Alliance Separations

Module

2695 培训

©2007 Waters Corporation

 2695 Features

• Solvent Management vs. Pump • Detector flexibility

• – UV (2)
Sample Management vs. Autosampler
– RI (1)
• Integrated Solvent & Sample Manager

Base 2690 2690XE

▪ Quaternary GPV
▪ 120 sample vial capacity • Base 2690 plus:
— Std. 2ml vials with optional LVI
▪ Independent needle wash
• Inline degasser
▪ Independent plunger seal wash • Column heater or Column heater/cooler
▪ Helium Sparge - solvent conditioning*
• Sample Heater / chiller
▪ IEEE & RS232 controllable
▪ Floppy disk drive
▪ Solvent bottle tray*

©2007 Waters Corporation 2

 Options

¡ 2695 ▪ Field Installable Column

Switching Valves
— Syringes – µL — 2 Position
Regeneration
o 250µL - standard — 3 Position Selection
o 25µL, 2500µL — 6 Position Selection
— Sample Loops – µL
o 100µL - standard
o Variable up to 2ML
— Weight = ~130lbs
— Volume = <650µL
— # Samples - 120

▪ Column Heater
— Without Valve (default)
▪ Column Heater/Cooler

©2007 Waters Corporation 3

 Alliance Option Kits

▪ All options are field installable:

— Plunger Seal Wash (WAT270872)

— PerformancePLUS Degasser Tray (WAT270873)

— Sample Heater Cooler (WAT270802)

— Column Heater (186001863)

— Column Heater / Cooler (186001791)

o 3Column Switching valves
– Three-Column Select valve kit 205000162
– 2 Column regeneration valve kit 205000163
– Six-Column Select Valve kit 205000164

©2007 Waters Corporation 4

 Component Layout

Left Side

Right Side

Rear Panel

©2007 Waters Corporation 5

Alliance Solvent Conditioning
®
— Tubing Flow Path
— Piston Drive
— Start Up
— Priming
— GPV
— Degasser
Assembly
o Old Style
o Current Style

©2007 Waters Corporation

Alliance Start Up Flow Chart
2695

©2007 Waters Corporation 7

Priming the Solvent Manager

• Properly degas your

solvents before
introduction

• Use solvent with the

lowest viscosity for initial
priming

©2007 Waters Corporation 8

 Principal Primes

Solvent Manager
▪ Dry Prime:
— Opens the fluidic path to replace air with solvent, then performs a
prime.
▪ Wet Prime:
— Replaces solvent in the path from the reservoirs to V1 & V2 and then to
waste (V3). Used to change solvents in the system.
▪ Seal-Wash Prime:
— Lubricates each plunger & flushes away solvent or dried salts. Extends
the life the plunger seals.

▪ Needle-Wash Prime: Sample Manager

— Flushes the needle in the Sample Management system

preventing carryover or contamination. Extends the life of the
injector seals.

©2007 Waters Corporation 9

 Dry & Wet Prime

▪ Use “Dry” Prime when the system is dry.

▪ Use the “Wet” Prime to change solvents or if instrument has

been idle for a long period of time.
— Run slow flow following to replace with degassed solvent.

▪ Complete Remaining Prime Functions.

©2007 Waters Corporation 10

 Solvent Management –
Serial Flow Path

Prime/Vent
Valve

D A
Inlet Inlet
Checkvalv Checkvalv
e e

Front
C B

©2007 Waters Corporation 11

 Piston Drive Tray

Vent Piston Drive

Filters Motors
Plunger Driver
Board
Accumulator Piston
Drive

Checkvalv
e Primary Piston
Drive
Prime / Vent
Valve Top = Acc
Transducer Bot = Pri
s
Plunger Wash
Pump Checkvalv
e

©2007 Waters Corporation 12

Piston Drive Tray

• Pay attention to correct

Drive Motors

• Be sure to lock CPU cable

©2007 Waters Corporation 13

 Piston Drives
• Changes include:
– new style ball screw, which drives the internal
mechanics for the piston.
– changed lubricant from the original oil to
grease.
– larger, metal breather vent to aid in improved air
movement in the piston drive.

Plunger Drive Assemblies

are not interchangeable
and can not be mixed
Transducers

©2007 Waters Corporation 14

External Tubing

2795
Only

©2007 Waters Corporation 15

 Solvent Conditioning
▪ Modified GPV assembly
— Internal accumulators (diaphragms)
— High Flow tubing - yellow (.040”)
— Standard tubing - black (.030”)
— Low Flow tubing - green (.020”)

There is a 5msec pause

before voltage is applied to the
next valve so that no more
than one valve is open at any
time.

©2007 Waters Corporation 16

 Alliance Solvent Conditioning
• Sparge Assembly
Tray
– Industry standard
1/8” fittings

PerformancePlus Degasser Chamber

Recommended Max 5mls/min

Flow rate

Internal Volume 500uls

Internal Tubing 0.045” • Current Style

Diameter
Degasser
Flush Volume (4x) 2mls
Tray
– 4
channel only
©2007 Waters Corporation 17
 Current Degasser

▪ Vacuum Pump runs at High Speed (400 RPM) until a

vacuum level of 0.9psia is reached.
— Once 0.9 is reached within 10 minutes, then the speed drops
down to 60 RPM

400 RPM

0.9psia

60 RPM
6.5psia
Vacuum motor
RPM Vacuum level
PSIA

©2007 Waters Corporation 18

2695 Status Layout

Only the options

installed will be
displayed.

©2007 Waters Corporation 19

 Direct Functions

©2007 Waters Corporation 20

 Alliance®
Programming
and Operating

©2007 Waters Corporation

 File Extensions

*.MTH (export) vs. *.PRN (print)

-------------------------------------- ---------- ---------------- ------------
」 4 Waters 690 Method 「 」 」 User 」 User 」 coke · S E P A R A T IO N M E T H O D : C O K E
S E R IA L N U M B E R : J9 8SM 4255R
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011099 」 「 AÈ AÈ H ig h p re s s u r e a la r m :
B u b b le d e te c t a la r m :
4 0 0 0 p s i A le rt u s e r
A le rt u s e r
@@ 「 「 F lo w R a m p :
S o lv e n t n a m e s :
1 .0 0 m in (1 0 .0
m L /m in /m in )
「 ¿€C~
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Bp B「 ?
¿€ D e g a s s e r fa il a la rm :
P u m p s e a lw a s h p e rio d :
A le rt u s e r
1 .0 0
€ P re fe rre d s tro k e v o lu m e : A u to m a tic

¿€ 丁 G ra d ie n t: ( m in ) %D C u rv e
(m L /m in ) % A %B %C
@ 1 . IN IT 3 .0 0 0 6 0 .0 4 0 .0 0 .0
(2 ) S A M P L E 0 .0
¿€ 」 ? S a m p le te m p e ra tu re : 40C
€ S a m p le te m p a la rm :
S y rin g e d ra w (ra te ):
A l e rt u s e r + / - 3 C
N o r m a l (2 .5 0 u L /s e c )
¿€ (d e p th ): 1 m m fro m b o tto m o f v ia l

C~ (3 ) A U T O S A M P L E R
P re -c o lu m n v o lu m e : 1 5 0 .0
¿€ 「 uP L
o s t-ru n d e la y : 0 .5 0 m in
C o m p re s s io n c h e c k a la r m : A le rt u s e r
C~ (4 ) C O L U M N
¿€ 「 「 C o lu m n in fo r m a tio n :
0 11 0 9 9
?€ (5 ) I/O
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¿€ 「 丁
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@ N o d e te c to rs e n a b le d
¿€ 「 」 O V E R V IE W (m in ) A c tio n
?€ 1 . IN IT 3 .0 0 0 m L /m in 6 0 .0 % A 4 0 .0 % B 0 .0 % C 0 .0 % D c 6

¿€ 「
C~
©2007 Waters Corporation 22
2695 Programming

©2007 Waters Corporation

 Screen Hierarchy

Dual Mode
Concept
Main menu screen Status screens
• Used to access main • Used for direct control
software

©2007 Waters Corporation 24

 Develop Methods

©2007 Waters Corporation

25

Separation Method
Sample Set
Template Sample
Set
Creating New Method

©2007 Waters Corporation 26

 Method
- Page 1

• A 0psi setting for

Min pressure will
disable low pressure
alarm.

• Flow Ramp also

affects the
decrease in flow.

