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Calibration of Refrigerated Centrifuge: D. Zheen J. Saber

1) The document provides steps for calibrating a centrifuge to prepare platelet-rich plasma (PRP) and platelet concentrates through centrifugation of whole blood. 2) Key steps include centrifuging whole blood at a calibrated speed and time to separate PRP from other blood components, then centrifuging the PRP at a higher speed to obtain a platelet concentrate. 3) The procedure involves counting platelets at different stages to calculate platelet yields and select optimal centrifuge settings that recover at least 75% of platelets from whole blood in the PRP or concentrate.

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Zheen Jawad
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71 views30 pages

Calibration of Refrigerated Centrifuge: D. Zheen J. Saber

1) The document provides steps for calibrating a centrifuge to prepare platelet-rich plasma (PRP) and platelet concentrates through centrifugation of whole blood. 2) Key steps include centrifuging whole blood at a calibrated speed and time to separate PRP from other blood components, then centrifuging the PRP at a higher speed to obtain a platelet concentrate. 3) The procedure involves counting platelets at different stages to calculate platelet yields and select optimal centrifuge settings that recover at least 75% of platelets from whole blood in the PRP or concentrate.

Uploaded by

Zheen Jawad
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Calibration of

Refrigerated
Centrifuge
By:
D. Zheen J. Saber

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Platelet count > 5.5 x 1010/unit in 75% of units.

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Materials:
1. Freshly collected whole blood.
2. Specimen of blood from the donor.
3. Metal clips and hand sealer of dielectric sealer
4. Clean instruments (scissors, hemostats, tubing
stripper)
5. Plasma extractor
6. Centrifuge
7. Worksheet for recording results.

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If one is to calculate RCF in g the following formula
should be used:

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Step1:Worksheet for preparation of PRP:
Centrifuge Time Donor Platelet Platelet count Platelet count RBC count Platelet
Speed In minutes number count on of whole blood of PRP of PRP yield %
EDTA tube

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Procedure of Calibrating centrifuge
For preparation of PRP :
Platelet count on anticoagulated specimen
1

• No. of platelet in whole blood= platelets/µL X1000 X ml of whole blood.


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• Prepare PRP at selected speed and time ( 2000 x g, 3 min + dec. time).
3

• Separate RBC and seal ( packed RBC+ 2 connected bags of empty & PRP).
4

• Strip the tubing several times so that they contain a representative sample of PRP.
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Continued:
Seal off a segment on the tail. Then perform platelet count in the sealed
6 segement of PRP.
• No. of platelet in PRP= platelets/µL X1000 X ml of PRP.
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• Calculate and record the % of yield= (no. of platelet in PRP X100)/no. of plt in WB.
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• Repeat 3-4 X (different speed, time and donor) compare yields.


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• Select the best. Record centrifuge ID, calibration settings, the date and name.
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Step2:Worksheet for preparation of
Platelets:
Calibration of hard spin

Centrifuge Speed Time Donor number Platelet count of PRP Platelet Platelet yield
In minutes count of %
platelet
concentrate

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Preparation of Platelets ( Hard spin)
Centrifuge PRP, according to….,(5000 x g for 7 min + dec. time)
1

• Remove clamp. Express plasma leaving 55 to 60 ml platelet volume.


2

• Seal tubing with long tube attached to platelet bag. Let platelets rest for 1 hour.
3

• Place the platelets on an agitator for at least 1 hour.


4

• Strip the tubing several times so that they contain a representative sample ofplatelet
5 concentrate.
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seal- segment-perform platelet count.

• Calculate and record the number of platelets in the concentrate.


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• Record no. of platelets in conc.= platelets/µL x1000x ml of platelets.


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• Calculate and record the percentage of yield.


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• Repeat with different donor PRP, speed and time, compare yields.
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• Select the best. Record centrifuge ID, calibration settings, the date and name.
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Notes:
• Quality control of centrifuge includes calibration of :
• speed and timer quarterly.
• temp check daily.
• Temp verification monthly.
• Calibrate each centrifuge individually.
When determining appropriate time and speed for centrifuge, consider
other products e.g:
Hematocrit and volume of packed RBCs.
Volume of plasma.

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Thank you
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Centrifuge

plasma
extractor

PRP Packed rbc

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Platelet-Rich Plasma method

Count the platelet of donor s specimine


Count whole blood platelet = plateletcount in µL x1000 x volume of the whole
blood unit

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centrifuge

Express with
plasma
expressor

PRP Packed rbc

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