Bioethanol from
Biomass Waste
Isroi (http://isroi.com)
Bioethanol
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Bioethanol
 Renewable
 Environmental friendly
 Not compete with food and
feed
 Unblended bioethanol fuel or
Blended with gasoline
C2H5OH + 3 O2 → 2 CO2 + 3 H2O + heat
First Generation Bioethanol
First Generation Bioethanol
Ethanol from sugar (sugarcane, etc) and amylum (starch, casava, corn, etc).
Second Generation Bioethanol
Why lignocellulose?
Why lignocellulose?
Glucose
Starch
Cellulose
Ethanol
Why lignocellulose?
 Abundant
 Renewable
 Not compete with
food and feet
Oil Palm Waste
Abundant and un-use
Mass Balance in Oil Palm Mill
1 ton FFB 230 kg EFB
Problems and Challenges
PurificationHydrolysis Fermentation
Ethanol
Waste
water
Lignocellulose
Pretreatment
Pretreatment of Lignocelullose
Mosier et al. (2005)
Pretreatment:
• Mechanical
• Physical
• Chemical
• Biological
• Combination
Pretreatments have as a goal
to improve the digestibility of
the lignocellulosic biomass
(Hendriks and Zeeman, 2008).
Advantages and Disadvantages
Advantages Disadvantages
• No chemical added • Take more times
• Less energy used • Need more places
• More environmental
friendly
Biological pretreatment of EFB by white-rot fungi
compared with physical/chemical pretreatment
White-rot Fungi
Enzymes produced by
lignocellulytic fungi:
• Laccase
• Mangan Peroxidase (Mn P)
• Lignin Peroxidase (Li P)
White-rot fungi are the only known organisms
that, to any extent, mineralize lignin to CO2
and water in pure culture (Gold and Alic, 1993)
Biological Pretreatment
Untreated After pretreatment
Visual Changes of OPEFB after pretreatment
MyDream
EFB ETHANOL
Thank You

Bioethanol from biomass waste