16 characters in
v Rev 2.02 +
sample name
©2007 Waters Corporation 27
 Method
- Page 2

Operating Range
Default
4° - 40 °C minimum
Degradation Point 0.080”
29 °c
needle to
vial

Slow = 1mL/sec
Normal =
2.5mL/sec Fast =
5.0mL/sec ©2007 Waters Corporation 28
 Method
- Pages 3/4/5

“Normal” = 13 seconds

2690 System
volume is
<650mL

©2007 Waters Corporation 29

 Method
- Page 6

©2007 Waters Corporation 30

Sample Set Views

©2007 Waters Corporation 31

 AutoAdd

©2007 Waters Corporation 32

 Configuration

System
Control

©2007 Waters Corporation 33

 Detectors

©2007 Waters Corporation 34

 Installed Options

2690

Absolute or
Relative Pressure
Sensor – different
scale

• 2695 shows a filled-in

box instead of a check
mark. 2695
• All Degassers in the
2695 are
PerformancePlus
• Absolute Sensor = 0 to
+7
– closer to 0, the better
• Relative Sensor = 0 to -
15 35
©2007 Waters Corporation
– more negative, the better
 Configure Reports & Compression Check

2690

©2007 Waters Corporation 36

Auto-Shutdown & Error Log

©2007 Waters Corporation 37

 – Start-Up
Hands On Exercise

▪ Connect all power and fluidic

connections
▪ Perform: as applicable
— Dry prime
– Wet prime
— Instrument purge
— Seal Pack Adjustment
— Compression test
— Static leak test
– Try only once

©2007 Waters Corporation 38

Alliance “e” Series Enhancements

▪ New front bezel – no floppy drive

▪ New keypad
▪ New CPU
▪ Silver label “e2X9X”
▪ RS232 connector – only 1
▪ New Ethernet connector
▪ System Prep Functionality

***New Alliance spares should be only used on Alliance

“e”Series instruments***

©2007 Waters Corporation 39

New Keypad, CPU Rear Panel and Label

©2007 Waters Corporation 40

New Alliance “e” Series CPU

— All Alliances
o 2695,2695D, 2795, 2796

— No boot proms

— Backward compatible

— Requires Firmware v3.00

or newer

— 1 RS232 connector
— Ethernet connector

©2007 Waters Corporation 41

V3.00 Firmware differences

▪ System PREP – same as 2795

— One touch Purge/Wet prime function in Menu/Status
— This is recommended for systems that are already wet and need a
quick refreshing of solvents.

▪ Some Defect Fixes

— Stopped “Changing composition when Menu/Status to Diag”
— Corrected GPV Test Method
— Degasser is On at power up
— High pressure alarm is disabled during Purge
— Floppy OK is no longer displayed during S/U
— Stroke volume no longer changes temporarily on remote
methods
— “Press the ?” appears when there is an Alarm Message
— 2795 only- No Flow Shutdown method, similar to the 2796
— 2795 – SLT changed from 20% to 50% pump stroke and message of
“Passed” is replaced by “Test Complete”

©2007 Waters Corporation 42

2 Alliance Upgrade Paths

Ethernet Conversion Program Kits

▪ New CPU - ***old CPUs must be returned to Milford***
▪ Firmware v3.00
▪ Ethernet Switch Communications Kit
▪ Label

¡ 176001760 2695 Ethernet Conversion Program

¡ 176001761 2695D Ethernet Conversion Program
¡ 176001762 2795 Ethernet Conversion Program
¡ 176001763 2796 Ethernet Conversion Program

Note: This is NOT a free upgrade solution. Entitled

customers who wish to upgrade will receive the kit at a
discounted price.

Firmware
¡ Update
667003701 KitsFIRMWARE UPDATE v3.00
KIT, 2695
¡ 667003702 KIT, 2695D FIRMWARE UPDATE v3.00
¡ 667003703 KIT, 2795 FIRMWARE UPDATE v3.00
¡ 667003704 KIT, 2796 FIRMWARE UPDATE v3.00

©2007 Waters Corporation 43

Ethernet Kits

700004123 ETHERNET
SWITCH COMMUNICATION KIT
▪ Install an 8-port Ethernet switch to the rear panel of
Alliance Separations Modules or 1500 Series HPLC Pumps
using the supplied mounting bracket.

¡ 725000455 SWITCH, ETHERNET, 10/100, 8 PORT

¡ 441000371 ETHERNET PATCH CORD, SHIELDED, 25' L
¡ 441000562 ETHERNET PATCH CORD, SHIELDED, 2' L
¡ 400003086 BRKT, MTG, ETHERNET SWITCH
¡ 410000419 SCREW, M4 X 10, SEMS
¡ 715001744 Installing Ethernet Switch and Mounting Bracket

©2007 Waters Corporation 44

eProducts Service CD

¡ PN 667003687
— Firmwares (.sfts):
o 2695/D, 2795, 2796, 2414,TCMII, PCMII, 15xx,1500CH, 2475
— Autoloader utility used to facilitate to update firmware.
— Alliance Log utility used to capture trace, error, and lifetime
history.
— Release notes for individual components
— An installer to deliver all these utilities to the host PC

▪ Floppy files will also be available from the Portal and

Service Notes

****SERVICE ONLY****

©2007 Waters Corporation 45

Downloading Trace Logs

Via Ethernet

▪ Requires Alliance Log Tool from eProducts Service CD

— If Empower is installed, loads to Empower/Instrument/Utility
— If MassLynx is installed or no data acquisition software installed, loads to
Program Files/Waters Instruments/Utility
▪ eAlliance Configuration set to Empower and Auto/Ethernet
▪ Crossover ethernet cable AND/OR go thru Ethernet Switch box
▪ Computer IP address of 192.168.0.1
▪ Floppy is not connected

Via Floppy

▪ Working floppy drive

▪ Floppy disk

©2007 Waters Corporation 46

Alliance “e” Series Log Manager

Empower
Instrument
Utility
Alliance
LogToo
l

Password: 1147
▪ Needed to retrieve zip
files

©2007 Waters Corporation 47

eProducts Supported Instrument Configurations

We only support homogenous configurations (all IEEE or all

Ethernet) with the exception of the 2707, 2489, 2998, 2424
and 2475 as these components do not support IEEE at all
and have been already introduced into heterogenous
instrument configurations.

Notes: 1. Do not connect both Ethernet and IEEE cables to an

instrument. Empower Node and TCP/IP proxy will cause
instrument failures upon instrument setup.

2. Set the serial number on all modules after the

firmware has been updated. This will allow the module to
be properly recognized under Empower FR5
Ethernet control.

©2007 Waters Corporation 48

2695 Injection Flow Path
Injection Sequence

©2007 Waters Corporation

Sample Manager Main Components

Clear
Tubing

Red Line
- solvent out Blue Line - solvent in

Seal Pack

©2007 Waters Corporation 50

Purge Cycle - Part I

Needle in
stream
Position

©2007 Waters Corporation 51

Purge Cycle - Part II

©2007 Waters Corporation 52

Purge Cycle Part III (Compression Check)

Needle
in lower seal

©2007 Waters Corporation 53

Injection Cycle

V2 & V3 Open
to equilibrate
the Sample
Loop

Needle in
Seal
Position

©2007 Waters Corporation 54

Sample Withdraw - Part I

Needle in
Vial

©2007 Waters Corporation 55

Sample Withdraw - Part II

Needle in
Vial

©2007 Waters Corporation 56

Sample Injection

©2007 Waters Corporation 57

Alliance Solvent Manager
®

▪ Solvent Pump Tray

©2007 Waters Corporation

 Head Assembly – Cross Section

▪ Online mixing is
accomplished in the
head assemblies

▪ Solvent enters the head

through the ‘J’ Tube
— First in = First out

▪ 140ul plunger cavity

volume

©2007 Waters Corporation 59

 Plunger Travel
A. Maximum Plunger Travel
▪ 14,550 Motor Steps

B. Plunger Travel Buffer Zone

A
▪ 300 Motor Steps
C
B C. Plunger Home Position
▪ 14,250 Motor Steps
o ~101 steps/µL
▪ ~141µL Delivery
Volume

▪ Maximum Programmable Stroke

Volume:
▪ 130µL – 13,135 Motor Steps
o ~101 steps/µL

©2007 Waters Corporation 60

Flow ©2007 Waters Corporation 61

Path

Head
Assy
.
 Stroke Volume

▪ Stroke volume is pre-set in the

“Default Separation Method”
to 130uL.

