100 -R 1u PF15C18XS-150/100 -R 1u
250 x 10.0mm PF5C18XS-250/100 -R 1u PF10C18XS-250/100 -R 1u PF15C18XS-250/100 -R 1u
50 x 21.2mm PF5C18XS-050/212 -R 1u PF10C18XS-050/212 -R 1u PF15C18XS-050/212 -R 1u
100 x 21.2mm PF5C18XS-100/212 -R 1u PF10C18XS-100/212 -R 1u PF15C18XS-100/212 -R 1u
150 x 21.2mm PF5C18XS-150/212 -R 1u PF10C18XS-150/212 -R 1u PF15C18XS-150/212 -R 1u
250 x 21.2mm PF5C18XS-250/212 -R 1u PF10C18XS-250/212 -R 1u PF15C18XS-250/212 -R 1u
50 x 30.0mm PF5C18XS-050/300 -R 1u PF10C18XS-050/300 -R 1u PF15C18XS-050/300 -R 1u
100 x 30.0mm PF5C18XS-100/300 -R 1u PF10C18XS-100/300 -R 1u PF15C18XS-100/300 -R 1u
150 x 30.0mm PF5C18XS-150/300 -R 1u PF10C18XS-150/300 -R 1u PF15C18XS-150/300 -R 1u
250 x 30.0mm PF5C18XS-250/300 -R 1u PF10C18XS-250/300 -R 1u PF15C18XS-250/300 -R 1u
50 x 50.0mm PF5C18XS-050/500 -R 1u PF10C18XS-050/500 -R 1u PF15C18XS-050/500 -R 1u
250 x 50.0mm PF5C18XS-250/500 -R 1u PF10C18XS-250/500 -R 1u PF15C18XS-250/500 -R 1u

Flash Columns 15µm RFID Qty 30µm RFID Qty

F0001 SC-15C18XS-F0001 -R 25u --- --- ---
F0004 PF-15C18XS-F0004 -R 4u PF-30C18XS-F0004 -R 4u
F0012 PF-15C18XS-F0012 -R 2u PF-30C18XS-F0012 -R 2u
F0025 PF-15C18XS-F0025 -R 1u PF-30C18XS-F0025 -R 1u
F0040
F0080
PF-15C18XS-F0040
PF-15C18XS-F0080
-R
-R
1u
1u
PF-30C18XS-F0040
PF-30C18XS-F0080
-R
-R
1u
1u
RFID Columns
F0120 PF-15C18XS-F0120 -R 1u PF-30C18XS-F0120 -R 1u add [-R] at the end of P/N
F0220 PF-15C18XS-F0220 -R 1u PF-30C18XS-F0220 -R 1u
F0330 PF-15C18XS-F0330 -R 1u PF-30C18XS-F0330 -R 1u
F0800 --- --- --- PF-30C18XS-F0800 -R 1u
F1600 --- --- --- PF-30C18XS-F1600 -R 1u

