Light and scanning electron microscopy of the small intestine of goat (Capra hircus)
Said A. Hassan
Eid A. Moussa
Received: 03 January 2015 Accepted: 12 January 2015 Published: 30 January 2015
Abstract
Sections from duodenum, jejunum and ileum of local breeds of goat (Capra hircus) were
studied histologically using light and scanning electron microscopy. The wall of goat small
intestine is composed of typical layers: lamina epithelialis mucosae, lamina propria, lamina
muscularis mucosae, tunica submucosa, tunica muscularis and tunica serosa. Small intestine villi
were covered by a simple columnar epithelium with goblet cells and simple tubular glands, the
crypts of LieberkĂĽhn, containing paneth cells were observed between the villi. The presence of
mucus was extensive in the duodenum and the number of goblet cells was highest in the duodenum.
The lamina propria consisted of loose connective tissue rich in blood and lymphatic vessels. The
lamina muscularis mucosa was presented as a thin layer of circular smooth muscle fibers at the
base of the crypts. The submucosa consisted of conjunctive tissue containing blood and lymphatic
vessels. The tunica muscularis consisted of the typical inner circular layer and outer longitudinal
layer of smooth muscle. The tunica serosa was the thin, outermost small intestine layer that
consisted of a very small amount of conjunctive tissue covered by mesothelium. The submucosa
was devoid of glands in all three small intestine regions. Scanning electron microscopy showed
finger shaped villi in the jujenum, tongue shaped in ileum, leave like in duodenum; the villus has
corrugated surface. The corrugations are deep, irregular clefts cutting into the side of the villus.
The corrugations are scarce in the duodenum, few in jujenum and numerous in ileum. The surface
of villi presents the goblet cell orifices.
Shaina Mavreen D. Villaroza Bio 108 Section: T45
Light and scanning electron microscopy of the small intestine of goat (Capra hircus)
Summary
There is little information about the histologic structure of goat small intestine, so
the current study was carried out to provide basic information concerning the goat small
intestine for anatomists and nutritionists. Randomly taken parts from duodenum, jejunum
and ileum of ten healthy freshly slaughtered adult goats were collected. Sections of
intestine underwent preservation and staining process (full details described in the
Materials and Methods of the study), different for light microscopy and scanning electron
microscopy. Under light microscopy, it was found that the wall of the goat small intestine
is composed of the typical layers found in mammals. Simple columnar cells were found in
the mucosal epithelium in all three small intestine segments, with goblet cells scattered
among them. Bifurcated and trifurcated villi which consisted of tongue-like elevations of
mucosa were observed. The ileum had the shortest villi and the least number of goblet cells.
Under scanning electron microscopy, corrugated surface of the jejunal villi were observed,
which is not easily seen by dissecting microscope. These corrugations were scarce in
duodenum, more frequent in the jejunal villi and most numerous in ileal villi. Mucus
extruding from goblet cells was frequently observed. These findings are similar to those
reported by David (1987) for human small intestine. Among villi, simple tubular glands
were observed, the crypts of Lieberkuhn, extending down into the lamina propria to the
level of the lamia muscularis mucosae. These crypts contain paneth cells, which have large
acidophilic cytoplasmic granules. These findings are similar to that of Daivid (1987) and
Leslie and James (2007) in both humans and animals.

Cell biology journal abstract and summary

  • 1.
    Light and scanningelectron microscopy of the small intestine of goat (Capra hircus) Said A. Hassan Eid A. Moussa Received: 03 January 2015 Accepted: 12 January 2015 Published: 30 January 2015 Abstract Sections from duodenum, jejunum and ileum of local breeds of goat (Capra hircus) were studied histologically using light and scanning electron microscopy. The wall of goat small intestine is composed of typical layers: lamina epithelialis mucosae, lamina propria, lamina muscularis mucosae, tunica submucosa, tunica muscularis and tunica serosa. Small intestine villi were covered by a simple columnar epithelium with goblet cells and simple tubular glands, the crypts of LieberkĂĽhn, containing paneth cells were observed between the villi. The presence of mucus was extensive in the duodenum and the number of goblet cells was highest in the duodenum. The lamina propria consisted of loose connective tissue rich in blood and lymphatic vessels. The lamina muscularis mucosa was presented as a thin layer of circular smooth muscle fibers at the base of the crypts. The submucosa consisted of conjunctive tissue containing blood and lymphatic vessels. The tunica muscularis consisted of the typical inner circular layer and outer longitudinal layer of smooth muscle. The tunica serosa was the thin, outermost small intestine layer that consisted of a very small amount of conjunctive tissue covered by mesothelium. The submucosa was devoid of glands in all three small intestine regions. Scanning electron microscopy showed finger shaped villi in the jujenum, tongue shaped in ileum, leave like in duodenum; the villus has corrugated surface. The corrugations are deep, irregular clefts cutting into the side of the villus. The corrugations are scarce in the duodenum, few in jujenum and numerous in ileum. The surface of villi presents the goblet cell orifices.
  • 2.
    Shaina Mavreen D.Villaroza Bio 108 Section: T45 Light and scanning electron microscopy of the small intestine of goat (Capra hircus) Summary There is little information about the histologic structure of goat small intestine, so the current study was carried out to provide basic information concerning the goat small intestine for anatomists and nutritionists. Randomly taken parts from duodenum, jejunum and ileum of ten healthy freshly slaughtered adult goats were collected. Sections of intestine underwent preservation and staining process (full details described in the Materials and Methods of the study), different for light microscopy and scanning electron microscopy. Under light microscopy, it was found that the wall of the goat small intestine is composed of the typical layers found in mammals. Simple columnar cells were found in the mucosal epithelium in all three small intestine segments, with goblet cells scattered among them. Bifurcated and trifurcated villi which consisted of tongue-like elevations of mucosa were observed. The ileum had the shortest villi and the least number of goblet cells. Under scanning electron microscopy, corrugated surface of the jejunal villi were observed, which is not easily seen by dissecting microscope. These corrugations were scarce in duodenum, more frequent in the jejunal villi and most numerous in ileal villi. Mucus extruding from goblet cells was frequently observed. These findings are similar to those reported by David (1987) for human small intestine. Among villi, simple tubular glands were observed, the crypts of Lieberkuhn, extending down into the lamina propria to the level of the lamia muscularis mucosae. These crypts contain paneth cells, which have large acidophilic cytoplasmic granules. These findings are similar to that of Daivid (1987) and Leslie and James (2007) in both humans and animals.