▪ Selection of an alternative stroke

volume is recommended,
depending upon flow rate – refer
to the table below:

Flow range (ml / min) Stroke volume (µL)

0.010 – 0.530 25
0.531 – 1.230 50
1.231 – 3.030 100
3.031 – 10.000 130

©2007 Waters Corporation 62

 Bubble Detection

▪ Activates:
— 650psi or higher

▪ Compression / Decompression Ratio:

— 1 - 1.4 = Normal
— 1.4 -1.8 = Fair
— > 1.8 = Bubble Detection Occurs

▪ Bubble Recovery Mode

— Pre v2.02 only

©2007 Waters Corporation 63

 Operating Pressures

Flowrate setting Maximum Operating Pressure

0.001mL – 2.999mL 5000psi
3.0mL – 4.999mL (linear decline) 5000psi – 4000psi
5.0mL – 10mL 2000psi

Pressure 5
(kpsi)
4

0
1 2 3 4 5 6 7 8 9 10
Flowrate (mLs/min)

©2007 Waters Corporation 64

▪ Alliance Sample Managers
®

¡ Components

©2007 Waters Corporation

 2695 Sample Management
▪ 5 carousels
— 24 vials each (120 total)
— Color coded and alphabetized (A-B-C-D-E)
▪ Rebuildable seal pack
▪ Std. Syringe 250µl
— (25 & 2500 optional)
▪ Std. 100µl sample loop
o Configurable options
▪ Vial detection
— Fiber optics
— Flashing red LED

©2007 Waters Corporation 66

 2695 Sample Manager

Carousel Drive Transducer Needlewash Pump

Syringe Drive
Col Htr Bd

V2

V3
Injector
Assy.

V4

V1

Carrier Drive
Bar Code Reader

©2007 Waters Corporation 67

 Injector Assembly

▪ Injector Sensor
Adjustment
5/16”
— Starting point of
5/16” gap

— One rotation =
Aprox. 20 motor
steps

— Loosen bottom
Stream screw and adjust
position top

Seal
Count
Sensor

Rebuildable Seal Pack

©2007 Waters Corporation 68

 New Style Injector

▪ Enhanced
version injector
assembly
▪ Uses existing Seal Pack
Rebuild
▪ Backwards Compatible

©2007 Waters Corporation 69

Syringe
Drive
Assembly ▪ Common Syringe
Drive Assembly
used in the 2695
and 717+
— From 25µL -
2500µL

Travel Limit
Switch

Syringe
Seal
Syringe
Tip Syringe
Plunger

©2007 Waters Corporation 70

 Carousel / Carrier Drive Assembly

▪ Carousel / Carrier Drive Assembly

operate separately to rotate the
proper carousel and/or vial under
the injector.

Additional Details
to follow in
Troubleshooting
section

©2007 Waters Corporation 71

 Transducer

▪ The system pressure transducer measures the operating

pressure. A software algorithm compares the primary head
pressure and the system pressure readings and regulates
the pre-compression step to balance the pressures,
providing a smooth, ripple-free flow.

Current

Obsolete

©2007 Waters Corporation 72

 2695 Needle wash

▪ The needle wash system consists of a 24 VDC diaphragm

pump and a 3-way solenoid valve (V4) on the input side of
the pump that opens to admit wash fluid and closes to
admit air.

©2007 Waters Corporation 73

 Solvent Select Valves

Three Six Position Two Position

Position Select Regeneration
Select Valve Valve Valve

• Example of plumbing • Used on 2nd Generation • Regenerant used to keep

– Column Alliance Column Heater. off-line column
equilibrated
– Waste for HT • New Column Heater works
flushing with 2695, 2695D, 2795 • Regenerant pump
– Restrictor for and 2796. – 510, 515, 600, etc
MS Flow
Injections
©2007 Waters Corporation 74
2695 HPMV – V1 & V2

HPMV Rebuild Kit

WAT045424
©2007 Waters Corporation 75
 Low Pressure Valve

▪ A new, improved V3 solenoid valve was released in late

2000. The flow path through the new valve is less
susceptible to particulate matter, which can be trapped
between the diaphragm and seat.

• Watch for peek

ferrules when
disassembling

©2007 Waters Corporation 76

Sample Heater Cooler

¡ 4º – 40ºc

▪ Temperature
degradation
point = 29º

▪ Rear Panel with

Sample Heater
Cooler
Removed
©2007 Waters Corporation 77
 Hands on Exercise - Main Components

▪ Remove, Inspect, Reinstall all components

— Remove Solvent Management system
o Remove Solvent Management Tray
o Remove Solvent Conditioning Tray

— Remove 2695 Sample Mgmt system

o Review C/C Drive Assy., Injector, HPMV, V3, V4, Needle Wash Pump, Syringe
Drive Assy., Transducer, Front Panel
o Remove Sample Heater/Cooler

— Remove and review PCB’S and Power Supply Assy.

Use Caution when handling printed circuit boards

©2007 Waters Corporation 78

Electronics

©2007 Waters Corporation

 Electronics

CPU PCB
Front Panel PCB

Power Supply
• 600 watt only
Fluidics Driver Board

▪ Plunger Driver PCB

▪ Degasser Control PCB
▪ Sample Heater Cooler
▪ Column Heater
— Column Heater Interconnect Board

©2007 Waters Corporation 80

 Front Panel Board

•LCD lamp voltage is over 600

Display
volts at power-up then drops MUST be
to an operating voltage of 300
volts. grounded
• Generated from +5VDC

Keypad
Membrane LCD

LCD Controller / Keypad

Encoder ©2007 Waters Corporation 81
 CPU Address
Switche
-sold style

Serial
Option
Ports
• 4 Total
Firmware
-old style

Speaker
Floppy
Battery Drive
Processo
r

Serial Cable
Transducer Plunger Drive to Plunger
s Motors Tray
©2007 Waters Corporation 82
 CPU

▪ Diagnostic
▪ LED’sPROMS: WAT271250
Boot

©2007 Waters Corporation 83

 600W Power Supply

Power Supply is not serviceable.

Replace if faulty.

©2007 Waters Corporation 84

 Fluidics Driver Board

Sensitivity
Adjustmen
t

Fiber Optic Control Sensor

v Green LED
v P ower
v Yellow
LED
v Sig
nal
LEDs light, only when
looking for a vial
©2007 Waters Corporation 85
Plunger Driver Board

▪ In the event
of a Solvent
Delivery Error,
check F1 (7A
Fuse).
WAT290188

©2007 Waters Corporation 86

 Degasser Control Board
The Degasser
©2007 Waters Corporation
is always:
• On 87

• Off
Sample Heater / Cooler
Current Style
Temperatur
e Probe

2” Internal Fan
for circulation

2 – 4”
Externa
l Fans

Heater
Chiller
Engine
See Service
Side Mounted Note:
Control Bd.
for easier 2690 Heat er
access Chi l l er
Updat e
©2007 Waters Corporation 88
 Original Alliance Column Heater

▪ 4 column capacity
▪ Preheat tube
—  1ml not necessary
– Coil red line in column heater
—  Install preheat tube and coil red
line
▪ See Service Note:
— Repair of Alliance Column Heaters
▪ Externally connected
— Interconnect cable
– Column heater interconnect board

Do not connect or remove while live

Damage to COMM port on CPU will occur
©2007 Waters Corporation 89
Old, New, and Newest

©2007 Waters Corporation 90

 Column Heater Option Board

• F1= 24v
– 5a replaceable
WAT290197

• F3= 5v
– 1.5a soldered

• F2= 5v
– 1.5a soldered

Do not connect or remove while live

Damage to COMM port on CPU will occur

©2007 Waters Corporation 91

2nd Gen Col Htr & Column
Heater/Cooler
• 2 columns up to 300mm w/guard columns
• A grommet for mounting a calibrated thermister
for qualification
• A smaller door hinge allows tubing to be routed out
the right side
• No pre-heater tubing
• The switching valve has been moved forward for
easier access
– Alliance Column Heater/Cooler
– From ambient –15°C up to 65° C
– Internal power supply to drive the heater cooler engine
– Separate power switch on right side
– Alliance Column Heater
– Ambient to 65° C
– Similar heater coil for convection heating
• Both models:
– Interface 2695, 2695D and 2795, (minimum Rev.
2.02)
– Common PCB, includes valve driver circuit
©2007 Waters Corporation 92
 Alliance Column Heaters

Alliance 2nd Generation

Alliance Column Heater/Cooler Column Heater
• Use of peltier chips • convection heating

Oven Compartment Oven Compartment

JTAG
JTAG

Over Temperature Over Temperature

Switch Switch
Valve
Valve Select
Select
Thermistor Switch Thermistor
Switch

Column Heater/Cooler Column

Internal Internal
PCB Heater/Coole Fan
Fan r PCB
Connects Connects
To 2x9x To 2x9x
Door Switch Door Switch

Thermoelectric
External Heater/Cooler
Fan

Heater

24 Volt
Power
Supply
A jumper is added to J7, (the power
connector) which configures the
PCB for a heater only
Power Entry
Module
AC Position Sensors
Position Sensors
Power FRONT
FRONT REAR PANEL VALVE
Cord REAR PANEL VALVE PANEL
PANEL

Power
Switch

©2007 Waters Corporation 93

2695 Diagnostics

©2007 Waters Corporation

 Diagnostics

2690 2695

©2007 Waters Corporation 95

Run Samples

Diagnostics

©2007 Waters Corporation 96

Compression Check & Adjust Seals

©2007 Waters Corporation 97

 Other Diagnostics

©2007 Waters Corporation 98

 Other Tests

©2007 Waters Corporation 99

 Other Tests - cont.