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 39

 puriFlash® Columns
puriFlash® C18-HP
LC Preparative Columns 5µm RFID Qty 10µm RFID Qty 15µm RFID Qty
250 x 4.6mm PF5C18HP-250/P46 -R 1u PF10C18HP-250/P46 -R 1u PF15C18HP-250/P46 -R 1u
150 x 10.0mm PF5C18HP-150/100 -R 1u PF10C18HP-150/100 -R 1u PF15C18HP-150/100 -R 1u
250 x 10.0mm PF5C18HP-250/100 -R 1u PF10C18HP-250/100 -R 1u PF15C18HP-250/100 -R 1u
50 x 21.2mm PF5C18HP-050/212 -R 1u PF10C18HP-050/212 -R 1u PF15C18HP-050/212 -R 1u
100 x 21.2mm PF5C18HP-100/212 -R 1u PF10C18HP-100/212 -R 1u PF15C18HP-100/212 -R 1u
150 x 21.2mm PF5C18HP-150/212 -R 1u PF10C18HP-150/212 -R 1u PF15C18HP-150/212 -R 1u
250 x 21.2mm PF5C18HP-250/212 -R 1u PF10C18HP-250/212 -R 1u PF15C18HP-250/212 -R 1u
50 x 30.0mm PF5C18HP-050/300 -R 1u PF10C18HP-050/300 -R 1u PF15C18HP-050/300 -R 1u
100 x 30.0mm PF5C18HP-100/300 -R 1u PF10C18HP-100/300 -R 1u PF15C18HP-100/300 -R 1u
150 x 30.0mm PF5C18HP-150/300 -R 1u PF10C18HP-150/300 -R 1u PF15C18HP-150/300 -R 1u
250 x 30.0mm PF5C18HP-250/300 -R 1u PF10C18HP-250/300 -R 1u PF15C18HP-250/300 -R 1u
50 x 50.0mm PF5C18HP-050/500 -R 1u PF10C18HP-050/500 -R 1u PF15C18HP-050/500 -R 1u
250 x 50.0mm PF5C18HP-250/500 -R 1u PF10C18HP-250/500 -R 1u PF15C18HP-250/500 -R 1u

Flash Columns 15µm RFID Qty 30µm RFID Qty 50µm RFID Qty
F0001 SC-15C18HP-F0001 -R 25u --- -R --- --- -R ---
F0004 PF-15C18HP-F0004 -R 4u PF-30C18HP-F0004 -R 25u PF-50C18HP-F0004 -R 25u
F0012 PF-15C18HP-F0012 -R 2u PF-30C18HP-F0012 -R 4u PF-50C18HP-F0012 -R 4u
F0025 PF-15C18HP-F0025 -R 1u PF-30C18HP-F0025 -R 2u PF-50C18HP-F0025 -R 2u
F0040 PF-15C18HP-F0040 -R 1u PF-30C18HP-F0040 -R 1u PF-50C18HP-F0040 -R 1u
F0080 PF-15C18HP-F0080 -R 1u PF-30C18HP-F0080 -R 1u PF-50C18HP-F0080 -R 1u
F0120 PF-15C18HP-F0120 -R 1u PF-30C18HP-F0120 -R 1u PF-50C18HP-F0120 -R 1u
F0220 PF-15C18HP-F0220 -R 1u PF-30C18HP-F0220 -R 1u PF-50C18HP-F0220 -R 1u
F0330 PF-15C18HP-F0330 -R 1u PF-30C18HP-F0330 -R 1u PF-50C18HP-F0330 -R 1u
F0800 --- --- --- PF-30C18HP-F0800 -R 1u PF-50C18HP-F0800 -R 1u
F1600 --- --- --- PF-30C18HP-F1600 -R 1u PF-50C18HP-F1600 -R 1u

puriFlash® C18-AQ
LC Preparative Columns 5µm RFID Qty 10µm RFID Qty 15µm RFID Qty
250 x 4.6mm PF5C18AQ-250/P46 -R 1u PF10C18AQ-250/P46 -R 1u PF15C18AQ-250/P46 -R 1u
150 x 10.0mm PF5C18AQ-150/100 -R 1u PF10C18AQ-150/100 -R 1u PF15C18AQ-150/100 -R 1u
250 x 10.0mm PF5C18AQ-250/100 -R 1u PF10C18AQ-250/100 -R 1u PF15C18AQ-250/100 -R 1u
50 x 21.2mm PF5C18AQ-050/212 -R 1u PF10C18AQ-050/212 -R 1u PF15C18AQ-050/212 -R 1u
100 x 21.2mm PF5C18AQ-100/212 -R 1u PF10C18AQ-100/212 -R 1u PF15C18AQ-100/212 -R 1u
150 x 21.2mm PF5C18AQ-150/212 -R 1u PF10C18AQ-150/212 -R 1u PF15C18AQ-150/212 -R 1u
250 x 21.2mm PF5C18AQ-250/212 -R 1u PF10C18AQ-250/212 -R 1u PF15C18AQ-250/212 -R 1u
50 x 30.0mm PF5C18AQ-050/300 -R 1u PF10C18AQ-050/300 -R 1u PF15C18AQ-050/300 -R 1u
100 x 30.0mm PF5C18AQ-100/300 -R 1u PF10C18AQ-100/300 -R 1u PF15C18AQ-100/300 -R 1u
150 x 30.0mm PF5C18AQ-150/300 -R 1u PF10C18AQ-150/300 -R 1u PF15C18AQ-150/300 -R 1u
250 x 30.0mm PF5C18AQ-250/300 -R 1u PF10C18AQ-250/300 -R 1u PF15C18AQ-250/300 -R 1u
50 x 50.0mm PF5C18AQ-050/500 -R 1u PF10C18AQ-050/500 -R 1u PF15C18AQ-050/500 -R 1u
250 x 50.0mm PF5C18AQ-250/500 -R 1u PF10C18AQ-250/500 -R 1u PF15C18AQ-250/500 -R 1u

