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LFA Presentation With Images

Lateral Flow Assays (LFAs) are rapid diagnostic tools used in various fields such as medical diagnostics, food safety, and environmental testing. They operate on capillary action and antibody-antigen interactions, providing quick results with advantages like portability and cost-effectiveness, though they may have lower sensitivity compared to lab tests. Future advancements include nanotechnology, smartphone integration, and AI-driven analysis to enhance their performance and accuracy.

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shraddhanandtiwari
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56 views12 pages

LFA Presentation With Images

Lateral Flow Assays (LFAs) are rapid diagnostic tools used in various fields such as medical diagnostics, food safety, and environmental testing. They operate on capillary action and antibody-antigen interactions, providing quick results with advantages like portability and cost-effectiveness, though they may have lower sensitivity compared to lab tests. Future advancements include nanotechnology, smartphone integration, and AI-driven analysis to enhance their performance and accuracy.

Uploaded by

shraddhanandtiwari
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Lateral Flow Assays (LFAs) - A

Deep Dive
Comprehensive Analysis
Presented by: Shantanu Tiwari
Affiliation: [Your Institution]
Introduction
Insert an image related to LFA introduction

• Lateral Flow Assays (LFAs) are rapid, point-of-


care diagnostic tools.
• Used in medical diagnostics, food safety, and
environmental testing.
• Examples: Pregnancy tests, COVID-19 antigen
tests, drug screening.
Principle of LFA
Insert an image explaining LFA principle

• LFAs work based on capillary action and


antibody-antigen interactions.
• A labeled antibody binds to the target analyte,
forming a complex.
• This complex travels along a membrane and
interacts at the test line.
• A visible signal is generated if the analyte is
present.
Key Components of an LFA Strip
Insert an image showing LFA components

• Sample Pad: Absorbs and distributes the


sample.
• Conjugate Pad: Contains labeled antibodies for
detection.
• Nitrocellulose Membrane: Houses test &
control lines.
• Absorbent Pad: Wicks excess fluid away to
maintain flow.
Types of LFAs
Insert an image illustrating different types of LFAs

• Sandwich Assay: Used for large analytes like


proteins and viruses.
• Competitive Assay: Suitable for small
molecules like drugs and hormones.
• Multiplex LFAs: Capable of detecting multiple
analytes in a single test.
How LFAs Work - Step-by-Step
Insert an image describing the LFA process

• Sample is applied to the strip.


• The liquid migrates via capillary action.
• Labeled antibodies bind to the target analyte.
• The complex reaches the test line, generating
a signal.
• Control line ensures test validity.
Applications of LFAs
Insert an image showcasing LFA applications

• Medical Diagnostics: Pregnancy tests, COVID-


19, HIV, cardiac biomarkers.
• Food Safety: Detection of allergens, toxins,
and contaminants.
• Drug Testing: Rapid detection of illicit drug
metabolites.
• Veterinary Medicine: Detection of animal
diseases.
Advantages & Limitations
Insert an image showing advantages & limitations

• ✅ Rapid results within minutes.


• ✅ Cost-effective and easy to use.
• ✅ Portable for point-of-care testing.
• ❌ Lower sensitivity than lab-based tests.
• ❌ Potential for false positives/negatives.
• ❌ Limited quantitative measurement.
Future Prospects in LFA Technology
Insert an image representing future LFA advancements

• Nanotechnology-based enhancements (gold


nanoparticles, quantum dots).
• Smartphone-integrated readers for improved
accuracy.
• CRISPR-based LFAs for high-sensitivity genetic
testing.
• AI-driven analysis for better interpretation.
Conclusion
Insert an image summarizing the topic

• LFAs play a crucial role in rapid diagnostics.


• They are widely used in healthcare, food
safety, and drug testing.
• Future advancements will improve sensitivity
and accuracy.
• Their affordability and ease of use will ensure
continued adoption.
References
Insert an image related to references

• WHO reports on lateral flow diagnostics.


• Research papers from NIH and scientific
journals.
• Recent advancements in LFA technology.
Q&A
Insert a Q&A related image

• Open for questions and discussions.

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