10
©2007 Waters Corporation 0
Other Tests - cont.

10
©2007 Waters Corporation
1
 Service Utilities

Password = 1147
0.5V +/- 0.2V

See Service
Note on
Transducer
Seal Wash s
Pump
Activation

• Default at start-up
• Not necessary with
current vacuum
sensor

Contact Global Service Support before making

adjustments ©2007 Waters Corporation
10
2
Service Utilities - Custom Offsets

▪ Software adjustment for

Carousel Ram
positioning
▪ Vial
— (+) = needle up
— (-) = needle down
▪ Z-Axis
— (+) = dog ram down
— (-) = dog ram up

Current Sample
Compartment Material

10
©2007 Waters Corporation
3
Carousel/Carrier Drive Height Adjustment

Fig. 1

Z-Axis
Ram
Height
Gauge

P/N 700001022
Fig. 2

Bracket-Securing Screw

Adjustment Screw

10
©2007 Waters Corporation
4
Carousel Rotation Adjustment

• To correct issues with vial septa not pierced in the

center
• To rotate carousel CCW, turn sensor CW.
• To rotate carousel CW, turn sensor CCW. 10
©2007 Waters Corporation
5
Carrier Rotation Adjustment

• Used to adjust positioning

of carousel beneath Ram

• Turn sensor CW to rotate

carrier CW

• Turn sensor CCW to

rotate carrier CCW

10
©2007 Waters Corporation
6
Hands on Exercise - Programming

Familiarize yourself with the

screens
▪ Direct Functions

— Create Methods
o Sample and Instrument
o Make an injection

▪ Service Diagnostics

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©2007 Waters Corporation
7
 Alliance Service
—PM
® Procedures
Procedures
oPM Test
—Calibration
Protocol

©2007 Waters Corporation

 Plunger Drive Assembly

Follow steps closely

Indicates
Direction of
Solvent
Flow

1/8” – ¼”
Turn Only

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©2007 Waters Corporation
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 Check Valve Summary
New Desc. Old Desc. Material P/N (2) PM Kit Application Used With

PerformancePLU PerformancePLU Ruby 700000254 Y Standard Check 2695, 2795,

S R/S CV Cart. S Sapphire CV Sapphire Valve 515, 600,
(2) Cart. (2) 1/8 1525

PerformancePLU PerformancePLU Ceramic 700002399 N Option – sticking 2695, 2795,

S C/C CV Cart (2) S Ceramic CV issues, wide range 515, 600,
Cart. (2) of flow rates 1525

2X95 R/S CV 2690 Inlet CV (2) Ruby WAT270941 N Option – Lower 2695, 2795
Cart. (2) /Sapphire Internal Volume
1/16”

2X95 C/C CV Separations Ceramic/Cer 700002761 N Option – sticking 2695, 2795

Cart. (2) Module Ceramic amic. 1/16” issues, lower
CV Cart. (2) internal volume

Discontinued Separations Ceramic 700002333 N Discontinued Replaced by

2X95 Ceramic Module Ceramic 1.75 700002761
CV CV Cart. (2)

Ceramic Ball & Seat = Cartridge

will have ‘C’ stamped on it.

1/8” = PEEK Gaskets

1/16” = Black Gaskets
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9
Rebuilding the Head and Seal Wash Chamber

• Seal Wash Face

Seals
– Wash
• Seal Not Replaced
Plunger Seal
(WAT270160)
• 2- Seal Wash Tube seals
(WAT270669)
(Not in 2695 PM
kit)
WAT271017

• Face Seals (WAT270163)

– 4 = 2 in each head
• Plunger Seal
(WAT270789)
– 2 = 1 in each head
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©2007 Waters Corporation
1
 Service Procedures

▪ Head seal
replacement Orange, Floraloy G
— Plungers seals
material Standard
— Face seals
seal
Good for most
• Seal wash chamber seal applications
– Plunger seals
replacement Mix of organic / H2O
– Face seals Black, GFP material
– Tube seals Optional seal
Good for 100%
• Seal pack rebuild
organic Clear, UHMWPE
– HP seals / misc.
material
components
Optional seal
Good for 100%
organic
700004073- PM SOLVENT
MANAGER
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©2007 Waters Corporation
2
 2695
Sample
Manager

©2007 Waters Corporation

 2695 Performance Maintenance Kit

P/N# WAT270944
▪ Solvent Diffuser WAT007272 1
▪ Oriented plunger WAT270488 2
▪ Check valve cartridges 289000144 2
▪ Face seals WAT270163 4
▪ Plunger seals WAT270789 2
▪ Seal pack rebuild kit w/needle WAT271019 1
▪ Seal wash tube seal WAT270668 4
▪ Seal wash plunger seals WAT270160 2
▪ Syringe WAT073109 1
▪ Battery WAT080443 1
▪ Solvent filters WAT025531 4
▪ Filter insert WAT088084 1

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©2007 Waters Corporation
4
 Performance
Maintenance Visit
Features
▪ During the Performance Maintenance Visit the FSE will
check whether the current version of firmware is installed
— If the instrument is not at current revision, the FSE consults
with customer to advise about the features/benefits of the
latest version
— The FSE reviews the error log (Alliance) or trace log (ACQUITY)
for potential issues
o During a corrective repair event the FSE can review the logs to help
troubleshoot the issue

Dust Kit 700002665

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5
 Specialty Tools

Seal Extraction 2695 Seal Insertion

Tool Tool
WAT039803 WAT270969

Standard Seal
High Insertion Tool
Pressure
Seal
and Seal Guide
Insertion Tool WAT024923

& Bullet
WAT045427

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©2007 Waters Corporation
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Old Seal Pack Rebuild

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©2007 Waters Corporation
7
Rebuilding The Seal Pack
1.Separate the upper seal wash body, the seal body, and the lower seal wash body.
Rebuild Upper Seal Wash Body

1.Remove the frit retainer from the upper seal wash body and remove the needle wash filter and TFE washer
from the frit retainer.

2.Using the plastic end of the seal removal tool (WAT039802) remove the upper spacer from the upper seal
wash body.

3.Wet the new upper spacer (WAT031957) thoroughly with methanol or compatible solvent and press into
place in the upper seal wash body using a flat end.

4.Insert a new TFE washer (WAT077321) and needle wash filter (WAT072324) into the frit retainer and
screw the assembly into the upper seal wash body finger tight.

Rebuild Seal Body (skip this step if replacing seal body)

5.Using the seal removal tool (WAT039802) remove the upper and lower seals in the seal body.

6.Wet the insertion tool with 100% methanol or compatible solvent and slide the replacement high pressure
seal (WAT034497) onto tool with spring facing up.

7. Thoroughly wet the seal and place the seal guide tool onto seal body.

8.Wet the seal body thoroughly and insert the replacement seal into the seal body with the seal springs
facing the seal body.
Rebuild Lower Seal Wash Body
9. Remove the filter retainer from the lower seal wash body and remove the needle wash filter.

10.Insert a new needle wash filter (700001318) into the retainer.

11.Screw the filter retainer into the lower seal wash body finger tight.
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©2007 Waters Corporation
8
Rebuilding The Seal Pack
4.Remove the short plastic tube from the lower seal wash body.

5.Place the lower seal wash body filter retainer side down and, using the plastic side of the seal removal tool
(WAT039802) remove the wash tube seal.

6.Wet the new wash tube seal (WAT270668) and seal wash body thoroughly with methanol or compatible
solvent and insert seal into the lower seal wash body, o-ring side facing out.

7.Insert the new alignment tube (WAT031956) into the seal.

8.Reassemble the upper seal wash body, the seal body, and the lower seal wash body.

Reinstall Seal Pack

9.Manually turn the motor shaft to position the needle so it extends above the injector baseplate.

10.Wet the needle and inside of frit retainer with 100% methanol then carefully slide the seal pack
assembly down over the needle.

11.Reinstall the three screws with washers that secure the seal pack to the injector baseplate and
tighten alternately.

