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- 1. Bacterial Classification and Nomenclature Faculty:Dr. Rakesh Sharda
- 2. ✓ Taxonomy isthe science of the classification of organisms, with the goal of showing evolutionary relationships among organisms. ✓ Taxonomy includes: (a) Identification. (b) Nomenclature. (c) Classification. What is Taxonomy
- 3. Identification – isthe process of studying and recording the identical and distinguishing features Nomenclature – is the process of assigning names to the various taxonomic ranking of each living organism. Classification – is the orderly arrangement of organisms into groups, preferably in a format, that shows evolutionary relationships.
- 4. Basis of Taxonomy ✓Pheneticsystem groups organisms based on mutual similarity of phenotypic characteristics. May or may not correctly match evolutionary grouping, e.g. motile v/s non motile bacteria ✓Phylogenetic system groups organisms based on shared evolutionary genetic heritage.
- 5. Taxonomy of bacteria ✓Livingorganisms were once divided into only two kingdoms: plantae and animaliae: bacteria, fungi, and algae were classified with plants; protozoa were classified as animals. ✓Haeckel in 1865 proposed third kingdom Protista for unicellular microorganisms such as bacteria, algae, fungi and protozoa. ✓ Bacteria were separated into the Kingdom Procaryotae or Monera in 1969 by Whittaker – five kingdoms
- 6. Whittaker 5 Kingdom classification Basedon cellular organization and nutritional patterns: Procaryotae or Monera - prokaryotic, unicellular, variable nutrition patterns – bacteria. Protista- eukaryotic, unicellular or colonial, heterotrophic – protozoa, slime molds, some algae. Myceteae or Fungi - eukaryotic, unicellular or multicellular, heterotrophic or absorptive – molds, yeasts, mushrooms. Plantae - eukaryotic, multicellular or colonial, autotrophic – plants, some algae, mosses, ferns. Animalia - eukaryotic, multicellular, heterotrophic or ingestive – animals.
- 8. The Three Domains (CarlWoese and George Fox, 1977) Currently, living organisms are divided into three super kingdoms, called as domains, ✓Eukarya Domain include plants, animals, fungi, and protists ✓ Eubacteria Domain include bacteria with peptidoglycan in cell wall. ✓Archaea Domain include bacteria(Archaea) with unusual cell walls
- 9. ➢Based on cellularrRNAs sequence primarily ➢cell membrane lipid structure and sensitivity to antibiotics. ➢rRNA molecules throughout nature carry out the same function and genetically stable very little over time. ➢This system proposes that a common ancestor cell ("Cenancestor") gave rise to three different cell types, each representing a domain.
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- 11. The Archaea (archaebacteria) •Archaeaare prokaryotic cells. •contain rRNA that is unique to the Archaea distinctly different from the rRNA of Bacteria and Eukarya. •cell membranes composed of branched hydrocarbon chains attached to glycerol by ether linkages. •cell walls of Archaea does not have peptidoglycan. •Archaea are not sensitive to some antibiotics that affect the Bacteria, but are sensitive to some antibiotics that affect the Eukarya.
- 13. The Bacteria (eubacteria) •Bacteria are prokaryotic cells. • contain rRNA that is unique to the Bacteria distinctly different from the rRNA of Archaea and Eukarya. • celll membranes composed of unbranched fatty acid chains attached to glycerol by ester linkages . • cell walls contain peptidoglycan. • Bacteria are sensitive to traditional antibacterial antibiotics but are resistant to most antibiotics that affect Eukarya.
- 14. The Eukarya (eukaryotes) •Eukarya have eukaryotic cells. • contain rRNA that is unique to the Eukarya distinctly different from the rRNA of Archaea and Bacteria. • Cell membranes composed of unbranched fatty acid chains attached to glycerol by ester linkages . • Not all Eukarya possess cell wall, but those having a cell wall does not contains peptidoglycan. • Eukarya are resistant to traditional antibacterial antibiotics but are sensitive to most antibiotics that affect eukaryotic cells.
- 15. Methods of IdentifyingBacteria ✓ Morphological characteristics ✓ Biochemical tests ✓ Serological tests ✓ Phage typing ✓ Sequences of amino acids in proteins ✓ Percentage of G-C pairs in the nucleic acid ✓ Number and sizes of DNA fragments produced by restriction enzymes ✓ Sequence of bases in 16S rRNA (Ribotyping)
- 16. Nomencalture of Bacteria ScientificNomenclature ✓ According to scientific nomenclature, each bacteria is assigned two names (binomial nomenclature): a genus and a species. ✓ Rules for the assignment of names to bacteria are established by the International Committee on Systematic Bacteriology.
- 18. Linnaean hierarchy Example forE.coli Domain: Eubacteria Kingdom: Procaryotae Division/Phylum: Gracilicutes Class: Scotobacteria Family: Enterobacteriaceae Genus: Escherichia Species: coli
- 19. Methods of ClassifyingBacteria The Intuitive Method In this method a microbiologist who is thoroughly familiar with the properties of the organisms decides that a particular organism represent a species or genus.
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- 21. Numerical taxonomy In thismethod of taxonomy of many (100 to 200) characteristics for each bacterial strain is determined, giving each characteristic equal weight. Then percentage similarity (%S) of each strain to every other known strain is calculated by the formula: %S = ND/NS +ND where, NS= number of characteristics that are same (positive or negative) for the two strains, ND = number of characteristics that are different. strains having a higher %S to each other are placed into same group
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- 23. Genetic Relatedness ➢most reliablemethod of classification ➢based on the degree of genetic relatedness between organisms ➢relies upon G+C content, DNA homology, ribosomal RNA homology (16S rRNA for prokaryotes and 18S rRNA for eukaryotes – ribotyping).
- 24. Genetic Homology Genetic homologies (similarities)can be determined by looking at: • Base compositions • DNA-DNA or DNA-RNA Hybridization • DNA or RNA sequencing
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- 26. The Bergey’s Manual Bergey’sManual of Determinative Bacteriology ✓International standard for bacterial taxonomy. ✓First published in 1923. ✓9 editions have been published. ✓ From 1980 it is renamed as Bergey’s Manual of Systemic Bacteriology published as a 4 volume set from 1984. ✓In 2012 – 5 volume set. ✓ Currently Bergey's Manual of Systematics of Archaea and Bacteria (2015), an online book, replaces the five- volume set.
- 27. In the original4 volumes of Bergey’s Manual of Systematic Bacteriology, bacteria were classified in 33 sections based on phenotypic, not phylogenetic characteristics. Kingdom: Procaryotae Divisions: ➢ Gracilicutes (thin skin): Procaryotes with a Gram- negative cell wall. ➢ Firmicutes (thick skin): Procaryotes with a Gram- positive cell wall. ➢ Tenericutes (soft or tender skin): Procaryotes that lack a cell wall. ➢ Mendosicutes (skin with faults): Procaryotes with unusual cell wall. The current 5 volumes define taxa not in terms of phenotype, but solely on 16S phylogeny