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lecture-8.bioinformatics-tools-for-lab

The document outlines various bioinformatics tools and resources for laboratory applications, including DNA reverse complement, translation of DNA, and measuring DNA and protein composition. It highlights tools like ExPasy for finding open reading frames and designing primers, as well as methods for identifying protein functions and targeting signals. Additionally, it references the signal hypothesis and post-translational modifications, providing links for further exploration of these topics.

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Zahra.
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9 views32 pages

lecture-8.bioinformatics-tools-for-lab

The document outlines various bioinformatics tools and resources for laboratory applications, including DNA reverse complement, translation of DNA, and measuring DNA and protein composition. It highlights tools like ExPasy for finding open reading frames and designing primers, as well as methods for identifying protein functions and targeting signals. Additionally, it references the signal hypothesis and post-translational modifications, providing links for further exploration of these topics.

Uploaded by

Zahra.
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Bioinformatics tools

for the
Laboratory
DNA Reverse Complement
• http://arep.med.harvard.edu/labgc/adnan/pr
ojects/Utilities/revcomp.html
Translation of DNA with ExPasy
Finding Open Reading Frames
nt

tide
stop codon

ea
o
ExPasy Tool
Measuring DNA Composition with DNAStats
Mapping restriction enzymes with NEB
Cutter
Primer designing with Primer3
Good pair
Measuring Protein Composition
Other protein characteristics
How to identify the putative function of a
protein sequence
Hydropathy plots
signal peptide
Each cell has
about 1
billion
proteins!

How do
proteins
know WHERE
to go in the
cell to do
their job?

How do
proteins
travel across
the
membranes
that
surround
most
“The signal hypothesis”: Proteins carry “tags” in primary
sequence which direct them to their final destination

Protein “zip-codes”

Gunter Blobel: 1999 Nobel prize in medicine

http://nobelprize.org/medicine/laureates/1999/press.html
Targeting signals are part of the primary amino acid sequence
S

S
SignalP---To remove signal peptide
ChloroP----To find Chloroplast signal
PTMs
15_22_glycosylated_ER.jpg
To find Post-translational modifications
If need more information, then
Go to
http://www.expasy.org/tools/

for
Practice

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