Downloaded 13 times
More Related Content
Similar to 2014 bgri de pauw presentation
More from Borlaug Global Rust Initiative
2014 bgri de pauw presentation
- 1. Evidence for Recombinationof Sr2 and Fhb1 #bgri2014 #Borlaug100 R.M. DePauw, R.D. Cuthbert, R.E. Knox, S. Kumar, A. Singh, A.K. Singh, H. Campbell, S. Bhavani, D. Singh, F.R. Clarke, and T. Fetch
- 2. Outline • Background ongenes Sr2 and Fhb1 • Objective: to generate evidence of recombination of Sr2 and Fhb1 resistance from repulsion to coupling • Genetic Materials: Carberry/AC Cadillac • Evidence: – Pedigree – Phenotype – FHB resistance with PBC and stem rust resistance – Haplotype 2
- 3. Background: Sr2 – Durableadult plant stem rust resistant gene – Partial resistance – Enhances expression of other Lr and Yr rust resistance genes – 3BS (Yaroslav emmer to Hope and H.44-24 etc) – QTL analysis of stem rust co-segregation with (microsatellite) markers in the region of gwm533 and gwm493 – SNP marker XcsSr2 co-segregated with Sr2which is closest reported marker to date (Mago et al. 2011) – Tightly linked to PBC (Kota et al. 2006) – Fine mapping studies have identified recombinants in the region of gwm533 and gwm493 (Kota et al. 2006, McNeil et al. 2008)
- 4. Background: Fhb1 – Confersresistance to FHB, a major fungal disease causing loss of yield and grain quality (mycotoxins harmful to human and animal health). – Resistance on 3BS (from landrace Taiwan wheat to Sumai 3). – QTL analysis of FHB detected co-segregation with (microsatellite) markers in the region of gwm533 and gwm493. – Current most tightly linked markers are Xsnp7 and Xsnp11, Xsts256, and Xsts3B-138 (Bernardo et al. 2012) – Fine mapping studies have identified recombinants in the region of gwm533 and gwm493 (Cuthbert et al. 2006, Bernardo et al. 2012).
- 5. Background: • Sr2 andFhb1 are linked with QTL in the Sr2/Fhb1 region of gwm533 and gwm493 on 3BS. • ~ 5 cM apart (Bernardo et al. 2012) • Sr2 and Fhb1 are currently reported only in repulsion.
- 6. 6 3B 3BL-2 0.22 3BL-3 0.41 3BS-10.33 3BS-8 0.78 3BS-3 0.87 3BS-2 0.56 3BS-5 0.20 3BL-11 0.81 3BL-7 0.63 3BL-10 0.50 3BL-8 0.25 3BS-6 0.54 Marker Start (bp) End (bp) Amplicon csSr2 -‐ -‐ 172/112 225/112 gwm389 -‐ -‐ 139/115 gwm533 -‐ -‐ 118/142 Sr2/Pbc ctg0011 (1.2 Mb) CA746621 393632 394412 761 3B042G11 398244 398079 183 CD882879 468666 468473 213 BEX_2 562544 562042 522 3B028F08 564671 564447 243 CA640157 1013696 1014177 482 Xsnp3BS-‐2 1103249 1102799 470 Fhb1 ctg0954 (3.1 Mb) STS3B-‐142 1579003 1578866 157 STS3B-‐206 1794973 1795201 247 cA6078 2162814 2162981 187 STS3B-‐138 2193637 2193343 314 snp3BS-‐4 2460842 2460286 578 STS3B-‐80 2461261 2461081 200 cA6059 2463669 2463870 221 umn10 (snp3BS-‐11) 2467047 2466838 229 cA6061 2839954 2840202 268 gwm493 -‐ -‐ 195/138
- 7. Norman Borlaug, 1970Combine Sr2and FHB Norman Borlaug,
- 8. Objective: • To identifya recombinant of Sr2 and Fhb1 in coupling from the doubled haploid population of Carberry/AC Cadillac • Null Hypothesis: PBC(Sr2) and low FHB will not be expressed together in a DH line • Evidence for recombination: a DH line which expresses both PBC and low FHB symptoms
- 9. Parental Materials • ACCadillac – resistant to stem rust – moderately susceptible to FHB – expresses molecular marker products linked to Sr2 but not to Fhb1 resistance • Carberry – resistant to stem rust – moderately resistant to FHB – displays molecular marker products linked to Fhb1 but not to Sr2 resistance • Both parents have gene Lr34/Yr18/Sr57/Pm38/Ltn1 (7DS)
- 10. Genotyping of thepopulation • Carberry/AC Cadillac – Phenotyped 261 DH line subset of doubled haploid population of 812 lines • Genotyped with DArT® (578), SSR (54), BAC-derived (3), CAPS (3), and STS (3) molecular markers including: • SSR marker Xumn10 (SNP: Xsnp3BS-11) is reported to be most diagnostic for Fhb1 (Bernardo et al. 2012) • XcsSr2 is reported to be most diagnostic for Sr2 (Mago et al. 2011)
- 11. Evidence: Pedigree AC Cadillac Carberry Thatcher Hope H44-24 Marquis Yaroslav Emmer Sumai #3 AC Cadillac 1.00 Carberry 0.16 1.00 Thatcher 0.60 0.23 1.00 Hope 0.16 0.15 0.25 1.00 H44-24 0.16 0.12 0.25 0.50 1.00 Marquis 0.31 0.18 0.50 0.50 0.50 1.00 Yaroslav Emmer 0.01 0.05 0.00 0.50 0.50 0.00 1.00 Sumai #3 0.01 0.03 0.00 0.00 0.00 0.00 0.00 1.00 • Both AC Cadillac and Carberry have Yaroslav emmer in their genealogy • Only Carberry has Sumai #3 in genealogy
- 12.