12.Reinstall the injector assembly into the unit.

NOTE: Be sure to properly reconnect the sensor and motor cables.

5. Power up the 2695 and perform a seal pack calibration.

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©2007 Waters Corporation
9
 Original 2695 Injector

• Be sure to wet the Needle and

Seal Pack before assembling.
• Internal needle volume
approximately 80µL.

~2mm

.044”

60° .057”
.020”

.015”

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©2007 Waters Corporation 0
New Style 2695 Injector
Injector- 700002789
Injector Upgrade Kit- 700002790
Spare Seal Pack- 700002791

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©2007 Waters Corporation
1
Injector Removal

Remove/Install
Sample Loop
using 2- 5/16”
wrenches
through the slot
in the Injector
Assembly

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©2007 Waters Corporation
2
Seal Pack / Needle Removal

In all pictures, Old Design

on Left – New Design on
Right

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©2007 Waters Corporation
3
 Seal Pack Adjust
▪ The 2695 determines the top and bottom of the lower seal in
motor steps relative to the STREAM position. From these it then
derives the SEAL position.

▪ The correct needle position depends on the particular needle,

and seal pack assemblies. Any change in these assemblies will
therefore require a new adjust seals operation to reprogram the
2695 for the correct step counts.
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©2007 Waters Corporation
4
 Qualification

▪ Flow rate Accuracy

— Using a 10ML & 25ML Flask measure 0.5, 1.0 & 5.0mls/min
▪ GPV Testing
— Review procedure
▪ Injection Accuracy
— Fill a 2ml vial with pre-slit septum's with water
— Zero balance and record weight
— Perform 6 - 50µL injections
— Zero balance and record weight
— [(w1-w2) / 6] x 1000 = µL per injection
▪ Sample Heater / Cooler Temperature Accuracy
— Using a calibrated thermocouple, place over the center of
the carousel and close door.
Make sure probe does not make contact with any surfaces.

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©2007 Waters Corporation
5
Hands On exercise – PM

▪ Complete PM on 2695

o Rebuild Solvent Manager

• Check Valves
• Head Seals
• Seal Wash Seals
• In-Line Filter

o Rebuild Sample Manager

• Rebuild Seal Pack &
Needle
• Replace Syringe
• Rebuild V1
• Replace Battery

▪ Re-initialize
▪ Static Leak Test
▪ Seal Pak Adjustment

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6
Troubleshooting
Common Errors

©2007 Waters Corporation

 Clear Sequence

▪ Clear #0
—· clears out all memory buffers
▪ Clear #8 key
—· Clears buffer memory only
▪ Clear screen key 1
—· Clears all LIFE counts
▪ Clear Screen Key 2
—· Clears ERROR Log
▪ Clear Screen Key 3
—· Clears TRACE Log
▪ Clear “?” (Help)
— Prints instrument information to disk

Config.d Default.mt Gpvtest.m Life.da Trace.d

at h th t at

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©2007 Waters Corporation
8
 General Troubleshooting
▪ There is no instant cure for any problems encountered
— Good old fashion troubleshooting.
— Take your time to understand the problem.
— Check the simple things first.
— Understand chemistries, flow rates, solvent conditioning and etc.

▪ Use error codes as a reference

— Company Confidential

▪ Slower flowrate than programmed

— Measure battery - 3 volts?
— Proper contact

Battery error message, Clock Resets or Looses Memory

Effect: Instrument looses NV Ram memory
Discussion: Low battery voltage can cause odd behavior of
memory. Possible Causes:
1.Clean and adjust battery contacts.
2.Check the Battery Voltage, it should not drop below 2.8 VDC.
Replace the Battery if needed WAT080443.
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©2007 Waters Corporation
9
 Software Update Loading Errors

▪ Test function of disk drive:

— Create method, export method to disk, delete method from memory
then import method
— Change position of disk protection tab
▪ Program disk?
— Perform checksum on disk
— Try transferring all files to newly formatted floppy disk and retry
installation
▪ Errors from new CPU PCB.
— “Fatal error.”
– Immediately after installation or next power up.
— A “clear ram” must be performed on a fresh software load
immediately after loading software.
– A “clear ram” may be needed on software updates only if
error occurs.
– Back up programs before clearing ram.

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Software Update Loading Errors

Fatal Error, Snowy/Blank Display or Constant Beep

▪ Effect: Instrument does not initialize
▪ Discussion: Corruption can occur if a recycle of instrument power
occurs during firmware loading. Or loading firmware from a
corrupt firmware disk. Or loading firmware from a faulty disk
drive.
▪ Possible Causes:
▪ 1. Reset the CPU by the reset switch or Clearing the Ram Memory
(Clear-Zero sequence)
▪ 2. Ensure the Boot Proms and all CPU associated Cables are
seated/secured properly.
▪ 3. Check the 5VDC Power Supply Voltage during initialization, it
should not fluctuate or be below 4.85 VDC. Replace P/S if needed
WAT270923.
▪ 4. Check the Battery Voltage, it should not drop below 2.8 VDC.
Replace the Battery if needed WAT080443.
▪ 5. Replace the Boot Proms (WAT271250) and reload firmware.
▪ 6. Replace the CPU PCB (WAT270915) and reload firmware.

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©2007 Waters Corporation
1
 Solvent Delivery Issues
Solv delivery H/W Fault (Solvent Delivery
Hardware Fault)
Effect: Faulty component associated with Pump
▪ Bubble detected Driver PCB and related functions.
— Criteria:>650psi and C/D ratio >1.8
▪ Check: Discussion: Any component plugged into the
— Plunger Driver PCB can be related to the error
Tubing connections condition.
— Degasser
— Chamber efficiency (flow rate) Possible Causes:
— Stroke length not excessive 1.Check the 24VDC Power Supply Voltage. It should
— Seals, check valves not drop below 22.5 VDC. Replace P/S if needed
▪ WAT270923.
Loss of prime
— < 25psi system pressure 2.Check the continuity for the 7 Amp Fuse F1 on the
— Criteria: 50 cycles Plunger Driver PCB. Replace if it's open
WAT290188.
▪ Solvent delivery hardware fault
▪ Plunger homing overpressure 3.Consider a faulty Plunger Driver to H/C PCB Cable
— Transducer out of range (WAT271015).
4.Consider a faulty cable connection or Heater Cooler
Exhaust Fan (700001067).
5.Consider a faulty Heater Cooler PCB (700001065)
or Heater Cooler (WAT270804).
6. Consider a faulty Plunger Driver PCB
(WAT270919).
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©2007 Waters Corporation
2
 Static Leak Test Fails

▪ SLT is an automated ramp and decay for solvent delivery

system
— Accumulator ramps to 5000psi
— Primary ramps to 4000psi
— Refer to service note
▪ Criteria
— %Volume = <20% (compress solvent to pressure)
— Decay rate <300psi/min (measures decay)
▪ Check the obvious first
— 100% MeOH
— Degas solvent, run in continuous mode or 100% Sparge
— Prime system at slower flow and for longer
— Check fittings
— Use new SS pin plug
— Seals, check valves - re-energize seals
— Rinse and wipe off head face seals
— Check Prime / Vent Valve (may be rebuilt using WAT270936)
▪ Dead end at outlet of System Transducer

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3
 Sample Management Issues

▪ Compression test
— Ramp and decay for sample loop area
— Parameters set using methanol, 100ul sample loop and 250ul
syringe

— Parameters: pressure 2690 2695

– Compression volume 300psi 100psi
– Compression 20ul 20ul
– Decay 1.0 min 1.0 min
time
– Delta 50psi 50psi
decay

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©2007 Waters Corporation
4
Alternate Compression Parameters

• For systems containing a larger

Syringe,
– Refer to service note
Parameter Description Range Typical Value
Compression Sets the target pressure for the 30 to 300 psi 300 psi
Pressure
compression check (2 to 20 bar) (20 bar)
Compressible Volume Sets the maximum volume that
25 ul syringe the syringe is displaced to 1 to 15ul 15ul
250ul syringe produce the compression 5 to 30ul 20ul
2500ul syringe pressure 5 to 100ul 50ul
Decay Time Sets the amount of time that .01 to 5.0 min 1.0 min
the system is kept at the
compression pressure
Maximum Delta Decay Sets the maximum difference in 10 to 100 psi <50 psi
pressure between the (0.7 to 7 bar) (<3.45 bar)
beginning
See Service Noteand the end of the decay time
11/11/1999
Under
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2690 ©2007 Waters Corporation
5
 Compression Test Fails- What to Do ?