Flash Columns 15µm RFID Qty 30µm RFID Qty

F0001 SC-15C18AQ-F0001 -R 25u --- -R ---
F0004 PF-15C18AQ-F0004 -R 4u PF-30C18AQ-F0004 -R 4u
F0012 PF-15C18AQ-F0012 -R 2u PF-30C18AQ-F0012 -R 2u
F0025 PF-15C18AQ-F0025 -R 1u PF-30C18AQ-F0025 -R 1u
F0040 PF-15C18AQ-F0040 -R 1u PF-30C18AQ-F0040 -R 1u
F0080 PF-15C18AQ-F0080 -R 1u PF-30C18AQ-F0080 -R 1u
F0120 PF-15C18AQ-F0120 -R 1u PF-30C18AQ-F0120 -R 1u
F0220 PF-15C18AQ-F0220 -R 1u PF-30C18AQ-F0220 -R 1u RFID Columns
F0330 PF-15C18AQ-F0330 -R 1u PF-30C18AQ-F0330 -R 1u add [-R] at the end of P/N
F0800 --- --- --- PF-30C18AQ-F0800 -R 1u
F1600 --- --- --- PF-30C18AQ-F1600 -R 1u

40 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

 puriFlash® Dry-Load
Solid Sample
Dry injection
High pressure dry-load

Allow to large sample amounts

Dry-load columns for solid deposits allow the injection of a raw sample insoluble (or soluble) in the mobile phase.
Compared to liquid injection, the solid deposit avoids the diffusion of raw sample in the purification column. It improves resolution, efficiency
and purity of the products collected.
The solid deposit can be made with silica, C18 or Celite. Unlike open cartridges, it does not require the use of a piston or specific adapters.
The max. pressure is 2x the standard solid deposit cartridges.
They are compatible with the use of Interchim® 15μm puriFlash® columns.
- Compatible with all purification systems