- 13.
- 14. Evidence: Stem Rustand Genetic analysis Stem rust field nurseries: • Njoro, Kenya: 2010, 11, 12, 13 • Swift Current, Canada: 2011 and 2012, and 2013 Stem Rust QTL (Singh et al. TAG 2013) • AC Cadillac contributed stem rust resistance QTL on chromosomes 2B, 3B, 5B, 6D, 7B and 7D • Carberry contributed resistance QTL on 4B and 5A. • Epistatic interactions were observed between loci on 6D and 3B
- 15. Yellow Rust • SwiftCurrent, Canada in 2011 • Lincoln, NZ: 2012, 2013 • Njoro, Kenya: 2009, 2011 Yellow Rust QTL • AC Cadillac was a source of yellow rust resistance QTL on chromosomes 2A, 2B, 3A, 3B, 5B, and 7B • Carberry was a source of resistance on chromosomes 2B, 4B, and 7A. (Singh et al. 2014) Evidence: Yellow Rust and Genetic Analysis
- 16. Co-location: • Yellow rustresistance QTL co-localized with stem rust resistance QTL on 2B, 3B, and 7B. • Sr2 and Yr30 reported in same region 3BS Evidence: Genetic analysis
- 17. Epistatic Interactions: • Severalepistatic interactions were identified both for yellow and leaf rust resistance QTL Trait Environment QTLa 1 QTLb 2 Yr severity Kenya QYr.spa-3B .1 QYr.spa-2B Lr severity Canada QLr.spa-3B QLr.spa-4B Yr infection response New Zealand QYr.spa-3B.1 QYr.spa-2A Yr infection response New Zealand QYr.spa-3B.1 QYr.spa-7B.2 First QTL1 Second QTL2 Source: Singh et al. TAG 2014 (in press) Evidence:
- 18. Evidence: Fusarium HeadBlight • FHB nurseries • PLP 2010, 2011, 2013 • Variables: Incidence, Severity: Disease Index = (Incidence * Severity)/100 • QTL in AC Cadillac 4B • QTL in Carberry 3B, 5A, 6D
- 19. Evidence: Pseudo BlackChaff • PBC is a dark pigmentation that may occur on the glumes, peduncle and below stem internodes, but its level of expression varies with genetic background and environment. • Linkage of Sr2 and PBC has not been broken in about 90 yrs • PBC scoring: an expression in 6 nurseries out of 12 • QTL for pseudo-black chaff: – AC Cadillac 2B and 3B – Carberry 6A
- 20. Pseudo Black Chaff Carberry/ACCadillac DH line B0767&AB019
- 21. 6 4 2 0 0 25 50 75 100 FHB Index Pseudo black chaff (#Environment) 1 2 3 4 5 6 7 8 9 11 12 Color code No. of lines Carberry AC Cadillac B0767&: Scatter plot of 261 DH lines
- 22. Pseudo Black Chaffand low FHB
- 23. Genotypic Data Phenotyping Carberry/ACCadillac: Haplotpye + Phenotype Sr2/Pbc Fhb1 gwm389 csSr2 3B028F08 3B042G11 gwm533 wPt-7341 wPt-8855 wPt-2757 wPt-8446 wPt-1620 wPt-7984 BArc75 wPt-3260 wPt-744251 wPt-1484 wPt-10972 wPt-9066 Barc147 sts3B-142 UMN10 FhbIndex PBC(#Environment) SeveritySr InfectiontypeSr AC Cadillac A A A A A A A A A A A A A A A A A A A A 31 6 3 R Carberry B B B B B B B B B B B B B B B B B B B B 22 1 5 R B0767&AT073 B B B A A - B B B B B B B B B B B B B B 15 1 3 R B0767&BF021 B B B A A B B B B B B B B B B B B B B B 12 2 10 R B0767&BF115 B B A A A B B B B - B B B B B B B B B B 5 1 15 R
- 24. Summary of evidence • Carberry/AC Cadillac: 261 DH phenotyped • Evidence provided for Sr2 and FHB1: pedigree, phenotype, genotype • DH lines expressed variation for: FHB; Stem, Yellow, & Leaf rust resistance; PBC • QTL on 3B detected for FHB; Stem, Yellow, & Leaf rust resistance; PBC • Co-location of QTL for Sr and Yr and Epistatic interactions Sr and Yr, and Sr and Lr • Recovered recombinants with PBC and FHB resistance • Haplotype recombination in Sr2/Fbh1 region based on markers reported to be closest linked
- 25. Acknowledgements • Colleagues andSupport Staff at: • AAFC, Swift Current, Lethbridge and Winnipeg, Canada • CIMMYT, Kenya • KARI, Njoro, Kenya • Plant and Food Research, Lincoln, New Zealand • Funders: – levy by Farmers of western Canada administered by Western Grains Research Foundation – AAFC: Growing Forward and core funding
- 26. Yield trial plots, KARI,Njoro, Kenya, 1971 Photo Credit: R. M. DePauw