▪ System is low volume

— Leaks are not always visible
▪ Manually purge valves and syringe
— Observe syringe barrel during purge
▪ Be sure Degasser is on
▪ Check fittings, syringe, tubing
▪ Pin plug V3 outlet when compressing to verify or eliminate
V3 failure
— Observe waste tubing during compression test for obvious V3
failure
— New style V3 introduced – see Service Note:
– Improved V3 Solenoid for 2690

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©2007 Waters Corporation
6
 Sample Management- Issues
▪ LVI’s breaking
— Refer to release notes for specific vial info
— Program needle offset (method specific)
▪ Ghost peaks:
— Refer to modified frit service note
— Attempt all traditional methods first
— Modified frit retainer (WAT035362)
– Needle wiper to increase seal wash volume
– There are risks

See Service Note

06/24/1998
Under 2690 13
©2007 Waters Corporation
7
 Sample Management- Issues

▪ Seal Pack Geometry Fault:

— Indicates Injector Sensor Bracket may need adjustment
— Failed seal pack adjustment
— Top, bottom, width
▪ Test failed?
— 1000psi <3ml, prefer 1- 1.5ml/min
— Degas continuous
— Check sensor bracket height for 5/16”
— Test high pressure valves
— Rebuild S/P, energize seal pack seals

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8
Config.dat

InjectConfiguration.inputHoldPin2 OFF
InjMan.needleBottomSealPosition 124
InjMan.needleTopSealPosition 43
InjMan.sampleLoopVolume 100.000000
InjMan.sealCalState True
InjMan.syringeSize 250uL
life.minDriveSize 16384
log.minDriveSize 16384
Contains Complete needleMotor.gain -62.992126
Instrument needleMotor.offset 0
Settings needlePositioner.backLashLimit
5.000000
needlePositioner.factoryVialOffset 57.150002
needlePositioner.measureBackLash True
needlePositioner.proximityLimit 0.500000
needlePositioner.sensorSearchAfterStream False
needlePositioner.sensorSearchAtStream True
needlePositioner.serviceVialOffset 0.000000
needlePositioner.streamOffset 0.000000
needlePositioner.userVialOffset
0.000000
Options.has717Autosampler False
Options.hasBarcodeReader
False
Options.hasColumnManager
False Options.hasDissolution
False
Options.hasPumpSealWash ©2007 Waters Corporation
13
9
True
Trace Log

TRACE LOG: Trace Log

SERIAL NUMBER: MX6CM0459M
PRINTED: 06/24/00 07:18:45am • File may be
motor lost sync
50397196(0x301000c) unknown code - in "stdtrace"
requested by
Power On at 03,26,98, 11:27:33
Rev: 1.20 (Jan 09 1998 07:44:31)
Service
9607.02: <PumpMan>: MotionController::ErrOVR_PRSS_SD - in "PumpMan": Pump shutdown on HW overpressure Support
11272.87: <PumpMan>: MotionController::ErrOVR_PRSS_SD - in "PumpMan": Intake
11272.90: <PumpMan>: MotionController::ErrOVR_PRSS_SD - in "PumpMan": Pump shutdown on HW overpressure
Engineer
11393.63: <capRunner0>: pumpMan::ErrPRESSURE_NOT_ACHIEVED - in "PumpMan": SetPressure
11549.21: <capRunner3>: pumpMan::ErrPRESSURE_NOT_ACHIEVED - in "PumpMan": SetPressure
11826.30: <capRunner3>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::CheckCarouselWithDogPin
11826.33: <capRunner3>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::EngageCarousel
11826.37: <capRunner3>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::SelectSlot
11826.41: <capRunner3>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::llSelectVial
11832.77: <capRunner3>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::CheckCarouselWithDogPin
11832.80: <capRunner3>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::EngageCarousel
11832.84: <capRunner3>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::SelectSlot
11832.87: <capRunner3>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::llSelectVial
11832.91: <capRunner3>: VialMan::ERR_NO_CAROUSEL - in "SelectNextVial": SelectNextVial: Error from SelectVial().
11908.31: <capRunner0>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::CheckCarouselWithDogPin
11908.35: <capRunner0>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::EngageCarousel
11908.38: <capRunner0>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::SelectSlot
11908.42: <capRunner0>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::llSelectVial
11914.56: <capRunner0>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::CheckCarouselWithDogPin
11914.59: <capRunner0>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::EngageCarousel
11914.63: <capRunner0>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::SelectSlot
11914.66: <capRunner0>: VialMan::ERR_NO_CAROUSEL - in "vialMan": VialMan::llSelectVial

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 Frequent Service Calls/Faults

Carryover???

Contamination???

Failing Compression
Check???

Adjusting Seals Failed???

Leaking Seal Pack???

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©2007 Waters Corporation
1
 Frequent Service Calls/Faults

VTRX Fatal Error After Installing New CPU???

Solvent Delivery Hardware Fault???

Column Heater Failure???

Static Leak Failing???

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©2007 Waters Corporation
2
Alliance Separations
Module
2795/2796
Overview

©2007 Waters Corporation

 2795 Features

▪ 5 Channel inline degasser

▪ Fixed or Variable Injection
▪ Flexible sample well plate format
— Not interchangeable w/ACQUITY UPLC™
▪ Independent needle wash / purge
— Plunger Seal Wash
▪ Sample Heater / Cooler
▪ Low dispersion fluidics
— ~400µL

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©2007 Waters Corporation
4
 Options

¡ 2795HT ▪ Field Installable Column

— Syringes - uL Switching Valves
o 500 - standard — 2 Position
o 100, 250, 1000, 2500 Regeneration
— Sample Loops - uL — 3 Position Selection
o 50 - standard — 6 Position Selection
o 5, 20, 100, 500, 2000
— Holding Loops - uL
o 128 - standard
o 500, 3000
— Bar Code Reader
Upgrade
— Weight = 136lbs
— Volume = <400µL
— # Samples = 1536

▪ Column Heater
— Without Valve (default)
▪ Column Heater/Cooler

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©2007 Waters Corporation
5
 HT Theory Review

Exploring HT Technology
▪ Sequential processing
— Traditional gradient chromatography, all operations are performed in
sequence
▪ Parallel processing
— Some operations are performed concurrently while run completes
o Results of first injection will vary from subsequent injections
• Timing will differ
• Not coordinated with previous injection
o Discard first of series of injections

▪ Parallel with Rapid Equilibration

— Some operations are performed concurrently, the column taken off
line, system equilibrates at a high flow rate

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©2007 Waters Corporation
6
Alliance Solvent Conditioning
®
2795

©2007 Waters Corporation

Alliance Start Up Flow Chart
2795

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©2007 Waters Corporation
8
 Dry & Wet Prime

▪ Use “Dry” Prime when the system is dry.

▪ Use the “Wet” Prime to change solvents or if instrument has

been idle for a long period of time.
— Run slow flow following to replace with degassed solvent.

▪ Complete Remaining Prime Functions.

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©2007 Waters Corporation
9
 System Prep
After manually performing a dry prime, The 2795 System
Prep function performs the following functions:
▪ Fills the fluidic pathway with fresh eluents, according to the
selected separation method.
▪ Performs a wet prime of 100% of each solvent used by the
method, and finishes by setting the instrument at the initial
composition.
▪ While priming, it washes the needle and purges the
syringe.
— An optional extension of the solvent priming and
the syringe purge time can be specified,
o useful when priming in the morning or after
extended periods of system inactivity.

▪ When you select the System Prep function immediately after

the powering up the instrument, a message on the Status
screen reports that the System Prep function requires 5
minutes for the degasser to condition the eluents before
initiating the System Prep function.

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©2007 Waters Corporation 0
 2795 External Tubing
• Needle Wash Solvent: (A.K.A. Strong Solvent)
– Use “Prime Needle Wash” diagnostic
– Typically stronger than sample and mobile phase to dissolve sample
residue.
– Flushes internal and external portion of the needle preventing carryover.

2795 Only

• Needle Purge Solvent:

– Use “Refresh Syringe” Direct Function
– Degassed for good hydraulic properties.
– Purges needle, holding loop and syringe fluid path.
– Must match the initial mobile phase gradient condition
▪ Some does get injected with sample during partial loop
injections.

