This document provides guidelines for safe injection and infusion practices to prevent transmission of infections. It outlines incorrect practices that can lead to transmission, such as using the same syringe on multiple patients or accessing a shared IV fluid bag or medication vial with a previously used syringe. The Centers for Disease Control and Prevention guidelines for injection safety are summarized. Proper infection control and aseptic technique, dedicating multi-dose vials to single patients, and never entering a vial with a used syringe are emphasized. Occupational exposures from needlestick injuries or splashes are discussed, along with the infections that can result and post-exposure management steps.