puriFlash® Dry-Load High-Pressure Dry-Load

Nature Type Format P/N Qty Nature Type Size P/N Qty
puriFlash® Dry-Load Empty F0004 PF-DLE-F0004 20u puriFlash® HP Dry-Load Empty 50 x 21.2mm OA0320 1u
F0012 PF-DLE-F0012 20u 75 x 21.2mm OA0330 1u
F0025 PF-DLE-F0025 20u 100 x 21.2mm 7A1870 1u
F0040 PF-DLE-F0040 20u 50 x 30mm OA0340 1u
F0060 PF-DLE-F0060 10u 75 x 30mm OA0350 1u
F0080 PF-DLE-F0080 5u 100 x 30mm 7A1880 1u
F0100 PF-DLE-F0100 5u puriFlash® Dry-Load - Tightening tool
F0120 PF-DLE-F0120 5u
Spanner wrench for 21.2mm ID 7A1590 1u
F0220 PF-DLE-F0220 5u
Spanner wrench for 30mm ID 7A1610 1u
F0330 PF-DLE-F0330 5u
Replacement frit
puriFlash® Dry-Load - Tightening tool JV0470 1u Replacement frit for 21.2mm ID 0A2100 1u
puriFlash Dry-Load
®
SILICA F0004 PF-DLSIHC08-F0004 20u Replacement frit for 30mm ID 0A2110 1u
HC 80% F0012 PF-DLSIHC08-F0012 20u
F0025 PF-DLSIHC08-F0025 20u
F0040 PF-DLSIHC08-F0040 20u
puriFlash® Dry-Load SILICA F0004 PF-DLSIHC05-F0004 20u
HC 50% F0012 PF-DLSIHC05-F0012 20u
F0025 PF-DLSIHC05-F0025 20u
F0040 PF-DLSIHC05-F0040 20u
puriFlash® Dry-Load CELITE F0004 PF-DLCET08-F0004 20u
80% F0012 PF-DLCET08-F0012 20u
F0025 PF-DLCET08-F0025 20u
F0040 PF-DLCET08-F0040 20u
puriFlash® Dry-Load C18 F0004 PF-DLIRC1808-F0004 5u
STD F0012 PF-DLIRC1808-F0012 5u
80% F0025 PF-DLIRC1808-F0025 5u
F0040 PF-DLIRC1808-F0040 5u
puriFlash® Dry-Load C18 F0004 PF-DLIRC1805-F0004 5u
STD F0012 PF-DLIRC1805-F0012 5u
50% F0025 PF-DLIRC1805-F0025 5u
F0040 PF-DLIRC1805-F0040 5u

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 Purification using PF-15C18HP-150/212
1. Analytical conditions
Solvents: A: Water
B: Acetonitrile (0.1% Formic acid)
HPLC Column: Waters Acquity BEH C18 250x4.6mm 1.7µm
Flow rate: 1mL/min
Injection mode: Liquid injection
Injection volume: 10μL
Concentration: 0.5mg/mL in Acetonitrile
Detection: UV 315nm
Elution conditions: 40% Water / Acetonitrile 60% (Isocratic mode)

Compound of interest

42 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

2. Prep conditions
Device: puriFlash® 4250 (or now puriFlash® 5.250)
Solvents: A: Water
B: Acetonitrile (0.1% Formic acid)
Column: PF15C18HP-150/212
Flow rate: 20mL/min
Injection mode: Liquid injection
Crude sample: 100mg
Detection: UV 315nm (black), Scan 200-600nm (orange)
Pressure: 7bar
Elution conditions:
t (min) A (%) B (%)
00:00 40 60
23:00 40 60
24:00 0 100
29:00 0 100

Small, but Limitless

250mL/min@up to 250bar

Purge Compound of interest

Method
GENIUS MODE

+
Sample
Queue

Live run

Reprocess

Admin

Settings

Log in

Log out

Off

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 Purification of mixture using PF-15SIHP-F0025
1. TLC method development

1
2
3

Mobile phase: Mobile phase: Mobile phase: Mobile phase:

90% Dichloromethane / 95% Dichloromethane / 50% Petroleum Ether / 50% Petroleum Ether /
Methanol 10% Methanol 5% Ethyl Acetate 50% Diethyl Ether 50%

O H O H O H

OH OH
O O
Mobile phase:
90% Dichloromethane /
O OH
OH O Methanol 10%

H O Compound of interest:
O H
compound 3
Close side products Compound of interest
Compound Rf CV
1 0.49 2.04
2. Choice of the column according to the ΔCV & crude sample mass 2 0.43 2.33
Crude sample: 47mg 3 0.34 2.94
Column: PF-15SIHP-F0025 4 0.22 4.54
Loading capacity: 0.25% ∆CV3-2=0.61
Loading Selection Guide for puriFlash® IR-50SI (Edition 2008-2017)

1600
Front Solvent

800

300
Optimal Rf range

RI: 0.35
Rf B

200
Rf A
Rt: 0.1
Column size (g)

120 Origin

CVA = 1/ RfA
80
CVB = 1/ RfB
CV = CVA CVB
CV
40
CV = (1 to 1,5)- Difficult

25 CV = (1,5 to 4)- Standard

CV = (4 to 10)- Easy
12

1 10 100 1 000 10 000 100 000 1 000 000

Sample size (mg)

Customer has chosen to use a PF-15SIHP-F0025 column to obtain a better separation (efficiency & purity) than with a IR-50SI-F0025 column.