15
©2007 Waters Corporation
1
 Refresh Syringe

▪ Primes Syringe to remove air

▪ Replacement Volume
recommended
2.5 times loop volume plus
needle volume
▪ Set to:
— 10 Strokes
— 600uL Volume

• Automated routine performed

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©2007 Waters Corporation
2
 Calibrate Needle to deck

▪ Perform with the “Change

Needle” Diagnostic
▪ Sets needle tip to carrier plate for
all vertical related dimensions
▪ If no error is displayed the test
passed
▪ Verify calibration using Change
Needle in Service Utilities

• Verify Deck Offset

• Deck Offset defaults to 60
• Indicator calibration not performed
• Typical Deck Offset
• Low to Mid 50’s

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©2007 Waters Corporation
3
 2795 Diagnostics

May be used to troubleshoot

Injector Fluid Path

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©2007 Waters Corporation
4
 – Start-Up Hands On Exercise

▪ Connect all power and fluidic

connections
▪ Perform: as applicable
— Dry prime
– Wet prime
— Static leak test
– Try only once
– Refresh Syringe
– Prime Needle Wash
– Calibrate Needle to Deck

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©2007 Waters Corporation
5
2795 Injection Flow Path
Injection Sequence

©2007 Waters Corporation

 Component Layout

Waste Bypass
▪ Sample Management Components Power valve
valve
— Valves supply
Wash
o Purge, Needle Wash, Bypass, Waste
pump
— Sample Management Pumps

Fluidics Driver
o Needle Wash Drain
o Drain Pump pump
— Syringe Drive
X

PCB
o High speed, new P/N for 2795 only

CPU PCB
▪ Electronic Components
— Fluidics Driver PCB XYZ
o LED readout / Opto-sensor feedback mechanism
o Smart connectors
• Motors, J11 - J14
• Opto-Sensors, J15 - J 18 Carrier Y
• Solenoid Valves, J2 - J5 motor
— 600W Power Supply
Wash
valve
Purge Lock Pin
valve motor
Syringe
drive

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©2007 Waters Corporation
7
 Sample Aspiration

• Needle at well
position
• Wash Valve open
– to Purge Valve
• Purge Valve open
– to Syringe
• Syringe draws
sample into holding
loop
• Sample Loop offline
– Connect to waste

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©2007 Waters Corporation
8
 Sample Loading

• Needle at inject port

• Syringe moves
sample into sample
loop

• Excess flushes out

to waste

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©2007 Waters Corporation
9
 Sample Injection

• Sample loop
switched on line with
column

• Flowrate moves
Sample through
column

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©2007 Waters Corporation 0
 Internal Wash

• Internal Needle
Wash and Inject Port
Wash

• Needle wash
pumped through
holding loop, needle
and inject port

Also known as
a Vent Valve

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©2007 Waters Corporation
1
 External Wash

• Needle raises, washes

outside of needle,
excess flows out drain
• Drain pump dries
port area
• Syringe refills system
with degassed
solvent

Drain Pump
turns on after
external wash is
complete

16
©2007 Waters Corporation
2
 Purge Solvent

• Purge solvent
flushed through
holding loop to
refresh system

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©2007 Waters Corporation
3
 Refresh Syringe

• Syringe returns to
home position
preparing for next
sample
withdrawal

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 Partial Loop Injections

▪ Centers sample in loop

▪ Air gaps reduce sample dilution
Purge Solvent — 3uL default
▪ < 50% of loop size
— minimize laminar flow effect
Air Gap — Sample is centered in loop
▪ Purge solvent also gets injected
Sample for partial loop
— match initial gradient
Sample

With a 50µL loop: Full

Air Gaps
Loop 75µL after
loop

Mobile Phase 50 µL in loop

Purge Solvent
75 µL before
loop Total 200 µL 16
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Important Note: Parallel Processing

0 1 2 3 4 5 6
MINUTES

▪ Discard first of series of injections

— Timing will differ
— Not coordinated with previous injection
— Results of first injection will vary from subsequent injections

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2795 HT Separations
Module
Component
Details

©2007 Waters Corporation

 Unpacking the 2795

Attention:
Remove the foam
2 shipping
restraints
before power up
1
3

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 Waste Tubing

▪ Very Important all waste

tubing be properly
placed in the Main Waste
Tubing

Smooth walled
tubing now used in
place of
corrugated tubing
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 Valve Locations
Bypass
Valve
Waste
Valve
Needle Wash Pump

Purge & Wash Valve

Lock Pin Motor

Drain Pump

XYZ
Mechanism

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 Valve Function
▪ The Purge Valve operates with the wash valve during the wash cycle to allow the
needle and the inject port to be flushed with purge solvent in preparation for the
next injection.

▪ The Wash Valve operates with the bypass valve during the wash cycle to allow
the needle and the inject port to be flushed out in preparation for the next
injection.

▪ The Bypass Valve operates with the wash valve during the wash cycle to allow
the needle and the inject port to be flushed out in preparation for the next
injection.

▪ The Waste Valve opens to allow solvent to flow out to waste during the Refresh
cycle when the syringe is being filled with fresh solvent.

▪ The Needle Wash Pump is a solenoid which fires one pulse per second, like the
seal wash pump. It is isolated with well nuts to reduce noise.

▪ The Drain Pump is used to evacuate the waste liquid from the wash station after
a needle wash so that it does not re-contaminate the needle.

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Fluidics Driver PCB Connectors

• LED’s for syringe, carrier

and interlock devices
• Full description in
ESM

• Smart connectors
▪ Motors, J11 - J14
▪ Opto - Sensors, J15 - J 18
▪ Solenoids - Valves, J2 -
J5
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Safety Interlock Switches

▪ 1/10 speed
— Top or right
panel removed

▪ Bypass with
magnet
— Or install jumper across
top and bottom pins of
connector J15-J18
(second pair from left)

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 Sample Loop installation

¼” Slotted Wrench

Injector / Sample Loop area

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 Sample Loop

▪ To reduce likelihood of void

volumes, Label tube ends if
Sample Loop is removed

▪ Rheodyne’s tolerance
specification is +/- 0.030”
from port to port

Void Volume

Tubing End

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 XYZ LED’s and Mechanism

DS13 Needle
Bottom

DS14 X Home
DS15 Y
Home DS16
Z-Home

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 Needle Bottom Sensor

▪ Bottom edge of Flag should be even

with the Bottom edge of Sensor
— Adjust so when needle is up, out of
the inject port, flag is even with or
slightly above bottom edge of
sensor

PCB
Flag
Sensor

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Plate Positioning

Pushes Well
Plate to A1
corner post

Secures
carrier plate

New Spring

Old Spring

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Injector

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Injector Port

Old Style Inject Port:

• Varied 10µL – 22µL
• 1 machined ring at top

New Style Inject Port:

• Aprox 12µL
• 2 machined rings at top

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 2796 (Inert version of 2795)

▪ Wetted materials
— Titanium, PEEK, Teflon, UHMWPE
▪ Fittings
— Compression screws – Treated Stainless Steel
— Ferrules – PEEK or LT-135X (SS lock ring/PEEK)
▪ Sample Loops
— Titanium
o 10ul, 20, 50ul, 100, 200, 500, 2ml
— PEEK
o 5ul, 20, 50, 100, 200, 500, 2ml

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 2796 Solvent Management
Assembly

▪ Pump Head
— Universal design
— Titanium Heads and CV

▪ Inline Filter
— Titanium housing
— UHMWPE insert

▪ Plunger Seal Wash

— Program cycle time
increased
— Ferrules - 1/8”
reverse
— Comp Screw –
1/8” Peek

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2796 Head Assembly

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 2796 “Service Friendly” Titanium Injector

Identify by “Ti” on the 8 o’clock

position of the valve

Added extension cable for ease

of removal
Added access door to right
panel Positioned at 90 degree
offset

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 Hands on Exercise - Main Components

▪ Remove, Inspect, Reinstall all components

— Remove Solvent Management system
o Remove Solvent Management Tray
o Remove Solvent Conditioning Tray

— Remove 2795 Sample Mgmt system

o Remove the XYZ Assembly

— Remove and review PCB’S and Power

Supply Assy.

Use Caution when handling

printed circuit boards

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2795 HT Programming

©2007 Waters Corporation

 Quick injection programming

Press Menu/Status

Press Direct Function

Press Inject Samples

Set parameters
Press OK

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 Creating a Separation Method

A 4x overfill will pull 4x loop

volume into needle & center
in sample loop

Recommend
Pre and Post
Air Gaps of
3.0µL
Runs once for
each plate or
well. Rewrites
plate
1 – 2 mm measurements in
Recommended
custom plate

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 Creating a Sample Set

▪ Sample Set
— Program parameters for injection. Including sample volume,
run time, etc.

