Scribd
Upload
941 views9 pages

Autoclave: Definition and Usage

The document discusses the autoclave, a pressurized device used to sterilize equipment and materials by heating them above the boiling point of water under increased pressure. It provides a brief history of the autoclave from its development by Papin in the 17th century to its refinement by Pasteur and Chamberland in the late 19th century. The document also outlines how autoclaves work by using pressure to raise the boiling point of water, and describes their common uses in fields like microbiology, medicine, and dentistry.

Uploaded by

Rams Kb
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPT, PDF, TXT or read online on Scribd
941 views9 pages

Autoclave: Definition and Usage

The document discusses the autoclave, a pressurized device used to sterilize equipment and materials by heating them above the boiling point of water under increased pressure. It provides a brief history of the autoclave from its development by Papin in the 17th century to its refinement by Pasteur and Chamberland in the late 19th century. The document also outlines how autoclaves work by using pressure to raise the boiling point of water, and describes their common uses in fields like microbiology, medicine, and dentistry.

Uploaded by

Rams Kb
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPT, PDF, TXT or read online on Scribd
You are on page 1/ 9

Autoclave

By: Carolina Gallango-Brun Sana Khan Vedrana Sahovic

Definition
Autoclave is a pressurized device designed to heat aqueous solutions above their boiling point at normal atmospheric pressure to achieve sterilization
Fig. 1. Autoclave machine

Brief History
1450 B.C.E. Moses 460-377 B.C.E. Hippocrates Lazzaro Spallanzani Pasteur Denis Papin

Fig. 2. Papins steam cooker

20th Century
It was finalized by Charles Chamberland in 1987 Colleague of Pasteur Building up on Papins marmite From culinary to medicine
Fig. 3. Charles Chamberland 1851-1908

How Des it Works?


Liquid H2O cannot be heated above 100C in an open vessel At 100C boils H2O heated in a sealed vessel Pressure rises Boiling point of H2O is raised

The Process
Air removal Downward displacement Steam pulsing Vacuums pumps Superatmospheric Subatmospheric

Autoclave Usage
Microbiology Medicine Body piercing Veterinary science Dentistry Podiatry Metallurgy

Video Clip

References
http://en.wikipedia.org/wiki/Autoclave http://138.23.152.128/protocols/autoclave%20operation.pdf

You might also like