44 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

3. Flash conditions
Device: puriFlash® XS 420Plus (or now puriFlash® XS 520Plus)
Solvents: A: Dichloromethane
B: Methanol
Column: PF-15SIHP-F0025
Flow rate: 15mL/min
Injection mode: Liquid injection
Crude sample: 47mg
Detection: UV 254nm (black), UV Scan 230-400nm (orange)
Mode: Automatic Gradient Optimization
Pressure: 1bar
Elution conditions:
CV A (%) B (%)
0 98 2
1 98 2
11 90 10
Small, but Mighty
13 90 10
300mL/min@up to 20bar

Purge
Compound 1 Compound 2 Compound of interest Compound 4

Method
GENIUS MODE

+
Sample
Queue

Live run

Reprocess

Admin

Settings

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 Purification of Peptides / Advantage of puriFlash® monolith columns
1. Analytical conditions
Solvents: A: Water + 0.1%TFA
B: Acetonitrile + 0.1% TFA
HPLC Column: puriFlash® BIO 100 C18-N 15µm 250x4.6mm
Flow rate: 1mL/min
Injection mode: Liquid injection
Injection volume: 10µL
Concentration: Mixture of Peptide standard (0.25mg/mL) and Protein standard (0.5mg/mL)
Detection: UV 215nm
Elution conditions:
t (min) A (%) B (%)
00:00 95 5
30:00 60 40

Compounds:
1. GLY-TYR (MW: 238 g.mol-1)
2. VAL-TYR-VAL (MW: 380g.mol-1)
1
3. Met-Enkephalin (MW: 574g.mol-1)
4. Angiotensin II (MW: 1 000g.mol-1)
2 5. Cytochrome C (MW: 11 749g.mol-1)

4
3
5

0 5 10 15 20 25 30 [min]

46 Interchim | Phone: +33 4 70 03 88 55 I Hotline: +33 4 70 03 73 01 I [email protected] I www.interchim.com I www.flash-chromatography.com

2. Flash conditions
Device: puriFlash® XS 420Plus
(or now puriFlash® XS 520Plus)
Solvents: A: Water + 1% TFA
B: Acetonitrile + 0.1%TFA
Column: puriFlash® Monolith C18 30µm F0025 (PM-30C18-F0025)
Flow rate: 15mL/min & 80mL/min
Injection mode: Liquid injection
Injection volume: 150µL
Crude sample: 9mg
Detection: UV 254nm (black), UV 215nm (orange)
Pressure: 2bar
Elution conditions:
t (min) A (%) B (%)
00:00 95 5
45:00 60 40
Small, but Mighty
300mL/min@up to 20bar

Purge
mAU 1.1 1.3 1.5 1.7 %
1.2 1.4 1.6
35.0 100
1
Method
GENIUS MODE 30.0
+ 25.0
2 80
Sample
Queue
20.0 60
15mL/min 15.0
5
3
Live run

10.0 40
4
5.0
Reprocess
20
0.0
-5.0 00
00:00 05:00 10:00 15:00 20:00 25:00 30:00 35:00 40:00 45:00

1.1
Admin
mAU %
30.0 1
Settings
25.0 80
5
20.0 60
2
15.0
80mL/min 3
10.0 40
Log in 4
5.0
20
Log out
0.0
-5.0 00
Off
00:00 05:00 10:00

Advion | Phone : +1-877-5-ADVION | [email protected] | www.advion.com 47