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 Creating a Custom Plate

▪ Custom Well Plate

— Must be created when other than a Waters well plate is used

— Waters well plates are pre-programmed choices in the plate

list

Plate measurements must be 3 to 4mm

of actual size to use Seek Well Bottom
and Check Plate Height
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 Plate Parameter Definitions

Vertical (mm)
Well A1 offset (mm) Well depth
Rear
1
A Plate height
Left 2

Row-to-row Vial Offset

B

Col-to-col
Spa MassLynx:
cing (mm) Height = Plate size Z
Depth = Vial depth
Ma Offset = Draw depth in Inlet
ssLynx: Autosampler Parameters
Left
= Top left vial X
Rear = Top left vial
Y

Deck Offset:
From carrier to
bottom tip of needle

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Hands on Exercise - Programming

Familiarize yourself with the

screens
▪ Direct Functions

— Create Methods
o Sample and Instrument
o Make an injection

— 2795 Custom Plate

▪ Service Diagnostics

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2
 Alliance Service
—PM
® Procedures
Procedures
oPM Test
—Calibration
Protocol

©2007 Waters Corporation

 2795 HT
Sample
Manager

©2007 Waters Corporation

 Performance Maintenance

¡ 2795 PM Kit P/N 201000107

— PM base at 20,000 injections

• Injector Rebuild Kit • Plungers

• Inject Port Seat • Head Plunger Seals

• Head Face Seals
• Port Seal Washer
• S/W Tube Seals
• Needle
• S/W Tube Seals
• Peek Ferrule
• Check Valves
• Syringe, 500ul • Solvent Sink Filters
• S/W Face Seals • Battery
• Filter Insert • Protocol and PM
Label

• = Specific to 2790/95 only

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 Performance Maintenance Visit Features

▪ During the Performance Maintenance Visit the FSE will

check whether the current version of firmware is installed
— If the instrument is not at current revision, the FSE consults
with customer to advise about the features/benefits of the
latest version
— The FSE reviews the error log (Alliance) or trace log (ACQUITY)
for potential issues
o During a corrective repair event the FSE can review the logs to help
troubleshoot the issue

Dust Kit 700002665

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 Injector Removal

▪ Remove the Torx screw

from the rear bracket

▪ Loosen the reverse

threaded screw from Wash
Station to drop injector
— Access from side
— Disconnect Tubing from
front

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 Rheodyne Injector Rebuild

Replace:
• Rotor Seal Rotor Seal

• Stator Face Stator Ring

Stator Ring
• Not part of Screws
(not in all models)
PM Kit
Stator Face Assy.
(some models have 2 pins
Stator Stator
Screws
WAT072638
Stator
Screws

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Needle Removal
▪ Disconnect the holding
loop

▪ Loosen the two captive

screws to remove the clamp

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Needle Rebuilding

▪ Using a 5/16” wrench onto the compression screw

and a 3/16” wrench onto the housing remove/replace
the Needle

▪ Pay close attention to:

— The order in which the components are installed onto
the needle
— The orientation of the sensor flag should be pointing in
the upwards direction
— Ensure components are free moving and not binding

▪ Only new needle in kit.

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XY Needle Alignment

• Alignment Tool
▪ To align needle with
needle port – P/N
— Has little effect on well 700000986
positioning

▪ Use MOTORS XYZ OFF

— In Service Utilities

▪ Install XY Alignment
Tool
— Align to key hole slot at needle
port

▪ Position sensors

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X Sensor and Flag

▪ Position XY Tool into key hole

slot
— Move sensor left
— Verify that LED flashes

▪ If LED does not flash, a

sensor adjustment is needed

Push Here while watching LED DS14

DS13 Needle Bottom

DS14 X Home
DS15 Y Home
DS16 Z-Home

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Y Sensor and Flag

▪ Leading edge
— relates to inject port
— Move sensor right
— Verify that LED flashes

▪ If LED does not flash, a

sensor adjustment is
needed

DS13 Needle Bottom

DS14 X Home
DS15 Y Home
DS16 Z-Home Push Here while watching LED
DS15

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Compression Test Diagnostic

▪ For troubleshooting sample management

▪ Located in Service Utilities

Compression Test Kit
▪ Not a qualification tool

▪ Field service use only Cable

— Not available to Transducer 270906
270966
customers

Compression fittings
1/4” and 1/16”
Tube
.020” x 7.0”

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 Compression Test Diagnostic

• Plumb transducer
to existing purge CPU
valve tube

• Plumb jumper tube

to the syringe
Tubing Cable

• Connect Cable to
CPU sample
connection

• Access Comp
Test in service
diagnostics

• Pin plug at specific

places to identify
bad component

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 Qualification

▪ Flow rate Accuracy

— Using a 10ML & 25ML Flask measure 0.5, 1.0 & 5.0mls/min
▪ GPV Testing
— Review procedure
▪ Injection Accuracy
— Fill a 2ml vial with pre-slit septum's with water
— Zero balance and record weight
— Perform 6 - 50µL injections
— Zero balance and record weight
— [(w1-w2) / 6] x 1000 = µL per injection
▪ Sample Heater / Cooler Temperature Accuracy
— Using a calibrated thermocouple, place over the center of
the carousel and close door.
Make sure probe does not make contact with any surfaces.

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Hands On exercise – PM

▪ Complete PM on 2795

— Rebuild Solvent Manager

o Check Valves
o Head Seals
o Seal Wash Seals
o In-Line Filter

— Rebuild Sample Manager

o Replace Needle
o Replace Syringe
o Rebuild Injector & Needle
Port
o Replace Battery
o Align X & Y Axis Sensors

▪ Re-initialize
▪ Static Leak Test
▪ Calibrate Needle to Deck

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 Common
Troubleshooting

©2007 Waters Corporation

No instant cure for problems

▪ Good old fashion troubleshooting

▪ Take your time to understand problems

▪ Check simple things first

▪ Understand chemistries, flow rates, solvent

conditioning, etc.

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Troubleshooting

▪ Poor injection reproducibility

— Use known
chemistry/settings
— Use default parameters
o Air gaps to large?
- compressible
purge solvent
o Air gaps to small? - sample
dispersion
— Perform Sequential Mode
operation
o Don’t use Parallel mode or
Rapid Equilibration
— Perform Full Loop Injections
o Sample loop vs. sample
size?
— Weigh vial before and after
injections
o Verifying aspiration of
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Troubleshooting

▪ Poor injection reproducibility

— Needle
o tip dirty, bent, restricted
— Needle Port Interface /
Injector Flow Path
o seal interface worn,
port/flow paths restricted
o alignment possible
— Compression fittings &
connections leaking
o Run NW prime diag and
watch wasteline for vacuum
lock
— Syringe worn out, valves
failed
o Verify flow/loading by
monitoring waste tube
— Wells covers or septas

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Troubleshooting

▪ Sample Carryover
— Wash solvent compatibility
o verify solubility
o Increase timing or change sequence
— Wash pump not primed
o listen for low tone click
o measure ~ 2.5mL/min flow rate
— Needle tip damaged, restricted or
contaminated

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Troubleshooting

▪ Needle Malfunction Errors

— Well Depth Error (error occurs when needle is at the plate)
o Verify plate type and dimension
o Check plate for crowning
o Add 1-2 mm offset from bottom of well
— Bottom Sensor Error (error occurs when needle is at the injector)
o Check sensor / flag function
o Try re-initializing ( if “bottom sensor error”)
• Needle assy. not mounted properly
• Bent needle

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Troubleshooting

▪ Y Axis Error / X Axis Error – service note

▪ Caused by “BINDING” of mechanism
— Dust on rails
— Mechanism hitting something
— Loose belt clamp, belt slipping
— Loose pulley on motor
— Cable in the way
▪ Alarm Messages
— see ESM for listing
— see Operators Guide for listing
▪ Troubleshooting Guide
— see handouts
— future service note

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4
Common Error Messages

▪ Plunger Homing Over Pressure

— Restricted Inline Filter
▪ Loss of Prime (<25psi for 50 cycles)
— Solvent lines went dry or pump not primed
— Failed Check Valves
▪ Bubble Detected (C/D Ratio above 1.8)
— Pump not primed properly
— Tubing connections
— Degasser problem or chamber efficiency
— Stroke length
— Seals or Check Valves failed

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5
Optimizing Injections

▪ Improve repeatability
— Sample loop, first choice, versatile option
— Full loop injections, better repeatability
— Smaller injections
o smaller sample loop / smaller syringe
o < 50% of loop size, minimize dispersion
o but…adds to cycle times by requiring more syringe draws to
purge/wash needle

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Loop Recommendations

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 Frequent Service Calls/Faults

Needle Malfunction (Bottom Sensor)???

Y or X Axis Malfunction???

Injector Movement Failure???

Carriage Malfunctions???

Reproducibility???

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 Frequent Service Calls/Faults

Retention Time Shifting???

Degasser Failures???

Fatal Error, Snowy Screen???

Injecting Into Wrong Well???

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